ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0598 good R KOG1337 General function prediction only N-methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0598 208684 210465 594
(594 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL208w [R] KOG1337 N-methyltransferase 327 4e-89
YHL039w [R] KOG1337 N-methyltransferase 132 1e-30
SPBC1709.13c [R] KOG1337 N-methyltransferase 104 3e-22
SPAC3C7.09 [R] KOG1337 N-methyltransferase 63 1e-09
At3g07670 [R] KOG1337 N-methyltransferase 51 4e-06
>YPL208w [R] KOG1337 N-methyltransferase
Length = 583
Score = 327 bits (837), Expect = 4e-89
Identities = 197/599 (32%), Positives = 323/599 (53%), Gaps = 32/599 (5%)
Query: 2 DKLDQLLAFAKDNGVSLPQNVGFKNVEGKGICCIASDDIEQAVFQLPSHLIITKDLSNKH 61
D L LL + GV +P+ + F + KGI C+ DI+ ++P ++I+++L K
Sbjct: 4 DALKALLQWGASFGVIVPEELKFLYTDLKGIICVCEKDIDNPSIKIPPEIVISRNLPMKF 63
Query: 62 FRDQVKSKEHHNTWXXXXXXXXXXXDEMIILDNENITKLFRPYIMALPSQVDSPLGWNPS 121
F +K + D I+DN + F+PY+ ALPS+++SPL WNPS
Sbjct: 64 FGLSESTKNINGWLKLFFAKIKFDRDNDTIVDNVRVNDKFKPYLDALPSRLNSPLVWNPS 123
Query: 122 ELALLNGTNLYTSLKEKLQSIYDEWWNVIEGTSFQTRQ---------YNIEQLSIDEIYE 172
EL L+ TN+ S+ EK + I+ EW+ ++ + + +N+++L+ + +YE
Sbjct: 124 ELKRLSSTNIGNSIHEKFEGIFKEWFELVSSSDMFDLERVADDVQTFHNLDELTYEALYE 183
Query: 173 QITSKVFSEKILDFFSFPAFLWSHLMFTSRAFPERVINPYCDEYNVILLPVLDLLNHENR 232
+I ++ ++SFPAFLWSHL+F SRAFPE V+N C + +++LLP++DLLNH+ R
Sbjct: 184 KILKITELQRPTIWYSFPAFLWSHLIFISRAFPEYVLNRNCPDNSIVLLPIVDLLNHDYR 243
Query: 233 SKIQWSCSSEGSFIFEKLEPVSKGTEICNNYGAKGNEELLYGYGFVVDGNEFDNLAXXXX 292
SK++W G F +EK+ S+ E+ NNYG KGNEELL GYGFV++ N FD++A
Sbjct: 244 SKVKW-YPENGWFCYEKIGTASQSRELSNNYGGKGNEELLSGYGFVLEDNIFDSVALKVK 302
Query: 293 XXXXXXEKIL-ADGSVYLPXXXXXXXXXXXXVKDTRGNQKKTNDTKPLKALENFADGILY 351
IL + S+ LP KD +KK ++ ++ +G+ Y
Sbjct: 303 LPLDVVSTILETEPSLKLPLLSDYTTYAFEN-KDCVQQEKKAT-----RSATDYINGVTY 356
Query: 352 FINADLEN-LKPLMDLFSLLSKNDNESALSLRCQLTALQSLKNALKQKLYLIIDAEPLES 410
FIN E L+PL+DLF+ LSK + E LR +L +Q L+NAL+ KL I P +
Sbjct: 357 FINIQNEQCLEPLLDLFTYLSKAEEEDLHDLRARLQGIQMLRNALQSKLNSI--TGPPAT 414
Query: 411 QKDYATCEYRKQCAAIYKSGQISILKDSINNIKRLQKHILTAYKPYLASIKSIMKHDRDF 470
