ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0302 good L KOG2994 Replication, recombination and repair Uracil DNA glycosylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0302 100414 101427 338
(338 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML021c [L] KOG2994 Uracil DNA glycosylase 347 1e-95
Hs6224979 [L] KOG2994 Uracil DNA glycosylase 227 2e-59
Hs19718751 [L] KOG2994 Uracil DNA glycosylase 227 2e-59
SPCC1183.06 [L] KOG2994 Uracil DNA glycosylase 224 1e-58
CE22594 [L] KOG2994 Uracil DNA glycosylase 181 1e-45
At3g18630 [L] KOG2994 Uracil DNA glycosylase 171 1e-42
ECU10g0530 [L] KOG2994 Uracil DNA glycosylase 161 1e-39
>YML021c [L] KOG2994 Uracil DNA glycosylase
Length = 359
Score = 347 bits (891), Expect = 1e-95
Identities = 175/264 (66%), Positives = 204/264 (76%), Gaps = 11/264 (4%)
Query: 84 KCLLDLEMDTIDISWLRRLKQEFSKPYFLKLKEFVVNEQKNCTVFPSPKDIYSWTRLTHF 143
+ LL LE++TID SW L EF KPYF+KLK+FV EQ + TVFP KDIYSWTRLT F
Sbjct: 92 RTLLSLELETIDDSWFPHLMDEFKKPYFVKLKQFVTKEQADHTVFPPAKDIYSWTRLTPF 151
Query: 144 DDVRVVIIGQDPYHNFNQAHGLAFSVRSPTPAPPSLKNIYKELQLNNYSDFKVNYSYGDL 203
+ V+VVIIGQDPYHNFNQAHGLAFSV+ PTPAPPSLKNIYKEL+ Y DF + GDL
Sbjct: 152 NKVKVVIIGQDPYHNFNQAHGLAFSVKPPTPAPPSLKNIYKELK-QEYPDFVEDNKVGDL 210
Query: 204 SSWSKQGVLLLNTSLTVKAHSANSHSKRGWEQFTKRAIDLLIEDRMKNGKPIVFLLWGNN 263
+ W+ QGVLLLNTSLTV+AH+ANSHSK GWE FTKR + LLI+DR +GK +VFLLWGNN
Sbjct: 211 THWASQGVLLLNTSLTVRAHNANSHSKHGWETFTKRVVQLLIQDREADGKSLVFLLWGNN 270
Query: 264 AIKLIE--------GEKRHLPSNFKVLKSVHPSPLSASRGFFGNKHFKIINDYLFENK-E 314
AIKL+E G P N V+KSVHPSPLSASRGFFG HFK+IND+L+ + E
Sbjct: 271 AIKLVESLLGSTSVGSGSKYP-NIMVMKSVHPSPLSASRGFFGTNHFKMINDWLYNTRGE 329
Query: 315 PMIDWSVVPGSTLQEVSEANKKLE 338
MIDWSVVPG++L+EV EAN +LE
Sbjct: 330 KMIDWSVVPGTSLREVQEANARLE 353
>Hs6224979 [L] KOG2994 Uracil DNA glycosylase
Length = 304
Score = 227 bits (579), Expect = 2e-59
Identities = 117/226 (51%), Positives = 152/226 (66%), Gaps = 15/226 (6%)
Query: 97 SWLRRLKQEFSKPYFLKLKEFVVNEQKNCTVFPSPKDIYSWTRLTHFDDVRVVIIGQDPY 156
SW + L EF KPYF+KL FV E+K+ TV+P P +++WT++ DV+VVI+GQDPY
Sbjct: 88 SWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPY 147
Query: 157 HNFNQAHGLAFSVRSPTPAPPSLKNIYKELQLNNYSDFKVNYSYGDLSSWSKQGVLLLNT 216
H NQAHGL FSV+ P P PPSL+NIYKEL + DF V+ +GDLS W+KQGVLLLN
Sbjct: 148 HGPNQAHGLCFSVQRPVPPPPSLENIYKELS-TDIEDF-VHPGHGDLSGWAKQGVLLLNA 205
Query: 217 SLTVKAHSANSHSKRGWEQFTKRAIDLLIEDRMKNGKPIVFLLWGNNAIK---LIEGEKR 273
LTV+AH ANSH +RGWEQFT D ++ +N +VFLLWG+ A K I+ ++
Sbjct: 206 VLTVRAHQANSHKERGWEQFT----DAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRH 261
Query: 274 HLPSNFKVLKSVHPSPLSASRGFFGNKHFKIINDYLFENKEPMIDW 319
H VL++ HPSPLS RGFFG +HF N+ L ++ + IDW
Sbjct: 262 H------VLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDW 301
>Hs19718751 [L] KOG2994 Uracil DNA glycosylase
