ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1206 good R KOG1012 General function prediction only Ca2+-dependent lipid-binding protein CLB1/vesicle protein vp115/Granuphilin A, contains C2 domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1206 429876 425407 -1490
(1490 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML072c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 1357 0.0
SPAPYUK71.03c [R] KOG1012 Ca2+-dependent lipid-binding protein C... 434 e-121
YOR086c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 391 e-108
YNL087w [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 362 2e-99
SPCC962.01 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 310 1e-83
ECU01g1250 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 118 6e-26
At1g20080 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 116 2e-25
At3g61050 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 105 7e-22
At5g04220 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 94 1e-18
At1g05500 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 92 5e-18
At5g11100 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 86 3e-16
7301181 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 75 1e-12
At1g53590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 74 2e-12
CE18256 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 69 4e-11
At3g14590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 68 1e-10
SPCP31B10.06 [R] KOG1012 Ca2+-dependent lipid-binding protein CL... 65 8e-10
Hs14149680 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 64 2e-09
Hs5901958 [T] KOG2059 Ras GTPase-activating protein 63 4e-09
Hs21362014 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bin... 62 7e-09
Hs14150054 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 60 3e-08
At2g20990 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 60 3e-08
Hs22045984 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 59 4e-08
At4g21160_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bi... 59 6e-08
At1g50260 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 59 8e-08
CE26302 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 58 1e-07
Hs21361472 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4 56 4e-07
Hs20552506 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 56 5e-07
Hs18584396 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bin... 56 5e-07
At4g05330_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bi... 56 5e-07
Hs5032139 [TU] KOG1028 Ca2+-dependent phospholipid-binding prote... 55 8e-07
Hs4758856 [M] KOG1326 Membrane-associated protein FER-1 and rela... 55 8e-07
7302558 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bindin... 55 8e-07
7295906 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 53 4e-06
Hs4506067 [T] KOG0696 Serine/threonine protein kinase 52 5e-06
CE27898 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bindin... 52 5e-06
CE09100 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bindin... 52 5e-06
SPAC11G7.02 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4 52 9e-06
>YML072c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1545
Score = 1357 bits (3513), Expect = 0.0
Identities = 686/1516 (45%), Positives = 972/1516 (63%), Gaps = 32/1516 (2%)
Query: 1 MLQEEPRTGRPIPVGAETPGDPVTADNNPLLQARTXXXXXXXXXXXXTRDEDEQEKLPDV 60
++ ++ R P G + D N + + + E +++ D+
Sbjct: 33 VINQQERGSDHAPSGHHQYHQLINHDANDTKTSNSVSDVSKGQKTADSNPEGKKQSSKDI 92
Query: 61 IPVSAAQNVVKMSPPNKEGAIDNIPLEVLDPKRFKRAPPRESNMVDTSIDTATYRADQQK 120
S+AQ ++ PN +G+I +PLE L PK F+ AP R+ N DTSI D K
Sbjct: 93 FVASSAQKTNQLPGPNPQGSIGAVPLEGLRPKEFRSAPSRKPNKFDTSITKPGVLDDLGK 152
Query: 121 IESLTKSIEE----EKPEYLFPWFSVGGFNVVKNPATQMKSSRVVKSYVVENYYNDWYSN 176
++ K I+E + + LFPW +VG F+ + K SRV+K+Y++EN+YNDWY N
Sbjct: 153 LDE--KDIKEKFHLDSDDKLFPWQNVGEFHASGKGSPNTKMSRVIKAYILENFYNDWYCN 210
Query: 177 TAGVVGTCFFSWLLSYLNFSWWSLGFVFFCTASVYRAEFRRFNRNIRDDMKRITVEETLS 236
A V+GTCFFSWL +Y+ FSWWS+ F+F TA+VY AE+ RFNRNIRDD+KR+TVEETLS
Sbjct: 211 IATVLGTCFFSWLFAYIGFSWWSMIFIFLGTATVYNAEYTRFNRNIRDDLKRVTVEETLS 270
Query: 237 DRTESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAP 296
DR ES+ WLNSFLSKFW IYMPVLSQQVKD VNPQLAGVAPGYGIDAL+++EFTLG+KAP
Sbjct: 271 DRVESTTWLNSFLSKFWVIYMPVLSQQVKDNVNPQLAGVAPGYGIDALAIDEFTLGSKAP 330
Query: 297 TIDAIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSL 356
TI IKSYTK G VEMDW+ +FTP+D S+MT EA+ KINPKI+LGVT+GK FVSK++
Sbjct: 331 TIKGIKSYTKTGKNTVEMDWSFAFTPSDVSDMTATEAREKINPKISLGVTLGKSFVSKTM 390
Query: 357 PVLMEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLP 416
P+L+EDINVAG I ++FG FPNIK VS+ +LEPPLIDFALKPIGGDTLGLD+MSFLP
Sbjct: 391 PILVEDINVAGKMRIKVEFGKAFPNIKIVSLQLLEPPLIDFALKPIGGDTLGLDVMSFLP 450
Query: 417 GLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQDAIGVVAVTIDSASDLKTSDILS 476
GLK+FVK +INSN+GPML+ PN LDI+VE+IMAAQS++AIGV+AVTI SA LK SD ++
Sbjct: 451 GLKSFVKNIINSNIGPMLFPPNHLDINVEDIMAAQSKEAIGVLAVTIASADSLKGSDFIT 510
Query: 477 TSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLDQKLNLTCYDFNDV 536
+VDPYI TTE + G + ++RT++KS+ +NPRWNETKYLL+N+L+QKLNL C+DFNDV
Sbjct: 511 NTVDPYIVMTTEDAVPGTDEEVRTSIKSNVKNPRWNETKYLLLNTLEQKLNLKCFDFNDV 570
Query: 537 RKDALIGSFDIDLSELYQKPAQEHLSKDLVARGKSKGVLNYSIDWFPVMXXXXXXXXXXX 596
RKD +IG +DL++L Q P ++ + +L + KSKG+L+YS+ WFPV
Sbjct: 571 RKDTVIGDLQLDLADLLQNPVLDNQTAELRSGTKSKGILHYSLHWFPVKEDKSEEKAVER 630
Query: 597 XXXXX-----XXXXXXXXXXXXXXXXXXXXXVGIMKFTLHKVKYLNKSAALTGFLSPCAE 651
VGI K TL KVKYL+ ++++TG LSPCAE
Sbjct: 631 AEAKAKGKKEDENEDTTEKEEDENEESSQTDVGIAKITLQKVKYLDTTSSMTGSLSPCAE 690
Query: 652 LFIDGKKVKSYRTLRRINEPSWEETIEILIPXXXXXXXXXXXYDDQMHGKRQLAEYTASM 711
LFIDG+KVKSYRTLRRINEPSW ETIE+L+P +DD+M+GK + EY++S+
Sbjct: 691 LFIDGQKVKSYRTLRRINEPSWNETIEVLVPSKSNSKFVLKIFDDRMNGKALICEYSSSL 750
Query: 712 EDVIELFEQGQTFVQASPQGEIYITSSWKPVAMAGAFKVHNTVREPLAALRVHLKDATIS 771
+D++ + Q FV+ SPQG+IY+ SWK + M GAF N+V EP+ +++ +KDA I
Sbjct: 751 DDIMTTLDTAQEFVKGSPQGDIYLDVSWKSIEMTGAFAAANSVSEPIGCIKLDVKDAIIK 810
Query: 772 SDDFSGVGDIDPYVKVMVNKHLIYRTSYFSETKNPVFNNVIYIPITSENQNVSLHMYDYQ 831
D SGVGD+DPY V +N+ ++Y++ Y S+T +P+F+N Y+PI S NQ ++L +DYQ
Sbjct: 811 G-DLSGVGDVDPYYTVSLNRRVLYKSIYHSDTDHPIFDNSTYVPIFSPNQILTLEFHDYQ 869
Query: 832 KVGKDRLVGIYQISSSKLIRKDSDTGRYIPKFTDEKSLKCILVNNKGKTMKSFMNLSCSV 891
K+GKDR +G QI +S + +KD +G+Y+ E+ K L +++ K +S +N+S +
Sbjct: 870 KIGKDRFIGSVQIPTSNVFKKDPKSGKYVGNNGKEEISKLKLKDHEHKVTESIVNVSTTF 929
Query: 892 VPAIPVYYPHELPAVDQLAXXXXXXXXXXXXXXXXXXXXMDKNPKEYEVVEAEDKFEKDI 951
+P VY P EL V++L M+KNPKE+EV E ED F+ D
Sbjct: 930 IPINLVYSPEELVNVEKLEKELKEKKKKFEATQEENEQEMEKNPKEWEVAEIEDPFDSDE 989
Query: 952 KKLNRKERMNIDQLMEHNSGICSFQLVESSLSPSSVYLQLLYDDIAFPVSISQKSNAGKI 1011
KK+NRK ++++++L++ SGI S Q++E +LSPSS YL++L DDI++PV I K + GK+
Sbjct: 990 KKINRKAKLSLNELIKQKSGILSMQILEGTLSPSSAYLEILADDISYPVFICMKPSQGKL 1049
Query: 1012 PPESGNFFVRDLKHSTMTFRITKKPIVKNVDDXXXXXXXXXXXXLKEAIEDPTTISFKNG 1071
E N F+RDL +S + FR++KK I K+ DD LK+A E+P ++F NG
Sbjct: 1050 NSEMANIFIRDLNYSKLHFRVSKKHIAKDSDDVISETSYSTLKLLKQAYEEPMWLNF-NG 1108
Query: 1072 AQVKIRLLFNPTAIELPLSETVLDTGILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKI 1131
+++K+R L+ PT+++LP SE+V DTG L+ IS + S DRNG SDP + + ++ +K+
Sbjct: 1109 SKMKVRFLYTPTSVKLPSSESVEDTGYLNIKLISGHGLKSADRNGYSDPFVHIFVNDKKV 1168
Query: 1132 FQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKK 1191
F+S + KKTL+PVWNE K+P+ SRS+N++ V DWDRAG ND L + DV + K
Sbjct: 1169 FKSNIKKKTLDPVWNEDAKIPILSRSKNQVIFNVLDWDRAGDNDDLGQASLDVSSLEVGK 1228
