ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1037 good R KOG3009 General function prediction only Predicted carbohydrate kinase, contains PfkB domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1037 367903 365690 -738
(738 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPBC1861.05 [R] KOG3009 Predicted carbohydrate kinase contains P... 357 4e-98
7296188 [R] KOG3009 Predicted carbohydrate kinase contains PfkB ... 321 3e-87
CE28492 [R] KOG3009 Predicted carbohydrate kinase contains PfkB ... 244 5e-64
At1g50510 [R] KOG3009 Predicted carbohydrate kinase contains Pfk... 215 2e-55
>SPBC1861.05 [R] KOG3009 Predicted carbohydrate kinase contains PfkB domain
Length = 747
Score = 357 bits (916), Expect = 4e-98
Identities = 252/746 (33%), Positives = 396/746 (52%), Gaps = 69/746 (9%)
Query: 20 LSVSDEIREALNAKMPVVALESTIISHGLPYPENIEMALKVEQEIRNNGAIPATTAFING 79
L++S E+ EAL +PVVALESTII+HG+PYP+N E+A++VE ++R+ GA+PAT A +NG
Sbjct: 41 LALSKEVTEALKNNVPVVALESTIITHGMPYPQNEELAIQVESKVRSMGAVPATIALLNG 100
Query: 80 IPKVGLKTREIEQLAEYARKG-LVNKVSRRDISYTIAQKLYGGTTISSTMILSHKAGIEV 138
+GL E QL+E A+ G KVSRRD+SY +Q+L GGTT+++TMIL+ AGI+V
Sbjct: 101 QCTIGL---EQFQLSELAKSGETAYKVSRRDLSYVASQRLNGGTTVAATMILARAAGIDV 157
Query: 139 FATXXXXXXXXXXELTLDVSADLEELGRTPVSVVCAGPKAILDIPRTMEYLETKGCMVAT 198
FAT E ++D+SADL ELGRT V+VV AG K+ILDI RT+E LET+G V T
Sbjct: 158 FATGGIGGVHRGAENSMDISADLIELGRTRVAVVSAGVKSILDIGRTLEVLETQGVPVVT 217
Query: 199 MGPPGTNVPGFYTRDSGVKSPYNFNSYLEAAQIIRDGNEFGLNSGYLFCCPPPREVALDD 258
+GPP + P F++R+S +SP + + A ++ + G G L P P ++D
Sbjct: 218 LGPPKSAFPAFFSRESKFQSPLSLETPQLIANMLFSNIQLGQECGTLVAIPTPHHCSIDY 277
Query: 259 NFINDIIEQACQNAAAAGISGKELTPFLLREIASKTSGASVKCNISFVLNNCKIAANIAA 318
+ +IE Q + GI+GK +TP+LL E+ ++ G S+ NI VLNN + A+ IA
Sbjct: 278 EKMEALIETCLQRSVQLGITGKNVTPWLLGELLRESKGKSLNTNIDLVLNNAEKASLIAK 337
Query: 319 SLSKLKQNIIPRYEPKNQRGTMTSQE---------------TSGNEKETKVLS-----AV 358
L+ LK+ + P N T ++ +S +K T+ S
Sbjct: 338 ELAVLKEK--SSFFPTNTGNTFETKPVKQDFFYGKVSDKGVSSSKKKITETTSKPAEVVC 395
Query: 359 IGSVALDTQCTFNS--SHIYQGDSNPGKASTSIGGVGHNVALSANYSNRSSQHQTTLISA 416
+GSV++D+ ++ + + G S+P + + GGV HN+AL+++ S T L+S
Sbjct: 396 VGSVSIDSVLKLDNPLTSKFLGTSHPCHSEQAFGGVAHNMALASSLMGAS----TKLVSC 451
Query: 417 VG-KDIAGKHILSGLGMSADGIYINESER--TAQYISIHSRDGELYIAGSDMDIVTQLPI 473
