ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0862 good R KOG2978 General function prediction only Dolichol-phosphate mannosyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0862 305323 306117 265
(265 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR183w [R] KOG2978 Dolichol-phosphate mannosyltransferase 397 e-111
Hs4503363 [R] KOG2978 Dolichol-phosphate mannosyltransferase 120 3e-27
At1g20570_1 [R] KOG2978 Dolichol-phosphate mannosyltransferase 111 9e-25
CE22753 [R] KOG2978 Dolichol-phosphate mannosyltransferase 108 8e-24
SPAC31G5.16c [R] KOG2978 Dolichol-phosphate mannosyltransferase 106 3e-23
7298600 [R] KOG2978 Dolichol-phosphate mannosyltransferase 105 5e-23
ECU04g1060 [R] KOG2978 Dolichol-phosphate mannosyltransferase 85 9e-17
YPL227c [R] KOG2977 Glycosyltransferase 50 3e-06
>YPR183w [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 267
Score = 397 bits (1020), Expect = e-111
Identities = 199/267 (74%), Positives = 224/267 (83%), Gaps = 2/267 (0%)
Query: 1 MSIENSVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQ 60
MSIE SV+VPAYHEKLNIKPLTTRLF + E + TELIFVDDNSQDGSVEEV+AL Q
Sbjct: 1 MSIEYSVIVPAYHEKLNIKPLTTRLFAGMSPEMAKKTELIFVDDNSQDGSVEEVDALAHQ 60
Query: 61 GYNVRIIVRTDERGLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLLDSLRTHPFVLG 120
GYNVRIIVRT+ERGLSSAVLKGF+EAKG+YLVCMDADLQHPPE+VP+L +SL H F LG
Sbjct: 61 GYNVRIIVRTNERGLSSAVLKGFYEAKGQYLVCMDADLQHPPETVPKLFESLHDHAFTLG 120
Query: 121 TRYAAGVGIDKDWPLYRRVISSGARAMAKPLTSASDPMSGFFGLQKKYLIHKDAKNINPK 180
TRYA GVGIDKDWP+YRRVISS AR MA+PLT ASDPMSGFFGLQKKYL + + ++IN +
Sbjct: 121 TRYAPGVGIDKDWPMYRRVISSTARMMARPLTIASDPMSGFFGLQKKYLENCNPRDINSQ 180
Query: 181 GFKIALDLLVKLPIP--PQEPIGEVPFFFGVRTEGESKLSGKVIIQYLHQLRELYTYKYG 238
GFKIAL+LL KLP+P P+ IGEVPF FGVRTEGESKLSGKVIIQYL QL+ELY +K+G
Sbjct: 181 GFKIALELLAKLPLPRDPRVAIGEVPFTFGVRTEGESKLSGKVIIQYLQQLKELYVFKFG 240
Query: 239 GNNXXXXXXXWTILALYILYQAYHLVF 265
NN W+IL Y+ YQ YHLVF
Sbjct: 241 ANNLILFITFWSILFFYVCYQLYHLVF 267
>Hs4503363 [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 260
Score = 120 bits (300), Expect = 3e-27
Identities = 81/234 (34%), Positives = 128/234 (54%), Gaps = 10/234 (4%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNVR 65
SV++P Y+E+ N+ + L ++ + G N E+I +DD S DG+ + E L+K + R
Sbjct: 28 SVLLPTYNERENLPLIVWLLVKSFSESGINY-EIIIIDDGSPDGTRDVAEQLEKIYGSDR 86
Query: 66 IIVRTDER--GLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLLDSLRTHPF--VLGT 121
I++R E+ GL +A + G A G Y++ MDADL H P+ +P+ + + F V GT
Sbjct: 87 ILLRPREKKLGLGTAYIHGMKHATGNYIIIMDADLSHHPKFIPEFIRKQKEGNFDIVSGT 146
Query: 122 RYAAGVGIDKDWPLYRRVISSGARAMAKPL--TSASDPMSGFFGLQKKYLIHKDAKNINP 179
RY G+ W L R++IS GA + + L ASD ++G F L +K ++ K +
Sbjct: 147 RYKGNGGV-YGWDLKRKIISRGANFLTQILLRPGASD-LTGSFRLYRKEVLEKLIEKCVS 204
Query: 180 KGFKIALDLLVKLPIPPQEPIGEVPFFFGVRTEGESKLSGKVIIQYLHQLRELY 233
KG+ ++++V+ IGEVP F R GESKL G I+ +L L L+
Sbjct: 205 KGYVFQMEMIVRAR-QLNYTIGEVPISFVDRVYGESKLGGNEIVSFLKGLLTLF 257
