ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0877 good G KOG3625 Carbohydrate transport and metabolism Alpha amylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0877 309829 311196 456
(456 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR184w [G] KOG3625 Alpha amylase 745 0.0
Hs4557285 [G] KOG3625 Alpha amylase 429 e-120
Hs4557283 [G] KOG3625 Alpha amylase 429 e-120
Hs4557275 [G] KOG3625 Alpha amylase 429 e-120
7291282 [G] KOG3625 Alpha amylase 398 e-110
CE18115 [G] KOG3625 Alpha amylase 365 e-101
>YPR184w [G] KOG3625 Alpha amylase
Length = 1536
Score = 745 bits (1923), Expect = 0.0
Identities = 348/455 (76%), Positives = 388/455 (84%), Gaps = 1/455 (0%)
Query: 1 MRCWGRDVFISLRGLLLATGRFDEAKNHLLAFAKTLKHGLIPNLLDAGRNPRYNARDAAW 60
MRCWGRDVFISLRG+LL TGRFDEAK H+LAFAKTLKHGLIPNLLDAGRNPRYNARDAAW
Sbjct: 1080 MRCWGRDVFISLRGMLLTTGRFDEAKAHILAFAKTLKHGLIPNLLDAGRNPRYNARDAAW 1139
Query: 61 FFVQSVQDYVQTVPNGESILKERVSRRFPKDDRYIQWTDPEAFSYESTIEDILYEIVSRH 120
FF+Q+VQDYV VP+GE IL+E+V+RRFP DD YI DP AFSY ST+E+I+YEI+SRH
Sbjct: 1140 FFLQAVQDYVYIVPDGEKILQEQVTRRFPLDDTYIPVDDPRAFSYSSTLEEIIYEILSRH 1199
Query: 121 AKGIKYREANAGPNLDRVMKDEGFNVEVKVDWTNGLIFGGSQSNCGTWMDKMGESELAGS 180
AKGIK+REANAGPNLDRVM D+GFNVE+ VDW+ GLI GGSQ NCGTWMDKMGESE AGS
Sbjct: 1200 AKGIKFREANAGPNLDRVMTDKGFNVEIHVDWSTGLIHGGSQYNCGTWMDKMGESEKAGS 1259
Query: 181 VGIPGTPRDGAAIEINGLLKSCLRFLSELHEKGLFKYDHVEKPNGEKVSLKEWNDLVLTN 240
VGIPGTPRDGAAIEINGLLKS LRF+ EL KGLFK+ VE +G ++ EWN L+ N
Sbjct: 1260 VGIPGTPRDGAAIEINGLLKSALRFVIELKNKGLFKFSDVETQDGGRIDFTEWNQLLQDN 1319
Query: 241 FEMKFYVPSSEDEDDRYDIDASL-VNRRGIYKDLYRSGKPYEDYQLRPNFAIAMTVAPEL 299
FE ++YVP +D YD+ A L VNRRGIY+DLY+SGKPYEDYQLRPNFAIAMTVAPEL
Sbjct: 1320 FEKRYYVPEDPSQDADYDVSAKLGVNRRGIYRDLYKSGKPYEDYQLRPNFAIAMTVAPEL 1379
Query: 300 FTPDYAYNALNIADEALRGPVGMRTLDPTDYNYRPYYRNSEDSTDFHTAKGRNYHQGPEW 359
F P++A A+ IADE LRGPVGMRTLDP+DYNYRPYY N EDS DF T+KGRNYHQGPEW
Sbjct: 1380 FVPEHAIKAITIADEVLRGPVGMRTLDPSDYNYRPYYNNGEDSDDFATSKGRNYHQGPEW 1439
Query: 360 VWCYGYFLRAYHHFHFLSNPLAQNPSKTLPSSYLYQQLYKRLDGHRKWIRESEWAGLTEL 419
VW YGYFLRA+HHFHF ++P QN +K PSSYLYQQLY RL GHRKWI ES WAGLTEL
Sbjct: 1440 VWLYGYFLRAFHHFHFKTSPRCQNAAKEKPSSYLYQQLYYRLKGHRKWIFESVWAGLTEL 1499
Query: 420 TNKDGDICQDSSPTQAWSTGCLLDFFYDLWDRYED 454
TNKDG++C DSSPTQAWS+ CLLD FYDLWD YED
Sbjct: 1500 TNKDGEVCNDSSPTQAWSSACLLDLFYDLWDAYED 1534
