ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0630 good U KOG2760 Intracellular trafficking, secretion, and vesicular transport Vacuolar sorting protein VPS36
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0630 222636 221107 -510
(510 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR417w [U] KOG2760 Vacuolar sorting protein VPS36 461 e-130
SPBC3B9.09 [U] KOG2760 Vacuolar sorting protein VPS36 151 3e-36
At5g04920 [U] KOG2760 Vacuolar sorting protein VPS36 57 5e-08
Hs7705640 [U] KOG2760 Vacuolar sorting protein VPS36 56 1e-07
7294458 [U] KOG2760 Vacuolar sorting protein VPS36 51 4e-06
>YLR417w [U] KOG2760 Vacuolar sorting protein VPS36
Length = 566
Score = 461 bits (1187), Expect = e-130
Identities = 259/565 (45%), Positives = 362/565 (63%), Gaps = 68/565 (12%)
Query: 7 YWHPVEIAGSGQPILRENERDIYVEQSVGLYHGKTKILNKQKGRLYLTSQRIIYVDELAP 66
YWH VE SGQP+LRE E+DI+++QSVGLYHGK+KIL +Q+GR++LTSQRIIY+D+ P
Sbjct: 3 YWHYVETTSSGQPLLREGEKDIFIDQSVGLYHGKSKILQRQRGRIFLTSQRIIYIDDAKP 62
Query: 67 KKESVCIENDDIMNVEYSSKFLKRSAKIILFFKELSQNEAPIEEKGKQVHT------TQW 120
+ S+ +E DD+ V YSS FL RS ++ILFFK+ S +E GK T + W
Sbjct: 63 TQNSLGLELDDLAYVNYSSGFLTRSPRLILFFKDPSSK----DELGKSAETASADVVSTW 118
Query: 121 NCPICGVENRIYSTLDSNGLNMPPCGSCGVEVDRDMVQNSIK-TVVEDLEETPK------ 173
CPIC V N + L P C +CGV D ++ ++SI + D P+
Sbjct: 119 VCPICMVSNETQGEFTKDTLPTPICINCGVPADYELTKSSINCSNAIDPNANPQNQFGVN 178
Query: 174 -ENICPVCTFINHPQIRNCEICGARL-KQSRRTSKVK-----DSRVRILLEEKTPSGEES 226
ENICP CTF NHPQI NCEICG RL S+ SK+ DSRV I LE+ + + +S
Sbjct: 179 SENICPACTFANHPQIGNCEICGHRLPNASKVRSKLNRLNFHDSRVHIELEKNSLARNKS 238
Query: 227 ----------------FAQLSFRKTDGALFYETLVKQLQEISRKESKHLFNQNVLSVNGV 270
F QLSFRK+DG LF + + L+ I +++KH+FNQNV+SVNGV
Sbjct: 239 SHSALSSSSSTGSSTEFVQLSFRKSDGVLFSQATERALENILTEKNKHIFNQNVVSVNGV 298
Query: 271 -----PTDPVIDPDLVLSNNDINLIGITALEKNKEQQLLKNNIVMSNALSDLNKLMALAE 325
+ + ++ ++ IGI++LEK++E QLL N+I+ +NAL+DLNKLM+LA
Sbjct: 299 DMRKGASSHEYNNEVPFIETKLSRIGISSLEKSRENQLLNNDILFNNALTDLNKLMSLAT 358
Query: 326 DIEKMYQGN-----------------RKSKSNESILLVDREKYLNKNVFLEEIARELYGF 368
IE++Y+ + + K+ +L++DREK+LNK +FL+EIARE+Y F
Sbjct: 359 SIERLYKNSNITMKTKTLNLQDESTVNEPKTRRPLLILDREKFLNKELFLDEIAREIYEF 418
Query: 369 VDSE---LRGDSG-IIVTLVDLFALYNKSVRIGTGLISPEEMREACEKFEKLGLNDLKLI 424
SE L D+ +I+TLVDL+A+YNKS+RIGTGLISP EMREACE+FE LGLN+LKL+
Sbjct: 419 TLSEFKDLNSDTNYMIITLVDLYAMYNKSMRIGTGLISPMEMREACERFEHLGLNELKLV 478
Query: 425 KINGRVLCLAFQDSFDHIRTKVLQLATMRPGVDILQITTELNV-YEKNNWSMGVISEVLQ 483
K+N R+LC+ + FD ++ K++ L PG D+L++T L+ K+NW++G++ EVLQ
Sbjct: 479 KVNKRILCVT-SEKFDVVKEKLVDLIGDNPGSDLLRLTQILSSNNSKSNWTLGILMEVLQ 537
Query: 484 YCVAEGNLVMDEQISGTNYYFNSYW 508
CV EG+L++D+Q+SG YY NSYW
Sbjct: 538 NCVDEGDLLIDKQLSGIYYYKNSYW 562
>SPBC3B9.09 [U] KOG2760 Vacuolar sorting protein VPS36
Length = 467
Score = 151 bits (381), Expect = 3e-36