>At1g20570_1 [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 242
Score = 111 bits (278), Expect = 9e-25
Identities = 81/231 (35%), Positives = 123/231 (53%), Gaps = 12/231 (5%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNVR 65
S+++P Y+E+LNI + +F+ L E+I VDD S DG+ E V+ L++ R
Sbjct: 16 SIIIPTYNERLNIAIIVYLIFKHLRDVDF---EIIVVDDGSPDGTQEIVKQLQQLYGEDR 72
Query: 66 IIVRTDER--GLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLL-DSLRTH-PFVLGT 121
I++R + GL +A + G A G+++V MDADL H P+ +P + L T+ V GT
Sbjct: 73 ILLRARAKKLGLGTAYIHGLKHATGDFVVIMDADLSHHPKYLPSFIKKQLETNASIVTGT 132
Query: 122 RYAAGVGIDKDWPLYRRVISSGARAMAKPL--TSASDPMSGFFGLQKKYLIHKDAKNINP 179
RY G G+ W L R++ S GA +A+ L SD ++G F L KK + +
Sbjct: 133 RYVKGGGV-HGWNLMRKLTSRGANVLAQTLLWPGVSD-LTGSFRLYKKSALEDVISSCVS 190
Query: 180 KGFKIALDLLVKLPIPPQEPIGEVPFFFGVRTEGESKLSGKVIIQYLHQLR 230
KG+ ++++V+ I EVP F R G SKL G I++YL LR
Sbjct: 191 KGYVFQMEMIVRATRKGYH-IEEVPITFVDRVFGTSKLGGSEIVEYLKGLR 240
>CE22753 [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 244
Score = 108 bits (270), Expect = 8e-24
Identities = 77/235 (32%), Positives = 126/235 (52%), Gaps = 10/235 (4%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNVR 65
S+++P Y+EK N+ P+ L + KE S+ E+I VDD S DG+ + L+K+ + +
Sbjct: 13 SIILPTYNEKENL-PICIWLIENYLKEVSH--EVIIVDDASPDGTQGIAKLLQKEYGDDK 69
Query: 66 IIV--RTDERGLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLLDSLRTH--PFVLGT 121
I++ R + GL +A G A+GE+++ MDADL H P+ +P+++ + V GT
Sbjct: 70 ILIKPRVGKLGLGTAYSHGLSFARGEFIILMDADLSHHPKFIPEMIALQHKYKLDIVTGT 129
Query: 122 RYAAGVGIDKDWPLYRRVISSGARAMAKPLTSAS-DPMSGFFGLQKKYLIHKDAKNINPK 180
RY G G+ W L R+ IS GA +A+ L + ++G F L K+ ++ K K
Sbjct: 130 RYKDGGGV-SGWDLKRKTISKGANFLAQFLLNPGVSDLTGSFRLYKRDILSKLIAESVSK 188
Query: 181 GFKIALDLLVKLPIPPQEPIGEVPFFFGVRTEGESKLSGKVIIQYLHQLRELYTY 235
G+ ++++ + IGEVP F R GESKL + I+ Y L L+ +
Sbjct: 189 GYVFQMEMMFRAKKSGYR-IGEVPISFVDRFFGESKLGSQEIVDYAKGLLYLFAF 242
>SPAC31G5.16c [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 236
Score = 106 bits (265), Expect = 3e-23
Identities = 74/227 (32%), Positives = 123/227 (53%), Gaps = 10/227 (4%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNVR 65
SV++P Y+E+ N+ +T + + +E + E++ +DD S DG+ E + L+K +
Sbjct: 5 SVLLPTYNERKNLPIITYLIAKTFDQEKLD-WEIVIIDDASPDGTQEVAKELQKIYGEDK 63
Query: 66 IIV--RTDERGLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLLDSLRTHPF--VLGT 121
I++ R+ + GL +A + G A G++++ MDAD H P+ +P+ + + H + VLGT
Sbjct: 64 ILLKPRSGKLGLGTAYIHGLKFATGDFVIIMDADFSHHPKYLPEFIKLQKEHNYDIVLGT 123
Query: 122 RYAAGVGIDKDWPLYRRVISSGARAMAKPL--TSASDPMSGFFGLQKKYLIHKDAKNINP 179
RYA G+ W L R+ IS GA +A + T SD ++G F L KK ++ +
Sbjct: 124 RYAKDGGV-YGWNLKRKFISRGANLLASTVLGTGVSD-VTGSFRLYKKPVLETLMSEVTS 181
Query: 180 KGFKIALDLLVKLPIPPQEPIGEVPFFFGVRTEGESKLSGKVIIQYL 226
KG+ ++++ + IGEVP F R GESKL I+ YL
Sbjct: 182 KGYVFQMEIIARAR-EHNYTIGEVPIAFVDRLYGESKLGMDDILGYL 227
>7298600 [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 241
