ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0333 good R KOG4372 General function prediction only Predicted alpha/beta hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0333 110709 112628 640
(640 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR444w [R] KOG4372 Predicted alpha/beta hydrolase 592 e-169
YGL144c [R] KOG4372 Predicted alpha/beta hydrolase 301 2e-81
YDL109c [R] KOG4372 Predicted alpha/beta hydrolase 286 7e-77
SPAC4A8.10 [R] KOG4372 Predicted alpha/beta hydrolase 140 8e-33
>YDR444w [R] KOG4372 Predicted alpha/beta hydrolase
Length = 687
Score = 592 bits (1527), Expect = e-169
Identities = 307/650 (47%), Positives = 426/650 (65%), Gaps = 25/650 (3%)
Query: 8 ENDTESSDSG-ILLLRETGSLGIGDVNRYKIIVDKNKLLSDGIECTDDTLYLEFKNQESA 66
+N E +++G LL+ E L IG++ RYK V+K+ + G++ + L+L KN+ESA
Sbjct: 9 KNSLEINETGGTLLVDERKRLRIGELYRYKFSVNKDVIKEQGLDVSH--LFLRIKNEESA 66
Query: 67 LLRPLWITGPYSIYVEITPNNYDERKQFIG-DGIEFMSDLKPDENFKAKLYLNSNARVDG 125
LLRPL++TGPYS Y+++ P+NY+E ++F G + I F+ +LKPDE FK K+ LN N+RV
Sbjct: 67 LLRPLYLTGPYSFYIDVRPHNYNENRKFPGKEIIPFVENLKPDERFKVKILLNENSRVGD 126
Query: 126 TSCYAWKIDLIAQFTVVTIVRLPFVFTLATTYKTAKHSQKDKNIEVQQTDGLKISKLDTE 185
TS Y+W ID+I+Q V TI +L F F + TT K K S N + +G+ + DTE
Sbjct: 127 TSLYSWTIDIISQLAVTTIPKLEFSFRIGTTRKVVKKS----NGLFKSIEGVSLEMWDTE 182
Query: 186 QLWNLPPPFPDKPVHLVIITHGIFSSIGGDMLCLKDTIERASNFLPDDNNGNLVIRGYPG 245
LW+LPP FP+KPVHLVI+THGIFS+IG DML +KD IE + + + N N+++RG+ G
Sbjct: 183 TLWDLPPKFPEKPVHLVIMTHGIFSNIGCDMLYMKDKIEEMTFPMDESINPNIIVRGFMG 242
Query: 246 NVGKSHKGIRHLGFKLAEYIIDTIDKLQQQFTLTRISFVGHSLGGPVQAMAIHYISVERP 305
NVGKS GI LG ++ +Y+++T+DKL +++ + RISF+GHSLGGP Q+MA+ YIS++RP
Sbjct: 243 NVGKSGHGIHCLGVRVGKYVLETVDKLNKKYKVDRISFIGHSLGGPTQSMAVRYISIKRP 302
Query: 306 DIFDKTTGLTPVNFVAAASPFLGVIGDLPKYISIVLDIGALGQTGRDLTLKRSYFLPSKG 365
D FD G+ PVNF+ ASPF+GVIGD P Y+S+ LD+GALG TGRDL LK + G
Sbjct: 303 DFFDAVKGVKPVNFITLASPFIGVIGDFPFYLSVPLDMGALGLTGRDLNLKYTPLTSKDG 362
Query: 366 IVNNDGSHDRIKSKPILELLPKHPALEVFQRFKCRTVYANVAFDGIVPLRTAALLYLDWR 425
+ +D + SK ILE+LP+ PA +VF+ FK RT+YANV DGIVPLRTAALLYLDWR
Sbjct: 363 LYADDEVYPE-HSKYILEILPQAPAKKVFESFKRRTIYANVMDDGIVPLRTAALLYLDWR 421
Query: 426 GLSDVQQVR--SENNAQSEEGV-------------EEQKGSSLGEIPESSSDNKSILQWL 470
+ VQ++R ++N+ S E V + G+++GEIP S + K+ LQW
Sbjct: 422 SIHKVQKIRKKNKNSPTSSEFVSSDSPESSGASSPSNENGNNVGEIPAESPNKKATLQWT 481
Query: 471 LPQSLIKKEKYKPYVRXXXXXXXXXXXXXXXXXXXXXXXFKPPKKANTLQAAASTISAPL 530
LPQ++I K Y R F+PPK+ANT+ +A S ++A +
