ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1002 good E KOG0455 Amino acid transport and metabolism Homoserine dehydrogenase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1002 Similar to YJR139c SP|P31116 HOM6 homoserine
dehydrogenase singleton
(340 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR139c [E] KOG0455 Homoserine dehydrogenase 415 e-116
SPBC776.03 [E] KOG0455 Homoserine dehydrogenase 303 2e-82
At5g21060 [E] KOG0455 Homoserine dehydrogenase 139 5e-33
Hs4505235 [G] KOG2757 Mannose-6-phosphate isomerase 30 5.1
>YJR139c [E] KOG0455 Homoserine dehydrogenase
Length = 359
Score = 415 bits (1066), Expect = e-116
Identities = 215/340 (63%), Positives = 253/340 (74%), Gaps = 1/340 (0%)
Query: 1 MKQLLSIKSQITYNVILVAKYSNALVSKDYSPLKLDASSWEETVNAEAGNPISVPEIFEF 60
+ QLL++KS ITYN++L+A+ +L+SKD+SPL + S W+ + A + + ++
Sbjct: 21 LDQLLAMKSTITYNLVLLAEAERSLISKDFSPLNV-GSDWKAALAASTTKTLPLDDLIAH 79
Query: 61 LQKSPLPTILVDNTSNEEIANFYPKFIESGISIATPNKKAFSSNLELWNNIFSAKPSNGL 120
L+ SP P ILVDNTS+ IA FY KF+E+GISIATPNKKAFSS+L W +FS KP+NG
Sbjct: 80 LKTSPKPVILVDNTSSAYIAGFYTKFVENGISIATPNKKAFSSDLATWKALFSNKPTNGF 139
Query: 121 VYHEATVGAGLPIIGPLRDMIQTGDKVVKIEGIFSGTLSYIFNEFSTSTPNDVGFSSVVK 180
VYHEATVGAGLPII LR++IQTGD+V KIEGIFSGTLSYIFNEFSTS NDV FS VVK
Sbjct: 140 VYHEATVGAGLPIISFLREIIQTGDEVEKIEGIFSGTLSYIFNEFSTSQANDVKFSDVVK 199
Query: 181 VAKELGYTEPDPRDDLNGLDVARKVTILARIAGFKVESPTSFPVQSLIPSALESVKSSDE 240
VAK+LGYTEPDPRDDLNGLDVARKVTI+ RI+G +VESPTSFPVQSLIP LESVKS+DE
Sbjct: 200 VAKKLGYTEPDPRDDLNGLDVARKVTIVGRISGVEVESPTSFPVQSLIPKPLESVKSADE 259
Query: 241 FLQKLPDYDHXXXXXXXXXXXXXXVLRFVGKVDFPSNVVSVGIEKYDYSHPFASLKGSDN 300
FL+KL DYD VLRF+GKVD + VSVGIEKYDYSHPFASLKGSDN
Sbjct: 260 FLEKLSDYDKDLTQLKKEAATENKVLRFIGKVDVATKSVSVGIEKYDYSHPFASLKGSDN 319
Query: 301 VISIQTERYTNPXXXXXXXXXXXXXXXXXXXXXIKIAERL 340
VISI+T+RYTNP IKIA+RL
Sbjct: 320 VISIKTKRYTNPVVIQGAGAGAAVTAAGVLGDVIKIAQRL 359
>SPBC776.03 [E] KOG0455 Homoserine dehydrogenase
Length = 376
Score = 303 bits (776), Expect = 2e-82
Identities = 159/306 (51%), Positives = 210/306 (67%), Gaps = 5/306 (1%)
Query: 6 SIKSQITYNVILVAKYSNALVSKDYSPLKLDASSWEETVNAEAGNPISVPEIFEFLQKSP 65
S ++NV+ ++ VSKDY P+ L S W+ + G S+ + +FL KSP
Sbjct: 34 STNGTTSFNVVAISSMEGHYVSKDYQPINL--SEWKNLTSQNQG-AYSLDALVDFLAKSP 90
Query: 66 LPTILVDNTSNEEIANFYPKFIESGISIATPNKKAFSSNLELWNNIFSA-KPSNGLVYHE 124
LP ILVDNT++E IA YPKF+ I+IATPNKKAFS++ +++ NI A K S L+ HE
Sbjct: 91 LPAILVDNTASEAIAQSYPKFLSKKINIATPNKKAFSASNDVYQNIIKASKESGALLMHE 150
Query: 125 ATVGAGLPIIGPLRDMIQTGDKVVKIEGIFSGTLSYIFNEFSTS-TPNDVGFSSVVKVAK 183
A+VGAGLPII L+++I TGD+++KIEGIFSGTLSYIFN +S + FS +VK+AK
Sbjct: 151 ASVGAGLPIISTLKELIATGDEIIKIEGIFSGTLSYIFNVWSPNGKKGTASFSDIVKIAK 210
