ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0362 good J KOG1624 Translation, ribosomal structure and biogenesis Mitochondrial/chloroplast ribosomal protein L4
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0362 Similar to YML025c SP|P51998 YML6 ribosomal
protein, mitochondrial singleton [INTRON]
(263 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML025c [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4 329 2e-90
SPBC2D10.08c [J] KOG1624 Mitochondrial/chloroplast ribosomal pro... 102 4e-22
At2g20060 [J] KOG1624 Mitochondrial/chloroplast ribosomal protei... 49 6e-06
7298296 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4 45 8e-05
At1g07320 [J] KOG1624 Mitochondrial/chloroplast ribosomal protei... 44 3e-04
CE14028 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4 35 0.11
Hs7705722 [J] KOG1624 Mitochondrial/chloroplast ribosomal protei... 35 0.14
YLR424w [A] KOG2184 Tuftelin-interacting protein TIP39 contains ... 34 0.18
At5g38560 [T] KOG1187 Serine/threonine protein kinase 32 0.92
At1g26170 [UY] KOG2274 Predicted importin 9 31 1.6
CE26551 [I] KOG2624 Triglyceride lipase-cholesterol esterase 31 2.0
7290247 [R] KOG3656 FOG: 7 transmembrane receptor 30 2.7
Hs6005701 [IR] KOG0059 Lipid exporter ABCA1 and related proteins... 30 4.6
At1g05910 [O] KOG0732 AAA+-type ATPase containing the bromodomain 30 4.6
7301301 [U] KOG0864 Ran-binding protein RANBP1 and related RanBD... 29 5.9
CE00373 [O] KOG2177 Predicted E3 ubiquitin ligase 29 7.8
At2g04190 [DR] KOG1987 Speckle-type POZ protein SPOP and related... 29 7.8
At1g64030 [V] KOG2392 Serpin 29 7.8
7298249 [A] KOG0948 Nuclear exosomal RNA helicase MTR4 DEAD-box ... 29 7.8
7297532 [R] KOG0231 Junctional membrane complex protein Junctoph... 29 7.8
>YML025c [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 286
Score = 329 bits (844), Expect = 2e-90
Identities = 159/246 (64%), Positives = 201/246 (81%), Gaps = 6/246 (2%)
Query: 22 PLPNASLPPKYTLATVRSFPSLEPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSS 81
PLPNA++PPKY L TVRSFPSLEPLTF PVPTS APLRRD+LWRAVVYEND++RVG+S
Sbjct: 31 PLPNAAIPPKYALVTVRSFPSLEPLTFVPVPTSTVAAPLRRDILWRAVVYENDNRRVGAS 90
Query: 82 NPPGRSDVGYSRRKILPQKGSGMARAGDANSPIRHNGGRALARTAPNDYTTALPKKVYSL 141
NPPGRS+ G+SRRK++PQKGSG AR GDANSP RHNGGRALARTAPNDYTT LP KVYS+
Sbjct: 91 NPPGRSENGFSRRKLMPQKGSGRARVGDANSPTRHNGGRALARTAPNDYTTELPSKVYSM 150
Query: 142 AFNNALSHQYRNGNVFVIGSG--DGISETSQADLNELEILPIG--EGSE--DGDIVFEKF 195
AFNNALSHQY++G +FVIG D IS T + DLN L+++ EG E +G+++F KF
Sbjct: 151 AFNNALSHQYKSGKLFVIGGEKVDLISPTPELDLNRLDLVNTNTVEGKEIFEGEVIFRKF 210
Query: 196 LKHYELQNKKLLFVINEPRPGLFSFTERFQDKVNIVQKEFVDVNDLLKAHRIFIELEALE 255
L+ ++L+ K+LLF+ ++ R GL ++ ++ KV+++QKE V+VND+L+A +FIELEALE
Sbjct: 211 LEEFQLKGKRLLFITDKTREGLIKSSDPYKQKVDVIQKELVEVNDILRAQAVFIELEALE 270
