ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0571 good J KOG2553 Translation, ribosomal structure and biogenesis Pseudouridylate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0571 201175 199517 -553
(553 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL212c [J] KOG2553 Pseudouridylate synthase 667 0.0
SPCC126.03 [J] KOG2553 Pseudouridylate synthase 367 e-101
YGL063w [J] KOG2553 Pseudouridylate synthase 280 5e-75
SPBC887.11 [J] KOG2553 Pseudouridylate synthase 220 5e-57
SPCC16C4.06c [J] KOG2553 Pseudouridylate synthase 196 1e-49
At1g20370 [J] KOG2553 Pseudouridylate synthase 196 1e-49
At1g76120 [J] KOG2553 Pseudouridylate synthase 184 4e-46
7300636 [J] KOG2553 Pseudouridylate synthase 158 2e-38
ECU04g0890 [J] KOG2553 Pseudouridylate synthase 151 2e-36
Hs13376820 [J] KOG2553 Pseudouridylate synthase 129 1e-29
At2g30320 [J] KOG2553 Pseudouridylate synthase 120 5e-27
CE20158 [J] KOG2553 Pseudouridylate synthase 96 2e-19
SPAC25B8.05 [J] KOG2554 Pseudouridylate synthase 71 5e-12
Hs13775234 [J] KOG2554 Pseudouridylate synthase 69 2e-11
YFL001w [J] KOG2554 Pseudouridylate synthase 50 7e-06
At3g06950 [AJ] KOG4393 Predicted pseudouridylate synthase 50 9e-06
>YPL212c [J] KOG2553 Pseudouridylate synthase
Length = 544
Score = 667 bits (1722), Expect = 0.0
Identities = 338/547 (61%), Positives = 401/547 (72%), Gaps = 55/547 (10%)
Query: 4 EQNLRPVYDDDQPGEDTYKRGAIYKQTRSRKADYEDGENSEKRQKPNDTDNGPTAVVTDL 63
E+NLRP YDD Q ED YKRGA K T++RKAD++D ++ ++K ND
Sbjct: 3 EENLRPAYDD-QVNEDVYKRGAQSKLTKARKADFDDEKD---KKKDND------------ 46
Query: 64 SNEKKETRSKDKDESVALAVDADGNPIPQEVRLPKRKVAVMIGYCGTGYHGMQYNPPNDT 123
+ DK +D +GNP+P+E RLPKRKVAVM+GYCGTGYHGMQYNPPN T
Sbjct: 47 -------KHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPT 99
Query: 124 IEKELFEAFVRAGAISKANSTDLKKNGFQRAARTDKGVHAGGNVISLKLIIEDPEVKEKI 183
IE LF+AFV AGAISK NS DLKKNGF RAARTDKGVHAGGN+ISLK+IIEDP++K+KI
Sbjct: 100 IESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKI 159
Query: 184 NNELPDQIRVWDISRVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEASKKEI 243
N +LP+ IRVWDI RVNKAFDCRKMCSSRWYEYLLPTYSLIGPKP + LY +IE SK E+
Sbjct: 160 NEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTEL 219
Query: 244 PNVIQDDVESAQFWEAFSTEAESKFTKEELEEIAAFVMP-KDSFDENNETYQKSKAFKKL 302
P V+ +D+ES +FWE F +A KF+ EE+E I A+V P +D FD N E YQK K +K+L
Sbjct: 220 PGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKYKQL 279
Query: 303 EAEHKRAYRISKERLDRFRSALKQYEGTFNFHNFTLGKDFNDPSAKRFMKQITVSEPFVI 362
E H+R YRIS +L +FR++ QY G NFHNFTLGKDF +PSA RFMK I VS+PFVI
Sbjct: 280 ENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVSDPFVI 339
Query: 363 GEAKTEWVSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQEKINIPKAPALG 422
G+A+TEW+SIKIHGQSFMLHQIRKM+SMATLITRC P+ERI QAYGQ+KINIPKAPALG
Sbjct: 340 GDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINIPKAPALG 399
Query: 423 LLLEAPVYDGYNTRLQEFGYDPIDFSKYQSEMDTFKMKHIYDKIYLEEVNENVFNAFFSY 482
LLLEAPV++GYN RL++FGY IDFSKYQ E+D FKMKHIYDKIY EEV+ENVFNAFFSY
Sbjct: 400 LLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDENVFNAFFSY 459