VG K + I L S+ I E + Y +I+ +G L +AG+DM I+ L
Sbjct: 452 VGTKSVPTSSIKEYLTKSSLQHTIVEKSNFTSCSYTAINDCNGNLLLAGADMAIMENLSY 511
Query: 474 KHITNKLKSLKPNVVLVDANVSVEVLEHLITLREKMGFKLIFEPTSECKAQKIAQAKNLK 533
I + L K + D N+S ++ + T + +++FEPTS K KI LK
Sbjct: 512 SEIKDDLNDAK--YICFDGNISPSLMLDITTSKSSKQ-RVVFEPTSGPKTLKI-----LK 563
Query: 534 VYPNHGFDLITPTVTELKGIYEALDKNNKFDLDNWFPVLDSFKIDRNL--SVNKKASTGE 591
V D ITP EL +++A+ N F+ ++W+ L+SF I + + + +
Sbjct: 564 VLSVASIDFITPNKFELDVLFQAMKDGNFFENESWWQKLNSFGITSSFYNEIERFTKSTG 623
Query: 592 LKYLLDNGIFQTASFLLPYFPRMVIKHGSKGIYIFSMEKDTDPATLELISKYSDYSMYCT 651
++ + +NGI Q LLP+ +++K G G + S EK L+ ++ S S++
Sbjct: 624 IEEITENGILQKCFHLLPFIKNIIVKLGPNGALLISSEK------LQGVNSNS-ASLFTP 676
Query: 652 GEHVGGQQQGVLVEHHAVPAGNTTVTSVTGAGDTLVGILLNEISIDPEFLELKDPARRKH 711
G V V+++ VP T + +G GDT +G +S + + D
Sbjct: 677 G--------NVTVKYYPVPKVIATPVNASGTGDTFIGTFTALLS---KGWGMDD------ 719
Query: 712 RLDRAQYGARITLEDKETISKNLKEL 737
+D AQ A +TL+ +++ +K L
Sbjct: 720 AIDTAQKAAGLTLQCNFSVNPEIKTL 745
>7296188 [R] KOG3009 Predicted carbohydrate kinase contains PfkB domain
Length = 700
Score = 321 bits (822), Expect = 3e-87
Identities = 207/556 (37%), Positives = 301/556 (53%), Gaps = 35/556 (6%)
Query: 10 VQRIGHRRL-HLSVSDEIREALNAKMPVVALESTIISHGLPYPENIEMALKVEQEIRNNG 68
V+R HR+ H+ + EIR+AL + PVVALESTII+HG+P PEN+ AL VE+++R NG
Sbjct: 17 VRRSLHRKHDHIVLHPEIRQALQLQKPVVALESTIITHGMPMPENVVTALAVEEQVRQNG 76
Query: 69 AIPATTAFINGIPKVGLKTREIEQLAEYARKGLVNKVSRRDISYTIAQKLYGGTTISSTM 128
AIPAT ++G KVGL E+ LAE R ++ K SRRD+ + ++++ GGTT+++TM
Sbjct: 77 AIPATIGILDGRIKVGLTREELTSLAEKPRDQVI-KCSRRDLPFVVSRRQSGGTTVAATM 135
Query: 129 ILSHKAGIEVFATXXXXXXXXXXELTLDVSADLEELGRTPVSVVCAGPKAILDIPRTMEY 188
I++H+ GI VFAT ++DVSADL ELGRTPV+VVC+G K+ILDIPRT+E+
Sbjct: 136 IIAHRVGIHVFATGGIGGVHRDGHESMDVSADLTELGRTPVAVVCSGVKSILDIPRTLEF 195
Query: 189 LETKGCMVATMGPPGTNVPGFYTRDSGVKSPYNFNSYLEAAQIIRDGNEFGLNSGYLFCC 248
LET+G VA+ PG P FYTRDSG PYN S EAA+++R E + SG +
Sbjct: 196 LETQGVCVASFDSPGGVFPDFYTRDSGCTVPYNLKSAQEAAELLRSWRELKMESGLVIGV 255
Query: 249 PPPREVALDDNFINDIIEQACQNAAAAGISGKELTPFLLREIASKTSGASVKCNISFVLN 308
P P E A D I + I++A A A GISGKE+TPFLL IA T G S+K NI+ + N
Sbjct: 256 PIPEEFAADKFKIEEAIKEATAQARAQGISGKEVTPFLLAAIAKITEGRSLKSNIALIKN 315