Query: 256 YLAVTH 261
YLA+ H
Sbjct: 271 YLAMAH 276
>SPBC2D10.08c [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 261
Score = 102 bits (255), Expect = 4e-22
Identities = 71/222 (31%), Positives = 115/222 (50%), Gaps = 18/222 (8%)
Query: 40 FPSLEPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSSNPPGRSDVGYSRRKILPQ 99
FPSLEP + +P S RRD+L RAV+YE D+ R G+++ R+++ S RK+ Q
Sbjct: 13 FPSLEPASLFLIPGSFLQERFRRDILHRAVIYEADNDRQGTASSKRRNEITCSGRKLYRQ 72
Query: 100 KGSGMARAGDANSPIRHNGGRALARTAPNDYTTALPKKVYSLAFNNALSHQYRNGNVFVI 159
KG+G AR GDA+SPIR G ++ P D++T L +VY+LA ALS ++ N + ++
Sbjct: 73 KGTGHARVGDASSPIRRGGAKSHG-PKPRDFSTKLQTQVYNLAMRIALSTRFINNELTIL 131
Query: 160 GSGDGISETSQADLNELEILPIGE-GSEDGDIVF---EKFLKHYELQNKKLLFVINEPRP 215
+ +S+ L LE+L + G E G +F + + + L+N LL
Sbjct: 132 ENPINLSQPKTRIL--LEVLKQHKLGHEYGKALFVLNDNYFEDESLKNNNLLLA------ 183
Query: 216 GLFSFTERFQDKVNIVQKEFVDVNDLLKAHRIFIELEALEYL 257
+ + + + V D LK ++FI+ EA+ +
Sbjct: 184 -----SRQLGYHCSFIPVGEFQVRDALKFGKLFIDSEAMSLI 220
>At2g20060 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 266
Score = 49.3 bits (116), Expect = 6e-06
Identities = 31/111 (27%), Positives = 53/111 (46%), Gaps = 1/111 (0%)
Query: 48 FAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSSNPPGRSDVGYSRRKILPQKGSGMARA 107
F + VFD P+R+D++ V ++ ++ G+ + S+V + RK QKG+G AR
Sbjct: 69 FMVLAGDVFDVPIRKDIIHNVVRWQLAKRQQGTHSTKTLSEVSGTGRKPWNQKGTGRARH 128
Query: 108 GDANSPIRHNGGRALARTAPNDYTTALPKKVYSLAFNNALSHQYRNGNVFV 158
G P + GG + P + + K+V L AL+ + G + V
Sbjct: 129 GTLRGP-QFRGGCVMHGPKPRSHAIKMNKQVRRLGLKIALTARAAEGKLLV 178
>7298296 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 296
Score = 45.4 bits (106), Expect = 8e-05
Identities = 31/109 (28%), Positives = 50/109 (45%)
Query: 55 VFDAPLRRDLLWRAVVYENDSKRVGSSNPPGRSDVGYSRRKILPQKGSGMARAGDANSPI 114
VF A R D++ V +++ + V ++ R++V RK PQKG G AR G SP+
Sbjct: 89 VFAAQPRVDIIQENVEWQSKYRYVSMAHTKTRAEVRGGGRKPWPQKGGGRARHGSLRSPM 148
Query: 115 RHNGGRALARTAPNDYTTALPKKVYSLAFNNALSHQYRNGNVFVIGSGD 163
GG +P + LP L + LS + ++ +I + D
Sbjct: 149 LKGGGVVHGPRSPTTHFYMLPFYKRVLGLTSTLSVKLAQDDLHIIDNVD 197
>At1g07320 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 282
Score = 43.5 bits (101), Expect = 3e-04
Identities = 28/87 (32%), Positives = 47/87 (53%), Gaps = 1/87 (1%)
Query: 62 RDLLWRAVVYENDSKRVGSSNPPGRSDVGYSRRKILPQKGSGMARAGDANSPIRHNGGRA 121
R ++ RA+V + ++KR G+++ R +V K QK +G AR G +P+R GG
Sbjct: 80 RAVVHRAIVTDLNNKRRGTASTLTRGEVRGGGIKPYSQKKTGHARRGSQRTPLRPGGG-V 138
Query: 122 LARTAPNDYTTALPKKVYSLAFNNALS 148
+ P D++ + +K LA + ALS
Sbjct: 139 VFGPRPKDWSIKINRKEKKLAISTALS 165
>CE14028 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 299
Score = 35.0 bits (79), Expect = 0.11
Identities = 27/116 (23%), Positives = 49/116 (41%)