Query: 483 IDTFNPSYNIXXXXXXXXXXXXXXXXXAVELVTEDLEQKAPSDPTPSDEKGKKPQRPIFD 542
ID+FN + +D+ G Q+ IF+
Sbjct: 460 IDSFN-------------------------------KVTGAQGEETADKSGPAVQKSIFE 488
Query: 543 FLTARGI 549
FLTA+GI
Sbjct: 489 FLTAKGI 495
>SPCC126.03 [J] KOG2553 Pseudouridylate synthase
Length = 534
Score = 367 bits (941), Expect = e-101
Identities = 211/518 (40%), Positives = 289/518 (55%), Gaps = 91/518 (17%)
Query: 31 RSRKADYEDGENSE-KRQKPNDTDNGPTAVVTDLSNEKKETRSKDKDESVALAVDADGNP 89
R K + +G+ E KR +PN NG + EKK
Sbjct: 3 RGGKRTWYNGDRREAKRNRPNSIYNGEGRPENLVVGEKK--------------------- 41
Query: 90 IPQEVRLPKRKVAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKN 149
PKRKVA ++GYCG+GYHGMQ NPP+ TIE +LF+AFV+AGA+S N+ D KK
Sbjct: 42 -------PKRKVACLVGYCGSGYHGMQLNPPSKTIEGDLFDAFVKAGAVSSYNADDPKKV 94
Query: 150 GFQRAARTDKGVHAGGNVISLKLIIEDPEVKEKINNELPDQIRVWDISRVNKAFDCRKMC 209
RAARTDKGVHA GNVISLKLI+ED ++ EK+N LP IR+WD+ R +F+ R C
Sbjct: 95 ALARAARTDKGVHAAGNVISLKLIMEDEKLIEKVNEHLPPSIRLWDVIRTINSFNPRTYC 154
Query: 210 SSRWYEYLLPTYSLIGPKPSTYLYNEI--------EASKKEIPNVIQDDV---ESAQFWE 258
SR YEY++PTY+ + PKPS+ L N I E KE N + + E +FW+
Sbjct: 155 ESRIYEYMVPTYAFVPPKPSSILGNCIMKNSPMPAEPINKENINQLSRSLFYEEGKEFWD 214
Query: 259 AFSTEAE--------------------------------------SKFTKEELEE----- 275
+ A+ S T +++
Sbjct: 215 DYDIAAKEILSLYEQDPEGFVNPYSKRGAAALANSENNKGSEAGVSAKTNPDMDSDSSAI 274
Query: 276 IAAFVMPKDSFDEN-----NETYQKSKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGT 330
+ F+ P DE+ + +Y+ +A K +E + YRIS +RL R L QY G
Sbjct: 275 VNEFLKPDSVEDESAGSKIDPSYRLERALKHIEVLKLKNYRISADRLSVIRETLNQYVGV 334
Query: 331 FNFHNFTLGKDFNDPSAKRFMKQITVSEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISM 390
NFHNFT+G+ F+ ++ R ++ T S+PF+IG+ TEW+S K+HGQSFMLHQIRKMI++
Sbjct: 335 HNFHNFTVGQAFHQKNSNRVIRSFTASDPFMIGD--TEWISCKVHGQSFMLHQIRKMIAL 392
Query: 391 ATLITRCFSPLERIRQAYGQEKINIPKAPALGLLLEAPVYDGYNT-RLQEFGYDPIDFSK 449
A L+ R P+ERI+ A+ + KINIPK P GLLLE+P + GYN + E DPIDF+K
Sbjct: 393 AILVVRTGCPVERIQDAFKKTKINIPKGPGFGLLLESPFFKGYNEHKAPENNRDPIDFTK 452
Query: 450 YQSEMDTFKMKHIYDKIYLEEVNENVFNAFFSYIDTFN 487
Y+ ++ FK HIYDKI+LEE + VF+ F S+ID++N
Sbjct: 453 YEQKITAFKHAHIYDKIFLEEARKQVFHCFLSFIDSYN 490
>YGL063w [J] KOG2553 Pseudouridylate synthase
Length = 370
Score = 280 bits (715), Expect = 5e-75
Identities = 159/380 (41%), Positives = 225/380 (58%), Gaps = 25/380 (6%)
Query: 103 VMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAARTDKGVH 162
+++GYCG+GY+GMQYNPP+ TIE E+ GAIS+ NS KKN F AARTDKGVH
Sbjct: 1 MLLGYCGSGYYGMQYNPPHKTIEGEILTKLFDVGAISEENSLAPKKNSFMAAARTDKGVH 60
Query: 163 AGGNVISLKLIIEDPEVKEKINNELPDQIRVWDISRVNKAFDCRKMCSSRWYEYLLPTYS 222
A N++SLK+ + + V K+N LP +IRVW I VNK F+ R C SRWY+YL+P +
Sbjct: 61 AMLNLLSLKITLREDTV-AKLNAALPPEIRVWGIQPVNKKFNARSACDSRWYQYLIPEFI 119
Query: 223 LIGPKPSTYLYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAESKFTKEELEEIAAFVMP 282
LIGP S+ L+ + S +E S + W+ F + +F+ +EL +
Sbjct: 120 LIGPPRSSLLHRNVGGSYRE--------DGSQEVWDTFLEQTRGRFSGDELCRL------ 165
Query: 283 KDSFDENNETYQKSKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNFHNFTLGKDF 342