>Hs4557285 [G] KOG3625 Alpha amylase
Length = 1516
Score = 429 bits (1103), Expect = e-120
Identities = 228/450 (50%), Positives = 297/450 (65%), Gaps = 17/450 (3%)
Query: 2 RCWGRDVFISLRGLLLATGRFDEAKNHLLAFAKTLKHGLIPNLLDAGRNPRYNARDAAWF 61
RCWGRD FI+LRG+LL TGR+ EA+N +LAFA TL+HGLIPNLL G RYN RDA W+
Sbjct: 1081 RCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWW 1140
Query: 62 FVQSVQDYVQTVPNGESILKERVSRRFPKDDRYIQWTDPEAFSYESTIEDILYEIVSRHA 121
++Q +QDY + VPNG ILK VSR +P DD A + + + +++ E + +H
Sbjct: 1141 WLQCIQDYCKMVPNGLDILKCPVSRMYPTDDS----APLPAGTLDQPLFEVIQEAMQKHM 1196
Query: 122 KGIKYREANAGPNLDRVMKDEGFNVEVKVDWTNGLIFGGSQSNCGTWMDKMGESELAGSV 181
+GI++RE NAGP +DR MKDEGFN+ VD G ++GG++ NCGTWMDKMGES+ A +
Sbjct: 1197 QGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNR 1256
Query: 182 GIPGTPRDGAAIEINGLLKSCLRFLSELHEKGLFKYDHVE-KPNGE--KVSLKEWNDLVL 238
GIP TPRDG+A+EI GL KS +R+L EL +K +F Y V K +G+ KVS EWN +
Sbjct: 1257 GIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQ 1316
Query: 239 TNFEMKFYVPSSEDEDDRYDIDASLVNRRGIYKDLYRSGKPYEDYQLRPNFAIAMTVAPE 298
NFE F+V SED D + +LV++RGIYKD Y + P+ DYQLRPNF IAM VAPE
Sbjct: 1317 DNFEKLFHV--SEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPE 1374
Query: 299 LFTPDYAYNALNIADEALRGPVGMRTLDPTDYNYRPYYRNSEDSTDFHTAKGRNYHQGPE 358
LFT + A AL IA++ L GP+GM+TLDP D Y Y N+ D+ +++ AKG NYHQGPE
Sbjct: 1375 LFTTEKAGKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPE 1434
Query: 359 WVWCYGYFLRAYHHFHFLSNPLAQNPSKTLPSSYLYQQLYKRLDGHRKWIRESEWAGLTE 418
W+W GYFLRA +F L P + +KT+ L + + R H + S W GL E
Sbjct: 1435 WLWPIGYFLRAKLYFSRLMGP--ETTAKTI---VLVKNVLSR---HYVHLERSPWKGLPE 1486
Query: 419 LTNKDGDICQDSSPTQAWSTGCLLDFFYDL 448
LTN++ C S TQAWS +L+ YDL
Sbjct: 1487 LTNENAQYCPFSCETQAWSIATILETLYDL 1516
>Hs4557283 [G] KOG3625 Alpha amylase
Length = 1515
Score = 429 bits (1103), Expect = e-120
Identities = 228/450 (50%), Positives = 297/450 (65%), Gaps = 17/450 (3%)
Query: 2 RCWGRDVFISLRGLLLATGRFDEAKNHLLAFAKTLKHGLIPNLLDAGRNPRYNARDAAWF 61
RCWGRD FI+LRG+LL TGR+ EA+N +LAFA TL+HGLIPNLL G RYN RDA W+
Sbjct: 1080 RCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWW 1139
Query: 62 FVQSVQDYVQTVPNGESILKERVSRRFPKDDRYIQWTDPEAFSYESTIEDILYEIVSRHA 121
++Q +QDY + VPNG ILK VSR +P DD A + + + +++ E + +H
Sbjct: 1140 WLQCIQDYCKMVPNGLDILKCPVSRMYPTDDS----APLPAGTLDQPLFEVIQEAMQKHM 1195
