ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactII0887   suspect: LH   U     KOG4573  Intracellular trafficking, secretion, and vesicular transport  Phosphoprotein involved in cytoplasm to vacuole targeting and autophagy

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactII0887  311810  313861 684  
         (684 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YPR185w [U] KOG4573 Phosphoprotein involved in cytoplasm to vacu...   219   1e-56
SPAC4F10.07c [U] KOG4573 Phosphoprotein involved in cytoplasm to...    92   2e-18

>YPR185w [U] KOG4573 Phosphoprotein involved in cytoplasm to vacuole
           targeting and autophagy
          Length = 738

 Score =  219 bits (558), Expect = 1e-56
 Identities = 123/264 (46%), Positives = 170/264 (63%), Gaps = 18/264 (6%)

Query: 5   EVQVIELINNFFLKSALLLEQSKVA-HNFDTEEALRDGNHLFNIETRGDPLLEAQIQPWI 63
           E QV++LI++FFLK+ LL+  ++ + +   TE      +  F   +     L   I  W 
Sbjct: 8   EKQVLQLIDSFFLKTTLLICSTESSRYQSSTENIFLFDDTWFEDHSELVSELPEIISKWS 67

Query: 64  TFDGVKTMPPLVIETYLDLRALQPNHMVYLHDADGNPWMVCKGGKKTEIVLERWLVELDK 123
            +DG K +PPLV+ETYLDLR L  +H+V L D +G+ W VCKG KK EIV+ERWL+ELD 
Sbjct: 68  HYDGRKELPPLVVETYLDLRQLNSSHLVRLKDHEGHLWNVCKGTKKQEIVMERWLIELDN 127

Query: 124 QTI---DDSIDSNDPENLHKQLVLLFRYLYTLTQLLPANDI---ITKPHNSQQPA----- 172
            +      S D  D   L KQLVLLFRYL TL QLLP  ++   + K +N  Q       
Sbjct: 128 SSPTFKSYSEDETDVNELSKQLVLLFRYLLTLIQLLPTTELYQLLIKSYNGPQNEGSSNP 187

Query: 173 ------LINVQTRLLDGSKPILSKGRVGLSKPIIASYSNTMNETNIASHLEQRKITPIKT 226
                 L++++T +LDGSKPILSKGR+GLSKPII +YSN +NE+N+ +HL+Q+KITP+ T
Sbjct: 188 ITSTGPLVSIRTCVLDGSKPILSKGRIGLSKPIINTYSNALNESNLPAHLDQKKITPVWT 247

Query: 227 TFGSLRITVSYRKDVDFYVIDSDD 250
            FG LR++VSYR+D  F + +++D
Sbjct: 248 KFGLLRVSVSYRRDWKFEINNTND 271



 Score =  118 bits (295), Expect = 3e-26
 Identities = 92/310 (29%), Positives = 155/310 (49%), Gaps = 47/310 (15%)

Query: 401 DRTAKPRKSTENPPE---DLLEFVKLLEDKKELNMKPNT--ILPQQDISNSLMRFQSMKS 455
           ++ +K  KS   P E   DL++FVKLLE+K +L +K  +    P  +IS+SL+R+Q++K 
Sbjct: 446 EKVSKAVKSPLQPQESQEDLMDFVKLLEEKPDLTIKKTSGNNPPNINISDSLIRYQNLKP 505

Query: 456 NNDALSDNLSMSMSIDQPNV--RFGSNSHSPIPSFSP--NYGSIPSRLSQGSR------- 504
           +ND LS++LS+S+S+D  +   R  S+SHSP+PS SP  +YGS+ SR+SQG+        
Sbjct: 506 SNDLLSEDLSVSLSMDPNHTYHRGRSDSHSPLPSISPSMHYGSLNSRMSQGANASHLIAR 565

Query: 505 --NNSNAELITSRKSSLDRHKHNLLSRTGSNVDINRRGSVGTMETTNEDSKEDEQSHMKG 562
              NS+   + SR++SLD+               N++G  G       +S      + K 
Sbjct: 566 GGGNSSTSALNSRRNSLDKSS-------------NKQGMSGLPPIFGGESTSYHHDN-KI 611

Query: 563 MHFSNEATINKDDDEDEMLMKRSFNAGTSTTEQVMGXXXXXXXXXXXXXXXNQLPLKH-L 621
             ++       DDDE++ L+ +    G S T+                   +++P++   
Sbjct: 612 QKYNQLGVEEDDDDENDRLLNQ---MGNSATKFKSSISPRSIDSISSSFIKSRIPIRQPY 668

Query: 622 NLXXXXXXXXXXXXKFHKSEMSPDPLHTEGAQPHTSSQHHNS--------SQKNDEDDDL 673
           +             KFHK     + L   G + ++++ +HN         + ++D+DDDL
Sbjct: 669 HYSQPTTAPFQAQAKFHK---PANKLIDNGNRSNSNNNNHNGNDAVGVMHNDEDDQDDDL 725

Query: 674 LFVMSDMNLT 683
           +F MSDMNL+
Sbjct: 726 VFFMSDMNLS 735


>SPAC4F10.07c [U] KOG4573 Phosphoprotein involved in cytoplasm to vacuole
           targeting and autophagy
          Length = 758

 Score = 92.4 bits (228), Expect = 2e-18
 Identities = 71/246 (28%), Positives = 120/246 (47%), Gaps = 18/246 (7%)

Query: 10  ELINNFFLKSALLLEQSKVAHNFDTEEALRDG--NHLFNIETRGDPLLEAQIQPW--ITF 65
           ++I++ F K+ L++ +S++ + F T         N  FN+E     L   Q + W  I  
Sbjct: 42  QVIHHCFYKTGLIILESRL-NVFGTSRPRESSKNNKWFNLEIVETELYAEQFKIWKNIEL 100

Query: 66  DGVKTMPPLVIETYLDLRALQPNHMVYLHDADGNPWMVCKGGKKTEIVLERWLVELDKQT 125
              + +PP+V+ TYLD+  L  N  + + D   +  +        +IVLERW+V LD + 
Sbjct: 101 SPSRKIPPMVLHTYLDISDLSKNQTLSVSDGTHSHAINFNNMSTMKIVLERWIVNLDGEA 160

Query: 126 IDDSIDSNDPENLHKQLVLLFRYLYTLTQLLPANDIITKPHNSQQPALINVQTRLLDGSK 185
           +   ++      L+K+LV+LFR LYT T L+P   + +K H  +     +++      + 
Sbjct: 161 LSTPLEL---AVLYKKLVVLFRSLYTYTHLMPLWKLKSKIHKLRAHGT-SLKVGCALSTD 216

Query: 186 PILSKGRVGLSKPIIASYSNTMNETNIASHLEQRKITPIKTTFGSLRITVSYRKDVDFYV 245
            +LS   + +S PI +S         + S +     +P+ T  G  RI+V YRK+  F V
Sbjct: 217 DVLSNDFLPISAPISSS---------LGSSIATFSFSPVGTPAGDFRISVQYRKNCHFEV 267

Query: 246 IDSDDL 251
            DSD L
Sbjct: 268 HDSDAL 273


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.312    0.129    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,795,539
Number of Sequences: 60738
Number of extensions: 1441410
Number of successful extensions: 4294
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4280
Number of HSP's gapped (non-prelim): 4
length of query: 684
length of database: 30,389,216
effective HSP length: 113
effective length of query: 571
effective length of database: 23,525,822
effective search space: 13433244362
effective search space used: 13433244362
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)