+D+ + +E+ + + L + Y +S +LD F +A+++Y GT NFHNFT GK +
Sbjct: 166 QDTAQKLSESDPLVQDYVGLLSGTLSGYCLSPSKLDAFEAAMQEYVGTHNFHNFTTGKLW 225
Query: 343 NDPSAKRFMKQITVSEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISMATLITRCFSPLE 402
DPSA+R +K++ VS +A W+ ++IHGQSFMLHQIR+M+++A L RC P
Sbjct: 226 GDPSAQRHIKKVVVS------QASPGWICVRIHGQSFMLHQIRRMVALAVLAARCQLPPN 279
Query: 403 RIRQAY--GQEKINIPKAPALGLLLEAPVYDGYNTRLQEFGYDPIDFSKYQSE-MDTFKM 459
+R + G K IP+APA GLLLE PV+DGYNT+L+ Y I E M F+
Sbjct: 280 IVRNYFNAGPRKY-IPRAPAQGLLLEGPVFDGYNTKLRNLLYCEIRPDDITLERMCRFRE 338
Query: 460 KHIYDKIYLEEVNENVFNAF 479
+ I I EE +VF F
Sbjct: 339 RQICTAIAHEETQRHVFCHF 358
>SPBC887.11 [J] KOG2553 Pseudouridylate synthase
Length = 451
Score = 220 bits (560), Expect = 5e-57
Identities = 148/448 (33%), Positives = 236/448 (52%), Gaps = 46/448 (10%)
Query: 70 TRSKDKDESVALAVDADGNPIPQEVRLP------KRKVAVMIGYCGTGYHGMQYNPPNDT 123
++ K++ E + A D + +P+ ++ K ++ +++GY G GYHG+Q N P T
Sbjct: 5 SKRKNQQEHIP-AEDLETPKLPKREKIEGTKESNKVRIIILLGYSGYGYHGIQINNPLKT 63
Query: 124 IEKELFEAFVRAGAISKANSTDLKKNGFQRAARTDKGVHAGGNVISLKLIIEDPE----V 179
IE ++ + G + K N+ D + RAARTDKGVH N+ISL L ++ P +
Sbjct: 64 IEGDVVAVLKKLGYL-KTNNIDAEHLCIARAARTDKGVHTLRNLISLNLFVDKPLDISLL 122
Query: 180 KEKINNELPDQIRVWDISRVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEAS 239
K ++N L QIRVW + K F+ R C SR YEYL+P+++L+ PKPS L+ +++ +
Sbjct: 123 KTELNEALCSQIRVWSVFPAPKYFNPRISCESRTYEYLIPSFALLPPKPSCPLFKKMQKN 182
Query: 240 -KKEIPNVIQDDV-----ESAQFWEAFSTEAESKFTKEELEEIAAFVMPKDSFDE--NNE 291
+++ N ++ ++ + FW K ++E++E+ F KD+F
Sbjct: 183 LSRKLDNELERNLVYSMNDLISFWNTV------KLKQKEIQEM--FDTNKDAFTNPFKGM 234
Query: 292 TYQK--------------SKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNFHNFT 337
Y+K KA K+ E YRI ++RL + LK+YEG NFHNFT
Sbjct: 235 FYEKPIPAGIVIPPQAKLKKALKQAEYYCYMNYRIKEDRLKVLQQLLKKYEGRHNFHNFT 294
Query: 338 LGKDFNDPSAKRFMKQITVSEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISMATLITRC 397
+ D PS RF++ +T PFV EW+ + I G SFML+QIRKM++ +I R
Sbjct: 295 VTDDSTSPSNYRFIESVTCGTPFVY--ENWEWIPVTIKGNSFMLNQIRKMMAHVLMIIRS 352
Query: 398 FSPLERIRQAYGQE-KINIPKAPALGLLLEAPVYDGYNTRLQEFGYDPIDFSKYQSEMDT 456
+P I +A+ +NI K+P LLL+ + YN + + G + I F ++ ++ +
Sbjct: 353 CAPTGLIDKAFDPNITMNISKSPGHVLLLKDIKFSSYNDSVTD-GLEKIQFDCFEEDILS 411
Query: 457 FKMKHIYDKIYLEEVNENVFNAFFSYID 484
K+K IY I E E +F +F SYID
Sbjct: 412 LKIKTIYPDIIKLEQKEKLFFSFLSYID 439
>SPCC16C4.06c [J] KOG2553 Pseudouridylate synthase
Length = 413
Score = 196 bits (497), Expect = 1e-49
Identities = 129/391 (32%), Positives = 194/391 (48%), Gaps = 46/391 (11%)
Query: 94 VRLPKRKVAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQR 153
V+ KR V ++ Y GTG+ G+QYN TI+ LF+AF R GA+ + N+ KK
Sbjct: 32 VKPRKRMVYCVVSYRGTGFAGLQYNANVKTIQDTLFQAFARVGAVVQVNADSPKKIRMCS 91
Query: 154 AARTDKGVHAGGNVISLKLIIED--PEVKEKINNELPDQIRVWDISRVNKAFDCRKMCSS 211
AARTDKGVHA NV+ LK++ P V +N+ LP IRVW ++R +F +C S
Sbjct: 92 AARTDKGVHAIVNVLGLKVLDNQPLPHVVNLVNDILPPCIRVWKMARTFNSFSPHTVCDS 151
Query: 212 RWYEYLLPTYSLIGPKPSTY-LYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAESKFTK 270