Score = 105 bits (263), Expect = 5e-23
Identities = 78/234 (33%), Positives = 124/234 (52%), Gaps = 10/234 (4%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNVR 65
S+++P Y+EK N+ P+ L K E+I +DD S DG+++ + L+K +
Sbjct: 9 SILMPTYNEKDNL-PIIIWLIVKYMKASGLEYEVIVIDDGSPDGTLDVAKDLQKIYGEDK 67
Query: 66 IIVRT--DERGLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQL--LDSLRTHPFVLGT 121
I++R + GL +A + G A G+++V +DADL H P+ +P+ L + V GT
Sbjct: 68 IVLRPRGSKLGLGTAYIHGIKHATGDFIVIIDADLSHHPKFIPEFIKLQQEGNYDIVSGT 127
Query: 122 RYAAGVGIDKDWPLYRRVISSGARAMAKPL--TSASDPMSGFFGLQKKYLIHKDAKNINP 179
RYA G+ W R++IS GA +++ L +ASD ++G F L KK ++ K +
Sbjct: 128 RYAGNGGV-FGWDFKRKLISRGANFLSQVLLRPNASD-LTGSFRLYKKDVLEKCIASCVS 185
Query: 180 KGFKIALDLLVKLPIPPQEPIGEVPFFFGVRTEGESKLSGKVIIQYLHQLRELY 233
KG+ +++LV+ I EVP F R G SKL G IIQ+ L L+
Sbjct: 186 KGYVFQMEMLVRAR-QHGYTIAEVPITFVDRIYGTSKLGGTEIIQFAKNLLYLF 238
>ECU04g1060 [R] KOG2978 Dolichol-phosphate mannosyltransferase
Length = 230
Score = 85.1 bits (209), Expect = 9e-17
Identities = 63/230 (27%), Positives = 112/230 (48%), Gaps = 7/230 (3%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNVR 65
++++P Y+E NIK L + + +EG ++I VDD+S DG+ + VE++ NV
Sbjct: 3 NIIIPTYNEGPNIKVLLRMVSDVMSEEGKPF-KIIVVDDSSPDGTYKTVESMGLP--NVC 59
Query: 66 IIVRTDERGLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLLD-SLRTHPFVLGTRYA 124
++ R + GL SA + + V MD DL H P + ++ + V G+RY
Sbjct: 60 LLSRKKKLGLGSAYKTALEHCEHPFTVVMDGDLSHDPMYIKDMIRLQKKGADIVAGSRY- 118
Query: 125 AGVGIDKDWPLYRRVISSGARAMAKPLTSAS-DPMSGFFGLQKKYLIHKDAKNINPKGFK 183
+G G W + R++IS GA +A+ + + ++G F L + ++ + G+
Sbjct: 119 SGEGAVCGWSMKRKIISLGANNLARIFLNVNVSDLTGSFRLYRTEVLRLLIEESVSTGYS 178
Query: 184 IALDLLVKLPIPPQEPIGEVPFFFGVRTEGESKLSGKVIIQYLHQLRELY 233
++L+ L + E P F R G+SKLS I+ Y+ + L+
Sbjct: 179 FQMELMC-LAKRRGFVVSECPIVFHERRRGQSKLSLMEIVMYIKAIGVLF 227
>YPL227c [R] KOG2977 Glycosyltransferase
Length = 334
Score = 50.1 bits (118), Expect = 3e-06
Identities = 33/112 (29%), Positives = 57/112 (50%), Gaps = 5/112 (4%)
Query: 6 SVVVPAYHEKLNIKPLTTRLFQALGKEGSNITELIFVDDNSQDGSVEEVEALKKQGYNV- 64
SVV+P+Y+E I + T L ++ + E++ VDD S D + + + K+ + +
Sbjct: 76 SVVIPSYNETGRILLMLTDAISFLKEKYGSRWEIVIVDDGSTDNTTQYCLKICKEQFKLN 135
Query: 65 ----RIIVRTDERGLSSAVLKGFHEAKGEYLVCMDADLQHPPESVPQLLDSL 112
RII + RG AV +GF +G+Y + DAD V +L+D++
Sbjct: 136 YEQFRIIKFSQNRGKGGAVRQGFLHIRGKYGLFADADGASKFSDVEKLIDAI 187
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.140 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,834,555
Number of Sequences: 60738
Number of extensions: 681055
Number of successful extensions: 1579
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1559
Number of HSP's gapped (non-prelim): 8
length of query: 265
length of database: 30,389,216
effective HSP length: 104
effective length of query: 161
effective length of database: 24,072,464
effective search space: 3875666704
effective search space used: 3875666704
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)