Identities = 132/523 (25%), Positives = 220/523 (41%), Gaps = 101/523 (19%)
Query: 11 VEIAGSGQPILRENERDIYVEQSVGLYHGKTKILNKQKGRLYLTSQRIIYVDELAPKKES 70
+E S P+L +E + V+ VGLY G K G LYLT + I YVD + PKK S
Sbjct: 5 LETTPSNLPVLDPSEEILLVQDQVGLYFGDEKSFRHNNGTLYLTKKHIFYVDSVDPKKNS 64
Query: 71 VCIENDDIMNVEYSSKFLKRSAKIILFFKELSQNEAPIEEKGKQVHTTQWNCPICGVENR 130
+ I+ DI +V+++S++ + S KI L + + ++ W C IC
Sbjct: 65 LKIKISDIRDVQHTSRYFRSSPKIRLSLRHVEKS---------------WACKIC----- 104
Query: 131 IYSTLDSNGLNMPPCGSCGVEVDRDMVQNSIKTVVEDLEETPKENICPVCTFINHPQIRN 190
T + G + PC +CG V N + + + +C CTF N+P +
Sbjct: 105 ---TFINVGDPINPCRNCG-------VANRFTIIKPKSDARFSQGLCTACTFQNYPDLNT 154
Query: 191 CEICGARLKQSRRTSKVKDSRVRILLEEKTPSGEESFAQLSFRKTDGALFYETLVKQLQE 250
CEICG +LK R + QLSFR + + FYE + + E
Sbjct: 155 CEICGNQLKNVDRNQLI---------------------QLSFRGSGSSKFYEAIKSETDE 193
Query: 251 ISRKESKHLFNQNVLSVNGVPTDPVIDPDLVLSNNDINLIGITALEKNKEQQLLKNNIVM 310
I ++ + ++ VL + + + GI LE++ E QL KN +
Sbjct: 194 IEKQRYSNKYDTKVLR-------------KAILEKSLRMGGIHDLEQSHEMQLAKNGRTL 240
Query: 311 SNALSDLNKLMALAED--------IEKMYQGNRKSKSNESILLVDREKYL---------- 352
+A DL+ +LA+D EKM +S++ L+++ L
Sbjct: 241 VHAFQDLDAFFSLAKDTMSLADQFAEKMDGLTGTQQSDKVQQLLNKSNQLGVLRGNHLDN 300
Query: 353 -----NKNVFLEEIARELYGFVDSELRGDSGIIVTLVDLFALYNKSVRIGTGLISPEEMR 407
N ++ E+ + + + + L+ D+ VT+ +A+YN+S + LI P
Sbjct: 301 VPVSANSRLYDIELCKSISEVLRTRLKADTD-TVTMTQAWAIYNRSRK--ANLIPPTRFV 357
Query: 408 EACEKFEKLGLNDLKLIKINGRVLCLAFQDSFDHIRTKVLQLATMRPGVDILQITTELNV 467
+ACE + + + + K+N ++ + S + L M P TT L
Sbjct: 358 KACELIDSMEVG-ITTSKMNSGLILFQLKTSNHSGKLLSAILEVMNPS------TTALKC 410
Query: 468 YEKNNWSMGVISEVLQYCVAEGNLVMD-EQISGTNYYFNSYWK 509
+ +WS+GV E L +G +V D + SG++ YWK
Sbjct: 411 AMRLHWSIGVTLERLYQAEMDGYIVRDVYEESGSSLL---YWK 450
>At5g04920 [U] KOG2760 Vacuolar sorting protein VPS36
Length = 440
Score = 57.4 bits (137), Expect = 5e-08
Identities = 58/255 (22%), Positives = 114/255 (43%), Gaps = 40/255 (15%)
Query: 283 SNNDINLIGITALEKNKEQQLLKNNIVMSNALSDLNKLMALAEDI----EKMYQGNRKSK 338
++ + ++G+ + + +++Q + + +A DLN LM+ A+++ EKM Q +
Sbjct: 167 NDGTVRMVGLAGILRKEQEQWESTDKSLQDAFQDLNALMSKAKEMVSLAEKMRQKLLSAP 226
Query: 339 SNESILLVDRE---------------------KYLNKNVFLEEIARELYGFVDSELRGDS 377
S+++ D E K ++ +E++R+L FV L
Sbjct: 227 SSQNGSTDDEEMGSKEEMQQWMLSVGIISPVTKESAGALYHQELSRQLADFVRIPLEKAG 286
Query: 378 GIIVTLVDLFALYNKSVRIGTGLISPEEMREACEKFEKLGLNDLKLIKINGRVLCL---- 433
G+I +L D++ +N++ GT LISP+++ +AC +EK + + L K + V+ +
Sbjct: 287 GMI-SLTDMYYHFNRA--RGTELISPDDLWQACTLWEKFDV-PVMLRKFDSGVMVIQNKS 342
Query: 434 -AFQDSFDHIRTKVLQLATMRPGVDILQITTELNVYEKNNWSMGVISEVLQYCVAEGNLV 492
+ ++ IR V + T+R GV L + + + E L +G L
Sbjct: 343 HSDEEVMSRIRMLVTKTETLRVGVTASDAALTLKI------APAMAKEHLLSAETKGLLC 396
Query: 493 MDEQISGTNYYFNSY 507
D G +YFN +
Sbjct: 397 RDMSPDGLRFYFNLF 411