Sbjct: 482 LPQAVIHGSKINKYKR-GQTNEANSDSDNEQGVFLDGQKFEPPKEANTVLSALSVLTAAI 540
Query: 531 PGMSYLVDPSSRTDRIIHDKVYTPEELPDXXXXXXXXXXXXXXPNYSIHMKEERIARYWQ 590
P Y+ +P+ R D +IHDK+Y PEELP PN S++ +ERIAR WQ
Sbjct: 541 PDQEYIKNPAVRKDEVIHDKLYHPEELPPPHYENRPIVKKLIYPNESVNRIQERIAREWQ 600
Query: 591 ETMDWRKVIVELQPDSHNNIIVRRRFVNSFGWIVVNHIADEHFGSKAMIE 640
ETM WRKV+V++QPDSHNNI+VRRRFVN +G++ V H+ + HFGSK E
Sbjct: 601 ETMTWRKVLVQIQPDSHNNIVVRRRFVNLYGYVAVEHMVEHHFGSKVCSE 650
>YGL144c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 685
Score = 301 bits (772), Expect = 2e-81
Identities = 198/635 (31%), Positives = 317/635 (49%), Gaps = 85/635 (13%)
Query: 19 LLLRETGSLGIGDVNRYKIIVDKNKLLSDGIECTDD----TLYLEFKNQESALLRPLWIT 74
+L S+ +G++ RY I L DG E D +L+L+ +N R ++
Sbjct: 9 ILFHYKSSVKVGELERYVITYH----LYDGEEIPPDLNLNSLWLKVRNMNPLSYRAAYLM 64
Query: 75 GPYSIYVEITPNNYDERKQFIG--DGIEFMSDLKPDENFKAKLYLNSNARVDGTSCYAWK 132
GP+ +Y ++ Y ++ + D +F +++ ++F A+L +++ + Y W
Sbjct: 65 GPFMLYCDVKTAQYHHSQKIVASVDYPKFEPNVQTQQDFVAELSVHNIRQK-----YVWI 119
Query: 133 IDLIAQFTVVTIVRLPFVFTLATTYKTAKHSQKDKNIEVQQTDGLKISKLDTEQLWNLPP 192
D+++Q T + + T+ T+ ++ ++ + + L +++L T LWNLP
Sbjct: 120 ADVMSQILFTTNTNVTYEVTIGTSKESVENPHDLPSHLGSYSPKLTVNRLTTLDLWNLPV 179
Query: 193 PF--PDKPVHLVIITHGIFSSIGGDMLCLKDTIERASNFLPDDNNGNLVIRGYPGNVGKS 250
P K HLV++THG+ S++ D++ + + I +A P + +V++GY GNV ++
Sbjct: 180 QITTPQKKKHLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQ---IVVKGYRGNVCQT 236
Query: 251 HKGIRHLGFKLAEYII-DTIDKLQQQFTLTRISFVGHSLGGPVQAMAIHYISVERPDIFD 309
KG+++LG +LAEYII D D+ ++ +ISFVGHSLGG +QA AI YI P F
Sbjct: 237 EKGVKYLGTRLAEYIIQDLYDE-----SIRKISFVGHSLGGLIQAFAIAYIYEVYPWFFK 291
Query: 310 KTTGLTPVNFVAAASPFLGVIGDLPKYISIVLDIGALGQTGRDLTLKRSYFLPSKGIVNN 369
K + P+NF+ ASP LG++ D P YI ++L G +G+TG+DL L+
Sbjct: 292 K---VNPINFITLASPLLGIVTDNPAYIKVLLSFGVIGKTGQDLGLE------------- 335
Query: 370 DGSHDRIKSKPILELLPKHPALEVFQRFKCRTVYANVAFDGIVPLRTAALLYLDWRGLSD 429
+D KP+L LL P +E+ +RFK RTVYAN DGIVPL TA+LL+LD+ + +
Sbjct: 336 ---NDVEVGKPLLYLLSGLPLIEILRRFKRRTVYANAINDGIVPLYTASLLFLDYNDILE 392
Query: 430 VQQVRSENNAQSEEGVEEQKGSSLGEIPESSSDNKSILQWLLPQSLIKKEKYKPYVRXXX 489
Q EN+ +S I ++S+ + Q K P +
Sbjct: 393 QLQKLKENSKKSP------------LINDASTP--------VNQDFFNKTFISPLTKMLS 432
Query: 490 XXXXXXXXXXXXXXXXXXXXFKPPKKANTLQAAASTISAPLPGMSYLVDPSSRTDRIIHD 549
K + ++A+S + PLP +Y++DP SR IIHD
Sbjct: 433 ILAPQKFPTENGSEI---------PKVSFFESASSILLPPLPERAYIMDPDSRDPVIIHD 483
Query: 550 KVYTPEELPDX-----------XXXXXXXXXXXXXPNYSIHMKEERIARYWQETMDWRKV 598