R YEY LP SL+ P+P T Y +AS+K P
Sbjct: 152 RVYEYWLPVSSLLTPRPCTLEAYVIAKASEKAFP------------------------IN 187
Query: 271 EELEEIAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGT 330
E L +A N + Q A E +R+SK +LD + A + GT
Sbjct: 188 ETLSHLA------------NLSKQDCVANSLSE-----PFRLSKPKLDFLKHACTMFRGT 230
Query: 331 FNFHNFTLGKDFNDPSAKRFMKQITVSEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISM 390
FH++T K F+D S++RF+ + V + I + +WV + HGQSFM HQIRKM+ +
Sbjct: 231 HRFHSYTTEKGFSDASSRRFLLDVRV-DNLHIDKLNRQWVKLIFHGQSFMKHQIRKMVGI 289
Query: 391 ATLITRCFSPLERIRQAYGQE-KINIPKAPALGLLLEAPVYDGYNTRLQEFGYDPIDFSK 449
+TR + + + +I IP+APA LLL P++ +N + F ++P+++S
Sbjct: 290 LIHLTRTGWNAQVLLNTFNNSYRIRIPRAPAEFLLLNQPIFQAFNKKCSRFDHEPVEWSC 349
Query: 450 YQSEMDTFKMKHIYDKIYLEEVNENVFNAFF 480
Q +D F + ++ ++ F +FF
Sbjct: 350 AQKGIDQFAHSFLRTPMFDCFISSESFQSFF 380
>At1g20370 [J] KOG2553 Pseudouridylate synthase
Length = 549
Score = 196 bits (497), Expect = 1e-49
Identities = 146/471 (30%), Positives = 234/471 (48%), Gaps = 56/471 (11%)
Query: 45 KRQKPNDTDNGPTAVVTDLSNEKKETR-SKDKDESVALAVDADGNPIPQEVRLPK---RK 100
+ Q+P D + P V D + K+ + S E+ + AV G+ + + R PK RK
Sbjct: 2 ENQEP-DIEKLPRDDVPDREPDHKKLKISTTGQETDSSAVTTGGSNVAER-RAPKYRRRK 59
Query: 101 VAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAARTDKG 160
VA++ +CG GY GMQ NP TIE EL EA AGA+ A+ + + R+ARTDKG
Sbjct: 60 VAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKG 119
Query: 161 VHAGGNVISLKLIIEDPEVKEKINNELPDQIRVWDISRVNKAFDCRKMCSSRWYEYLLPT 220
V A G V+S + ++ P E++N++LPDQIRV+ RV +F +K C R Y YL+P
Sbjct: 120 VSAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPV 179
Query: 221 YSL---------IGPKPSTYLYNE-IEASKK--EIP---------------NVIQDDVES 253
++L + Y Y + +E S+K +IP IQ D+ S
Sbjct: 180 FALDPCVHGEAEMVRTDLGYEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISS 239
Query: 254 A-----------QFWEAFSTEAESKFTKEELE--EIAAFVMPKDSFDENNE----TYQKS 296
+ + + AE+ E L+ +++A + +E+N
Sbjct: 240 STCDAIGADVKCETLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESNTLAKVEMNNG 299
Query: 297 KAFKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNFHNFTLGKDFNDPSAKRFMKQITV 356
+ ++E K Y ++ +RF L Y G+ NFHNFT DP+A R++ ++
Sbjct: 300 EGEDMTKSESKFCY--GEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYI--LSF 355
Query: 357 SEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQE-KINI 415
+ VI ++V ++ GQSFMLHQIRKMI +A I R ++P I ++ ++ +IN+
Sbjct: 356 NANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINV 415
Query: 416 PKAPALGLLLEAPVYDGYNTRLQEFGYDPIDFSKYQSEMDTFKMKHIYDKI 466
P AP +GL L+ + YN R + ++ + +Y+ + FK K++Y I
Sbjct: 416 PMAPEVGLYLDECFFTSYNKRFKG-SHEEVSMEEYKEVAEEFKWKYVYSHI 465
>At1g76120 [J] KOG2553 Pseudouridylate synthase
Length = 463
Score = 184 bits (466), Expect = 4e-46
Identities = 128/417 (30%), Positives = 201/417 (47%), Gaps = 37/417 (8%)
Query: 76 DESVALAVDADGNPIPQEVRLPKRKVAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRA 135
DE + L+ D G PQ KVA++ +CG GY GMQ NP TIE EL EA A
Sbjct: 7 DEEIDLSCDG-GVTEPQ-------KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFHA 58