>Hs7705640 [U] KOG2760 Vacuolar sorting protein VPS36
Length = 386
Score = 55.8 bits (133), Expect = 1e-07
Identities = 69/306 (22%), Positives = 134/306 (43%), Gaps = 43/306 (14%)
Query: 222 SGEESFAQLSFRKTDGALFYETLVKQLQEISRKESKHLFNQNVLSVNGVPTDPVIDPDLV 281
S + S+ +LSF++ FY L +E++++ +++ L N P
Sbjct: 100 SSKNSYIKLSFKEHGQIEFYRRLS---EEMTQRRWENMPVSQSLQTNRGPQP-------- 148
Query: 282 LSNNDINLIGITALEKNKEQQLLKNNIVMSNALSDLNKLMALAE---DIEKMYQGNRKSK 338
I +GI +E+ E++ + + +S A DL+KLM A+ ++ K K K
Sbjct: 149 ---GRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDK 205
Query: 339 SNE----------SILL-------VDREKYLNKNVFLEEIARELYGFVDSELRGDSGIIV 381
+ S LL V RE Y + + ++A++L G + L + G I+
Sbjct: 206 QGDITEDETIRFKSYLLRMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLE-ERGGIM 264
Query: 382 TLVDLFALYNKSVRIGTGLISPEEMREACEKFEKLGLNDLKLIKINGRVLCLAFQDSFDH 441
+L +++ L N++ G L+SPE++ AC+ E L L L+L + V+ + Q
Sbjct: 265 SLTEVYCLVNRA--RGMELLSPEDLVNACKMLEALKL-PLRLRVFDSGVMVIELQSH--- 318
Query: 442 IRTKVLQLATMRPGVDILQITTELNVYEKNNWSMGVISEVLQYCVAEGNLVMDEQISGTN 501
+ + + + + + +T+E + S+ + E L G+L D+ + G
Sbjct: 319 -KEEEMVASALETVSEKGSLTSE-EFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLR 376
Query: 502 YYFNSY 507
+Y N +
Sbjct: 377 FYPNLF 382
>7294458 [U] KOG2760 Vacuolar sorting protein VPS36
Length = 399
Score = 51.2 bits (121), Expect = 4e-06
Identities = 56/263 (21%), Positives = 115/263 (43%), Gaps = 40/263 (15%)
Query: 267 VNGVPTDPVIDPDLVLSNNDINLI----GITALEKNKEQQLLKNNIVMSNALSDLNKLMA 322
+NGV + P ++P +N+ + I GI +E++ E + + ++ A DL+ LMA
Sbjct: 147 INGVASSPTLEP----ANDRLARIQKRTGIGGIERHLEAKAKATDENIALAFQDLSVLMA 202
Query: 323 LAEDI--------EKMYQGNRKSKSNESILL------------VDREKYLNKNVFLEEIA 362
+A+D+ K+ + + +E++ V R+ + + + + +A
Sbjct: 203 MAKDMVGVSKTISSKIREQKGEISDDETVRFKSYLMSLGIDDPVTRDNFTSNSAYFSSLA 262
Query: 363 RELYGFVDSELRGDSGIIVTLVDLFALYNKSVRIGTGLISPEEMREACEKFEKLGLNDLK 422
R++ + + + G +++L D++ N++ G L+SPE++ ACE+ G L+
Sbjct: 263 RQICEMLLDPIE-EQGGMMSLADVYCRVNRA--RGLELLSPEDLLHACEQLS--GPIRLR 317
Query: 423 LIKINGRVLCLAFQDSFDHIRTKVLQLATMRPGVDILQITTELNVYEKNNWSMGVISEVL 482
RVL L D I L+ + + ++ +L + S+ + E L
Sbjct: 318 SFPSGARVLQLESHDD-ALIAVDTLEKVEAAESLAVEELAKQLGI------SLLLAKERL 370
Query: 483 QYCVAEGNLVMDEQISGTNYYFN 505
G + DE + G +Y N
Sbjct: 371 LVAERLGKVCRDESVEGLRFYPN 393
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,800,650
Number of Sequences: 60738
Number of extensions: 1354318
Number of successful extensions: 4367
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4348
Number of HSP's gapped (non-prelim): 6
length of query: 510
length of database: 30,389,216
effective HSP length: 111
effective length of query: 399
effective length of database: 23,647,298
effective search space: 9435271902
effective search space used: 9435271902
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)