YA YR CA +Y GQ ILK+++ +K+L+K +L+ K L ++ I+K+D F
Sbjct: 415 DDSYAIDPYRVYCADVYTKGQKQILKEALTRLKKLEKTMLSENKHQLLTMSKILKNDPAF 474
Query: 471 QKDYHELYDQTNNAAPADLIDE--FEYAVAWLMVRTQKLARSKEASEYLWVQDQFTNYFS 528
+ EL +N ++I E ++ + W++++T+K + ++Y WV Q+TN
Sbjct: 475 AET--ELPSLFSNEDGEEVIFESTYDLLILWILLKTKK---NSYPTKYEWVGQQYTN--- 526
Query: 529 HYTEDSQISNAGKSLHNYFFPNGNDRISVKTMDCIATFINNETFLMESSASNSVPVFTK 587
+ + + IS+ K+ H +F +D + + +D F+ + +F SS + + K
Sbjct: 527 -FKQTAYISDDAKAFHTAYFEKQDD-VDLAEVDHAIQFVVDNSFTRTSSTTEETILVRK 583
>YHL039w [R] KOG1337 N-methyltransferase
Length = 585
Score = 132 bits (332), Expect = 1e-30
Identities = 131/576 (22%), Positives = 238/576 (40%), Gaps = 65/576 (11%)
Query: 3 KLDQLLAFAKDNGVSLPQNVGFKNVEGKGICCIASDDI----EQAVFQLPSHLIITKDLS 58
+LD L +A++NG + + F+ E G+ ++ +QA+ ++P L+IT +
Sbjct: 6 ELDNCLQWAQNNGAFIDPKISFRITEDAGVSAFVNEKFSPKPDQALIRVPETLLITSQQA 65
Query: 59 NKHFRDQVKSKEHHNTWXXXXXXXXXXXDEMIILDNENITKLFRPYIMALPSQVDSPLGW 118
F + N+ + + ++ ++PY+ LP + P W
Sbjct: 66 LSEFSQAANERSLLNSVTQLYLSKLKFGTDAV-----HLKSFYKPYLDVLPLHLPQPYFW 120
Query: 119 NPSELALLNGTNLYTSLKEKLQSIYDEWWNVIEGTSFQTRQYNIEQLSIDE--------I 170
+ E+ L+GT++Y ++++ L + EW + + S + + + LS+ +
Sbjct: 121 STDEVMNLHGTDVYLTMRDTLNKLVKEWRMLFQALSIEHSSQDKQFLSLFQENKDSAVVP 180
Query: 171 YEQITSKVFSEKILD--FFSFPAFLWSHLMFTSRAFPERVI-NPYCDEYNV---ILLPVL 224
EQ + + K+ D + SF A+LWS+ +F SRAFP ++ D N+ L P++
Sbjct: 181 LEQFCAHINGCKLEDSEWNSFVAYLWSYCIFNSRAFPRVILGRAGTDRTNLNEGFLYPIV 240
Query: 225 DLLNHENRSKIQWSCSSEGSFIF-EKLEPVSKGTEICNNYGAKGNEELLYGYGFVVDGNE 283
DLLNH+N ++W + + F + S E+ NNYG NE+ L YGF N+
Sbjct: 241 DLLNHKNDVPVRWEMNEQNELCFMSQTTTFSAQDELFNNYGNISNEKCLLNYGFWDSSNK 300
Query: 284 FDNLAXXXXXXXXXXEKILADGSVYLPXXXXXXXXXXXXVKDTRGNQKKTNDTKPLKALE 343
FD L G LP V + T+D +
Sbjct: 301 FD-----FSRLTLKLPSTLVSG---LP------------VDFNKSGNFVTDDGETT---- 336
Query: 344 NFADGILYFINADLENLKP-LMDLFSLLSKNDNESALSLRCQLTALQSLKNALKQKLYLI 402
IL F E L P L+ LF+ LSK +E ++R L + L + + Q+L L
Sbjct: 337 -----ILQFSLKISEPLPPVLLALFAYLSKLKSEETPTVRSVLEGIDQLTSVVSQRL-LF 390
Query: 403 IDAEPLESQKDYATCEYRKQCAAIYKSGQISILKDSINNIKRLQKHILTAYKPYLASIKS 462