K+Y +++P EE IAR W E M WRKV
Sbjct: 484 KIYNEDDIPQSEFDIEDGFFGKKNILLQAFFAGKKERAKYRNLEETIARRWHEGMAWRKV 543
Query: 599 IVELQPDSHNNIIVRRRFVNSFGWIVVNHIADEHF 633
+V L+PD+HNNIIVRR+F N++GW V++H+ D HF
Sbjct: 544 VVALKPDAHNNIIVRRKFANAYGWPVIDHLIDVHF 578
>YDL109c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 647
Score = 286 bits (732), Expect = 7e-77
Identities = 198/630 (31%), Positives = 309/630 (48%), Gaps = 76/630 (12%)
Query: 19 LLLRETGSLGIGDVNRYKIIVDKNKLLSDGIECTDDTLYLEFKNQESALLRPLWITGPYS 78
+L S+ +G++ RY I + + S + T D+L+++ KN +P ++ GP+
Sbjct: 10 VLYHNQSSVKLGELERYVITYELYQGDSIPADITLDSLWVKIKNTTKLSYKPAYLLGPFI 69
Query: 79 IYVEITPNNYDERKQFI--GDGIEFMSDLKPDENFKAKLYLNSNARVDGTSCYAWKIDLI 136
+Y ++ +Y+ + I D F S+L+ + F A+L L+ Y W +D++
Sbjct: 70 LYCDVRAKDYESSYKIICSADKPVFQSNLQAQQKFIAELSLHHIK-----PRYVWIVDIV 124
Query: 137 AQFTVVTIVRLPFVFTLATTYKTAKH------SQKDKNIEVQQTDGLKISKLDTEQLWNL 190
+Q ++ F + + + K S DK + T GL + +L T +W +
Sbjct: 125 SQILFNKETKVTFEIVVGNSKASLKRKIRCNDSLPDKACNILHT-GLSVHRLTTADIWKV 183
Query: 191 PPPFP-DKPVHLVIITHGIFSSIGGDMLCLKDTIERASNFLPDDNNGNLVIRGYPGNVGK 249
P P + HLVI+THG S++ DM L + I +A P N LVI+GY N +
Sbjct: 184 PRPIDMSQKSHLVILTHGFQSNVSADMEYLMEEIYKAQMNNP---NERLVIKGYMKNACE 240
Query: 250 SHKGIRHLGFKLAEYIIDTIDKLQQQFTLTRISFVGHSLGGPVQAMAIHYISVERPDIFD 309
+ KGI+ LG LA YIID + ++ +ISF+GHSLGG Q AI YI + P F
Sbjct: 241 TEKGIKFLGVGLANYIIDEL----YDDSVGKISFIGHSLGGLTQTFAICYIKTKYPYFFK 296
Query: 310 KTTGLTPVNFVAAASPFLGVIGDLPKYISIVLDIGALGQTGRDLTLKRSYFLPSKGIVNN 369
K + P+NF++ ASP LG+ P Y+ + L +G +G TG++L LK
Sbjct: 297 K---VEPINFISLASPLLGIATSTPNYVKMSLSMGIIGTTGQELGLK------------- 340
Query: 370 DGSHDRIKSKPILELLPKHPALEVFQRFKCRTVYANVAFDGIVPLRTAALLYLDWRGLSD 429
DG++ KP+L LL + + V RFK RT+YAN DGIVPL +++LL+LD+ L
Sbjct: 341 DGNYG---DKPLLYLLSEESLISVLARFKRRTLYANAVNDGIVPLYSSSLLFLDYSQL-- 395
Query: 430 VQQVRSENNAQSEEGVEEQKGSSLGEIPESS-----SDNKSILQWLLPQSLIKKEKYKPY 484
+Q++ + A + + + + S +GE+P S D + W + K K
Sbjct: 396 LQKLGGQTTAPCDP-LFQPEVSPIGELPNHSDVANDDDGINASSW----NTFWKSKENNC 450
Query: 485 VRXXXXXXXXXXXXXXXXXXXXXXXFKPPKKANTLQAAASTISAPLPGMSYLVDPSSRTD 544
+ K A+ +++ S + +P P + DP +R
Sbjct: 451 DKKS----------------------KRLMNASVIKSMKSVLLSPCPDAKFFSDPDARVA 488
Query: 545 RIIHDKVYTPEELPDXXXXXXXXXXXXXXPNYSIHMK-EERIARYWQETMDWRKVIVELQ 603
IIHDK+YT + LP + EE IAR W + M WRKV+V L+
Sbjct: 489 TIIHDKIYTEKNLPPPSPTLYEGTAAKEGETRKTRKEMEEIIARRWHKGMHWRKVVVLLK 548
Query: 604 PDSHNNIIVRRRFVNSFGWIVVNHIADEHF 633
