ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1118 good R KOG2497 General function prediction only Predicted methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1118 395811 396734 308
(308 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR129c [R] KOG2497 Predicted methyltransferase 161 1e-39
SPAC3A11.03 [R] KOG2497 Predicted methyltransferase 78 2e-14
At5g27400 [R] KOG2497 Predicted methyltransferase 78 2e-14
Hs20562158 [R] KOG2497 Predicted methyltransferase 77 4e-14
7293566 [R] KOG2497 Predicted methyltransferase 75 9e-14
CE00292 [R] KOG2497 Predicted methyltransferase 67 2e-11
Hs22046321 [R] KOG2497 Predicted methyltransferase 54 2e-07
YBR271w [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 52 1e-06
Hs22044526 [R] KOG2497 Predicted methyltransferase 52 1e-06
>YJR129c [R] KOG2497 Predicted methyltransferase
Length = 339
Score = 161 bits (408), Expect = 1e-39
Identities = 118/340 (34%), Positives = 174/340 (50%), Gaps = 46/340 (13%)
Query: 4 YDRIHARQPVHEVVRFI--SDNNIEFDEDEFLQQLDIVVHRNQYYAKQLLKQMIQH---- 57
YDR+H R P ++ + S +N F+ ++++V N YY K ++K ++ H
Sbjct: 7 YDRLHQRCPGKYLLEELETSKSNDVLHASRFVCEMELVQKTNAYYCKTIVKMLLDHEWIF 66
Query: 58 -----FESRGSDFVET---MYEPYINLLPVGAPDPQDSDIIQYRFDR-YKVVIEETPSLI 108
+ G D +E +YE YI LL G PDP D+++YRFD K+ IEETP+LI
Sbjct: 67 AKAFTIVNDGEDEIEIYDYLYEKYIKLLSTGKPDPMMKDVVRYRFDEDVKITIEETPNLI 126
Query: 109 CAQGTTGFRTWEAAL----FLCHYMTQHPGLFVTHDS-----LMLELGCGTGIIS--ILY 157
A TTGFRTWEAAL FL H Q D +LE+G GTGI+S IL
Sbjct: 127 SAASTTGFRTWEAALYMGDFLIHKPLQELAPVQGQDDGKKKLNVLEVGAGTGIVSLVILQ 186
Query: 158 KMIKDSQGDCKAGTIIVTDGDSNLLQ-QVSTNFQLNGSLSNDGDVNIGFQRLRWNEDELS 216
K + + VTDGDSNL++ Q+ NF+LN + + + +I QRL W D +
Sbjct: 187 KYHE------FVNKMYVTDGDSNLVETQLKRNFELNNEV-RENEPDIKLQRLWWGSDRVP 239
Query: 217 NYNEIDLILAADVTYDTSVIPDLVKCLSQFKGAHG----YISCTERNLDTLDAFENELTR 272
+IDL++ ADVTYD +++PDL +CL++ +S T R+ T+ F E +
Sbjct: 240 --EDIDLVVGADVTYDPTILPDLCECLAECLALDRCKLCLLSATIRSESTVQLFSQECNK 297
Query: 273 NCIYFEIVSRI------SPISFKQISERDISTSIRIYKIS 306
+ IV+ + K + + + IRIYKI+
Sbjct: 298 LGLKCTIVTSTEYDANNEIRAMKALQFKPLIAPIRIYKIT 337
>SPAC3A11.03 [R] KOG2497 Predicted methyltransferase
Length = 247
Score = 77.8 bits (190), Expect = 2e-14
Identities = 53/148 (35%), Positives = 78/148 (51%), Gaps = 27/148 (18%)
Query: 90 IQYRFDRYKVVIEETPSLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCG 149
+QY Y ++I E+ ++ +GTTG RTWEA + L Y+ QHP V +LELG G