Query: 122 KGIKYREANAGPNLDRVMKDEGFNVEVKVDWTNGLIFGGSQSNCGTWMDKMGESELAGSV 181
+GI++RE NAGP +DR MKDEGFN+ VD G ++GG++ NCGTWMDKMGES+ A +
Sbjct: 1196 QGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNR 1255
Query: 182 GIPGTPRDGAAIEINGLLKSCLRFLSELHEKGLFKYDHVE-KPNGE--KVSLKEWNDLVL 238
GIP TPRDG+A+EI GL KS +R+L EL +K +F Y V K +G+ KVS EWN +
Sbjct: 1256 GIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQ 1315
Query: 239 TNFEMKFYVPSSEDEDDRYDIDASLVNRRGIYKDLYRSGKPYEDYQLRPNFAIAMTVAPE 298
NFE F+V SED D + +LV++RGIYKD Y + P+ DYQLRPNF IAM VAPE
Sbjct: 1316 DNFEKLFHV--SEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPE 1373
Query: 299 LFTPDYAYNALNIADEALRGPVGMRTLDPTDYNYRPYYRNSEDSTDFHTAKGRNYHQGPE 358
LFT + A AL IA++ L GP+GM+TLDP D Y Y N+ D+ +++ AKG NYHQGPE
Sbjct: 1374 LFTTEKAGKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPE 1433
Query: 359 WVWCYGYFLRAYHHFHFLSNPLAQNPSKTLPSSYLYQQLYKRLDGHRKWIRESEWAGLTE 418
W+W GYFLRA +F L P + +KT+ L + + R H + S W GL E
Sbjct: 1434 WLWPIGYFLRAKLYFSRLMGP--ETTAKTI---VLVKNVLSR---HYVHLERSPWKGLPE 1485
Query: 419 LTNKDGDICQDSSPTQAWSTGCLLDFFYDL 448
LTN++ C S TQAWS +L+ YDL
Sbjct: 1486 LTNENAQYCPFSCETQAWSIATILETLYDL 1515
>Hs4557275 [G] KOG3625 Alpha amylase
Length = 1532
Score = 429 bits (1103), Expect = e-120
Identities = 228/450 (50%), Positives = 297/450 (65%), Gaps = 17/450 (3%)
Query: 2 RCWGRDVFISLRGLLLATGRFDEAKNHLLAFAKTLKHGLIPNLLDAGRNPRYNARDAAWF 61
RCWGRD FI+LRG+LL TGR+ EA+N +LAFA TL+HGLIPNLL G RYN RDA W+
Sbjct: 1097 RCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWW 1156
Query: 62 FVQSVQDYVQTVPNGESILKERVSRRFPKDDRYIQWTDPEAFSYESTIEDILYEIVSRHA 121
++Q +QDY + VPNG ILK VSR +P DD A + + + +++ E + +H
Sbjct: 1157 WLQCIQDYCKMVPNGLDILKCPVSRMYPTDDS----APLPAGTLDQPLFEVIQEAMQKHM 1212
Query: 122 KGIKYREANAGPNLDRVMKDEGFNVEVKVDWTNGLIFGGSQSNCGTWMDKMGESELAGSV 181
+GI++RE NAGP +DR MKDEGFN+ VD G ++GG++ NCGTWMDKMGES+ A +
Sbjct: 1213 QGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNR 1272
Query: 182 GIPGTPRDGAAIEINGLLKSCLRFLSELHEKGLFKYDHVE-KPNGE--KVSLKEWNDLVL 238
GIP TPRDG+A+EI GL KS +R+L EL +K +F Y V K +G+ KVS EWN +
Sbjct: 1273 GIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQ 1332
Query: 239 TNFEMKFYVPSSEDEDDRYDIDASLVNRRGIYKDLYRSGKPYEDYQLRPNFAIAMTVAPE 298
NFE F+V SED D + +LV++RGIYKD Y + P+ DYQLRPNF IAM VAPE