Query: 1192 EESFTLKLSPQGTVKFKSTFIPNYNRPPVDVSEGGLAGASLK-------AVGNVANFGVD 1244
++ L L+ QG++K + +F P Y +P D+ +GG+ +K A +A G+
Sbjct: 1229 TYNWNLNLNTQGSIKLQGSFNPEYIKPSFDIVKGGITDKPMKIASGAAHATVGIAGTGIG 1288
Query: 1245 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSKNGGEXXXXXXXXXXXXXXXX 1304
+K E
Sbjct: 1289 AATGVATGGLKKGGHLLKSLGGNPMKRSKSSNGNESNGAKKSSEKKSFDRRSPSNLNSTS 1348
Query: 1305 XXXXXX-----XVPNTSYASVRAQT---APTVSAKGDSSIQDPHGAIHKRSLSAASNFSR 1356
VPNTSYA V++ + PT + S+ +D + + R S AS+F+R
Sbjct: 1349 VTPRASLDYDPSVPNTSYAPVQSASPVVKPTDNTSSSSNKKDTPSS-NSRGHSRASSFAR 1407
Query: 1357 STIP-GSHKGKATIIGAENLGKAVQVRVSLAQGGRIRHLHRTHERKADENGVCQFNEVVD 1415
+ P G++ G T++ AEN+ K VQ+++SL QGGR++H+++T +KA+ +GV F+E
Sbjct: 1408 TLAPHGTYNGFITVVAAENVAKHVQIKISLTQGGRLKHIYKTKSQKANNDGVAVFDEECS 1467
Query: 1416 FKATGDSSIVLGAVAHHKFSKDTELGVSQINLSDPQLQQEGQVALRLGSGHVIFKIEYPL 1475
FKA+ ++++VLGA++H + S+D +LG++QINL DPQ+QQ+GQ++++LG GH+I KI Y
Sbjct: 1468 FKASPEANLVLGAISHQRLSRDKDLGIAQINLGDPQIQQDGQISVKLGDGHLIVKINYGK 1527
Query: 1476 D--GEIPPTPEIPNKY 1489
D G++PP PE+P +Y
Sbjct: 1528 DKNGQVPPVPEVPQEY 1543
>SPAPYUK71.03c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1225
Score = 434 bits (1115), Expect = e-121
Identities = 292/1088 (26%), Positives = 503/1088 (45%), Gaps = 73/1088 (6%)
Query: 180 VVGTCFFSWLLSYLNFSWWSLGFVFFCTASVYRAEFRRFNRNIRDDMKRITVEETLSDRT 239
V+ T S+L YL F + SL + YR RR N +DD R L + +
Sbjct: 159 VLYTAVMSFLFGYLRFGFLSLFIIMAVCIQYYRICDRRVKVNFKDDYTRYLSTRKLENDS 218
Query: 240 ESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTID 299
E+ WLN+FL +FW I+ P LS+++ + + L+ P + ID+++L+EFTLGTK+P +
Sbjct: 219 ETVTWLNTFLQQFWYIFEPSLSERITEITDQILSENVPSF-IDSMALSEFTLGTKSPRMG 277
Query: 300 AIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLPVL 359
I+SY K + V MD ++F+PND S++T +E I PKIAL + IGK S +PVL
Sbjct: 278 FIRSYPKTEEDTVMMDLRLAFSPNDISDLTGREIAACIKPKIALDLKIGKSIASAKMPVL 337
Query: 360 MEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPGLK 419
+ED++ G + +K D +P KTV ++ E P+ + LKP+GGD G DI + +PGL
Sbjct: 338 IEDLSFTGNLRVKVKLIDKYPYAKTVGLTFTEKPVFSYILKPLGGDKFGFDIGN-IPGLT 396
Query: 420 TFVKTMINSNVGPMLYAPNQLDIDVEEIM-AAQSQDAIGVVAVTIDSASDLKTSDILSTS 478
TF+ I++ +GPM+Y+PN ++D+E +M AA A+G V + K D L +
Sbjct: 397 TFITEQIHNTLGPMMYSPNVYELDIESMMGAAGLNTALGAVEFKLRKGDGFK--DGLGGA 454
Query: 479 VDPYIKFTTEKGIIGNENDL---RTTVKSDTRNPRWNETKYLLVNSLDQKLNLTCYDFND 535
VDPY+ +I N D ++ V +T +P +NET Y ++NS + LNL YDFND
Sbjct: 455 VDPYV-------VIKNSADRVIGKSKVAHNTGSPVFNETFYSVLNSFSENLNLEVYDFND 507
Query: 536 VRKDALIGSFDIDLSELYQKPAQEHLSKDLVARGKSKGVLNYSIDWFPVMXXXXXXXXXX 595
+R D L+GS + L+ L P +L +GK+ G LNY + + V+
Sbjct: 508 IRSDKLLGSAVLPLATLEAMPVTNDAFVELTLKGKTVGRLNYDMKFHAVV---------- 557
Query: 596 XXXXXXXXXXXXXXXXXXXXXXXXXXXVGIMKFTLHKVKYLNKSAALTGFLSPCAELFID 655
G+++FT+H+ K L+ + + A+L I+
Sbjct: 558 ---------------PDSGEEITKVDGPGVLQFTVHQCKELSNDPSKRP--TAYAKLIIN 600
Query: 656 GKKVKSYRTLRRINEPSWEETIEILIPXXXXXXXXXXXYDDQMHGKRQLAEYTASMEDVI 715
K+V + R +++ N PSWEE+ L+P + ++ + S++D+
Sbjct: 601 NKEVYTTRKIKKNNNPSWEESFGTLLPEGKNATLGVQIFTEE--SEHPFGTANVSLQDLF 658
Query: 716 ELFEQGQTF--VQASPQGEIYITSSWKPVAMAGAFKVHNTVREPLAALRVHLKDATISSD 773
+ G + +Q +P G + ++ WKP + + P+ A+R+HL+ A
Sbjct: 659 AATKTGLLWFPLQHAPSGRVRMSVMWKPAQLNNDSISSMALATPIGAIRIHLRSANNLHS 718
Query: 774 DFSGVGDIDPYVKVMVNKHLIYRTSYFSETKNPVFNNVIYIPITSENQNVSLHMYDYQKV 833
G D Y ++M + +RT + NP ++ +Y P+ +++ L + +Y
Sbjct: 719 KIPG-KKCDSYARIMSHNTKQFRTVVIASNVNPFWDEYMYAPVITKHDIFFLQVMNYNSS 777
Query: 834 GKDRLVGIYQISSSKLIRKDSDTGRYIPKFTDEKSLKCILVNNKGKTMKSFMNLSCSVVP 893
G+D+L+G I+ S I + + + ++ D + L L + +G + + C P
Sbjct: 778 GEDKLIGQTPINISNFINQGENGA--LMEYHDPRELTVPLSSTRGIKGNATITFKCDFFP 835
Query: 894 AIPV--YYPHELPAVDQLAXXXXXXXXXXXX-XXXXXXXXMDKNPKEYEVVEAEDKFEKD 950
+ P PA + +D + K
Sbjct: 836 SAVTTSLSPDVTPAPKASSTVATDKVNIEVLPESQKTPTAVDNTSTSRGSTSVKTSKPKK 895
Query: 951 IKKLNRKERMNIDQLMEHNSGICSFQLVESSLSPSSVYLQLLYDDIAFPVSISQKSN--A 1008
I +L ++ ++ SG F ++ ++ + L + DD+ + +S N
Sbjct: 896 ISELLMPSEA-VNAALDFESGFMGFDIISYKIAKPAQELAIFLDDLPHHIFLSSALNVTG 954
Query: 1009 GKIPPESGNFFVRDLKHSTMTFRITKKPIVKNVDDXXXXXXXXXXXXLKEAIEDPTTISF 1068
G E GN F+R L++S TF++ + D + + P I+F
Sbjct: 955 GATLHEYGNTFIRQLEYSQCTFKLL------DGDKEVGSKTMLSRDLISKGATKPLEIAF 1008
Query: 1069 KNGAQVKIRLLFNPTAIELPLSETVLDTGILDTTFISADDVPSHDRNGKSDPMIIVRIDG 1128
+GA + + P ++L E + G + I A D+P+ D NGKSDP ++ + G
Sbjct: 1009 PDGASILVAFRLTPVPVKLEEVEMYENMGEMTVDVIKATDLPAADSNGKSDPFVVFELQG 1068
Query: 1129 EKIFQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMT 1188
E+++++ K+TLNP +NE ++ +P + V+DWD +D L D + +
Sbjct: 1069 EEVYRTKTHKRTLNPTFNESFEVELPCKQTCNFVANVFDWDFGNKDDHLGSCVIDCKLLQ 1128
Query: 1189 PKKEESFTLKL-SPQGTVKFKSTFIPNY--------NRPPVDVSEG---GLAGASLKAVG 1236
+++ ++ + L S QG + + T P + N V+ G + G LK +
Sbjct: 1129 QQQQTNYEIPLDSKQGVLYLRITLSPKWVLRSKRAGNSSLVEGILGQTASIVGMPLKGIS 1188
Query: 1237 NVANFGVD 1244
V N VD
Sbjct: 1189 TVGNVAVD 1196
>YOR086c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1186
Score = 391 bits (1004), Expect = e-108
Identities = 289/1107 (26%), Positives = 514/1107 (46%), Gaps = 75/1107 (6%)
Query: 95 KRAPPRESNMVDTSIDTATYRADQQKIESLTKSIEEEKPEYLFPWFSVGGFN-----VVK 149
K PP+ ++S ++ +A ES +SI E W +GG+ +
Sbjct: 27 KLEPPKTKEETESSKSVSSEKAAHASDESFKRSIHEAS---YVGWKQIGGWEDKDELTLD 83
Query: 150 NPATQMKSSRVVKSYVVENYYNDWYSNTAGVVGTCFFSWLLSYLNFSWWSLGFVFFCTAS 209
+ M + + + ++ Y DWY + A S+ L + FS S FV T+
Sbjct: 84 DELMDMTRETFLDNIIPDSLYGDWYHSVAIFFIGGVASFALGHYKFSMGSAFFVIVITSL 143
Query: 210 VYRAEFRRFNRNIRDDMKRITVEETLSDRTESSVWLNSFLSKFWTIYMPVLSQQVKDAVN 269
+YR +++ +IR+ +++ + + + ES WLN+FL K+W I P +SQ + N
Sbjct: 144 LYRTSAKKYRGSIRELVQKEFTVQKVENDYESLEWLNAFLDKYWPILEPSVSQLIVQQAN 203
Query: 270 PQLAG--VAPGYGIDALSLNEFTLGTKAPTIDAIKSYTKKGDGVVEMDWTVSFTPNDESN 327
Q+A P + I L ++E TLG K P +D +K++ VV MDW +SFTP+D +
Sbjct: 204 EQMATNEAIPKF-ITQLWIDELTLGVKPPRVDLVKTFQNTASDVVVMDWGISFTPHDLCD 262
Query: 328 MTPKEAKNKINPKIALGVTIGKGFVSKSLPVLMEDINVAGTAHITLKFGDVFPNIKTVSV 387
M+ K+ +N +N L V K F ++PV + DI A + K FP+++TV++
Sbjct: 263 MSAKQVRNYVNE---LAVVKAKIF-GITIPVSVSDIAFKAHARVKFKLMTPFPHVETVNI 318
Query: 388 SMLEPPLIDFALKPIGGDTLGLDIMSFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEI 447
+L+ P DF G +I++ +PGL T ++ M +GP+L P L +++ ++
Sbjct: 319 QLLKVPDFDFVATLFGRSIFNWEILA-IPGLMTLIQKMAKKYMGPILLPPFSLQLNIPQL 377
Query: 448 MAAQSQDAIGVVAVTIDSASDLK-TSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDT 506
++ S +IG++ +T+ +A LK TS IL+ S+DPY+ F I +R DT
Sbjct: 378 LSG-SNLSIGILEITVKNAKGLKRTSSILNESIDPYLSFEFNDISIAKTRTVR-----DT 431
Query: 507 RNPRWNETKYLLVNSLDQKLNLTCYDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKDLV 566
NP W+ET Y+L+NS L ++ YD KD ++G +L+ L+ K Q +L +
Sbjct: 432 LNPVWDETLYVLLNSFTDPLTISVYDKRAKLKDKVLGRIQYNLNTLHDKTTQRNLKAQFL 491
Query: 567 ARGKSKGVLNYSIDWFPVMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGIM 626
K G L + + +FP + GI
Sbjct: 492 RNSKPVGELTFDLRFFPTL--------------------EEKKLPDGSVEELPDLNTGIA 531
Query: 627 KFTLHK-VKYLNKSAALTGFLSPCAELFIDGKKVKSYRTLRRINEPSWEETIEILIPXXX 685
K + + ++ + +T ++ E++++ K V + W E +I
Sbjct: 532 KVVVEEGSRFAEEEQKVTAYV----EVYLNAKLVLTTGKATDTGTLKWNSDYEAVIADRR 587
Query: 686 XXXXXXXXYDDQMHGKRQLAEYTASMEDVIELFEQGQTFVQASPQ-GEIYITSSWKPVAM 744
D + ++ ++ D+I+ + + + Q G+I IT+ W+PV +
Sbjct: 588 KTRYKFVVKDGK---GEEIGSTIQTLNDLIDRSQVNKNLIPLKNQKGDIKITTYWRPVRL 644
Query: 745 -AGAFKVHNTVREPLAALRVHLKDATISSDDFSGVGDIDPYVKVMVNKHLIYRTSYFSET 803
G+ V T P+ A+RV ++ A + G IDPY KV+VN RT + S+T