Sbjct: 124 VQYSLGEYDILIRESRHVLLREGTTGARTWEAGMALAEYIYQHP---VQSGMRVLELGAG 180
Query: 150 TGIISILYKMIKDSQGDCKAGTIIV-TDGDSNLLQQVSTNFQLNGSLSNDGDVNIGFQRL 208
TG++SIL K G+I++ TDGD+ + V N +LN D+N+ ++L
Sbjct: 181 TGLVSILC---------AKMGSIVLATDGDTKVCDGVRENARLNNC-----DINV--KKL 224
Query: 209 RWNED--ELSNYNEIDLILAADVTYDTS 234
W D E S D++ A+DV S
Sbjct: 225 LWGVDPPEFS-----DIVFASDVVSQES 247
>At5g27400 [R] KOG2497 Predicted methyltransferase
Length = 369
Score = 77.8 bits (190), Expect = 2e-14
Identities = 87/369 (23%), Positives = 149/369 (39%), Gaps = 76/369 (20%)
Query: 1 MDVYDRIHARQPVHEVV---RFISDNNIEFDEDEFLQQL--DIVVHRNQYYAKQLLKQMI 55
++V A +PV V+ R + +I + F ++ + + N YAK+LLK++I
Sbjct: 12 LNVLSAFLAIEPVDSVITLARGCTGGSITENAQRFFWEICVEAAANGNGSYAKKLLKKLI 71
Query: 56 QHFESRGSDFVETMYEPYINLLPVGAPDP------QDSDIIQYRFDR-----------YK 98
E + ++ +YE Y + D + + I + F K
Sbjct: 72 NEVELENGEVLDEVYEEYALYMLTSKEDILVKENIRITKFISFLFPEGSYKHPSCPRSRK 131
Query: 99 VVIEETPSLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCGTGIISILYK 158
+VI SL +G TG W ++LFL ++ P LF + E+G G G++ I
Sbjct: 132 LVIPLHCSLNMLEGDTGCSIWPSSLFLSEFVLSFPELFA--NKACFEVGSGVGMVGICLA 189
Query: 159 MIKDSQGDCKAGTIIVTDGDSNLLQQVSTNFQLNGSLSND---------GDVNIGFQRLR 209
+ KA +I+TDGD L + N + N +D + L
Sbjct: 190 HV-------KAKEVILTDGDLLTLSNMKLNLERNHLNYDDEFLKQPGEAQSTRVKCTHLP 242
Query: 210 W---NEDELSNYNEIDLILAADVTYDTSVIPDLVKCL----------------------- 243
W +E ELS Y D++L ADV YD S +P L++ L
Sbjct: 243 WETASESELSQYRP-DIVLGADVIYDPSCLPHLLRVLVALLKNPPKRGNGSLETEDRDTT 301
Query: 244 -SQFKGAHGYISCTERNLDTLD-----AFENELTRNCIYFEIVSRISPISFKQISERDIS 297
+ A YI+ RN+DT + A + +L+ + E+ R + + +R
Sbjct: 302 QEEHSPAVAYIASVIRNVDTFNVCLTLADQMDLSITDVTAELTPRFELLPYMHSYDR--- 358
Query: 298 TSIRIYKIS 306
+++R++ IS
Sbjct: 359 STVRLFSIS 367
>Hs20562158 [R] KOG2497 Predicted methyltransferase
Length = 330
Score = 76.6 bits (187), Expect = 4e-14
Identities = 71/255 (27%), Positives = 120/255 (46%), Gaps = 32/255 (12%)
Query: 46 YAKQLLKQMIQHFESRGSDFVETMYEPYINLLPVGAPDPQDSDIIQYRFDRYKVVIEETP 105
YA+ L ++I+ E+ ++ ++ +YE L A + V + E+
Sbjct: 69 YARCFLSELIKKHEAVHTEPLDELYEALAETLM--AKESTQGHRSYLLPSGGSVTLSEST 126
Query: 106 SLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCGTGIISI-LYKMIKDSQ 164
++I + GTTG TW+AAL+L + ++P +F + +LELG G G+ + + KM
Sbjct: 127 AII-SYGTTGLVTWDAALYLAEWAIENPAVFT--NRTVLELGSGAGLTGLAICKM----- 178
Query: 165 GDCKAGTIIVTDGDSNLLQQVSTNFQLNGSLSNDGDV-------NIGFQRLRWN---EDE 214