Sbjct: 1333 DNFEKLFHV--SEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPE 1390
Query: 299 LFTPDYAYNALNIADEALRGPVGMRTLDPTDYNYRPYYRNSEDSTDFHTAKGRNYHQGPE 358
LFT + A AL IA++ L GP+GM+TLDP D Y Y N+ D+ +++ AKG NYHQGPE
Sbjct: 1391 LFTTEKAGKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPE 1450
Query: 359 WVWCYGYFLRAYHHFHFLSNPLAQNPSKTLPSSYLYQQLYKRLDGHRKWIRESEWAGLTE 418
W+W GYFLRA +F L P + +KT+ L + + R H + S W GL E
Sbjct: 1451 WLWPIGYFLRAKLYFSRLMGP--ETTAKTI---VLVKNVLSR---HYVHLERSPWKGLPE 1502
Query: 419 LTNKDGDICQDSSPTQAWSTGCLLDFFYDL 448
LTN++ C S TQAWS +L+ YDL
Sbjct: 1503 LTNENAQYCPFSCETQAWSIATILETLYDL 1532
>7291282 [G] KOG3625 Alpha amylase
Length = 1539
Score = 398 bits (1022), Expect = e-110
Identities = 208/452 (46%), Positives = 281/452 (62%), Gaps = 26/452 (5%)
Query: 1 MRCWGRDVFISLRGLLLATGRFDEAKNHLLAFAKTLKHGLIPNLLDAGRNPRYNARDAAW 60
MRCWGRD FI+LRG + TGR++EA+ ++ F +TL+HGLIPNLLD+G PR+N RDA W
Sbjct: 1102 MRCWGRDTFIALRGSMFLTGRYNEARFIIIGFGQTLRHGLIPNLLDSGSKPRFNCRDAIW 1161
Query: 61 FFVQSVQDYVQTVPNGESILKERVSRRFPKDDRYIQWTDPEAFSYESTIEDILYEIVSRH 120
+++ ++ YV+ P G ILK++VSR FP DD P AF + + D++ E + H
Sbjct: 1162 WWMYCIKQYVEDAPKGAEILKDKVSRIFPYDDADAH--APGAF--DQLLFDVMQEALQVH 1217
Query: 121 AKGIKYREANAGPNLDRVMKDEGFNVEVKVDWTNGLIFGGSQSNCGTWMDKMGESELAGS 180
+G++YRE NAG +D M D+GFN ++ + G +FGG+ NCGTWMDKMG S+ AG+
Sbjct: 1218 FQGLQYRERNAGYEIDAHMVDQGFNNQIGIHPETGFVFGGNNFNCGTWMDKMGSSQKAGN 1277
Query: 181 VGIPGTPRDGAAIEINGLLKSCLRFLSELHEKGLFKYDHVEK--PNGE--KVSLKEWNDL 236
G P TPRDG+A+E+ GL + LRF+ L EK + Y VE+ P+GE K S KEW D
Sbjct: 1278 KGRPSTPRDGSAVELVGLQYAVLRFMQSLAEKEVIPYTGVERKGPSGEVTKWSYKEWADR 1337
Query: 237 VLTNFEMKFYVPSSEDEDDRYDIDASLVNRRGIYKDLYRSGKPYEDYQLRPNFAIAMTVA 296
+ NF+ F+V SE S+ N++ IYKD Y + + + DYQLR NF I +TVA
Sbjct: 1338 IKNNFDKYFFVSESET--------CSVANKKLIYKDSYGATQSWTDYQLRCNFPITLTVA 1389
Query: 297 PELFTPDYAYNALNIADEALRGPVGMRTLDPTDYNYRPYYRNSEDSTDFHTAKGRNYHQG 356
P+L P A+ AL A + L GP+GM+T+DP D+NYR Y NS DSTD A G NYHQG
Sbjct: 1390 PDLCNPQNAWRALERAKKYLLGPLGMKTMDPEDWNYRANYDNSNDSTDCTVAHGANYHQG 1449
Query: 357 PEWVWCYGYFLRAYHHFHFLSNPLAQNPSKTLPSSYLYQQLYKRLDGHRKWIRESEWAGL 416
PEWVW G++LRA F L + ++T + L H + ++ S W GL
Sbjct: 1450 PEWVWPIGFYLRARLIFAKKCGHLDETIAET----------WAILRAHLRELQTSHWRGL 1499
Query: 417 TELTNKDGDICQDSSPTQAWSTGCLLDFFYDL 448