Sbjct: 645 EIGSNSVAYT--PPIGAIRVFIEKAN-DLRNLEKFGTIDPYCKVLVNGLSKGRTDFKSQT 701
Query: 804 KNPVFNNVIYIPITSENQNVSLHMYDYQKVGKDRLVGIYQISSSKLIRKDSDTGRYIPKF 863
NPV+N VIY+ +TS NQ ++L D + V KDR +G + ++ L +KD + +Y +
Sbjct: 702 LNPVWNQVIYVAVTSPNQRITLQCMDVETVNKDRSLGEFNVNVQDLFKKD-ENDKY-EET 759
Query: 864 TDEKSLKCILVNNKGKTMKSFMNLSCSVVPAIPVYYPHELPAVDQLAXXXXXXXXXXXXX 923
DEK+ LV K K K + S PA+PV E+ +D++
Sbjct: 760 IDEKAKVGRLVMPK-KKPKGTITYYTSFYPALPVLTLEEIQDLDKVNKKKKALELRKSAI 818
Query: 924 XXXXXXXMDK---NPKEYEVVEAEDKFEKDIKKLNRKERMNIDQLMEHNSGICSFQLVES 980
DK + + EV E ED + + ++++++ +L+++N G+ + ++
Sbjct: 819 DEKKISKEDKAKFDQEWNEVKELEDMY-------SNRQKLDLPELLQYNQGVLAVTVLNG 871
Query: 981 SLSPSSVYLQLLYDDIAFPVSISQKSNAGKIPPE-SGNFFVRDLKHSTMTFRITKKPIVK 1039
L S +Y+Q +DD P +S + + + SG+ +++L S TFR+ K
Sbjct: 872 ELPDSGLYVQAFFDDNGHPRFVSPRIPSRIVKNGWSGDVIIKELDKSITTFRVAKNKNYN 931
Query: 1040 NVDDXXXXXXXXXXXXLKEAIEDPTTISFKNGAQVKIRLLFNPTAI---ELPLSETVLDT 1096
V+ +K P+ + K+ L + I +LP ++ + ++
Sbjct: 932 RVEKCVCEVELPTQELVKNCYYKPSILHLSGEGSAKLMLQISWFPIDTKQLPANDLITNS 991
Query: 1097 GILDTTFISADDVPSHDRNGKSDPMIIVRIDGEK--IFQSAVVKKTLNPVWNEKVKLPVP 1154
G L SA+++ + D NG SDP + I+ E+ +++ VVKKTLNP WN++ + +
Sbjct: 992 GDLTIMSRSAENLIASDLNGYSDPYLKYYINNEEDCAYKTKVVKKTLNPKWNDEGTIQIN 1051
Query: 1155 SRSRNKIAVQVYDWDRAGSNDLLAETE 1181
+R + + ++V DWD ++D + E
Sbjct: 1052 NRLNDVLRIKVMDWDSTSADDTIGTAE 1078
>YNL087w [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1178
Score = 362 bits (929), Expect = 2e-99
Identities = 288/1144 (25%), Positives = 520/1144 (45%), Gaps = 77/1144 (6%)
Query: 118 QQKIESLTKSIEEEK--PEYLFPWFSVGGFN-----VVKNPATQMKSSRVVKSYVVENYY 170
+ ++E+ SI EK P Y+ W VGG+ ++ + + + + + +Y
Sbjct: 38 KSEVETNGTSIVREKQDPSYV-GWKQVGGWEEKDELTSEDLLVDVNKDTFLGNLLPDKFY 96
Query: 171 NDWYSNTAGVVGTCFFSWLLSYLNFSWWSLGFVFFCTASVYRAEFRRFNRNIRD-DMKRI 229
DWY A ++ S++L Y FS S+ V T +YR +++ ++RD K
Sbjct: 97 GDWYHEVAILIIAGLCSFVLGYFKFSLASVLIVMLTTGMLYRTSSKKYRESLRDLAQKEQ 156
Query: 230 TVEETLSDRTESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLA-GVAPGYGIDALSLNE 288
TVE+ SD ES WLN+FL K+W I P +SQQ+ D N L+ VA I A+ L++
Sbjct: 157 TVEKITSDY-ESVEWLNTFLDKYWPIIEPSVSQQIVDGTNTALSENVAIPKFIKAIWLDQ 215
Query: 289 FTLGTKAPTIDAIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIG 348
FTLG K P IDAIK++ VV MD +SFTP+D S++ K+ +N +N + L I
Sbjct: 216 FTLGVKPPRIDAIKTFQNTKSDVVVMDVCLSFTPHDMSDLDAKQCRNYVNSNVVLKAKI- 274
Query: 349 KGFVSKSLPVLMEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLG 408
+PV + DI + P ++T+++ +LE P +DF + +G
Sbjct: 275 ---FGMDIPVSVADIFFQVFVRFRFQLMTTLPLVETINIQLLEVPEVDFISRLLGNSVFN 331
Query: 409 LDIMSFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQDAIGVVAVTIDSASD 468
+I++ +PGL ++ M + P+L P L +++ +++ +++ IGV+ + + +A
Sbjct: 332 WEILA-IPGLMRLIQKMAFKYLSPVLLPPFSLQLNIPQLL-SKTGLPIGVLEIKVKNAHG 389
Query: 469 L-KTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLDQKLN 527
L K ++ +VDPY+ F I+G +T V ++ NP WNE+ Y+L+ S L
Sbjct: 390 LRKLVGMIKKTVDPYLTFELSGKIVG-----KTKVFKNSANPVWNESIYILLQSFTDPLT 444
Query: 528 LTCYDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKDLVARGKSKGVLNYSIDWFPVMXX 587
+ YD + D +G+ +L++L+ ++ + K G L + + +FP +
Sbjct: 445 IAVYDKRETLSDKKMGTVIFNLNKLHANHYHKNEKVHFLRNSKPVGELTFDLRFFPTI-- 502
Query: 588 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGIMKFTLHKVKYLNKSAALTGFLS 647
GI K T+ ++K L++ L+
Sbjct: 503 ------------------EPKKLLNGDEEPLPDMNTGITKITIRELKGLDE---LSDKKF 541
Query: 648 PCAELFIDGKKVKSYRTLRRINEPSWEETIEILIPXXXXXXXXXXXYDDQMHGKRQLAEY 707
AEL+++ + V + + +R W ++ D + ++
Sbjct: 542 VFAELYVNAELVMTTKKEKRTAHLKWNSDYYSVVTDRRKTICRFVLKD---QSGKVISSS 598
Query: 708 TASMEDVIELFEQGQTFVQ-ASPQGEIYITSSWKPVAM-AGAFKVHNTVREPLAALRVHL 765
+ +I+ E + ++ + +GE+ +T+ W+PV + G V T P+ LRV +
Sbjct: 599 VQPLNHLIDRTEVNKEWIPLRNGKGELKVTTYWRPVDIDLGLKSVGYTT--PIGMLRVFI 656
Query: 766 KDA-TISSDDFSGVGDIDPYVKVMVNKHLIYRTSYFSETKNPVFNNVIYIPITSENQNVS 824
A + + D +G I PY KV VN RT+ ET NP++N IY+ +TS Q VS
Sbjct: 657 NKAENLRNPD--SLGKISPYAKVSVNGVARGRTNERIETLNPIWNQSIYVSVTSPLQKVS 714
Query: 825 LHMYDYQKVGKDRLVGIYQISSSKLIRKDSDTGRYIPKFTDEKSLKCILVNNKGKTMKSF 884
+ + G D +G I + + KD+D I F D L+ KG +K
Sbjct: 715 IDCFGIDTNGDDHNLGSLNIQTQNIYHKDNDDKYTI--FIDNAPRTGNLIGKKG--VKGT 770
Query: 885 MNLSCSVVPAIPVYYPHELPAVDQLAXXXXXXXXXXXXXXXXXXXXMDKNPKEYEVVEAE 944
+ S P +PV E VD++ +K + E
Sbjct: 771 VTYYLSFYPVVPVLSLEEAKEVDEINEKKDKLEKQKSTLDDKNISKEEKERIKKEEFRLT 830
Query: 945 DKFEKDIKKLNRKERMNIDQLMEHNSGICSFQLVESSLSPSSVYLQLLYDDIAFPVSISQ 1004
+K++ + K ++++D+L+++N+G+ ++ L +Y+Q +D + S
Sbjct: 831 EKYD----MYSYKMKLDLDELLQYNAGVLGVTVLGGELPQPGLYVQTFFDSCGYAAITSA 886
Query: 1005 KSNAGKIPPE-SGNFFVRDLKHSTMTFRITKKPIVKNVDDXXXXXXXXXXXXLKEAIEDP 1063
K+ I SG+F +++L+ S TFR+TK ++ ++ P
Sbjct: 887 KNAIRTIKTGWSGDFMIKELEWSVTTFRVTKTKDANKAENFICEVNIPTIELVRNCYYKP 946
Query: 1064 TTISF--KNGAQVKIRLLFNP-TAIELPLSETVLDTGILDTTFISADDVPSHDRNGKSDP 1120
+ ++ K A++ +++ + P TA ELP S+ + ++G L T SA+++ ++NG SDP
Sbjct: 947 SVLNLIGKKSAKLLVQVSWFPVTATELPQSDLITNSGDLKITAKSAENLIGVNKNGYSDP 1006
Query: 1121 MIIVRIDGEK---IFQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLL 1177
+ ++ + F++AV KKTLNP WNE + V +R + + + V D++ SN +
Sbjct: 1007 YVEFFLNEKSTSPFFKTAVQKKTLNPTWNESKTIEVSNRVNDYLTINVKDYESTNSNRSI 1066
Query: 1178 AETEWDVRDMTPKKEESFTLKL-----SPQGTVKFKSTFIPNYNRPPVDVSEGGLAGASL 1232
+ + + P+ + +F + L G + + F P Y V E GL +
Sbjct: 1067 GKAVVPLSTIDPESDTTFNIPLVGPKGEDGGVLHLEFEFEPRYTTNVVK-REAGLGNFAT 1125
Query: 1233 KAVG 1236
K +G
Sbjct: 1126 KGLG 1129
>SPCC962.01 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1429
Score = 310 bits (794), Expect = 1e-83
Identities = 265/1087 (24%), Positives = 478/1087 (43%), Gaps = 85/1087 (7%)
Query: 152 ATQMKSSRVVKSYVVENYYN-----DWYSNTAGVVGTCFFSWLLSYLNFSWWSLGFVFFC 206
A Q S RV S ++E Y + W+ +T+ ++ SW+ S L F ++ L F+
Sbjct: 176 ALQWDSHRVEPS-ILETYSSYKLTGQWWQSTSILLAVSILSWIASKLWFRFFILFFIIIT 234
Query: 207 TASVYRAEFRRFNRNIRDDMKRITVEETLSDRTESSVWLNSFLSKFWTIYMPVLSQQVKD 266
VY + RNIR+++ + ++ E+ W N+ L +FW + P +S+ V
Sbjct: 235 GTIVYGSCMISVRRNIREEVVQELSKKNGDVDYETMSWFNTLLQRFWMLNEPEISKSVST 294
Query: 267 AVNPQLAGVAPGYGIDALSLNEFTLGTKAPTIDAIKSYTKKGDGVVEMDWTVSFTPNDES 326
+V +A P + I + + FTLG+KAP ID ++++ VV MD S TPND
Sbjct: 295 SVEQSIAEYLPSF-IKEAAFSTFTLGSKAPRIDRVRTHPPVERDVVLMDVDFSLTPNDNY 353
Query: 327 NMTPKEAKNKINPKIALGVTIGKGFVSKSLPVLMEDINVAGTAHITLKFGDVFPNIKTVS 386
++ K ++N I+L + G G S P+ ++D+ ++G I +P I+T S
Sbjct: 354 DVNDSSLKCRVNSLISLVIKFGFGKYMFSFPITIKDLRLSGKLRIRWGLSSDYPFIQTAS 413
Query: 387 VSMLEPPLIDFALKPIGGDTLGLDIMSFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEE 446
S LE P++ ++PI L DI ++PG+ FV + + M+ PN D D+
Sbjct: 414 FSFLETPIVYANIRPIDIPFLDADIF-YIPGIGQFVSEQLGLLLNSMVLWPNMFDYDLSA 472
Query: 447 IMAA-QSQDAIGVVAVTIDSASDLKTSD-ILSTSVDPYIKFTTEKGIIGNENDLRTTVKS 504
+MA S A+GVV + I SA + SD + +I TT G E+ RT ++S
Sbjct: 473 MMAGIASGTAVGVVGLKIYSARRGEVSDSSIDRKPSSFITVTTS----GREHG-RTPIRS 527
Query: 505 DTRNPRWNETKYLLVNSLDQKLNLTCYDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKD 564
+T +P ++ T Y+++NSL+ L L+ YD N + L+G+ ID LY++ + +
Sbjct: 528 NTFSPTFDTTIYVVINSLNDPLKLSLYD-NSGKSPILVGTTYIDPRSLYERGFIGDIYQF 586
Query: 565 LVARGKSKGVLNYSIDWFPVMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVG 624
L + G + + +FP + G
Sbjct: 587 LY-NAVNVGSVAFDATFFPSLLPKKTMDGSKIEPPESSK--------------------G 625
Query: 625 IMKFTLHKVKYLNKSAALTGFLSPCAELFIDGKKVKSYRTLRRINEPSWEETIEILIPXX 684
I+ L V L + LT S L++D K+V S +T++ ++ I
Sbjct: 626 ILNVNLGCVNNLTELTELTKKSSLKYVLYVDSKEVAS-KTIKFVDRTPISLQTNAYIENN 684