+++ + + +Y IL + + LQK I + K + S K+
Sbjct: 391 YKNFKIKTSSTQKLRPHVIKLIKLYYQDNKKILNATTEKLSVLQKKIYSNNKEFSLSFKT 450
Query: 463 IMKHDRDFQKDYHELYDQTNNAAPADLIDE---FEYAVAWLMVRTQKLARSKEASEYLWV 519
I K+D+ F ++ N DLI + + + W++ KL K ++ ++
Sbjct: 451 IFKNDKIFANSLLLVFGAINY---EDLITKDCLNDALLLWIV----KLINDKSNNQGGFI 503
Query: 520 QDQFTNYFSHYTEDSQISNAGKSLHNYFFPNGNDRI 555
+ F + + + +FPN ++RI
Sbjct: 504 KQTFKEVSDSIVIEKEDVMEFLPFYKKYFPNLSERI 539
>SPBC1709.13c [R] KOG1337 N-methyltransferase
Length = 547
Score = 104 bits (260), Expect = 3e-22
Identities = 118/463 (25%), Positives = 187/463 (39%), Gaps = 66/463 (14%)
Query: 4 LDQLLAFAKDNGVSLPQNVGFKNVEGKGIC-----CIASDDI--EQAVFQLPSHLIITKD 56
+++LL A NG L ++V F C +A +DI +Q + P IT +
Sbjct: 1 MEKLLHEALQNGCKLHKSVEFIQSRDDNACFGSYIAVAQNDIAPDQLLISCPFEYAITYN 60
Query: 57 LSNKHFRDQVKSKEHHNTWXXXXXXXXXXXDEMIILDNENITKLFRPYIMALPSQVDSPL 116
+ + + + E N + + + I + YI LP ++PL
Sbjct: 61 KAKEELKKLNPNFESCNPHITLCTF-------LALESLKGIQSKWYGYIEYLPKTFNTPL 113
Query: 117 GWNPSELALLNGTNLYTSLKEKLQSIYDEWWNVIEGTSFQTRQYNIEQLSIDEIYEQITS 176
+N ++ A L TN Y++ +E+L E+ + T +
Sbjct: 114 YFNENDNAFLISTNAYSAAQERLHIWKHEYQEALSLHPSPTER----------------- 156
Query: 177 KVFSEKILDFFSFPAFLWSHLMFTSRAFPERVINPYCD-EYNVILLPVLDLLNHENRSKI 235
F+F ++WS +F+SR F +I Y D E ILLP++D LNH+ + I
Sbjct: 157 ----------FTFDLYIWSATVFSSRCFSSNLI--YKDSESTPILLPLIDSLNHKPKQPI 204
Query: 236 QWSC--SSEGSFIFEKLEPVSKGTEICNNYGAKGNEELLYGYGFVVDGNEFDNLAXXXXX 293
W+ E S E V+KG ++ NNYG KGNEELL GYGF + N FD +
Sbjct: 205 LWNSDFQDEKSVQLISQELVAKGNQLFNNYGPKGNEELLMGYGFCLPDNPFDTVTLKVAI 264
Query: 294 XXXXXEKILADGSVYLPXXXXXXXXXXXXVKDTRGNQKKTNDTKPLKALENFADGILYFI 353
LP + N P K + L +
Sbjct: 265 HPD------------LPHKDQKAAILENDCQFQLSNLVFFLPKSPDKEIFQKILQCLAVV 312
Query: 354 NADLENLKPLMDLFSLLSKNDNESALSLRCQLTALQSLKNALKQKLYLIIDAEPLESQKD 413
A L+ L LL+ + SLR Q+ +L+ L + + L++ + P S
Sbjct: 313 TASSLELRKL--TAHLLTGDLASYVPSLRGQIKSLEVLLMYIDSRADLLLKSNPQVS--- 367
Query: 414 YATCEYRKQCAAIYKSGQISILKDSINNIKR-LQKHILTAYKP 455
T E R+ A IY+ QI+IL+DSI +K +++ + YKP
Sbjct: 368 -PTSE-RQVWAKIYRDSQINILQDSITYVKNYMEESLQKTYKP 408