Query: 136 GAISKANSTDLKKNGFQRAARTDKGVHAGGNVISLKLIIEDPEVKEKINNELPDQIRVWD 195
GA+ ++ K F R+ARTDKGV A G V+S + I++ ++N+ LP+QIR++
Sbjct: 59 GAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIFG 118
Query: 196 ISRVNKAFDCRKMCSSRWYEYLLPTYSL--------------IGPKPSTYLYNEIEASKK 241
V +F +K C R Y YLLP ++L +GP E +
Sbjct: 119 YKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGR 178
Query: 242 EIPNVI-------QDDVESAQFWEAFSTEAESKFTKE-ELEEIAAFVMPKDSFDENNETY 293
+IP + +S F S+ S + ++E +++ + S D
Sbjct: 179 KIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRI 238
Query: 294 QKSKA---FKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNFHNFTLGKDFNDPSAKRF 350
Q+ E + K + ++ +RF L Y G++NFHNFT +DP+A R
Sbjct: 239 QEDSCKLNTNSSETKVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQ 298
Query: 351 MKQITVSEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQ 410
+ T + VI +++ ++ G+SFMLHQIRKM+ +A I R + I+ A+ +
Sbjct: 299 IISFTANT--VINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSK 356
Query: 411 E-KINIPKAPALGLLLEAPVYDGYNTRLQEFGYDPIDFSKYQSEMDTFKMKHIYDKI 466
+ I +P AP +GL L+ + YN ++ ++ + Y+ E + FK+KHIY I
Sbjct: 357 DVNITVPMAPEVGLYLDECFFTSYNRNFED-SHEEVSMEAYKEEAEAFKLKHIYSHI 412
>7300636 [J] KOG2553 Pseudouridylate synthase
Length = 410
Score = 158 bits (400), Expect = 2e-38
Identities = 117/410 (28%), Positives = 191/410 (46%), Gaps = 82/410 (20%)
Query: 67 KKETRSKDKDESVALAVDADGNPIPQEVRLPKRKVAVMIGYCGTGYHGMQYNPPNDTIEK 126
KK K++DE A A P R+ ++K A+++ YCG Y+GMQ NP TIE+
Sbjct: 39 KKWVDWKEQDEKDA-ANGVKRAPFDPADRIKRKKSAILLSYCGANYYGMQRNPGMQTIEE 97
Query: 127 ELFEAFVRAGAISKANSTDLKKNGFQRAARTDKGVHAGGNVISLKLIIEDPEV--KEKIN 184
ELF+A ++ I++ + ++ + FQRAARTDKGV A V S+KL PE E N
Sbjct: 98 ELFKAMLKHKWITEDSFEQIQISCFQRAARTDKGVSAARQVCSVKL----PEELDLEAFN 153
Query: 185 NELPDQIRVWDISRVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEASKKEIP 244
+LP QIR++ + RV K F+ + C++R Y Y LPT + P +++ + + P
Sbjct: 154 ADLPQQIRLFGVERVTKGFNAKDQCNARTYTYTLPTVAF---APFEEKVDDVHDTFRISP 210
Query: 245 NVIQDDVESAQFWEAFSTEAESKFTKEELEEIAAFVMPKDSFDENNETYQKSKAFKKLEA 304
++Q E+ + +E +N + K+F L+
Sbjct: 211 ELLQKVKETLKLYEG---------------------------TKNFHNFTSKKSF--LDP 241
Query: 305 EHKRAYRISKERLDRFRSALKQYEGTFNFHNFTLGKDFNDPSAKRFMKQITVSEPFVIGE 364
KR +FT + F P F
Sbjct: 242 SSKRF-----------------------IMSFTSSEPFRSPQDIEF-------------- 264
Query: 365 AKTEWVSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQEKINIPKAPALGLL 424
V++K+ GQSFMLHQIRKM+ +A I R + + +A +E++++P AP LGL+
Sbjct: 265 -----VTLKVKGQSFMLHQIRKMVGLAIAIVRGNTTAATLERALTEERLDLPMAPGLGLV 319
Query: 425 LEAPVYDGYNTRLQEFG-YDPIDFSKYQSEMDTFKMKHIYDKIYLEEVNE 473
L+ Y+ YN R + G ++P+ + ++++ F + I+ +IY E +
Sbjct: 320 LDTVHYERYNDRYGKDGIHNPLTWQAQEAQVQEFIEREIFSQIYKTEAEQ 369
>ECU04g0890 [J] KOG2553 Pseudouridylate synthase
Length = 345
Score = 151 bits (382), Expect = 2e-36
Identities = 117/392 (29%), Positives = 192/392 (48%), Gaps = 64/392 (16%)
Query: 98 KRKVAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAART 157
K +V +++GY G+ YHG+Q+N DT+EK++ E +R AIS+ N+ D +K + ++RT