Query: 44 EPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSSNPPGRSDVGYSRRKILPQKGSG 103
E L + +F R D+L R + +++ + V + ++++ RK PQK +G
Sbjct: 62 EKLGLVDLHPDIFRTSPRIDILHRNLTWQSVYRNVQMTKMLTKAEMPGGGRKPWPQKKTG 121
Query: 104 MARAGDANSPIRHNGGRALARTAPNDYTTALPKKVYSLAFNNALSHQYRNGNVFVI 159
A G SP GG A P + LP V AL+ ++ ++ ++
Sbjct: 122 RAHVGSIRSPQFIRGGFANGVRGPRTWFYMLPDAVRIQGLCVALTLKHTQDDLHIV 177
>Hs7705722 [J] KOG1624 Mitochondrial/chloroplast ribosomal protein L4
Length = 311
Score = 34.7 bits (78), Expect = 0.14
Identities = 54/248 (21%), Positives = 98/248 (38%), Gaps = 44/248 (17%)
Query: 22 PLPNASLPPKYTLATVRSFPSLEPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSS 81
P+P P + + ++R F E + A + VF R D+L + +++ + KR+ +
Sbjct: 54 PVPTHRRPVQAWVESLRGFEQ-ERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYA 112
Query: 82 NPPGRSDVGYSRRKILPQKGSGMARAGDAN--SPIRHNGGRALAR-------TAPNDYTT 132
R++V + G G A + + P + ALAR P Y
Sbjct: 113 KTKTRAEV---------RGGGGKPLAAERHWAGPAWQHPLSALARRRCCPWPPGPTSYYY 163
Query: 133 ALPKKVYSLAFNNALSHQYRNGNVFVIGSGDGISETSQADLNELEILPIGEGSEDGDIVF 192
LP KV +L AL+ + +Q DL+ ++ L + GD +
Sbjct: 164 MLPMKVRALGLKVALT-----------------VKLAQDDLHIMDSLEL----PTGDPQY 202
Query: 193 EKFLKHYELQNKKLLFV--INEPRP-GLFSFTERFQDKVNIVQKEFVDVNDLLKAHRIFI 249
L HY +L V +E P + T R + N++ ++V+ +LK + +
Sbjct: 203 LTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLK-TFNLIPAVGLNVHSMLKHQTLVL 261
Query: 250 ELEALEYL 257
L + +L
Sbjct: 262 TLPTVAFL 269
>YLR424w [A] KOG2184 Tuftelin-interacting protein TIP39 contains G-patch
domain
Length = 708
Score = 34.3 bits (77), Expect = 0.18
Identities = 43/195 (22%), Positives = 72/195 (36%), Gaps = 37/195 (18%)
Query: 69 VVYENDSKRVGSSNPPG--------------RSDVGYSRRKILPQKGSGMARAGDANSPI 114
+ Y ND+ + S N P S +GY K L + GSG+ + S
Sbjct: 38 MTYTNDALKTSSGNAPTISKLTKTYGIGAKLLSSMGYVAGKGLGKDGSGITTPIETQSRP 97
Query: 115 RHNGGRALARTAPNDYTTALPKKVYSLAFNNALSHQYRNGNVFVIGSGDGISE-TSQADL 173
HN G + F+N S Y + N + S D + E Q
Sbjct: 98 MHNAGLGM--------------------FSNTNSSNYHSENEDYLSSEDEVVEGIEQVKF 137
Query: 174 NELEILPIGEG--SEDGDIVFEKFLKHYELQNKKLLFVINEPRPGLFSFTERFQDKVNIV 231
N+ +GE ++ GD+ + L+ L +L I + L + + +D V +
Sbjct: 138 NKTSTEVLGEALLNDSGDMTIVRTLRELRLAGVQLPESILKELDPLNAVPKPKKDVVVEI 197
Query: 232 QKEFVDVNDLLKAHR 246
+E + + L+A R
Sbjct: 198 LQELLGIEKSLEAIR 212
>At5g38560 [T] KOG1187 Serine/threonine protein kinase
Length = 681
Score = 32.0 bits (71), Expect = 0.92
Identities = 19/62 (30%), Positives = 28/62 (44%)
Query: 29 PPKYTLATVRSFPSLEPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSSNPPGRSD 88
PP T A+ S +P T AP PT + P + + N+ S+ PG+S+
Sbjct: 173 PPPATSASPPSSNPTDPSTLAPPPTPLPVVPREKPIAKPTGPASNNGNNTLPSSSPGKSE 232
Query: 89 VG 90
VG
Sbjct: 233 VG 234
>At1g26170 [UY] KOG2274 Predicted importin 9
Length = 931
Score = 31.2 bits (69), Expect = 1.6