PD+HNNIIVRRRF N++GW VV+H+ HF
Sbjct: 549 PDAHNNIIVRRRFSNAYGWPVVDHLVTAHF 578
>SPAC4A8.10 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 723
Score = 140 bits (352), Expect = 8e-33
Identities = 110/420 (26%), Positives = 192/420 (45%), Gaps = 45/420 (10%)
Query: 49 IECTDDTLYLEFKNQESALLRPLWITGPYSIYVEITPNNYDERKQFIGDGIEFMSDLKPD 108
I T+ L++ +N+E+ + ++ GP+++ V + + + + K + + F ++P
Sbjct: 101 INSTNSVLWIRVRNREARFRQAAYLQGPFTLCVSVWNDQFFDEKNSL---LNFDPQVQPS 157
Query: 109 ENFKAKLYLNSNARVDGTSCYAWKIDLIAQFTVVTIVR---LPFVFTLATTYKTAKHSQK 165
+F + SC++ + + + I + + F ++ + + +
Sbjct: 158 TSFWVCVPY---------SCFSNQQPIYIEIASQAIFKDRSINFELAISQSQHSIRAMSA 208
Query: 166 DKNIEVQQTDGLKISKLDTEQLWNLP-PPFPDKPVHLVIITHGIFSSIGGDMLCLKDTIE 224
+ L + E LW LP F K HLV++THG+ S++G DM LK+ +
Sbjct: 209 ADIDTLHAFPALHVEHQTPENLWRLPYSSFHSKNSHLVVLTHGMHSNVGADMEYLKEKLI 268
Query: 225 RASNFLPDDNNGNLVIRGYPGNVGKSHKGIRHLGFKLAEYIIDTIDKLQQQFT-LTRISF 283
+S + + +V+RG+ GN ++ KG+R LG +L E+++D F + IS
Sbjct: 269 ESSKSVKE----LVVVRGFTGNYCQTEKGVRWLGKRLGEWLLDITGWGSASFPRYSHISV 324
Query: 284 VGHSLGGPVQAMAIHYISVERPDIFDKTTGLTPVNFVAAASPFLGVIGDLPKYISIVLDI 343
V HSLGG VQ A+ Y+ + F + + PV FV A+P+LGV G+ P YI L
Sbjct: 325 VAHSLGGLVQTYAVGYVHAKTHGAFFQA--IHPVFFVTLATPWLGVAGEHPSYIGKALSY 382
Query: 344 GALGQTGRDLTLKRSYFLPSKGIVNNDGSHDRIKSKPILELL--PKHPALEVFQRFKCRT 401
G +G+TG+DL+L + I+S+P L L+ P P + F+ R
Sbjct: 383 GIIGKTGQDLSL--------------TPLNHSIESRPFLVLMSDPSTPFFQAVSFFEKRI 428
Query: 402 VYANVAFDGIVPLRTAAL------LYLDWRGLSDVQQVRSENNAQSEEGVEEQKGSSLGE 455
++AN D IVP T+A+ + G V EN EQ S+G+
Sbjct: 429 LFANTTNDYIVPFGTSAMEVSSLGKVEEAEGSDKVMPTHMENGISPTLKENEQTVQSVGD 488
Score = 55.5 bits (132), Expect = 3e-07
Identities = 24/49 (48%), Positives = 33/49 (66%), Gaps = 1/49 (2%)
Query: 582 EERIARYWQETMDWRKVIVELQPDSHNNIIVRRRFVNSFGWIVVNHIAD 630
E IA+ W + WRKV V L D+HN +IVRRRF N++GW V+ H+ +
Sbjct: 639 EITIAKNWHR-LAWRKVAVRLDGDAHNTMIVRRRFPNAYGWTVIKHLTE 686
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,134,783
Number of Sequences: 60738
Number of extensions: 1625710
Number of successful extensions: 3872
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3840
Number of HSP's gapped (non-prelim): 8
length of query: 640
length of database: 30,389,216
effective HSP length: 113
effective length of query: 527
effective length of database: 23,525,822
effective search space: 12398108194
effective search space used: 12398108194
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)