ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0981 good F KOG4313 Nucleotide transport and metabolism Thiamine pyrophosphokinase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0981 342914 341889 -342
(342 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR142w [F] KOG4313 Thiamine pyrophosphokinase 401 e-112
SPAC6F12.05c_1 [F] KOG4313 Thiamine pyrophosphokinase 177 2e-44
At5g19460 [F] KOG4313 Thiamine pyrophosphokinase 139 8e-33
At5g19470 [F] KOG4313 Thiamine pyrophosphokinase 123 3e-28
7288992 [F] KOG4313 Thiamine pyrophosphokinase 108 9e-24
>YJR142w [F] KOG4313 Thiamine pyrophosphokinase
Length = 342
Score = 401 bits (1031), Expect = e-112
Identities = 181/336 (53%), Positives = 252/336 (74%)
Query: 6 STAGREVLIRSEKDKLNNFSFLPIIDKVDVFPRNYANNEHFQKNVYMLKSHTGVDIGFVL 65
S+ G EVL+R+++D L FSFL I+D+VD P ++ N ++F++ +Y + +H G IGFVL
Sbjct: 6 SSKGLEVLVRTQEDDLEGFSFLEIMDRVDPLPLDFENYKNFKEGIYYMCTHDGTKIGFVL 65
Query: 66 KLIVLEIREVCGDLATRLFTVNDDSMYVRFKPTSFQERDDLTEELCQLLRVKSKLECIKT 125
K + E+ VC ++ F +++ +RFK F R++L ++L + + ++S L +K
Sbjct: 66 KFAINEMETVCSEIFEETFQLDESRHELRFKSEDFDHRNNLIDQLARKMYLESSLSGVKG 125
Query: 126 WRDEKYAVYVEHEPYVLIERGLAGAFGIVTYGVHVNGFFRDSTSGEIKFWIPRRSATKPT 185
WR+EKYAV+V +PYVL+ER +AG GI+TYG+H+NG+ D S +++FW+PRRS TK T
Sbjct: 126 WRNEKYAVWVNKKPYVLVERAVAGVLGIITYGIHINGYVLDPKSKKVQFWVPRRSKTKQT 185
Query: 186 WPSMLDNIVAGGIGHPHGIYETVLKECMEEATLSADVIEKNIKSVGSVSYLFFQGDIEEE 245
WP MLDNI+AGG+G+P+GIYETVLKE MEEA L VIE NIK+ GSVSYL+F GDI
Sbjct: 186 WPLMLDNIIAGGLGYPYGIYETVLKESMEEANLEKSVIEDNIKATGSVSYLYFTGDISVT 245
Query: 246 RFEHESAFITGEVEYIFDVELPPDVIPVPNDGEVEQFGIFGLQEVVDALKRGEFKPNCAL 305
+F ES FI GEV+Y++D++L D+IP PNDGEVE F +F LQE ++AL++ EFKPNCAL
Sbjct: 246 KFNKESDFIVGEVQYVYDLKLSEDIIPKPNDGEVESFNLFSLQETINALRKKEFKPNCAL 305
Query: 306 IMVEFLIRHGYITPEIEPNYTEIISRMHRQLPFPVM 341
+MV+FLIRHGYITPE EPNY E+++RMHR+LPFP +
Sbjct: 306 VMVDFLIRHGYITPENEPNYLELVTRMHRRLPFPTL 341
>SPAC6F12.05c_1 [F] KOG4313 Thiamine pyrophosphokinase
Length = 336
Score = 177 bits (450), Expect = 2e-44
Identities = 105/284 (36%), Positives = 157/284 (54%), Gaps = 17/284 (5%)
Query: 58 GVDIGFVLKLIVLEIREVCGDLATRLFTVNDDSMYVRFKPTSFQERDDLTEELCQLLRVK 117
G IG+V L VLEI + V + Y+ ++F++R D+ ++ + R
Sbjct: 39 GAAIGYVTPL-VLEILIKADNFKFNWVYVPGE--YIEINASTFEKRTDILAKVLEHWRHN 95
Query: 118 SKLECIKTWRDEKYAVYVEHE-PYVLIERGLAGAFGIVTYGVHVNGFFRDSTSGEIKFWI 176
+ WR+E Y VY + + P + +ERG FG ++ GVH + + ++ W+
Sbjct: 96 NTFGIADQWRNELYTVYGKSKKPVLAVERGGFWLFGFLSTGVHCTMYIPATKEHPLRIWV 155
Query: 177 PRRSATKPTWPSMLDNIVAGGIGHPHGIYETVLKECMEEATLSADVIEKNIKSVGSVSYL 236
PRRS TK TWP+ LDN VAGGI H + T++KE EEA L DV N+ G+VSY+
Sbjct: 156 PRRSPTKQTWPNYLDNSVAGGIAHGDSVIGTMIKEFSEEANL--DVSSMNLIPCGTVSYI 213
Query: 237 FFQGDIEEERFEHESAFITGEVEYIFDVELPPDVIPVPNDGEVEQFGIFGLQEVVDALKR 296
+ + +I E++Y+FD+ + VIP NDGEV F + L +V+ L+
Sbjct: 214 KME----------KRHWIQPELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHELEL 263
Query: 297 GEFKPNCALIMVEFLIRHGYITPEIEPNYTEIISRMHRQLPFPV 340
FKPNCAL++++FLIRHG ITP+ P Y + + R+HR LP PV
Sbjct: 264 KSFKPNCALVLLDFLIRHGIITPQ-HPQYLQTLERIHRPLPVPV 306
>At5g19460 [F] KOG4313 Thiamine pyrophosphokinase
Length = 403
Score = 139 bits (349), Expect = 8e-33