Length = 313
Score = 227 bits (579), Expect = 2e-59
Identities = 117/226 (51%), Positives = 152/226 (66%), Gaps = 15/226 (6%)
Query: 97 SWLRRLKQEFSKPYFLKLKEFVVNEQKNCTVFPSPKDIYSWTRLTHFDDVRVVIIGQDPY 156
SW + L EF KPYF+KL FV E+K+ TV+P P +++WT++ DV+VVI+GQDPY
Sbjct: 97 SWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPY 156
Query: 157 HNFNQAHGLAFSVRSPTPAPPSLKNIYKELQLNNYSDFKVNYSYGDLSSWSKQGVLLLNT 216
H NQAHGL FSV+ P P PPSL+NIYKEL + DF V+ +GDLS W+KQGVLLLN
Sbjct: 157 HGPNQAHGLCFSVQRPVPPPPSLENIYKELS-TDIEDF-VHPGHGDLSGWAKQGVLLLNA 214
Query: 217 SLTVKAHSANSHSKRGWEQFTKRAIDLLIEDRMKNGKPIVFLLWGNNAIK---LIEGEKR 273
LTV+AH ANSH +RGWEQFT D ++ +N +VFLLWG+ A K I+ ++
Sbjct: 215 VLTVRAHQANSHKERGWEQFT----DAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRH 270
Query: 274 HLPSNFKVLKSVHPSPLSASRGFFGNKHFKIINDYLFENKEPMIDW 319
H VL++ HPSPLS RGFFG +HF N+ L ++ + IDW
Sbjct: 271 H------VLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDW 310
>SPCC1183.06 [L] KOG2994 Uracil DNA glycosylase
Length = 322
Score = 224 bits (571), Expect = 1e-58
Identities = 125/265 (47%), Positives = 166/265 (62%), Gaps = 20/265 (7%)
Query: 69 NKLKNAILQFVPEE--------KKCLLDLEMDTIDISWLRRLKQEFSKPYFLKLKEFVVN 120
NK N++ +F E+ ++ LL LE+DT++ SW LK EF KPYFL LKEF++
Sbjct: 49 NKENNSVSKFNKEKWAENLTPAQRKLLQLEIDTLESSWFDALKDEFLKPYFLNLKEFLMK 108
Query: 121 EQKNCTVFPSPKDIYSWTRLTHFDDVRVVIIGQDPYHNFNQAHGLAFSVRSPTPAPPSLK 180
E ++ VFP +DIYSW+ T +V+++GQDPYHN QAHGL FSVR P PPSL
Sbjct: 109 EWQSQRVFPPKEDIYSWSHHTPLHKTKVILLGQDPYHNIGQAHGLCFSVRPGIPCPPSLV 168
Query: 181 NIYKELQLNNYSDFKVNYSYGDLSSWSKQGVLLLNTSLTVKAHSANSHSKRGWEQFTKRA 240
NIYK +++ +Y DF + + G L W+ QG+L+LN SLTV+AH A SHS +GWE FT
Sbjct: 169 NIYKAIKI-DYPDFVIPKT-GYLVPWADQGILMLNASLTVRAHQAASHSGKGWETFTSAV 226
Query: 241 IDLLIEDRMKNGKPIVFLLWGNNAIKLIEGEKRHLP-SNFKVLKSVHPSPLSASRGFFGN 299
+ + + +N K +V L WG A K ++G LP VL+SVHPSPLSA RGFF
Sbjct: 227 LQVALN---RNRKGLVILAWGTPAAKRLQG----LPLKAHYVLRSVHPSPLSAHRGFFEC 279
Query: 300 KHFKIINDYLFE--NKEPMIDWSVV 322
HFK N++L E E I+WS V
Sbjct: 280 HHFKKTNEWLEEQYGPEKCINWSAV 304
>CE22594 [L] KOG2994 Uracil DNA glycosylase
Length = 282
Score = 181 bits (459), Expect = 1e-45
Identities = 105/227 (46%), Positives = 145/227 (63%), Gaps = 14/227 (6%)
Query: 97 SWLRRLKQEFSKPYFLKLKEFVVNE-QKNCTVFPSPKDIYSWTRLTHFDDVRVVIIGQDP 155
SW + L++EF K Y K+++F+ +E K VFP P I++ L FD++ VVIIGQDP
Sbjct: 65 SWSKLLEEEFKKGYISKIEKFLNSEVNKGKQVFPPPTQIFTTFNLLPFDEISVVIIGQDP 124
Query: 156 YHNFNQAHGLAFSVRSPTPAPPSLKNIYKELQLNNYSDFKVNYSYGDLSSWSKQGVLLLN 215
YH+ NQAHGL+FSV+ PPSLKNIYKEL+ ++ FK +G+L W++QGV +LN
Sbjct: 125 YHDDNQAHGLSFSVQKGVKPPPSLKNIYKELE-SDIEGFK-RPDHGNLLGWTRQGVFMLN 182
Query: 216 TSLTVKAHSANSHSKRGWEQFTKRAIDLLIEDRMKNGKPIVFLLWGNNAIKLIE--GEKR 273
+LTV+AH ANSH+K GW+ FT I ++ ++ KPIVFLLWG A K E K+