Query: 184 ELGYTEPDPRDDLNGLDVARKVTILARIAGFKVESPTSFPVQSLIPSALESVKSSDEFLQ 243
+ GYTEPDPRDDLNG+DVARKVTIL+RIAG VES +SFPV+SLIP L+S +++EFL
Sbjct: 211 QNGYTEPDPRDDLNGMDVARKVTILSRIAGVHVESASSFPVKSLIPEPLKSAVNAEEFLA 270
Query: 244 KLPDYDHXXXXXXXXXXXXXXVLRFVGKVDFPSNVVSVGIEKYDYSHPFASLKGSDNVIS 303
LP++D V+RFVG+ D + V +EKYD SHPFA+L+ SDN+IS
Sbjct: 271 GLPNFDSEFASMREEAEKEGKVVRFVGEADVANKTTLVKLEKYDASHPFANLQSSDNIIS 330
Query: 304 IQTERY 309
T+RY
Sbjct: 331 FTTKRY 336
>At5g21060 [E] KOG0455 Homoserine dehydrogenase
Length = 376
Score = 139 bits (351), Expect = 5e-33
Identities = 96/256 (37%), Positives = 136/256 (52%), Gaps = 13/256 (5%)
Query: 56 EIFEFLQKSPLPTILVDNTSNEEIANFYPKFIESGISIATPNKKAFSSNLELWNNIFSAK 115
EI + L KS +VD +++ E K ++ G I NKK +S LE ++ + +
Sbjct: 99 EIAKLLGKST-GLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKL-ALH 156
Query: 116 PSNGLVYHEATVGAGLPIIGPLRDMIQTGDKVVKIEGIFSGTLSYIFNEFSTSTPNDVGF 175
P + HE+TVGAGLP+I L +I +GD V +I G SGTL Y+ +E P
Sbjct: 157 PR--FIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKP----L 210
Query: 176 SSVVKVAKELGYTEPDPRDDLNGLDVARKVTILARIAGFKVESPTSFPVQSLIPSAL-ES 234
S VV+ AK+LGYTEPDPRDDL G+DVARK ILAR+ G ++ S ++SL P +
Sbjct: 211 SQVVQAAKKLGYTEPDPRDDLGGMDVARKGLILARLLGKRI-IMDSIKIESLYPEEMGPG 269
Query: 235 VKSSDEFLQK-LPDYDHXXXXXXXXXXXXXXVLRFVGKVDFPSNVVSVGIEKYDYSHPFA 293
+ S D+FL + D VLR+V ++ S V VGI + P
Sbjct: 270 LMSVDDFLHNGIVKLDQNIEERVKKASSKGCVLRYVCVIEGSS--VQVGIREVSKDSPLG 327
Query: 294 SLKGSDNVISIQTERY 309
L+GSDN++ I + Y
Sbjct: 328 RLRGSDNIVEIYSRCY 343
>Hs4505235 [G] KOG2757 Mannose-6-phosphate isomerase
Length = 423
Score = 30.0 bits (66), Expect = 5.1
Identities = 23/101 (22%), Positives = 45/101 (43%), Gaps = 19/101 (18%)
Query: 149 KIEGIFSGTLSYIFNEFSTSTPNDVGFSSVVKVAKELGYTEPDPRDDLNGL-DVARKVTI 207
K++ F+G L ++F S TP + ++A++L P D N ++A +T
Sbjct: 85 KVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELAEKLHLQAPQHYPDANHKPEMAIALTP 144
Query: 208 LARIAGFKVESPTSFPVQSLIPSALESVKSSDEFLQKLPDY 248
+ GF+ PV+ ++ FL+K+P++
Sbjct: 145 FQGLCGFR-------PVEEIV-----------TFLKKVPEF 167
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.134 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,967,867
Number of Sequences: 60738
Number of extensions: 731268
Number of successful extensions: 1423
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1412
Number of HSP's gapped (non-prelim): 4
length of query: 340
length of database: 30,389,216
effective HSP length: 107
effective length of query: 233
effective length of database: 23,890,250
effective search space: 5566428250
effective search space used: 5566428250
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)