>SPAC3C7.09 [R] KOG1337 N-methyltransferase
Length = 429
Score = 62.8 bits (151), Expect = 1e-09
Identities = 45/182 (24%), Positives = 84/182 (45%), Gaps = 35/182 (19%)
Query: 98 TKLFRPYIMALPSQVDSPLGWNPSELALLNGTNLYTSLKEKLQSIYDEWWNVIEGTSFQT 157
T + ++ LP +++P W E+ L GT+++ + K + + EW
Sbjct: 100 TNKWDKFLTVLPLSINTPAQWPEKEVYSLQGTSIFNPVCVKRKILQQEW----------- 148
Query: 158 RQYNIEQLSIDEIYEQITSKVFSEKILDFFSFPAFLWSHLMFTSRAFPERVINPYCDEYN 217
LS+++ Y S + KI + P ++ + +F SR +P
Sbjct: 149 -------LSLNQRY----SDSWPSKI----TLPKWVHADALFHSRCLESPFKDP------ 187
Query: 218 VILLPVLDLLNHENRSKIQWSCSSEGSFIFEKLEPVSKGTEICNNYGA-KGNEELLYGYG 276
+L PV+DL NH ++S +WS S + ++ + + + E+ NYG+ KG+ E L+ YG
Sbjct: 188 -VLAPVIDLCNHSSKSNAKWSFSEDAMQLYLD-KDIDENEEVTINYGSEKGSAEFLFSYG 245
Query: 277 FV 278
F+
Sbjct: 246 FL 247
>At3g07670 [R] KOG1337 N-methyltransferase
Length = 504
Score = 51.2 bits (121), Expect = 4e-06
Identities = 55/181 (30%), Positives = 78/181 (42%), Gaps = 36/181 (19%)
Query: 104 YIMALPSQVDSPLGWNPSELALLNGTNLYTSLKEKLQSIYDEWWNVIEGTSFQTRQYNIE 163
YI ALP Q S L W +EL ++Y + + + NV+ GT
Sbjct: 169 YISALPRQPYSLLYWTRTEL------DMYLEASQIRERAIERITNVV-GT---------- 211
Query: 164 QLSIDEIYEQITSKVFSEKILDF----FSFPAFLWSHLMFTSRAFPERVINPYCDEYNVI 219
YE + S++FS+ F F+ F WS + SR V P D
Sbjct: 212 -------YEDLRSRIFSKHPQLFPKEVFNDETFKWSFGILFSRL----VRLPSMDG-RFA 259
Query: 220 LLPVLDLLNH--ENRSKIQWSCSSEGSFIFEKLEPVSKGTEICNNYGAKGNEELLYGYGF 277
L+P D+LNH E + + + SS+G +F P G ++ +YG K N ELL YGF
Sbjct: 260 LVPWADMLNHNCEVETFLDYDKSSKG-VVFTTDRPYQPGEQVFISYGNKSNGELLLSYGF 318
Query: 278 V 278
V
Sbjct: 319 V 319
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,507,288
Number of Sequences: 60738
Number of extensions: 1445710
Number of successful extensions: 3524
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3503
Number of HSP's gapped (non-prelim): 8
length of query: 594
length of database: 30,389,216
effective HSP length: 112
effective length of query: 482
effective length of database: 23,586,560
effective search space: 11368721920
effective search space used: 11368721920
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)