Sbjct: 183 ATLTVRAHEANSHAKIGWQTFTDTVIRII---SRQSEKPIVFLLWGGFAHKKEELIDTKK 239
Query: 274 HLPSNFKVLKSVHPSPLSASRGFFGNKHFKIINDYLFENKEPMIDWS 320
H+ V+K+ HPSPLSA R ++G K F N L + I+W+
Sbjct: 240 HV-----VIKTAHPSPLSA-RKWWGCKCFSKCNTELENSGRNPINWA 280
>At3g18630 [L] KOG2994 Uracil DNA glycosylase
Length = 330
Score = 171 bits (434), Expect = 1e-42
Identities = 102/247 (41%), Positives = 137/247 (55%), Gaps = 20/247 (8%)
Query: 82 EKKCLLDLEMDTIDISWLRRLKQEFSKPYFLKLKEF----VVNEQKNCTVFPSPKDIYSW 137
E C + L ++ SWL+ L EF KPY L +F ++ + K+ ++P I++
Sbjct: 97 EGNCYVPLSELLVEESWLKALPGEFHKPYAKSLSDFLEREIITDSKSPLIYPPQHLIFNA 156
Query: 138 TRLTHFDDVRVVIIGQDPYHNFNQAHGLAFSVRSPTPAPPSLKNIYKELQLNNYSDFKVN 197
T FD V+ VIIGQDPYH QA GL+FSV P SL NI+KEL +
Sbjct: 157 LNTTPFDRVKTVIIGQDPYHGPGQAMGLSFSVPEGEKLPSSLLNIFKELHKDVGCSIP-- 214
Query: 198 YSYGDLSSWSKQGVLLLNTSLTVKAHSANSHSKRGWEQFTKRAIDLLIEDRMKNGKPIVF 257
+G+L W+ QGVLLLN LTV++ NSH+K+GWEQFT D +I+ + + +VF
Sbjct: 215 -RHGNLQKWAVQGVLLLNAVLTVRSKQPNSHAKKGWEQFT----DAVIQSISQQKEGVVF 269
Query: 258 LLWGNNA---IKLIEGEKRHLPSNFKVLKSVHPSPLSASRGFFGNKHFKIINDYLFENKE 314
LLWG A KLI+ K H +L + HPS LSA+RGFF +HF N L E
Sbjct: 270 LLWGRYAQEKSKLIDATKHH------ILTAAHPSGLSANRGFFDCRHFSRANQLLEEMGI 323
Query: 315 PMIDWSV 321
P IDW +
Sbjct: 324 PPIDWQL 330
>ECU10g0530 [L] KOG2994 Uracil DNA glycosylase
Length = 286
Score = 161 bits (408), Expect = 1e-39
Identities = 102/233 (43%), Positives = 137/233 (58%), Gaps = 19/233 (8%)
Query: 92 DTIDISWLRRLKQEFSKPYFLKLKEFVVNEQKNCTVFPSPKDIYSWTRLTHFDDVRVVII 151
+ ID W LK EF K YF +K F+ +N P I++++R + +VVI+
Sbjct: 70 EKIDPRWRMHLKPEFGKDYFRGVKRFL---HENRNHLPPIDKIFTFSRFFPLEKTKVVIM 126
Query: 152 GQDPYHNFNQAHGLAFSVRSPTPAPPSLKNIYKELQLNNYSDFKVNYSYGDLSSWSKQGV 211
GQDPYHN NQA GL+FSV P PPSLKNIY E+ ++ F+V S+GDLS W+++GV
Sbjct: 127 GQDPYHNDNQAMGLSFSVPVGIPVPPSLKNIYLEIS-SDIEGFEVP-SHGDLSGWAERGV 184
Query: 212 LLLNTSLTVKAHSANSHSKRGWEQFTKRAIDLLIEDRMKNGKPIVFLLWGNNAI---KLI 268
LLLN LTV + NSH+ GW +FT I +I ++ N +VF+LWG +A K I
Sbjct: 185 LLLNDVLTVTKNRPNSHAGIGWREFTS-CILQVINEKCTN---VVFMLWGRHAQAKGKFI 240
Query: 269 EGEKRHLPSNFKVLKSVHPSPLSASRGFFGNKHFKIINDYLFENKEPMIDWSV 321
K HL VL HPSPLS+S FFG KHF N YL ++ + ++W V
Sbjct: 241 NRNK-HL-----VLTCGHPSPLSSSH-FFGCKHFSKANKYLMDHGKSPVEWQV 286
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.132 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,455,980
Number of Sequences: 60738
Number of extensions: 851691
Number of successful extensions: 2056
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2025
Number of HSP's gapped (non-prelim): 7
length of query: 338
length of database: 30,389,216
effective HSP length: 107
effective length of query: 231
effective length of database: 23,890,250
effective search space: 5518647750
effective search space used: 5518647750
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)