Identities = 30/114 (26%), Positives = 50/114 (43%), Gaps = 22/114 (19%)
Query: 96 ILPQKGSGMARAGDANSPIRHNGG---RALARTAPNDYT-TALPKKVYSLAFN------- 144
+L + S A+ SPI+ NGG R+ AR+AP +T LP K+ +L +
Sbjct: 761 LLSTRHSEFAKVNVPGSPIQSNGGITTRSKARSAPEQWTIIPLPMKILALLADTLIEIQE 820
Query: 145 NALSHQYRNGNVFVIGSGD-----------GISETSQADLNELEILPIGEGSED 187
LS++ + + GD G S+ S+ ++LE + E +D
Sbjct: 821 QVLSNEDEDSEWEEVHEGDAKAEKDLLRSAGTSQFSKPTYDQLEAMARFENQDD 874
>CE26551 [I] KOG2624 Triglyceride lipase-cholesterol esterase
Length = 684
Score = 30.8 bits (68), Expect = 2.0
Identities = 14/40 (35%), Positives = 21/40 (52%)
Query: 21 KPLPNASLPPKYTLATVRSFPSLEPLTFAPVPTSVFDAPL 60
KP+ N+ P T+ T+ + P+L P TF +P PL
Sbjct: 226 KPIQNSMFPTFPTMPTLPTLPTLAPFTFPTLPPPTTMKPL 265
>7290247 [R] KOG3656 FOG: 7 transmembrane receptor
Length = 634
Score = 30.4 bits (67), Expect = 2.7
Identities = 44/162 (27%), Positives = 56/162 (34%), Gaps = 18/162 (11%)
Query: 19 SNKPLPNASLPPKYTLATVRSFPSLEPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRV 78
SN P PN + PP PS+ LT P ++ LR L Y N
Sbjct: 466 SNLPQPNVTPPP----------PSV--LTATPNGSNSNSLTLRLPLKKNNHCYTNSGFNS 513
Query: 79 GSSNPPGRSDVGYSRRKI----LPQKGSGM-ARAGDANSPIRHN-GGRALARTAPNDYTT 132
+ +P +G S I + GSG+ N RHN GGR A A D+
Sbjct: 514 STPSPSSGLGIGISNSSIYRPGVGSLGSGLRLDPSHNNGGGRHNTGGRGAAAHASGDFCA 573
Query: 133 ALPKKVYSLAFNNALSHQYRNGNVFVIGSGDGISETSQADLN 174
+L + H N N SG G T D N
Sbjct: 574 DNAGTSPTLIPTASAVHGLINNNNQRRSSGSGEQCTKAGDAN 615
>Hs6005701 [IR] KOG0059 Lipid exporter ABCA1 and related proteins ABC
superfamily
Length = 1581
Score = 29.6 bits (65), Expect = 4.6
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 9/54 (16%)
Query: 205 KLLFVINEPRPGLFSFT---------ERFQDKVNIVQKEFVDVNDLLKAHRIFI 249
K +F+++EP GL F+ ER D+V + +F+D D+L ++F+
Sbjct: 595 KEIFLLDEPTAGLDPFSRHQVWNLLKERKTDRVILFSTQFMDEADILADRKVFL 648
>At1g05910 [O] KOG0732 AAA+-type ATPase containing the bromodomain
Length = 1069
Score = 29.6 bits (65), Expect = 4.6
Identities = 18/55 (32%), Positives = 28/55 (50%)
Query: 127 PNDYTTALPKKVYSLAFNNALSHQYRNGNVFVIGSGDGISETSQADLNELEILPI 181
P+ T++ K+ L F +A+ YR + + G G G+ A L+ELE PI
Sbjct: 547 PSSATSSELTKLSILTFGSAIPLVYRPRLLLLGGEGVGLDHLGPAILHELEKFPI 601
>7301301 [U] KOG0864 Ran-binding protein RANBP1 and related RanBD domain
proteins
Length = 2659
Score = 29.3 bits (64), Expect = 5.9
Identities = 27/91 (29%), Positives = 39/91 (42%), Gaps = 5/91 (5%)
Query: 99 QKGSGMARAGDANSPIRHNGGRALARTAPNDYTT--ALPKKVYSLAFNNALSHQYRN--G 154
QK G A++ S + G + PNDY T + K SL+ NN+ + +
Sbjct: 2137 QKNDGAAKSRGGESEVLDVGKTSSPLAKPNDYQTPATILFKGSSLSRNNSSASEASKTPS 2196
Query: 155 NVFVIGSGDGISETSQA-DLNELEILPIGEG 184
+ F+ GS D A L L+ L GEG
Sbjct: 2197 SAFIFGSTDKSEPGKDAGPLANLQKLASGEG 2227
>CE00373 [O] KOG2177 Predicted E3 ubiquitin ligase
Length = 851