Query: 685 XXXXXXXXXYDDQMHGKRQLAEYTASMEDVIELFEQGQTFVQASPQGEIYITSSWKPVAM 744
+D + ++ +A + + +++ FV+ +P+ I I S W PV +
Sbjct: 685 KKSSIKVAVFDVK-SPEKAIATVSVPLPELLHEGYDTFHFVE-NPKATIDIESFWTPVDV 742
Query: 745 AGAFKVHNTVREPLAALRVHLKDATISSDDFSGV----GDIDPYVKVMVNKHLIYRTSYF 800
+ + +R+ + I ++D V DPY +V+V ++ RT Y
Sbjct: 743 VEEKSAKTYIDNLVGVMRL----SVIKANDLVNVELPTRKSDPYARVIVGNSVVARTVYT 798
Query: 801 SETKNPVFNNVIYIPITSENQNVSLHMYDYQKVGKDRLVGIYQISSSKLIRKDSDTGRYI 860
NP++N ++Y+PI ++ + + L DY++ G DR +G I+ K IR R
Sbjct: 799 PNNLNPIWNEILYVPIMADTKTIDLEAMDYEESGNDRSLGYASINVQKYIRNAKRLDRSA 858
Query: 861 ---PKFTDEKSLKCILVNNKGKTMKSFMNLSCSVVPAIPVYYPHELPAVDQLAXXXXXXX 917
F + L + KG++++ ++++C P + + + +
Sbjct: 859 LASTVFGTSEVNALTLTSRKGQSVRGTISVNCDYRPCLRLNTDNSSKQSSENVQSATD-- 916
Query: 918 XXXXXXXXXXXXXMDKNPKEYEVVEAEDKFEKDIKKLNRKERMNIDQLMEHNSGICSFQL 977
A+D + + + +++++ +++ SG +
Sbjct: 917 ---------------------PTTPAKDNSTSNAETSSITSVISVNEALQYPSGFALISI 955
Query: 978 VESSLSPSSVYLQLLYDDIAFPVSISQKSNAGKIPPES--GNFFVRDLKHSTMTFRITKK 1035
V + L V L++ D+ AFP I+ K P S G VR+L+ S TF++T
Sbjct: 956 VSADLQDVGVDLRVFTDNAAFPF-ITTPIAKTKTPRWSSFGISMVRELQFSETTFQLTDG 1014
Query: 1036 PIVKNVDDXXXXXXXXXXXXLKEAIEDPTTISF--KNGA--QVKIRLLFNPTAIELPLSE 1091
K + EA+ P ++ NG V++ + + P + L E
Sbjct: 1015 --AKKDPKVVCEHSVKTLDLVSEALGRPYSVEIPGSNGQLNHVRLSITYMPVPMTLNPME 1072
Query: 1092 TVLDTGILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKL 1151
+ +++G L ++P D SDP ++++++GE F+S V+KK LNPVWNE+ +
Sbjct: 1073 SYINSGSLHFMLQDGQNLPIGDIR-SSDPFVVLKLNGESAFKSKVIKKNLNPVWNEEADI 1131
Query: 1152 PVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL-SPQGTVKFKST 1210
V +R + + + YDWD D+L + D+ + P E ++KL S GT+
Sbjct: 1132 VVQNRVLDVLELVCYDWDMGEKPDVLGTSNIDLLSLEPNVESQQSIKLDSKTGTINASLR 1191
Query: 1211 FIPNYNR 1217
F+P ++R
Sbjct: 1192 FVPGWHR 1198
>ECU01g1250 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 1018
Score = 118 bits (296), Expect = 6e-26
Identities = 93/348 (26%), Positives = 168/348 (47%), Gaps = 29/348 (8%)
Query: 188 WLLSYLNFSWWSLGFVFFCTASVYRAEFRRFNRNIRDDMKRITVEETLSDRTESSVWLNS 247
+++ S +L FV + VY + RF R+++ + +E + E+ WLN
Sbjct: 72 YIIGKTGLSVVNLFFVGYVIFFVYNRKIERFTRSLKSLVYHSARQEKARNNGETVEWLNY 131
Query: 248 FLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTIDAIKSYTKK 307
+ KFW + PV+S QV VN +L V P + ++ L L EFTLG+++P I+ I SY
Sbjct: 132 IVKKFWEVGEPVISAQVYQQVNNELLKVTPPF-LNGLRLTEFTLGSRSPFIEGI-SYISM 189
Query: 308 GDGVVEMDWTVSFTP----NDESNMTPKEAKNKINPKIALGVTIG-KGFVSKSLPVLMED 362
+ ++ V+F P D N ++KN N KI L +G + + +LP+L+++
Sbjct: 190 DGNTLAIEIDVAFVPLEISRDVVNYLENDSKN-WNSKIQLSARVGTRNGIGINLPILVKE 248
Query: 363 INVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPGLKTFV 422
+ G + IK V V +++ P DF L P+ +DIM +PGL ++
Sbjct: 249 LFFKGRVRVVANLFSKNVFIKDVEVCLMDSPEFDFTLVPL----KMVDIMD-VPGLSRWI 303
Query: 423 KTMINSNVGPMLYAPNQLDIDVEEIMAAQSQDAIGVVAVTIDSASDLKTSDI-LSTSVDP 481
+ +INS + + P+ + IDV+ I + + IGVV + I S + + + +D
Sbjct: 304 RGIINSTLATTIVNPSSITIDVDHISKDKGK-TIGVVCLQILSLENEDEERLTVEIDIDG 362
Query: 482 YIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLDQKLNLT 529
+++ T G G + + +NE Y+ + ++D ++ L+
Sbjct: 363 RVEYQTRYG--GGKEIV------------YNEHFYIPIENVDSRIGLS 396
>At1g20080 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 535
Score = 116 bits (291), Expect = 2e-25
Identities = 91/311 (29%), Positives = 139/311 (44%), Gaps = 33/311 (10%)
Query: 244 WLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTIDAIKS 303
WLN + W + + K P +A P Y ID++ TLG+ P+ +K
Sbjct: 71 WLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLTLGSLPPSFQGMKV 130
Query: 304 YTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLPVLMEDI 363
Y D + M+ +V + NP I + + K F K+ V + D+
Sbjct: 131 YA-TDDKEIIMELSVKWAG---------------NPNI---IVVAKAFGLKA-TVQVIDL 170
Query: 364 NVAGTAHITLK-FGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPGLKTFV 422
V T ITLK FP + VS+++ P +DF LK +G D + +PGL FV
Sbjct: 171 QVYATPRITLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGADVMA------IPGLYRFV 224
Query: 423 KTMINSNVGPMLYAPNQLDIDVEEIMAAQSQDAIGVVAVTIDSASDLKTSDILSTSVDPY 482
+ +I V M P L++ + + A + +G+++V + A LK D+L S DPY
Sbjct: 225 QEIIKDQVANMYLWPKTLNVQIMDPSKAMKK-PVGLLSVKVIKAIKLKKKDLLGGS-DPY 282
Query: 483 IKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLD-QKLNLTCYDFNDVRKDAL 541
+K T + + +T VK NP WNE L+V + Q+L L YD+ V K
Sbjct: 283 VKLTLSGDKVPGK---KTVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDK 339
Query: 542 IGSFDIDLSEL 552
IG I L +L
Sbjct: 340 IGMNVIQLKDL 350
Score = 63.2 bits (152), Expect = 3e-09
Identities = 38/146 (26%), Positives = 72/146 (49%), Gaps = 9/146 (6%)
Query: 1056 LKEAIEDPTTISFKNGAQVKIRLLFNPTAIELPLSETVLDTGILDTTFISADDVPSHDRN 1115
++E I+D + + ++++ A++ P+ G+L I A + D
Sbjct: 224 VQEIIKDQVANMYLWPKTLNVQIMDPSKAMKKPV-------GLLSVKVIKAIKLKKKDLL 276
Query: 1116 GKSDPMIIVRIDGEKIFQSAVVKK--TLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGS 1173
G SDP + + + G+K+ V K LNP WNE+ L V ++ + VYDW++ G
Sbjct: 277 GGSDPYVKLTLSGDKVPGKKTVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGK 336
Query: 1174 NDLLAETEWDVRDMTPKKEESFTLKL 1199
+D + ++D+TP++ + TL+L
Sbjct: 337 HDKIGMNVIQLKDLTPEEPKLMTLEL 362
>At3g61050 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 510
Score = 105 bits (261), Expect = 7e-22
Identities = 101/385 (26%), Positives = 169/385 (43%), Gaps = 45/385 (11%)
Query: 179 GVVGTCFFSWLLSYLNFSWWSLGFVFFCTASVYRA-EFRRFNRNIRDDMKRITVEETLS- 236
G++ F + + WS + V +A + + RDD+K+I +
Sbjct: 2 GLISGILFGIIFGVALMAGWSRMMTHRSSKRVAKAVDMKLLGSLSRDDLKKICGDNFPQW 61
Query: 237 ---DRTESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGT 293
E WLN LSK W + ++D+V P L P GI +L ++ TLG
Sbjct: 62 ISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPLLEDYRPP-GITSLKFSKLTLGN 120
Query: 294 KAPTIDAIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVS 353
AP I+ I+ + K +G V MD + + +P I LGVT +
Sbjct: 121 VAPKIEGIRVQSFK-EGQVTMDVDLRWGG---------------DPNIVLGVTA----LV 160
Query: 354 KSLPVLMEDINVAGTAHITLKFGDVFPNIKTVSVSML-EP-PLIDFALKPIGGDTLGLDI 411
S+P+ ++D+ V A + + D P I V V++L EP P ID+ LK +GG
Sbjct: 161 ASIPIQLKDLQVFTVARVIFQLADEIPCISAVVVALLAEPKPRIDYTLKAVGGS------ 214
Query: 412 MSFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQDAI---GVVAVTIDSASD 468
++ +PGL + +++ V ML P+++ + + I S + G + VT+ A++
Sbjct: 215 LTAIPGLSDMIDDTVDTIVKDMLQWPHRIVVPIGGIPVDLSDLELKPQGKLIVTVVKATN 274
Query: 469 LKTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLD-QKLN 527
LK +++ S DPY I +T + NP W++T L+ + Q L
Sbjct: 275 LKNKELIGKS-DPYATI-----YIRPVFKYKTKAIENNLNPVWDQTFELIAEDKETQSLT 328
Query: 528 LTCYDFNDVRKDALIGSFDIDLSEL 552
+ +D DV +D +G + LS L
Sbjct: 329 VEVFD-KDVGQDERLGLVKLPLSSL 352
>At5g04220 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 583
Score = 94.4 bits (233), Expect = 1e-18
Identities = 81/335 (24%), Positives = 145/335 (43%), Gaps = 37/335 (11%)
Query: 244 WLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTI----- 298
W N F+S W + ++ +V P A + I+++ +LGT PT+
Sbjct: 73 WFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSLGTLPPTVHGKSL 132
Query: 299 ------------DAIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVT 346
+K Y + + + + ++ + N + K +I +++ T
Sbjct: 133 SHLALVISYRCFSGVKFY-ETNEKELLFEPSIKWAGNPNIVLVLKVLSLRIRVQVSESET 191
Query: 347 IGKGFVSKS-------LPVLMEDINVAGTAHITLK-FGDVFPNIKTVSVSMLEPPLIDFA 398
+ + + S L + + D+ + LK FP V VS++E P +DF
Sbjct: 192 VKEWNIGISTAEYLNVLTLQLVDLQFFAIVRVALKPLLPTFPCFGMVVVSLMEKPHVDFG 251