C+ I +D S +L+Q+ N LNG LS + D+ + +L W+ +
Sbjct: 179 --CRPRAYIFSDCHSRVLEQLRGNVLLNG-LSLEADITAKLDSPRVTVAQLDWDVATVHQ 235
Query: 215 LSNYNEIDLILAADVTYDTSVIPDLV-------KCLSQFKGAHGYISCTERNLDTLDAFE 267
LS + + D+++AADV Y I LV C + Y++ T RN +T F
Sbjct: 236 LSAF-QPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFT 294
Query: 268 NELTRNCIYFEIVSR 282
EL R I +E+ R
Sbjct: 295 TELGRAGIRWEVEPR 309
>7293566 [R] KOG2497 Predicted methyltransferase
Length = 319
Score = 75.5 bits (184), Expect = 9e-14
Identities = 56/180 (31%), Positives = 88/180 (48%), Gaps = 15/180 (8%)
Query: 99 VVIEETPSLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCGTGIISILYK 158
+ + E+ S + A+GTTG TWEAAL L Y+ QH L ++ELG G G++ I
Sbjct: 112 ITLRESTSFV-AEGTTGLCTWEAALALGDYLLQHRDL--VRGKNIVELGAGAGLLGI--- 165
Query: 159 MIKDSQGDCKAGTIIVTDGDSNLLQQVSTNFQLNGSLSNDGDVNIGFQRLRW---NEDEL 215
M+K + G +++TDG +Q + N LN + ++L W +E
Sbjct: 166 MLKLPALQLQVGQVLLTDGSEPCVQLMRENISLNFP-DTPKEQMPQAEQLNWAAVSEFPW 224
Query: 216 SNYNEIDLILAADVTYDTSVIPDLVKCLSQFKGAHG-----YISCTERNLDTLDAFENEL 270
++ + DL++AADV YD S L+ + G ++ T RN+DTL F +L
Sbjct: 225 DSHAKTDLLIAADVIYDDSQFDALLGAMDYLYSRRGGGLETLLASTVRNVDTLHKFMTQL 284
>CE00292 [R] KOG2497 Predicted methyltransferase
Length = 409
Score = 67.4 bits (163), Expect = 2e-11
Identities = 57/242 (23%), Positives = 107/242 (43%), Gaps = 21/242 (8%)
Query: 38 IVVHRNQY-YAKQLLKQMIQHFESRGSDFVETMYEPYINLLPVGAPDPQDSDIIQYRFDR 96
+ H +Y + +LK +I ++ ++M++ +L+ + + + + D+
Sbjct: 126 VTSHEIRYKFQINVLKNLISELQANNVVVPDSMFDRLGSLMGMKEGEYVERVYLTDGGDK 185
Query: 97 YKVVIEETPSLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCGTGIISIL 156
E + + + GTTG W+A+ L + P + + +LELG G G+ I
Sbjct: 186 VLAKFSEAVNQL-SMGTTGLSVWQASCDLANLFRLIPSEY----NRILELGSGCGVSGIA 240
Query: 157 YKMIKDSQGDCKAGTIIVTDGDSNLLQQVSTNFQLNGSLSNDGDVNIG-----FQRLRWN 211
+ D + +TD D N+L + N N S+ D + + L W
Sbjct: 241 IAKMNDC-------CVTLTDYDDNVLNLLEENIAKNDLKSDTEDSSANNNQATVRSLNWC 293
Query: 212 EDELSNYNE-IDLILAADVTYDTSVIPDLVKCLSQF--KGAHGYISCTERNLDTLDAFEN 268
+ + S + E DLI+AADV YDT+++ L L+ ++CT RN +++ FE+
Sbjct: 294 DFDFSEWKEPTDLIIAADVVYDTALLASLCNVLNLLLRHSKAAIVACTRRNESSIECFEH 353
Query: 269 EL 270
L
Sbjct: 354 HL 355
>Hs22046321 [R] KOG2497 Predicted methyltransferase
Length = 230
Score = 54.3 bits (129), Expect = 2e-07
Identities = 49/171 (28%), Positives = 83/171 (47%), Gaps = 18/171 (10%)
Query: 46 YAKQLLKQMIQHFESRGSDFVETMYEPYIN-LLPVGAPDPQDSDIIQYRFDRYKVVIEET 104