ELTN +G C DS TQAWS +L+ YDL
Sbjct: 1500 PELTNDNGSYCGDSCRTQAWSVAAILEVLYDL 1531
>CE18115 [G] KOG3625 Alpha amylase
Length = 1467
Score = 365 bits (938), Expect = e-101
Identities = 200/442 (45%), Positives = 262/442 (59%), Gaps = 26/442 (5%)
Query: 2 RCWGRDVFISLRGLLLATGRFDEAKNHLLAFAKTLKHGLIPNLLDAGRNPRYNARDAAWF 61
R WGRD FI+L G LL+TGRF EA+ +L+FA ++HGLIPNLL G RYN RDA WF
Sbjct: 1042 RNWGRDTFIALPGCLLSTGRFQEARQIILSFAGAIRHGLIPNLLAEGIGARYNCRDATWF 1101
Query: 62 FVQSVQDYVQTVPNGESILKERVSRRFPKDDRYIQWTDPEAFSYESTIEDILYEIVSRHA 121
++ S+ YV++ PNG IL++ V R +P DD + + E+ +YE + +H
Sbjct: 1102 WLVSIVKYVESAPNGVGILEDPVRRIYPNDDSVYGEGEVQQMLIET-----IYEALDKHF 1156
Query: 122 KGIKYREANAGPNLDRVMKDEGFNVEVKVDWTNGLIFGGSQSNCGTWMDKMGESELAGSV 181
GI YRE +AGP +D M+DEGF V V T G I+GG++ NCGTWMDKMG SE AG+
Sbjct: 1157 AGIDYRERSAGPQIDEQMRDEGFQVTAGVSRTTGFIYGGNRWNCGTWMDKMGSSERAGNK 1216
Query: 182 GIPGTPRDGAAIEINGLLKSCLRFLSELHEKGLFKYDHVEKPNGEKVSLKEWNDLVLTNF 241
G P TPRDGAA+E+ GL L+ L E+G+ + V ++ + W + NF
Sbjct: 1217 GEPATPRDGAAVELQGLAYRALKSLKNWKEQGVIQRSGV----SDEWTWGFWAQKIRENF 1272
Query: 242 EMKFYVPSSEDEDDRYDIDASLVNRRGIYKDLYRSGKPYEDYQLRPNFAIAMTVAPELFT 301
E +F+V + D Y A VNRR I KD S + D+QLR NF IA+ VAP+L
Sbjct: 1273 EKEFFV-----DKDSY---AEFVNRREIIKDSVGSTLGFTDFQLRCNFGIALAVAPDLMD 1324
Query: 302 PDYAYNALNIADEALRGPVGMRTLDPTDYNYRPYYRNSEDSTDFHTAKGRNYHQGPEWVW 361
P A+ AL+ A E L GP+GM+TLDPTD+ Y YY N +D TD TAKG NYHQGPEW++
Sbjct: 1325 PKKAWKALDSA-EVLLGPLGMKTLDPTDWAYNGYYNNDDDGTDKKTAKGWNYHQGPEWLF 1383
Query: 362 CYGYFLRAYHHFHFLSNPLAQNPSKTLPSSYLYQQLYKRLDGHRKWIRESEWAGLTELTN 421
GY+L+A + K Y +Q+ +RL K I S W L ELTN
Sbjct: 1384 VAGYYLQA----RLRIGDILGGSEK----QYAIRQVQERLGNAYKHIISSPWRSLPELTN 1435
Query: 422 KDGDICQDSSPTQAWSTGCLLD 443
DG+ C S QAWS GCL++
Sbjct: 1436 ADGEYCMQSCAAQAWSVGCLIE 1457
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,486,520
Number of Sequences: 60738
Number of extensions: 1460771
Number of successful extensions: 3471
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3443
Number of HSP's gapped (non-prelim): 7
length of query: 456
length of database: 30,389,216
effective HSP length: 110
effective length of query: 346
effective length of database: 23,708,036
effective search space: 8202980456
effective search space used: 8202980456
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)