Query: 399 LKPIGGDTLGLDIMSFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQDAIGV 458
LK +GGD + +PGL +V+ I V M + P L+I + + A + +G+
Sbjct: 252 LKVLGGDLMS------IPGLYRYVQETIKRQVSSMYHWPQVLEIPILDSSTASVKKPVGL 305
Query: 459 VAVTIDSASDLKTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLL 518
+ V+I A +L D+L TS DPY+K + + + +TT+K NP WNE L+
Sbjct: 306 LHVSILRARNLLKKDLLGTS-DPYVKLSLTGEKLPAK---KTTIKKRNLNPEWNEHFKLI 361
Query: 519 VNSLD-QKLNLTCYDFNDVRKDALIGSFDIDLSEL 552
V + Q L L +D++ V +G I L ++
Sbjct: 362 VKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKI 396
Score = 66.2 bits (160), Expect = 4e-10
Identities = 40/148 (27%), Positives = 72/148 (48%), Gaps = 7/148 (4%)
Query: 1082 PTAIELPLSETVLDT-----GILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKI--FQS 1134
P +E+P+ ++ + G+L + + A ++ D G SDP + + + GEK+ ++
Sbjct: 284 PQVLEIPILDSSTASVKKPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKT 343
Query: 1135 AVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEES 1194
+ K+ LNP WNE KL V + + ++V+DWD+ G +D L ++ + P + +
Sbjct: 344 TIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGERKE 403
Query: 1195 FTLKLSPQGTVKFKSTFIPNYNRPPVDV 1222
F L L V S R VD+
Sbjct: 404 FNLDLIKNSNVVMDSGDKKKRGRLEVDL 431
>At1g05500 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 528
Score = 92.4 bits (228), Expect = 5e-18
Identities = 93/357 (26%), Positives = 156/357 (43%), Gaps = 41/357 (11%)
Query: 238 RTESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPT 297
R+E WLN L+K W S+ +K +V P L P + +L+ ++ TLGT AP
Sbjct: 32 RSELLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYRPAI-VASLTFSKLTLGTVAPQ 90
Query: 298 IDAIKSYTKKGDGV---VEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSK 354
+ +G+ ++M W NP I LGV K V
Sbjct: 91 FTGVSVIDGDKNGITLELDMQWDG-------------------NPNIVLGV---KTLVGV 128
Query: 355 SLPVLMEDINVAGTAHITLK-FGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMS 413
SLP+ +++I G + + + FP VSVS+ E +DF LK +GGD + +S
Sbjct: 129 SLPIQVKNIGFTGVFRLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGGDISAIPGLS 188
Query: 414 FLPGLKTFVKTM-INSNVGPMLYAPNQLDIDVEEIMAAQSQDAIGVVAVTIDSASDLKTS 472
++++V + + VG + QL I + +G++ V + A +L
Sbjct: 189 EAIEVESYVFILELARQVGNL---SRQLKFFCVSIPSDLELKPVGMLEVKLVQAKNLTNK 245
Query: 473 DILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNE-TKYLLVNSLDQKLNLTCY 531
D++ S DP+ K E R+ ++ NP WNE ++++ ++ Q L + Y
Sbjct: 246 DLVGKS-DPFAKMFIRP---LREKTKRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIY 301
Query: 532 DFNDVRKDALIGSFDIDLSELYQKPAQE---HLSKDLVAR--GKSKGVLNYSIDWFP 583
D V+ LIG I L EL ++ L KDL + K++G ++ + + P
Sbjct: 302 DDEGVQASELIGCAQIRLCELEPGKVKDVWLKLVKDLEIQRDTKNRGEVHLELLYIP 358
Score = 56.2 bits (134), Expect = 4e-07
Identities = 31/117 (26%), Positives = 59/117 (49%), Gaps = 2/117 (1%)
Query: 1085 IELPLSETVLDTGILDTTFISADDVPSHDRNGKSDPM--IIVRIDGEKIFQSAVVKKTLN 1142
+ +P + G+L+ + A ++ + D GKSDP + +R EK +S + LN
Sbjct: 218 VSIPSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKTKRSKTINNDLN 277
Query: 1143 PVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL 1199
P+WNE + V S + V++YD + +++L+ + + ++ P K + LKL
Sbjct: 278 PIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGKVKDVWLKL 334
Score = 54.3 bits (129), Expect = 1e-06
Identities = 34/104 (32%), Positives = 56/104 (53%), Gaps = 7/104 (6%)
Query: 1077 RLLFNPTAIELPLS----ETVLDTGILDTTFISADDVPSHDRNGKSDPMIIVRI--DGEK 1130
R+L N T E S + V+ G+L T ISA+++P D GK+DP +++ + G K
Sbjct: 379 RVLKNDTTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMGKADPYVVLSMKKSGAK 438
Query: 1131 IFQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSN 1174
++ VV +LNPVWN+ V + + ++V+D D G +
Sbjct: 439 -SKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKD 481
>At5g11100 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 574
Score = 86.3 bits (212), Expect = 3e-16
Identities = 91/344 (26%), Positives = 144/344 (41%), Gaps = 52/344 (15%)
Query: 244 WLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTIDAIKS 303
WLN L K W S+ +K +V P L P + +L ++FTLGT AP +
Sbjct: 80 WLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAM-LASLKFSKFTLGTVAPQFTGVSI 138
Query: 304 YTKKG--DGV---VEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLPV 358
+ +G+ +EM W NPKI L V K + SLP+
Sbjct: 139 LESESGPNGITMELEMQWDG-------------------NPKIVLDV---KTLLGVSLPI 176
Query: 359 LMEDINVAGTAHITLK-FGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPG 417
+++I G + K D FP +S S+ E +DF LK IGG+ ++ +PG
Sbjct: 177 EVKNIGFTGVFRLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGE------LTSIPG 230
Query: 418 LKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQD----AIGVVAVTIDSASDLKTSD 473
+ ++ I + + P + + I+ D +G + V + A DL D
Sbjct: 231 ISDAIEETIRDAIEDSITWPVR---KIIPILPGDYSDLELKPVGKLDVKVVQAKDLANKD 287
Query: 474 ILSTSVDPY-IKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLD-QKLNLTCY 531
++ S DPY I F + +T S++ NP WNE +V + Q L + +
Sbjct: 288 MIGKS-DPYAIVFIRPL----PDRTKKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVF 342
Query: 532 DFNDVRKDALIGSFDIDLSELYQKPAQE---HLSKDLVARGKSK 572
D V LIG+ + L+EL ++ L KDL + +K
Sbjct: 343 DDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKLVKDLEIQRDTK 386
Score = 59.7 bits (143), Expect = 3e-08
Identities = 33/105 (31%), Positives = 55/105 (51%), Gaps = 2/105 (1%)
Query: 1097 GILDTTFISADDVPSHDRNGKSDPMIIVRIDG--EKIFQSAVVKKTLNPVWNEKVKLPVP 1154
G LD + A D+ + D GKSDP IV I ++ ++ + +LNP+WNE + V
Sbjct: 271 GKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRTKKTKTISNSLNPIWNEHFEFIVE 330
Query: 1155 SRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL 1199
S + V+V+D + GS+ L+ + + ++ P K + LKL
Sbjct: 331 DVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKL 375
Score = 53.1 bits (126), Expect = 3e-06
Identities = 25/85 (29%), Positives = 49/85 (57%), Gaps = 1/85 (1%)
Query: 1093 VLDTGILDTTFISADDVPSHDRNGKSDPMIIVRID-GEKIFQSAVVKKTLNPVWNEKVKL 1151
V+ G+L T ++A+D+P+ D GK+D +++ + E ++ VV +LNPVWN+
Sbjct: 444 VIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSKTRVVPDSLNPVWNQTFDF 503
Query: 1152 PVPSRSRNKIAVQVYDWDRAGSNDL 1176
V + + ++V+D D+ G + +
Sbjct: 504 VVEDALHDLLTLEVWDHDKFGKDKI 528
>7301181 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 816
Score = 74.7 bits (182), Expect = 1e-12
Identities = 100/553 (18%), Positives = 202/553 (36%), Gaps = 76/553 (13%)
Query: 240 ESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTID 299
E WLN L + W VK+ + P +A Y + + LGT P I
Sbjct: 123 ERCEWLNKILKQVWPNANHFARTLVKETIEPNVALALANYKMHGFRFDRIILGTIPPRIG 182
Query: 300 AIKSYTKKGD-GVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLPV 358
+K Y K D + MD + + + + N K I
Sbjct: 183 GVKIYDKNVDRNEIIMDLDLFYASDCDINFYLGGMKGGI--------------------- 221
Query: 359 LMEDINVAGTAHITLK-FGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPG 417
+D + G + +K P + + + L P IDF L + +D M +PG
Sbjct: 222 --KDFQIHGWVRVVMKPLIRSMPLVGGLQIFFLNNPNIDFNLVGV------IDFMD-MPG 272
Query: 418 LKTFVKTMINSNVGPMLYAPNQLDIDVEE---IMAAQSQDAIGVVAVTIDSASDLKTSDI 474
L ++ +I +G ++ PN+L I + E +A + + G++ + + A DL DI
Sbjct: 273 LSDLLRRIIVEQIGNVMVLPNKLPISLSEEVSAVALKMPEPEGILRIHVVEAKDLMKKDI 332
Query: 475 L---STSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNE-TKYLLVNSLDQKLNLTC 530
DPY + +T + + NP+W+ + + + Q + +
Sbjct: 333 SVLGKGKSDPYAIINV------GAQEFKTQIIDNNVNPKWDYWCEATVFIEMGQFVEIQL 386
Query: 531 YDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKDLVARGKSKGVLNYSIDWFPVMXXXXX 590