Sbjct: 2 KIRVGLLVGYDGSRYHGLQFNKDLDTVEKKIVECLLRMDAISERNAGDPRKIHIKSSSRT 61
Query: 158 DKGVHAGGNVISLKLII-EDPEVKEKINNELPDQ-IRVWDISRVNKAFDCRKMCSSRWYE 215
DKGVHA N++S+K+ + PE+ + L + I ++DI R+ K+ K SR YE
Sbjct: 62 DKGVHAALNLVSVKIELGVSPELVSGLRELLSKEGIHLYDIVRLTKSIVPSKQAISRTYE 121
Query: 216 YLLPTYSLIGPKPSTYLYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAESKFTKEELEE 275
Y++PTY L AE F++E
Sbjct: 122 YIVPTYFL----------------------------------------AEGDFSRE---- 137
Query: 276 IAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNFHN 335
V DE + + + + + Y S++ F + L++Y GT +FHN
Sbjct: 138 ----VEVLSQGDEECRGDRIGRVYPESMIDSVVGYTSSEDDTRVFSTILQKYVGTKDFHN 193
Query: 336 FTLGKDFNDPSAKRFMKQITVSEPFVIGEAKTEWVSIKIHGQSFMLHQIRKMISMATLIT 395
FT K N+ + R++K I VS+ + + E+V I I GQSF+LHQIRKM+ A L+
Sbjct: 194 FT--KLNNEKGSTRYIKSIIVSDTYT--DNGVEYVKISITGQSFLLHQIRKMVLFAVLLC 249
Query: 396 RCFSPL--ERIRQAYGQEKINIPKAPALGLLLEAPVYDGYNTRLQEFGYDPIDFSKYQSE 453
R + + + I++PK P+ LLL+ P++ + R + + I S S+
Sbjct: 250 RYSRNAVDSKFDMVFSRHNIHVPKGPSEYLLLDKPIF--HKLRRGDGTTEHIGVS--DSD 305
Query: 454 MDTFKMKHIYDKIYLEEVNENVFNAFFSYIDT 485
+ +K IY KI+ E N +FF+ +D+
Sbjct: 306 REEYKRSTIYPKIH-ERKN---LTSFFACMDS 333
>Hs13376820 [J] KOG2553 Pseudouridylate synthase
Length = 399
Score = 129 bits (324), Expect = 1e-29
Identities = 68/179 (37%), Positives = 107/179 (58%), Gaps = 3/179 (1%)
Query: 310 YRISKERLDRFRSALKQYEGTFNFHNFTLGKDFNDPSAKRFMKQITVSEPFVIGEAKTEW 369
YR+S E L + L Y+GT NFHNFT K DPSA R++ ++ EPFV E+
Sbjct: 194 YRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFV--REGLEF 251
Query: 370 VSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQEKINIPKAPALGLLLEAPV 429
I++ GQSFM+HQIRKM+ + I + ++P + +++G EK+++PKAP LGL+LE
Sbjct: 252 AVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVH 311
Query: 430 YDGYNTRLQEFG-YDPIDFSKYQSEMDTFKMKHIYDKIYLEEVNENVFNAFFSYIDTFN 487
++ YN R G ++P+D+++ + ++ FK +HIY I E +E + S + N
Sbjct: 312 FEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHN 370
Score = 118 bits (295), Expect = 3e-26
Identities = 60/129 (46%), Positives = 84/129 (64%), Gaps = 4/129 (3%)
Query: 97 PKRKVAVMIGYCGTGYHGMQYNPPND---TIEKELFEAFVRAGAISKANSTDLKKNGFQR 153
PKRK+ +++ Y G GYHGMQ N + TIE +L A VR+G I + + D++K FQR
Sbjct: 54 PKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQR 113
Query: 154 AARTDKGVHAGGNVISLKLIIEDPEVKEKINNELPDQIRVWDISRVNKAFDCRKMCSSRW 213
ARTDKGV A G V+SLK+ + D ++ EKIN+ LP IR+ + RV F+ + C +R
Sbjct: 114 CARTDKGVSAAGQVVSLKVWLID-DILEKINSHLPSHIRILGLKRVTGGFNSKNRCDART 172
Query: 214 YEYLLPTYS 222
Y YLLPT++
Sbjct: 173 YCYLLPTFA 181
>At2g30320 [J] KOG2553 Pseudouridylate synthase
Length = 510
Score = 120 bits (301), Expect = 5e-27
Identities = 104/359 (28%), Positives = 171/359 (46%), Gaps = 39/359 (10%)
Query: 98 KRKVAVMIGYCGTGYHGMQY---NPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRA 154
K+KV + IGY GT Y G+Q +P TIE EL A +AG I +N DL K G+ R+
Sbjct: 62 KKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARS 121
Query: 155 ARTDKGVHAGGNVISLKLII------EDPE---VKEKINNELPDQIRVWDISRVNKAFDC 205
+RTDKGVH+ ISLK+ I +DP+ + + I+ LP+ IRV+ + N+ FD