Query: 309 NCKIAANIAASLSKLKQNIIPRYEPKNQRGTMTSQETSGNEKETKVLSAVIGSVALDTQC 368
N K+AA IAASL + + S +K V+G+ LD
Sbjct: 316 NAKVAAQIAASLCDVSTKL-----------------ESPVQKAIDKKPLVVGASILDLSF 358
Query: 369 TFNSSH---IYQGDSNPGKASTSIGGVGHNVALSANYSNRSSQHQTTLISAVGKDIAGKH 425
+ G + A + GGVG N+A LISAVG D G+
Sbjct: 359 KVDDQKRDMKLDGATYSAVAKQAAGGVGRNIAEGI----YKLYGDVNLISAVGNDQMGQT 414
Query: 426 ILSGLGMSAD-GIYINESERTAQYISIHSRDGELYIAGSDMDIVTQLPIKHITNKLKSLK 484
+L + + G+ + ++ T+ I + G+ + +M+I + + + + +
Sbjct: 415 LLQMMPKALKRGLIVADNHNTSLCSLIFDKFGDCKLILGNMEIHQSITAETLQAHHQLFR 474
Query: 485 -PNVVLVDANVSVEVLEHLITLREKMGFKLIFEPTSECKAQKIAQAKNLKVYP--NHGFD 541
++++D+N+S + + ++ + + FEPT K K+ P
Sbjct: 475 EAPLIIMDSNISEQAMASILQQAQINKIPVFFEPT-----DMFIAGKPFKLLPELTKNIR 529
Query: 542 LITPTVTELKGIYEAL 557
LI P + ELK I EA+
Sbjct: 530 LIKPNMQELKTITEAI 545
>CE28492 [R] KOG3009 Predicted carbohydrate kinase contains PfkB domain
Length = 608
Score = 244 bits (622), Expect = 5e-64
Identities = 169/507 (33%), Positives = 249/507 (48%), Gaps = 62/507 (12%)
Query: 20 LSVSDEIREALNAKMPVVALESTIISHGLPYPENIEMALKVEQEIRNNGAIPATTAFING 79
L +S +++EAL + VVALEST+I+HGLPYP N+ A +EQ++R++G+ PAT A +G
Sbjct: 16 LQISPQVKEALASGEGVVALESTVITHGLPYPHNLSTARSLEQKVRSSGSHPATIALFDG 75
Query: 80 IPKVGLKTREIEQLAEYARKGLVNKVSRRDISYTIAQKLYGGTTISSTMILSHKAGIEVF 139
VGL ++E LA KVS RDI+ T+ +K GGTT++STM ++H AGI VF
Sbjct: 76 KIHVGLDDEKLELLASSQN---AVKVSSRDIAKTLIKKEVGGTTVASTMKIAHAAGISVF 132
Query: 140 ATXXXXXXXXXXELTLDVSADLEELGRTPVSVVCAGPKAILDIPRTMEYLETKGCMVATM 199
AT + T DVSADL+EL +TPV VVC+G K+ILDIP+T+EYLET
Sbjct: 133 ATGGIGGVHRGADQTFDVSADLQELSQTPVCVVCSGVKSILDIPKTVEYLETHSVNCIVY 192
Query: 200 GPPGTNVPGFYTRDSGVKSPYNFNSYLEAAQIIRDGNEFGLNSGYLFCCPPPREVALDDN 259
G P F+TR S K+ + S E +++ GL G + CP P + A D +
Sbjct: 193 GQENV-FPSFFTRTSDRKAQFCTESLEEVVHLLKTSKSLGLPYGTILACPIPEKYAADGD 251
Query: 260 FINDIIEQACQNAAAAGISGKELTPFLLREIASKTSGASVKCNISFVLNNCKIAANIAAS 319
I I+QA Q A I+ + +TPF+L + T GAS+ NI+ + NN IA +AA
Sbjct: 252 AIQKAIDQAVQEAIEQNIASQSVTPFILARVNELTQGASMATNIALLENNASIAGRLAAK 311
Query: 320 LSKLKQNIIPRYEPKNQRGTMTSQETSGNEKETKVLSAVIGSVALDTQCTFNSSHIYQGD 379
L + I + SQ T+ ++ KV+S IG+ +D + + G
Sbjct: 312 LCDRRPLTISQ-----------SQPTASTTRKPKVVS--IGATIVDFEAITSEDVKDDGG 358
Query: 380 SNPGKASTSIGGVGHNVALSANYSNRSSQHQTTLISAVGKDIAGKHILSGLGMSADGIYI 439
