ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactII0743   suspect: LH   S     KOG4843  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactII0743  263420 262458 -321 
         (321 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL076c [S] KOG4843 Uncharacterized conserved protein                 165   9e-41
SPCC1259.07 [S] KOG4843 Uncharacterized conserved protein              61   2e-09

>YDL076c [S] KOG4843 Uncharacterized conserved protein
          Length = 294

 Score =  165 bits (417), Expect = 9e-41
 Identities = 117/323 (36%), Positives = 167/323 (51%), Gaps = 48/323 (14%)

Query: 6   YRKTQNRIYSMQETLLNSSRESKQARDQDTGKIGXXXXXXXXXXXXNTQNVADESSQTGE 65
           YR+TQ++IY +QETLLNS+R                          N Q    ES+    
Sbjct: 12  YRETQSQIYKLQETLLNSARTK------------------------NKQEEGQESNTHSF 47

Query: 66  PENNTTLETAAPDSKPEDDEDEIDVNSKSVLEYTEKNYALQRRPLATLEYDSMRVGVFPG 125
           PE     +     +      D  +V+S+SVL +TEK+Y  + + L TL Y+  + G F  
Sbjct: 48  PEQYMHYQNGRNSAY-----DLPNVSSQSVLAFTEKHYPNKLKNLGTLYYNRFKEGSFDE 102

Query: 126 DPKL--EALPFPYNFKTIRSPRPFIPRMSHNHINSILTIQIDSIYLQQVARS-ENIRYQN 182
           D     +   FPYN      P PF+P +   +IN+I T++I    +Q    + E+ R +N
Sbjct: 103 DSTSYSDRHSFPYNLYDNTLPPPFLPAIGIQNINNIATLKITYEDIQASFNNIESPRKRN 162

Query: 183 REIWGTDIYTDDSDIILVLQHCGVLPFSEPLDQNSNGKPSNVHSLKTKLRRTPTNMSDQN 242
            EIWG DIY+DDSD ILVL+HCG             G PS       KLRRTP N+++Q+
Sbjct: 163 NEIWGCDIYSDDSDPILVLRHCGF----------KIGAPSG--GSFHKLRRTPVNVTNQD 210

Query: 243 SVTGAIPERE-IPFAIKVDILMLPPLQGYASSTRNNITSREWTGQ---VHDGLSFGIFHV 298
           +VTG +P  E  PF ++V++L LP LQ Y S  R +ITSREW  +   +HDGLS+GI+ +
Sbjct: 211 NVTGNLPLLEGTPFDLEVELLFLPTLQKYPSVKRFDITSREWGSEATVIHDGLSYGIYSI 270

Query: 299 DIVVMDNTLNEIQQSDKTKSLKW 321
            I    +     + +   K+LKW
Sbjct: 271 VIKQRLDRDKPHEPNGYIKNLKW 293


>SPCC1259.07 [S] KOG4843 Uncharacterized conserved protein
          Length = 351

 Score = 60.8 bits (146), Expect = 2e-09
 Identities = 44/146 (30%), Positives = 61/146 (41%), Gaps = 44/146 (30%)

Query: 153 HNHINSILTIQIDSIYLQQVARSENIRYQNREIWGTDIYTDDSDIILVLQHCGVLPFSEP 212
           ++ I   + + ID  +L       N R   REIWGTD+YTDDSDI  +L HCG     +P
Sbjct: 213 YSSIGKFVRVTIDIKWLNAAI---NPRLCRREIWGTDVYTDDSDIATILAHCGCFSLLKP 269

Query: 213 LDQNSNGKPSNVHSLKTKLRRTPTNMSDQNSVTGAIPEREIPFAIKVDILMLPPLQGYAS 272
           +                                     R+I     VD+ +LPPL  Y  
Sbjct: 270 V-------------------------------------RKIAV---VDLYILPPLVHYKG 289

Query: 273 STRNNITSREWTGQVHDGLSFGIFHV 298
           + +N I SR W+ +  DG+S  I  V
Sbjct: 290 TRKNQIESRSWSSR-QDGISLKIKEV 314


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.312    0.131    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,073,295
Number of Sequences: 60738
Number of extensions: 827779
Number of successful extensions: 2345
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2336
Number of HSP's gapped (non-prelim): 3
length of query: 321
length of database: 30,389,216
effective HSP length: 106
effective length of query: 215
effective length of database: 23,950,988
effective search space: 5149462420
effective search space used: 5149462420
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)