YA L ++I+ E+ ++ ++ +YE L+ +P S ++ V + E+
Sbjct: 69 YAWCFLSELIKKHEAVHTEPLDELYEALAETLMAKESPQGHRSYLLP---SGGSVTLSES 125
Query: 105 PSLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCGTGIISILYKMIKDSQ 164
++I + TTG TW+AAL++ + ++P F TH S +LELG G + + I
Sbjct: 126 MAII-SHSTTGLVTWDAALYIAEWAIENPAAF-THRS-VLELGSGASLTGLAICKI---- 178
Query: 165 GDCKAGTIIVTDGDSNLLQQVSTNFQLNG-SLSNDGDVNIGFQRL----RW 210
C+ I +D S +L+Q+ N LNG SL D N+ R+ RW
Sbjct: 179 --CRPRAYIFSDCHSRVLEQLQGNVLLNGLSLEADITANLDSPRVTMAQRW 227
>YBR271w [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 419
Score = 51.6 bits (122), Expect = 1e-06
Identities = 44/153 (28%), Positives = 68/153 (43%), Gaps = 22/153 (14%)
Query: 115 GFRTWEAALFLCHYMTQHPGLFVTHDSLML-----------ELGCGTGIISILYKMIKDS 163
G++TW ++L L + H T + ML ELG GTG++ + + +
Sbjct: 218 GWKTWGSSLILSQLVVDHLDYLHTTNVNMLANSDIKQIKVLELGAGTGLVGLSWALKWKE 277
Query: 164 QGDCKAGTIIVTDGDSNLLQQVSTNFQLNGSLSNDGDVNIGFQRLRWNE-----DELSNY 218
+ I VTD L ++ TN + N SL+N GD + + L W D+ +
Sbjct: 278 LYGTENIEIFVTD-----LPEIVTNLKKNVSLNNLGDF-VQAEILDWTNPHDFIDKFGHE 331
Query: 219 NEIDLILAADVTYDTSVIPDLVKCLSQFKGAHG 251
NE D+IL AD Y +V +S+F A G
Sbjct: 332 NEFDVILIADPIYSPQHPEWVVNMISKFLAASG 364
>Hs22044526 [R] KOG2497 Predicted methyltransferase
Length = 220
Score = 51.6 bits (122), Expect = 1e-06
Identities = 42/157 (26%), Positives = 74/157 (46%), Gaps = 14/157 (8%)
Query: 46 YAKQLLKQMIQHFESRGSDFVETMYEPYINLLPVGAPDPQDSDIIQYRFDRYKVVIEETP 105
YA+ L ++I+ E+ ++ ++ +YE L + + + E
Sbjct: 69 YARCFLSELIKKHEAVHTEPLDELYEVLAETLMAKESTQGHRSYLLPSGGSF--TLSEIT 126
Query: 106 SLICAQGTTGFRTWEAALFLCHYMTQHPGLFVTHDSLMLELGCGTGIISI-LYKMIKDSQ 164
++I + GTTG TW+A L+L + ++P F +LELG G G+ + + KM
Sbjct: 127 AII-SHGTTGLVTWDATLYLAEWAIENPAAFTNRG--VLELGSGAGLTGLAICKM----- 178
Query: 165 GDCKAGTIIVTDGDSNLLQQVSTNFQLNGSLSNDGDV 201
C+ I +D S +L+Q+ N LNG LS + D+
Sbjct: 179 --CRPQAYIFSDCHSRVLEQLRGNVLLNG-LSLEADI 212
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,933,674
Number of Sequences: 60738
Number of extensions: 832163
Number of successful extensions: 2092
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2071
Number of HSP's gapped (non-prelim): 9
length of query: 308
length of database: 30,389,216
effective HSP length: 106
effective length of query: 202
effective length of database: 23,950,988
effective search space: 4838099576
effective search space used: 4838099576
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)