D +D +KD +G ID++ + +K + L G+L+ + W+ +
Sbjct: 387 KDSDDSKKDENLGRASIDIASVIKKGV---VDSWLTLEDAKHGLLHVRLQWYKLTADPND 443
Query: 591 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGIMKFTLHKVKYLNKSAALTGFLSPCA 650
++ + ++L K A + P
Sbjct: 444 LQQILLETQLLRVTSMSS---------------AVLSVFIDSARHL-KQARSSSKPDPYL 487
Query: 651 ELFIDGKKVKSYRTLRRINEPSWEETIEILIPXXXXXXXXXXXYDDQMHGKRQLAEYTAS 710
++ +K ++ + R + P WE+ L+ YD + + +YT +
Sbjct: 488 VCSVNKQKQQT-AMIMRDDSPVWEQGFTFLVSNPDNESLNIKIYDQKT--GNDIGQYTYT 544
Query: 711 MEDVI-----ELFEQGQTFVQASPQGEIYITSSWKPVAMAGAFKVHNTVREPLAALRVHL 765
+ ++ E+ +Q ++ P+ ++Y++ S + + G + E +AAL
Sbjct: 545 LSTLLKQFNMEVIQQPFQLQKSGPESKLYMSLSLR-ILKPGEIDKDSDALEQVAAL---T 600
Query: 766 KDATISSDDFSGV 778
+ +++ + D + V
Sbjct: 601 RSSSVKTPDVAAV 613
>At1g53590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 706
Score = 73.6 bits (179), Expect = 2e-12
Identities = 81/329 (24%), Positives = 147/329 (44%), Gaps = 36/329 (10%)
Query: 235 LSDRTESSVWLNSFLSKFWTIYMP-VLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGT 293
LSD +ES W+N + K W I M + SQ++ + P + + +G
Sbjct: 21 LSD-SESVRWMNYAVEKIWPICMEQIASQKILGPIIPWFLEKYRPWTAKKAVIQHLYMGR 79
Query: 294 KAPTIDAIKSYTKK-GDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFV 352
P + I+ + GD + ++ ++F D+ + + L +G G
Sbjct: 80 NPPLLTDIRVLRQSTGDDHLVLELGMNFLAADDMSAILA---------VKLRKRLGFGMW 130
Query: 353 SKSLPVLMEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIM 412
+K + + ++V G I +KF +P + + V EPP +KPI T GLD+
Sbjct: 131 TK---LHLTGMHVEGKVLIGVKFLRRWPFLGRLRVCFAEPPYFQMTVKPI--FTHGLDV- 184
Query: 413 SFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQ--------DAIGVVAVTID 464
+ LPG+ ++ +++ L PN L +D+E+ ++ S+ + + V V +
Sbjct: 185 AVLPGIAGWLDKLLSIAFEQTLVQPNMLVVDMEKFVSPTSENWFFVDEKEPVAHVLVEVF 244
Query: 465 SASDLKTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLDQ 524
ASDLK SD L+ DPY+ KG +G +T ++ T +P+W+E + + + D
Sbjct: 245 EASDLKPSD-LNGLADPYV-----KGKLGAYR-FKTKIQKKTLSPKWHEEFKIPIFTWDS 297
Query: 525 K--LNLTCYDFNDVRKDALIGSFDIDLSE 551
LN+ D D D +G +++ E
Sbjct: 298 PSILNIEVGD-KDRFVDDTLGECSVNIEE 325
>CE18256 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 572
Score = 69.3 bits (168), Expect = 4e-11
Identities = 89/355 (25%), Positives = 141/355 (39%), Gaps = 56/355 (15%)
Query: 239 TESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTI 298
TE WLN + + W + D + PQ+ PG + +G +
Sbjct: 113 TERVEWLNKVIHQLWPYVGEYTKTFMNDFIIPQVKAQMPGM-FKNFKFTKMDMGDIPCRV 171
Query: 299 DAIKSYTKK-GDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLP 357
IK YT G + +D V++ + + ++ G T G
Sbjct: 172 GGIKVYTTNVGRDRIIVDMDVAYAGDADFTVS------------CCGFTGG--------- 210
Query: 358 VLMEDINVAGTAHITLK-FGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLP 416
M +I +G LK P + VS + LE P +DF L G+ M LP
Sbjct: 211 --MNNIQFSGKLRAILKPLLPYPPMVGGVSGTFLEMPKMDFNLT-------GMGEMVELP 261
Query: 417 GLKTFVKTMINSNVGPMLYAPNQL------DIDVEEIMAAQSQDAIGVVAVTIDSASDLK 470
GL ++++INS + + PN++ D+DV ++ + GVV + I A +L+
Sbjct: 262 GLIDAIRSVINSQIAALCVLPNEIVVPLAPDVDVTQLYFPEPD---GVVRLKIIEAKNLE 318
Query: 471 TSD---ILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLD-QKL 526
D I DPY + +G++ +T D NP WNE +V+ D QKL
Sbjct: 319 NRDISFIKKGKSDPYAEIQ-----VGSQ-FFKTRTIDDDLNPIWNEYFEAVVDQADGQKL 372
Query: 527 NLTCYDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKDLVARGKSKGVLNYSIDW 581
+ +D D KD +G +DL +L Q A+ + K G G L+ W
Sbjct: 373 RIELFD-EDQGKDEELGRLSVDL-KLVQ--AKGTIDKWYPLEGCKHGDLHIKATW 423
>At3g14590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 705
Score = 68.2 bits (165), Expect = 1e-10
Identities = 94/389 (24%), Positives = 158/389 (40%), Gaps = 75/389 (19%)
Query: 180 VVGTCFFSWLLSYLNFS---WWSLGFVFFCTASVYRAEFRRFNRNIR-DDMKRITVEETL 235
VV W+LSYLN S ++ L V+ V+ R + ++ ++ K+ L
Sbjct: 9 VVIVLLLLWVLSYLNRSHAVFYVLSLVYLYL--VHERYVMRLRKKLQFEERKQANQRRVL 66
Query: 236 SDRTESSVWLNSFLSKFWTIYMP-VLSQQVKDAVNPQ-LAGVAPGYGIDALSLNEFTLGT 293
SD +ES WLN + + W I M + SQ++ + P L P + L +N F
Sbjct: 67 SD-SESVRWLNHAVERIWPICMEQIASQKILRPIIPWFLDKYRPWTAVLELGMN-FLTAD 124
Query: 294 KAPTIDAIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVS 353
I A+K + G G+ WT
Sbjct: 125 DMSAILAVKLRKRLGFGM----WT------------------------------------ 144
Query: 354 KSLPVLMEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMS 413
+ + ++V G I +KF +P + + V EPP +KPI T GLD+ +
Sbjct: 145 ---KLHLTGMHVEGKVLIGVKFLRRWPFLGRLRVCFAEPPYFQMTVKPI--TTHGLDV-A 198
Query: 414 FLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAAQSQD---------AIGVVAVTID 464
LPG+ ++ +++ L PN L +D+E+ ++ +S D + V +
Sbjct: 199 VLPGIAGWLDKLLSVAFEQTLVEPNMLVVDMEKFVSPESGDNWFFVDEKEPVAHALVEVV 258
Query: 465 SASDLKTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNETKYLLVNSLDQ 524
A D+K SD L+ DPY+ KG +G +T + T P+W E + + + D
Sbjct: 259 EACDVKPSD-LNGLADPYV-----KGQLGAYR-FKTKILWKTLAPKWQEEFKIPICTWDS 311
Query: 525 K--LNLTCYDFNDVRKDALIGSFDIDLSE 551
LN+ D D D +G ++++E
Sbjct: 312 ANILNIEVQD-KDRFSDDSLGDCSVNIAE 339
>SPCP31B10.06 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 1188
Score = 65.1 bits (157), Expect = 8e-10
Identities = 87/381 (22%), Positives = 155/381 (39%), Gaps = 48/381 (12%)
Query: 240 ESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGY--GIDALSLNEFTLGTKA-- 295
ES+ W+N L K W + P + V D + + P + + SL++ + +
Sbjct: 230 ESAEWMNHLLEKVWPLINPEMFSSVADQIEDVMQASIPSFVENVRVASLDQGSHPVRVVS 289
Query: 296 ----PTIDAIKSYTKKGDGVV----------------EMDWTVSFTPNDESNMTPKEAKN 335
P+ +A +S+++K E+ P +++ T A N
Sbjct: 290 IRSLPSGEASESFSEKQASEAEHKDEPEQQRKQFYNFELCLAYHAKPVEDATSTSARASN 349
Query: 336 KINPKIALGVTIGKGFVSKSLPVLMEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLI 395
++ +I I KG V LP+ +E + P +K V+ S++ P +
Sbjct: 350 -LHLRIVFYPGI-KGTVGFPLPIWVEIKGFVARIRFRCELMPEVPFLKNVTFSLMGLPEL 407
Query: 396 DFALKPIGGDTLGLDIMSFLPGLKTFVKTMINSNVGPMLYAPNQLDIDVEEIMAA----Q 451
+ + P+ G++I LP + FV I++ + +P + ID+ + + +
Sbjct: 408 NVSAVPVAEG--GVNIFG-LPLISKFVNDAISAAANEYV-SPKSMTIDLSKTLLGDDIKK 463
Query: 452 SQDAIGVVAVTIDSASDLKTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRW 511
+A+GV+ V I+ A DL D+ S D YI K G VK D NP W
Sbjct: 464 EVNALGVIFVHINRAEDLSKQDVNGLS-DAYITVGFHK--FGKPLYCTRVVKQDL-NPIW 519
Query: 512 NETKYLLV----NSLDQKLNLTCYDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKDLVA 567
NE ++ V +K+++ +D + D ++G I L L Q + H D +
Sbjct: 520 NEYAFIPVFPDQVKAGEKISIELWDSDRFSPDDVVGRTKIGLHLLIQDSGKMHERCDTLT 579
Query: 568 RGKSK-----GVLNYSIDWFP 583
G S+ G + Y I +FP
Sbjct: 580 -GISEDTSLPGRVFYEIGYFP 599
Score = 55.1 bits (131), Expect = 8e-07
Identities = 36/115 (31%), Positives = 63/115 (54%), Gaps = 14/115 (12%)
Query: 1081 NPTAIELPLSETVLDTGI------LDTTFIS---ADDVPSHDRNGKSDPMIIVRID--GE 1129
+P ++ + LS+T+L I L F+ A+D+ D NG SD I V G+
Sbjct: 444 SPKSMTIDLSKTLLGDDIKKEVNALGVIFVHINRAEDLSKQDVNGLSDAYITVGFHKFGK 503
Query: 1130 KIFQSAVVKKTLNPVWNEKVKLPV---PSRSRNKIAVQVYDWDRAGSNDLLAETE 1181
++ + VVK+ LNP+WNE +PV ++ KI+++++D DR +D++ T+
Sbjct: 504 PLYCTRVVKQDLNPIWNEYAFIPVFPDQVKAGEKISIELWDSDRFSPDDVVGRTK 558
>Hs14149680 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 1104
Score = 63.9 bits (154), Expect = 2e-09
Identities = 83/424 (19%), Positives = 172/424 (39%), Gaps = 85/424 (20%)
Query: 199 SLGFVFFCTASV--YRAEFRRFNRNIRDDMKRITVEETLSDRT----------------- 239
S+GFV F A +R R++R + + EE L+ +T
Sbjct: 77 SVGFVLFGLALYLGWRRVRDEKERSLRAARQLLDDEEQLTAKTLYMSHRELPAWVSFPDV 136
Query: 240 ESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPTID 299