Sbjct: 122 SRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDP 181
Query: 206 RKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAE 265
R+ C+ R Y YLLP ++G K +++ +EI+ + ++++ F +
Sbjct: 182 RRECTLRKYSYLLPV-DVLGIK-NSFTSDEIDYHITDFNEILKEFEGEYPF---HNYTQR 236
Query: 266 SKFTKEELEEIAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRI------SKERLDR 319
S++ ++ ++I N ++ K+ K E+E + I ++ +D
Sbjct: 237 SRYRRKSEQKIK---------QRNGRPPREPKSIKASESEFREENHIEIGEEEEEKEVDG 287
Query: 320 FRSALKQYEGTFNFHNFTLGKDFNDPSAKRFMKQITVSEPFVIGEAKTE------WVSIK 373
+ N ++ K +P + + F K E +V I
Sbjct: 288 ESDEHVVTPDSDNSQVYSRAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEIS 347
Query: 374 IHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQ-EKINIPKAPALGLLLEAPVYD 431
I G+SFMLHQIRKMI A + R P + IR + + +I +P AP+ L+L ++
Sbjct: 348 IWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFE 406
>CE20158 [J] KOG2553 Pseudouridylate synthase
Length = 415
Score = 95.5 bits (236), Expect = 2e-19
Identities = 57/163 (34%), Positives = 85/163 (51%), Gaps = 5/163 (3%)
Query: 279 FVMPKDSFDENNETYQ-KSKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNFHNFT 337
F P+ D +Y S F K ++R+S E L S L Y GT NF N+T
Sbjct: 140 FFHPQKQCDHRTYSYTCPSFVFAKPTELTNSSFRLSPETLAEVNSILSIYLGTHNFFNYT 199
Query: 338 LGKDFNDPSAKRFMKQITVSEPFVIGE----AKTEWVSIKIHGQSFMLHQIRKMISMATL 393
+ ++D S+ R++ EPF+ + E+V I I GQSF+LHQIRKM+ M
Sbjct: 200 AKRAYDDMSSNRYIISFECKEPFLFRDDFRKEDVEFVQIVIKGQSFVLHQIRKMVGMVIT 259
Query: 394 ITRCFSPLERIRQAYGQEKINIPKAPALGLLLEAPVYDGYNTR 436
+ R I++++ ++++IP AP LGLLLE YD Y+ +
Sbjct: 260 VIRELHLKSSIQRSFEGQRMDIPMAPGLGLLLERTHYDSYDRK 302
Score = 82.8 bits (203), Expect = 1e-15
Identities = 50/144 (34%), Positives = 74/144 (50%), Gaps = 4/144 (2%)
Query: 91 PQEVRLPKRKVAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNG 150
P++ R+ + K A+++GY G Y+GMQ TIE +L EA AG I+ A
Sbjct: 25 PKKPRVKQAKYAMLLGYQGKKYYGMQLQKDLPTIESKLLEAMETAGWITTAQKEKPFDFF 84
Query: 151 FQRAARTDKGVHAGGNVISLKLIIEDPEVKEK----INNELPDQIRVWDISRVNKAFDCR 206
FQRAARTD+ V A + ++L +D + + + +NN LP IRV+ + R F +
Sbjct: 85 FQRAARTDRAVSAARQMCGMQLPRDDAKYQAEGAGILNNILPADIRVFGMRRTTNFFHPQ 144
Query: 207 KMCSSRWYEYLLPTYSLIGPKPST 230
K C R Y Y P++ P T
Sbjct: 145 KQCDHRTYSYTCPSFVFAKPTELT 168
>SPAC25B8.05 [J] KOG2554 Pseudouridylate synthase
Length = 450
Score = 70.9 bits (172), Expect = 5e-12
Identities = 67/204 (32%), Positives = 87/204 (41%), Gaps = 25/204 (12%)
Query: 24 GAIYKQTRSRKADYEDGENSEKRQKPNDTDNGPTAVVTDLSNEKKETRSKDKDESVALAV 83
G + K T S +G+N + K N DNG LS E +
Sbjct: 23 GKLQKNTNSLPNKEIEGKNEVIKNK-NKLDNGNEIEPQHLSQELVLQHALQ--------- 72
Query: 84 DADGNPIPQEVR---LPKRK-VAVMIGYCGTGYHGMQYN---PPNDTIEKELFEAFVRAG 136
DA P + VR L K VA YCG Y+G+ Y P T+E ++FEA +RA
Sbjct: 73 DASSLPKLKPVRPFQLEKTVFVAFKFAYCGWNYNGLAYQLEPTPLPTVEGKVFEALLRAH 132
Query: 137 AISKANSTDLKKNGFQRAARTDKGVHAGGNVISLKLIIEDPE---VKEKINNELPDQIRV 193
I TD F R RTDKGV A G VISL + + + +N LP IR+
Sbjct: 133 LI-----TDPSSCSFSRCGRTDKGVSAMGQVISLNVRHSERRPIIYCDVLNRLLPADIRI 187
Query: 194 WDISRVNKAFDCRKMCSSRWYEYL 217
+ + FD R C R Y+YL
Sbjct: 188 TGYAFPPEGFDARFSCKQRHYKYL 211