Score = 28.9 bits (63), Expect = 7.8
Identities = 28/106 (26%), Positives = 42/106 (39%), Gaps = 19/106 (17%)
Query: 46 LTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGS-----------SNPPGRSDVGYSRR 94
L F P + V P + L A ++ N + V +N P +D
Sbjct: 392 LGFNPDTSGVLMTPCEIEYLGAAGLFNNSASTVSQLLGSVHGGSPINNAPAANDF----- 446
Query: 95 KILPQKGSGMARAGDANSPIRHNGGRALARTAPNDYTTALPKKVYS 140
++PQ +G+A G A S I LAR+ P+ +LP YS
Sbjct: 447 -LMPQ--AGLAPIGRAQSRIIPIEQNQLARSPPHHIAGSLPMNAYS 489
>At2g04190 [DR] KOG1987 Speckle-type POZ protein SPOP and related proteins
with TRAF MATH and BTB/POZ domains
Length = 412
Score = 28.9 bits (63), Expect = 7.8
Identities = 16/54 (29%), Positives = 27/54 (49%), Gaps = 3/54 (5%)
Query: 29 PPKYTLATVRSFPSLEPLTFAPVPTSVFDAPLRRDLLWRAVVYENDSKRVGSSN 82
PP + + T+ +F + P +SVF+A WR ++Y N ++ G SN
Sbjct: 119 PPSHRILTITNFSEIIGRE-EPYESSVFEAYFEHK--WRLILYVNGNQNDGGSN 169
>At1g64030 [V] KOG2392 Serpin
Length = 385
Score = 28.9 bits (63), Expect = 7.8
Identities = 26/113 (23%), Positives = 45/113 (39%), Gaps = 19/113 (16%)
Query: 109 DANSPIRHNGGRALARTAPNDYTTALPKKVY--SLAFNNA----LSHQYRNGNVFVIGSG 162
+ NS + H+ + P+ T+L K+Y +L+F A Y N F + +G
Sbjct: 138 EVNSWVEHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYLVNG 197
Query: 163 DGISETSQADLNELEI----------LPIGEGSEDGDIVFEKFLKHYELQNKK 205
+S + + LP GS+D + KF ++ L +KK
Sbjct: 198 TSVSVPFMSSYENQYVRAYDGFKVLRLPYQRGSDDTN---RKFSMYFYLPDKK 247
>7298249 [A] KOG0948 Nuclear exosomal RNA helicase MTR4 DEAD-box superfamily
Length = 1055
Score = 28.9 bits (63), Expect = 7.8
Identities = 20/65 (30%), Positives = 32/65 (48%), Gaps = 11/65 (16%)
Query: 192 FEKFLKHYELQNKKLLFVINEPRPGLFSFTERFQDKVNIVQKEFVDVNDLLKAHRIFIEL 251
FEK L+ + L P L R+QDKV + QK+ D+ LKA R +++
Sbjct: 813 FEKRLEEHPLHKS----------PELERIHRRYQDKVTL-QKQLQDLKAELKAARSLLQM 861
Query: 252 EALEY 256
+ L++
Sbjct: 862 DELKH 866
>7297532 [R] KOG0231 Junctional membrane complex protein Junctophilin and
related MORN repeat proteins
Length = 975
Score = 28.9 bits (63), Expect = 7.8
Identities = 17/51 (33%), Positives = 24/51 (46%), Gaps = 1/51 (1%)
Query: 79 GSSNPPGRSDVGYSRRKILPQKGSGMA-RAGDANSPIRHNGGRALARTAPN 128
G+S P GR G R PQ G+G + G+ + P + GGR P+
Sbjct: 830 GTSTPDGRPRAGSVFRGSTPQPGAGTSTMTGNGSLPSGNGGGRLSRAGTPS 880
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,122,583
Number of Sequences: 60738
Number of extensions: 806705
Number of successful extensions: 1843
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1830
Number of HSP's gapped (non-prelim): 22
length of query: 263
length of database: 30,389,216
effective HSP length: 104
effective length of query: 159
effective length of database: 24,072,464
effective search space: 3827521776
effective search space used: 3827521776
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)