E + WLN +++ W + + + + V P + G P + + LG K I
Sbjct: 137 EKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPH--LQTFTFTRVELGEKPLRII 194
Query: 300 AIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLPVL 359
+K + + + +D +S+ + + + V + K F +
Sbjct: 195 GVKVHPGQRKEQILLDLNISYVGD-----------------VQIDVEVKKYFCKAGV--- 234
Query: 360 MEDINVAGTAHITLK--FGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPG 417
+ + + G + L+ GD+ P + VS+ + P +D + G+ + +PG
Sbjct: 235 -KGMQLHGVLRVILEPLIGDL-PFVGAVSMFFIRRPTLDI-------NWTGMTNLLDIPG 285
Query: 418 LKTFVKTMINSNVGPMLYAPNQLDI----DVEEIMAAQSQDAIGVVAVTIDSASDLKTSD 473
L + TMI ++ L PN+L + D++++ +S G++ + + +A L
Sbjct: 286 LSSLSDTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGL---- 341
Query: 474 ILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTR-----------NPRWNETKYLLVNSL 522
+S D Y+ KG+I ++D V+ T+ NP+W ET ++V+ +
Sbjct: 342 ---SSKDKYV-----KGLIEGKSDPYALVRLGTQTFCSRVIDEELNPQWGETYEVMVHEV 393
Query: 523 -DQKLNLTCYDFNDVRKDALIGSFDIDLSELYQKPAQEHLSKDLVARGKSKGVLNYSIDW 581
Q++ + +D D KD +G +D+ ++ Q + D +G ++ ++W
Sbjct: 394 PGQEIEVEVFD-KDPDKDDFLGRMKLDVGKVLQASVLD----DWFPLQGGQGQVHLRLEW 448
Query: 582 FPVM 585
++
Sbjct: 449 LSLL 452
>Hs5901958 [T] KOG2059 Ras GTPase-activating protein
Length = 826
Score = 62.8 bits (151), Expect = 4e-09
Identities = 32/103 (31%), Positives = 54/103 (52%), Gaps = 1/103 (0%)
Query: 1099 LDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSRSR 1158
L + + A D+ DRNG SDP + VR G + ++++VKK+ P WNE + + +
Sbjct: 158 LRCSVLEARDLAPKDRNGTSDPFVRVRYKG-RTRETSIVKKSCYPRWNETFEFELQEGAM 216
Query: 1159 NKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKLSP 1201
+ V+ +DWD ND L + DV+ + ++E +L P
Sbjct: 217 EALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQP 259
>Hs21362014 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 692
Score = 62.0 bits (149), Expect = 7e-09
Identities = 36/116 (31%), Positives = 60/116 (51%), Gaps = 7/116 (6%)
Query: 1086 ELPLSETVLDTGI--LDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNP 1143
E+PL+ D G+ LD T + + DR G SDP + +I G+++F+S ++ K LNP
Sbjct: 31 EVPLA----DPGMYQLDITLRRGQSLAARDRGGTSDPYVKFKIGGKEVFRSKIIHKNLNP 86
Query: 1144 VWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL 1199
VW EK + V R + ++V+D+D +D + D+ + + TL L
Sbjct: 87 VWEEKACILV-DHLREPLYIKVFDYDFGLQDDFMGSAFLDLTQLELNRPTDVTLTL 141
Score = 52.4 bits (124), Expect = 5e-06
Identities = 28/103 (27%), Positives = 51/103 (49%), Gaps = 1/103 (0%)
Query: 1097 GILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSR 1156
GI+ T I D+ + D NG SDP + R+ +K ++S ++ KTLNP W E+ +
Sbjct: 203 GIVSITLIEGRDLKAMDSNGLSDPYVKFRLGHQK-YKSKIMPKTLNPQWREQFDFHLYEE 261
Query: 1157 SRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL 1199
I + +D D +D + + D+ ++ ++ L+L
Sbjct: 262 RGGVIDITAWDKDAGKRDDFIGRCQVDLSALSREQTHKLELQL 304
>Hs14150054 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 590
Score = 59.7 bits (143), Expect = 3e-08
Identities = 33/84 (39%), Positives = 51/84 (60%), Gaps = 4/84 (4%)
Query: 1104 ISADDVPSHDRNGKSDPMIIVRI--DGEKIFQSAVVKKTLNPVWNEKVKLPVP--SRSRN 1159
+ A D+P+ D NG SDP + + + D +K FQ+ V +KTLNPV+NE + VP ++
Sbjct: 321 LQALDLPAKDSNGFSDPYVKIYLLPDRKKKFQTKVHRKTLNPVFNETFQFSVPLAELAQR 380
Query: 1160 KIAVQVYDWDRAGSNDLLAETEWD 1183
K+ VYD+DR +DL+ + D
Sbjct: 381 KLHFSVYDFDRFSRHDLIGQVVLD 404
>At2g20990 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 488
Score = 59.7 bits (143), Expect = 3e-08
Identities = 30/105 (28%), Positives = 55/105 (51%), Gaps = 2/105 (1%)
Query: 1097 GILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKI--FQSAVVKKTLNPVWNEKVKLPVP 1154
GI+ + A + D G +DP + +++ +KI ++ V K LNP WNE+ K V
Sbjct: 242 GIVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPSKKTTVKHKNLNPEWNEEFKFSVR 301
Query: 1155 SRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL 1199
+ VYDW++ G+ + + +++M P + ++FTL+L
Sbjct: 302 DPQTQVLEFSVYDWEQVGNPEKMGMNVLALKEMVPDEHKAFTLEL 346
>Hs22045984 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 717
Score = 59.3 bits (142), Expect = 4e-08
Identities = 46/152 (30%), Positives = 74/152 (48%), Gaps = 21/152 (13%)
Query: 1082 PTAIELPLSETV--------LDTGILDTTFISADDVPSHDR------NGKSDPMIIVRID 1127
P I +PL V + G+L FI A D+ D GKSDP I+R+
Sbjct: 158 PNRITVPLVSEVQIAQLRFPVPKGVLRIHFIEAQDLQGKDTYLKGLVKGKSDPYGIIRV- 216
Query: 1128 GEKIFQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDM 1187
G +IFQS V+K+ L+P WNE + V ++ ++++D D +D L D+ ++
Sbjct: 217 GNQIFQSRVIKENLSPKWNEVYEALVYEHPGQELEIELFDED-PDKDDFLGSLMIDLIEV 275
Query: 1188 TPKK--EESFTLKLSPQGTVKFK---STFIPN 1214
++ +E FTL P+G + + T +PN
Sbjct: 276 EKERLLDEWFTLDEVPKGKLHLRLEWLTLMPN 307
>At4g21160_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 162
Score = 58.9 bits (141), Expect = 6e-08
Identities = 31/91 (34%), Positives = 54/91 (59%), Gaps = 3/91 (3%)
Query: 1097 GILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSR 1156
G+L T ++ D SDP +++ + G++ QS VVK LNPVWNE++ L VP
Sbjct: 6 GLLKVTIKKGTNMAIRDMMS-SDPYVVLTL-GQQKAQSTVVKSNLNPVWNEELMLSVP-H 62
Query: 1157 SRNKIAVQVYDWDRAGSNDLLAETEWDVRDM 1187
+ + +QV+D+D ++D++ E E D++ +
Sbjct: 63 NYGSVKLQVFDYDTFSADDIMGEAEIDIQPL 93
>At1g50260 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 778
Score = 58.5 bits (140), Expect = 8e-08
Identities = 62/327 (18%), Positives = 135/327 (40%), Gaps = 33/327 (10%)
Query: 238 RTESSVWLNSFLSKFWTIYMPVLSQQVKDAVNPQLAGVAPGYGIDALSLNEFTLGTKAPT 297
R ES W+N L K W +Y + + + P + + + + + +F+LG + +
Sbjct: 188 RKESVEWVNMVLGKLWKVYRAGIENWLVGLLQPVIDDLKKPDYVQRVEIKQFSLGDEPLS 247
Query: 298 IDAIKSYTKKGDGVVEMDWTVSFTPNDESNMTPKEAKNKINPKIALGVTIGKGFVSKSLP 357
+ ++ T + ++ + +T ++ L +++ G + +P
Sbjct: 248 VRNVERKTSRRANDLQYQIGLRYTG---------------GARMLLMLSLKFGVIPIVVP 292
Query: 358 VLMEDINVAGTAHITLKFGDVFPNIKTVSVSMLEPPLIDFALKPIGGDTLGLDIMSFLPG 417
V + D ++ G + L+ P + VS S + P + F L L +S+
Sbjct: 293 VGVRDFDIDGELWVKLRLIPTQPWVGAVSCSFVSLPKVTFQLAAFRLFNLMGMRLSWSSD 352
Query: 418 LKTFVKTMINSNVGPMLYAPNQLDID----------VEEIMAAQSQ----DAIGVVAVTI 463
F+ ++ ++ + P ++ +D +E++ + + Q D +G ++VT+
Sbjct: 353 PFPFLTKLLTVDLPRLFVRPKKIVLDFQKGKAVGPVLEDLKSGEMQEGNKDFVGELSVTL 412
Query: 464 DSASDLKTSDILSTSVDPYIKFTTEKGIIGNENDLRTTVKSDTRNPRWNET-KYLLVNSL 522
A L+ + DPY +I ++ + +TTV P WN+ ++L+ N
Sbjct: 413 VDAQKLRY--MFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPR 470
Query: 523 DQKLNLTCYDFNDVRKDALIGSFDIDL 549
+Q L + D D IG+ ++DL
Sbjct: 471 EQVLQIEVNDRLGF-ADMAIGTGEVDL 496
>CE26302 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 372
Score = 57.8 bits (138), Expect = 1e-07
Identities = 40/139 (28%), Positives = 69/139 (48%), Gaps = 9/139 (6%)
Query: 1095 DTGILDTTFISADDVPSHDRNGKSDPMIIVRI--DGEKIFQSAVVKKTLNPVWNEK--VK 1150
D L I A+++P+ D G SDP + + + D +K Q+ V +K+LNPV+NE K
Sbjct: 28 DKNSLTVVIIQAEELPAMDLGGTSDPYVKLFLLPDKKKKLQTKVQRKSLNPVFNESFTFK 87
Query: 1151 LPVPSRSRNKIAVQVYDWDRAGSNDLLAETEWDVR--DMTPKKEESFTLKLSPQ---GTV 1205
+P + + V+D+DR G +D + + + D+ E + ++ P+ G V
Sbjct: 88 IPFNEIGGQTLVLNVFDFDRFGKHDQIGQISIPLGKVDLAATLERTDLIESPPENRLGEV 147
Query: 1206 KFKSTFIPNYNRPPVDVSE 1224
++PN N+ V V E
Sbjct: 148 CLALRYVPNKNKLSVVVME 166
>Hs21361472 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4
Length = 955
Score = 56.2 bits (134), Expect = 4e-07
Identities = 42/138 (30%), Positives = 65/138 (46%), Gaps = 16/138 (11%)
Query: 458 VVAVTIDSASDLKTSDILSTSVDPYIKFTTEKGIIGNEND----LRTTVKSDTRNPRWNE 513
++ V + S DL DI S DPY+K + + +EN ++T T NP+WNE