S G+ +GGV N A + S ISA+G D G
Sbjct: 359 SYNGQVVQRMGGVARNHADALARLGCDS----VFISAIGDDNNGHFF------------- 401
Query: 440 NESERTAQYISIHSRDGELYIAGSDMDIVTQLPIKHITNKLKSLKPNVVLVDANVSVEVL 499
R I S+ D + + +L+D+N+SV V+
Sbjct: 402 --------------RQNSHKIVESNEDALE--------------SSDFILLDSNLSVPVM 433
Query: 500 EHLITLREKMGFKLIFEPTSECKAQKI 526
++ + +K ++ EPT K +K+
Sbjct: 434 ARVLEIAKKHDKQVWLEPTDIDKVKKV 460
>At1g50510 [R] KOG3009 Predicted carbohydrate kinase contains PfkB domain
Length = 362
Score = 215 bits (548), Expect = 2e-55
Identities = 125/335 (37%), Positives = 181/335 (53%), Gaps = 35/335 (10%)
Query: 20 LSVSDEIREALNAKMPVVALESTIISHGLPYPENIEMALKVEQEIRNNGAIPATTAFING 79
+ +S ++ EAL+ VVALESTIISHG+PYP+N++ A +VE +R NGAIPAT A +NG
Sbjct: 31 VKISPQVSEALSNGRAVVALESTIISHGMPYPQNLQTAKEVESIVRENGAIPATIAILNG 90
Query: 80 IPKVGLKTREIEQLAEYARKGLVNKVSRRDISYTIAQKLYGGTTISSTMILSHKAGIEVF 139
+P +GL E+E+LA + V K + RDI+ +A + G TT+S+T+ + GI+VF
Sbjct: 91 VPCIGLSEEELERLASLGKS--VQKTAGRDIANVVATRGNGATTVSATLFFASMVGIQVF 148
Query: 140 ATXXXXXXXXXXELTLDVSADLEELGRTPVSVVCAGPKAILDIPRTMEYLETKGCMVATM 199
T ++D+S+DL LGRTP++V+ AG K+ILDIP+T+EYLET+ VA
Sbjct: 149 VTGGIGGVHRHANHSMDISSDLTALGRTPIAVISAGVKSILDIPKTLEYLETQEVYVAAY 208
Query: 200 GPPGTNVPGFYTRDSGVKSPYNFNS------------------------------YLEAA 229
P F+T SG K+P NS +LE
Sbjct: 209 --KSDEFPAFFTEKSGCKAPSRVNSPEDCARVIGKCSLSLKISSFKTYLCTLIPYHLEIK 266
Query: 230 QIIRDGN-EFGLNSGYLFCCPPPREVALDDNFINDIIEQACQNAAAAGISGKELTPFLLR 288
++ D N + +G LF P P+ + N I ++A A ++G TPFLL
Sbjct: 267 PLLPDANMKLNRQAGILFAIPIPKHHSAAGNLIESATQRALTEAREQNVTGNAETPFLLA 326
Query: 289 EIASKTSGASVKCNISFVLNNCKIAANIAASLSKL 323
+ T G S+ NI+ V NN I + IA +LS+L
Sbjct: 327 RVNELTGGTSLAANIALVKNNALIGSQIAVALSQL 361
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,607,565
Number of Sequences: 60738
Number of extensions: 1831453
Number of successful extensions: 4739
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4720
Number of HSP's gapped (non-prelim): 6
length of query: 738
length of database: 30,389,216
effective HSP length: 114
effective length of query: 624
effective length of database: 23,465,084
effective search space: 14642212416
effective search space used: 14642212416
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)