Identities = 84/238 (35%), Positives = 133/238 (55%), Gaps = 18/238 (7%)
Query: 101 QERDDLTEELCQLLRVKSKLECIKTWRDEKYAVYVEHEPYVL--IERGLAGAFGIVTYGV 158
Q+ +D T + ++++ I R+E Y V V +ER A FGI YGV
Sbjct: 178 QKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVFFSLERAAAPYFGIKGYGV 237
Query: 159 HVNGFFRDSTSGEIKFWIPRRSATKPTWPSMLDNIVAGGIGHPHGIYETVLKECMEEATL 218
H+NG+ G+ WI +RS +K T+P MLD++VAGG+ H ++KEC EEA +
Sbjct: 238 HMNGYVE--RDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGISCGGNLVKECEEEAGI 295
Query: 219 SADVIEKNIKSVGSVSYLFFQGDIEEERFEHESAFITGEVEYIFDVELPPDVIPVPNDGE 278
S + ++ I +VG+VSYL DI++ F+ +V + +D+ELP D +P DGE
Sbjct: 296 SRAIADRAI-AVGAVSYL----DIDQYCFKR-------DVLFCYDLELPEDFVPKNQDGE 343
Query: 279 VEQFGIFGLQEVVDALKRGE-FKPNCALIMVEFLIRHGYITPEIEPNYTEIISRMHRQ 335
VE F + + +V +K+ FK NC+L++++FL RHG+I PE Y ++ R+ +
Sbjct: 344 VESFKLIPVAQVASVIKKTSFFKANCSLVIIDFLFRHGFIRPE-SSGYLDLYQRLRNR 400
>At5g19470 [F] KOG4313 Thiamine pyrophosphokinase
Length = 373
Score = 123 bits (309), Expect = 3e-28
Identities = 73/216 (33%), Positives = 120/216 (54%), Gaps = 17/216 (7%)
Query: 104 DDLTEELCQLLRVKSKLECIKTWRDEKYAVYVEHEP--YVLIERGLAGAFGIVTYGVHVN 161
++ T + ++++ I R+E Y V + IER A FG+ Y +HVN
Sbjct: 143 EERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVN 202
Query: 162 GFFRDSTSGEIKFWIPRRSATKPTWPSMLDNIVAGGIGHPHGIYETVLKECMEEATLSAD 221
G+ G+ WI +RS K T+P LD++VAGG+ H + E ++KEC EEA +S
Sbjct: 203 GYVE--RDGQKFLWIGKRSLAKSTYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKV 260
Query: 222 VIEKNIKSVGSVSYLFFQGDIEEERFEHESAFITGEVEYIFDVELPPDVIPVPNDGEVEQ 281
+ ++ I +VG VSY+ DI+ F T +V + +D+ELP D +P DGEV+
Sbjct: 261 LADRAI-AVGVVSYM----DIDRYCF-------TRDVLFCYDLELPQDFVPTNQDGEVDS 308
Query: 282 FGIFGLQEVVDALKRGE-FKPNCALIMVEFLIRHGY 316
F + + +V + +++ FK +C+L++++FL RHGY
Sbjct: 309 FRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGY 344
>7288992 [F] KOG4313 Thiamine pyrophosphokinase
Length = 163
Score = 108 bits (271), Expect = 9e-24
Identities = 60/166 (36%), Positives = 90/166 (54%), Gaps = 13/166 (7%)
Query: 143 IERGLAGAFGIVTYGVHVNGFFRDSTSGEIKFWIPRRSATKPTWPSMLDNIVAGGIGHPH 202
+ER FG+ YGV +NG+ R T G + W+ +RS TK TWP DN+V GG+
Sbjct: 1 MERAATPLFGVRKYGVDINGYVRHPTLG-LCIWLQQRSNTKETWPGKWDNMVGGGLSVGF 59
Query: 203 GIYETVLKECMEEATLSADVIEKNIKSVGSVSYLFFQGDIEEERFEHESAFITGEVEYIF 262
GI ET +KE EEA++ +D++ KN+ S G VS+ F + EY+F
Sbjct: 60 GIKETAIKEAAEEASIPSDLV-KNLVSAGCVSFYF-----------ESRQGLFPNTEYVF 107
Query: 263 DVELPPDVIPVPNDGEVEQFGIFGLQEVVDALKRGEFKPNCALIMV 308
D+ELP D +P DGEV+ F + ++ V+ + +FK A + +
Sbjct: 108 DLELPLDFVPQNADGEVQAFELLTAKDCVERVFTSDFKTTSAPVKI 153
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.141 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,258,691
Number of Sequences: 60738
Number of extensions: 937248
Number of successful extensions: 1992
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1976
Number of HSP's gapped (non-prelim): 5
length of query: 342
length of database: 30,389,216
effective HSP length: 107
effective length of query: 235
effective length of database: 23,890,250
effective search space: 5614208750
effective search space used: 5614208750
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)