>Hs13775234 [J] KOG2554 Pseudouridylate synthase
Length = 481
Score = 68.9 bits (167), Expect = 2e-11
Identities = 63/206 (30%), Positives = 87/206 (41%), Gaps = 37/206 (17%)
Query: 50 NDTDNGPTAV----VTDLSNEK---KETRSKDKDESVALAVDADGNPIPQEVRLP---KR 99
NDTD T V +L E K+ ++K+K++S A + +R
Sbjct: 4 NDTDRNQTEKLLKRVRELEQEVQRLKKEQAKNKEDSNIRENSAGAGKTKRAFDFSAHGRR 63
Query: 100 KVAVMIGYCGTGYHGM-QYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAARTD 158
VA+ I Y G GY G N+TIE++LFEA + + +++ + R RTD
Sbjct: 64 HVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRLVESRQTSN-----YHRCGRTD 118
Query: 159 KGVHAGGNVISLKL--------IIEDPEVKEK-------------INNELPDQIRVWDIS 197
KGV A G VISL L ED VKE+ +N LP IR+ +
Sbjct: 119 KGVSAFGQVISLDLRSQFPRGRDSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWA 178
Query: 198 RVNKAFDCRKMCSSRWYEYLLPTYSL 223
V +F R C R Y Y P L
Sbjct: 179 PVEPSFSARFSCLERTYRYFFPRADL 204
>YFL001w [J] KOG2554 Pseudouridylate synthase
Length = 442
Score = 50.4 bits (119), Expect = 7e-06
Identities = 42/137 (30%), Positives = 60/137 (43%), Gaps = 21/137 (15%)
Query: 99 RKVAVMIGYCGTGYHGMQYNP---PNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAA 155
R +A+ Y G Y+G+ P T+E + EA + + D K F R
Sbjct: 92 RFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYK---FSRCG 148
Query: 156 RTDKGVHAGGNVISLKL--IIEDPEVKEKINNE------------LPDQIRVWDIS-RVN 200
RTDKGV A VISL++ + D E ++ N+ LPD IR+ + R
Sbjct: 149 RTDKGVSAMNQVISLEVRSNLTDEEQRDPTNDSREIPYVHVLNQLLPDDIRISAVCLRPP 208
Query: 201 KAFDCRKMCSSRWYEYL 217
FD R C R Y+Y+
Sbjct: 209 PNFDARFSCVHRHYKYI 225
>At3g06950 [AJ] KOG4393 Predicted pseudouridylate synthase
Length = 298
Score = 50.1 bits (118), Expect = 9e-06
Identities = 50/187 (26%), Positives = 81/187 (42%), Gaps = 17/187 (9%)
Query: 100 KVAVMIGYCGTGYHGMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAARTDK 159
K ++I Y GT + G QY TI+ L +A ++ I++ +L+ G A RTD
Sbjct: 17 KWRLVIAYDGTRFAGWQYQESPPTIQSMLEKALIQ---ITELGRKELQLIG---AGRTDA 70
Query: 160 GVHAGGNVISLKLIIEDPEVKE---KINNELPDQIRVWDISRVNKAFDCRKMCSSRWYEY 216
GVHA G V + +N LP IRV ++S F R SS+ Y Y
Sbjct: 71 GVHAWGQVAHFVTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRY 130
Query: 217 LLPTYSLIGPKPSTYLYN---EIEASK-KEIPNVIQDDVESAQFWEAFSTEAESKFTKEE 272
+ + + P + Y+ ++ ASK +E N+ + + AF+ +
Sbjct: 131 QIYNDTFMDPFQRHWAYHCAYKLNASKMREAANLFVGKHD----FSAFANATREDGVPDP 186
Query: 273 LEEIAAF 279
L+ I+ F
Sbjct: 187 LKTISRF 193
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.133 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,683,120
Number of Sequences: 60738
Number of extensions: 1559195
Number of successful extensions: 4579
Number of sequences better than 1.0e-05: 16
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4511
Number of HSP's gapped (non-prelim): 29
length of query: 553
length of database: 30,389,216
effective HSP length: 111
effective length of query: 442
effective length of database: 23,647,298
effective search space: 10452105716
effective search space used: 10452105716
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)