Sbjct: 21 ILRVKVVSGIDLAKKDIFGAS-DPYVKLSL---YVADENRELALVQTKTIKKTLNPKWNE 76
Query: 514 TKYLLVNSLDQKLNLTCYDFNDVRKDALIGSFDIDLSEL-YQKPAQE--HLSKDLVARGK 570
Y VN + +L +D N + +D +G D+ LS L + P E + KD + R +
Sbjct: 77 EFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPR 136
Query: 571 S-----KGVLNYSIDWFP 583
S KG L + + P
Sbjct: 137 SHKSRVKGFLRLKMAYMP 154
>Hs20552506 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 449
Score = 55.8 bits (133), Expect = 5e-07
Identities = 33/90 (36%), Positives = 51/90 (56%), Gaps = 4/90 (4%)
Query: 1098 ILDTTFISADDVPSHDRNGKSDPMIIVRI--DGEKIFQSAVVKKTLNPVWNEKVKLPV-- 1153
+L I A D+P+ D G SDP + + + D +K FQ+ V +KTLNP+++E + PV
Sbjct: 304 LLVVKIIKALDLPAKDFTGTSDPYVKMYLLPDRKKKFQTRVHRKTLNPLFDETFQFPVAY 363
Query: 1154 PSRSRNKIAVQVYDWDRAGSNDLLAETEWD 1183
S K+ VYD+DR +D++ E D
Sbjct: 364 DQLSNRKLHFSVYDFDRFSRHDMIGEVILD 393
>Hs18584396 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 562
Score = 55.8 bits (133), Expect = 5e-07
Identities = 31/94 (32%), Positives = 54/94 (56%), Gaps = 2/94 (2%)
Query: 1113 DRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQVYDWDRAG 1172
DR G SDP + +++G+ +++S V+ K LNPVW+E V LP+ S + K+ V+VYD D
Sbjct: 357 DRCGTSDPYVKFKLNGKTLYKSKVIYKNLNPVWDEIVVLPIQSLDQ-KLRVKVYDRDLT- 414
Query: 1173 SNDLLAETEWDVRDMTPKKEESFTLKLSPQGTVK 1206
++D + + D+ + LKL +++
Sbjct: 415 TSDFMGSAFVILSDLELNRTTEHILKLEDPNSLE 448
>At4g05330_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 165
Score = 55.8 bits (133), Expect = 5e-07
Identities = 28/91 (30%), Positives = 53/91 (57%), Gaps = 3/91 (3%)
Query: 1097 GILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSR 1156
G+L T ++ D SDP +++ + +K+ Q+ V+ LNPVWN+++ L VP
Sbjct: 9 GLLKVTIKKGTNLAIRDMMS-SDPYVVLNLGKQKL-QTTVMNSNLNPVWNQELMLSVP-E 65
Query: 1157 SRNKIAVQVYDWDRAGSNDLLAETEWDVRDM 1187
S + +QVYD+D ++D++ E + D++ +
Sbjct: 66 SYGPVKLQVYDYDTFSADDIMGEADIDIQPL 96
>Hs5032139 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 422
Score = 55.1 bits (131), Expect = 8e-07
Identities = 43/140 (30%), Positives = 69/140 (48%), Gaps = 26/140 (18%)
Query: 1104 ISADDVPSHDRNGKSDPMIIVRI--DGEKIFQSAVVKKTLNPVWNEK--VKLPVPSRSRN 1159
I A ++P+ D G SDP + V + D +K F++ V +KTLNPV+NE+ K+P
Sbjct: 164 IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGK 223
Query: 1160 KIAVQVYDWDRAGSNDLLAE--------------TEWDVRDM-TPKKEESFTLKLSPQGT 1204
+ + VYD+DR +D++ E EW RD+ + +KEE L G
Sbjct: 224 TLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEW--RDLQSAEKEEQEKL-----GD 276
Query: 1205 VKFKSTFIPNYNRPPVDVSE 1224
+ F ++P + V + E
Sbjct: 277 ICFSLRYVPTAGKLTVVILE 296
>Hs4758856 [M] KOG1326 Membrane-associated protein FER-1 and related ferlins
contain multiple C2 domains
Length = 1230
Score = 55.1 bits (131), Expect = 8e-07
Identities = 28/84 (33%), Positives = 45/84 (53%), Gaps = 1/84 (1%)
Query: 1104 ISADDVPSHDRNGKSDPMIIVRIDGEKIFQSA-VVKKTLNPVWNEKVKLPVPSRSRNKIA 1162
+ A D+ D NGK+DP I +R+ I + K LNPV+ + + + +
Sbjct: 732 VRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLT 791
Query: 1163 VQVYDWDRAGSNDLLAETEWDVRD 1186
V VYDWD G++DL+ ET+ D+ +
Sbjct: 792 VAVYDWDLVGTDDLIGETKIDLEN 815
>7302558 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 983
Score = 55.1 bits (131), Expect = 8e-07
Identities = 28/95 (29%), Positives = 51/95 (53%), Gaps = 1/95 (1%)
Query: 1105 SADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSRSRNKIAVQ 1164
S D+ + D+NG SDP + ++ G + +S + + LNPVW+E +P+ + I V+
Sbjct: 261 SGSDLVAMDKNGLSDPYVKFKVGGRLLHKSRTIHRDLNPVWDEVFIVPIEDPFQ-PIIVK 319
Query: 1165 VYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKL 1199
V+D+D +D + + D+ + K E L+L
Sbjct: 320 VFDYDWGLQDDFMGSAKLDLTQLELGKAEDIHLQL 354
>7295906 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 474
Score = 52.8 bits (125), Expect = 4e-06
Identities = 41/143 (28%), Positives = 67/143 (46%), Gaps = 20/143 (13%)
Query: 1099 LDTTFISADDVPSHDRNGKSDPMIIVRI--DGEKIFQSAVVKKTLNPVWNEKV---KLPV 1153
L T I A+++P+ D G SDP + V + D +K F++ V +KTL+PV+NE LP
Sbjct: 209 LAVTVIQAEELPALDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLSPVFNETFTFKSLPY 268
Query: 1154 PSRSRNKIAVQVYDWDRAGSNDL------------LAETEWDVRDMTPKKEESFTLKLSP 1201
+ ++D+DR +D LA+T + RD+ + E KL
Sbjct: 269 ADAMNKTLVFAIFDFDRFSKHDQIGEVKVPLCTIDLAQTIEEWRDLVSVEGEGGQEKL-- 326
Query: 1202 QGTVKFKSTFIPNYNRPPVDVSE 1224
G + F ++P + V + E
Sbjct: 327 -GDICFSLRYVPTAGKLTVVILE 348
>Hs4506067 [T] KOG0696 Serine/threonine protein kinase
Length = 672
Score = 52.4 bits (124), Expect = 5e-06
Identities = 34/120 (28%), Positives = 58/120 (48%), Gaps = 6/120 (5%)
Query: 1099 LDTTFISADDVPSHDRNGKSDPMIIVRI----DGEKIFQSAVVKKTLNPVWNEKVKLPV- 1153
L T A ++ D NG SDP + +++ E ++ ++ TLNP WNE +
Sbjct: 173 LHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLK 232
Query: 1154 PSRSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKLSPQGTVKFKSTFIP 1213
PS +++V+++DWDR ND + + V ++ K S KL Q ++ + IP
Sbjct: 233 PSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELM-KMPASGWYKLLNQEEGEYYNVPIP 291
>CE27898 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 890
Score = 52.4 bits (124), Expect = 5e-06
Identities = 32/123 (26%), Positives = 61/123 (49%), Gaps = 6/123 (4%)
Query: 1096 TGILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPS 1155
T +++ + +D+P D +G SDP + R +++S + K LNP W+E+ ++ V
Sbjct: 142 TLLMEVRLKNGEDLPVKDASGSSDPYVKFRYKDNIVYKSGTIFKNLNPSWDEEFQMIVDD 201
Query: 1156 RSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKLS-----PQGTVKFKST 1210
+ I ++V+D+DR ++D + E D+ + F ++L+ P G V T
Sbjct: 202 VT-CPIRLEVFDFDRFCTDDFMGAAEVDMSQVKWCTPTEFHVELTDEVNQPTGRVSVCVT 260
Query: 1211 FIP 1213
P
Sbjct: 261 ITP 263
>CE09100 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 804
Score = 52.4 bits (124), Expect = 5e-06
Identities = 32/123 (26%), Positives = 61/123 (49%), Gaps = 6/123 (4%)
Query: 1096 TGILDTTFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPS 1155
T +++ + +D+P D +G SDP + R +++S + K LNP W+E+ ++ V
Sbjct: 56 TLLMEVRLKNGEDLPVKDASGSSDPYVKFRYKDNIVYKSGTIFKNLNPSWDEEFQMIVDD 115
Query: 1156 RSRNKIAVQVYDWDRAGSNDLLAETEWDVRDMTPKKEESFTLKLS-----PQGTVKFKST 1210
+ I ++V+D+DR ++D + E D+ + F ++L+ P G V T
Sbjct: 116 VT-CPIRLEVFDFDRFCTDDFMGAAEVDMSQVKWCTPTEFHVELTDEVNQPTGRVSVCVT 174
Query: 1211 FIP 1213
P
Sbjct: 175 ITP 177
>SPAC11G7.02 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4
Length = 767
Score = 51.6 bits (122), Expect = 9e-06
Identities = 36/111 (32%), Positives = 55/111 (49%), Gaps = 9/111 (8%)
Query: 1102 TFISADDVPSHDRNGKSDPMIIVRIDGEKIFQSAVVKKTLNPVWNEKVKLPVPSRSRNKI 1161
T ++AD + D DP ++ +DGE+ + +KKTLNP WNE ++ V S I
Sbjct: 13 TIVAADGLYKRDVFRFPDPFAVLTVDGEQTHTTTAIKKTLNPYWNETFEVNVTDNS--TI 70
Query: 1162 AVQVYDWDR-----AGSNDLLAETEWDVRDMTPKKEESFT--LKLSPQGTV 1205
A+QV+D + G ++ DV D+ +E T LK S + TV
Sbjct: 71 AIQVFDQKKFKKKGQGFLGVINLRVGDVLDLAIGGDEMLTRDLKKSNENTV 121
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,984,148
Number of Sequences: 60738
Number of extensions: 3353686
Number of successful extensions: 9131
Number of sequences better than 1.0e-05: 37
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 32
Number of HSP's that attempted gapping in prelim test: 8895
Number of HSP's gapped (non-prelim): 172
length of query: 1490
length of database: 30,389,216
effective HSP length: 119
effective length of query: 1371
effective length of database: 23,161,394
effective search space: 31754271174
effective search space used: 31754271174
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)