ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1212 good U KOG2069 Intracellular trafficking, secretion, and vesicular transport Golgi transport complex subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1212 431597 430344 -418
(418 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML071c [U] KOG2069 Golgi transport complex subunit 204 2e-52
Hs21166361 [U] KOG2069 Golgi transport complex subunit 74 5e-13
7297851 [U] KOG2069 Golgi transport complex subunit 59 1e-08
SPBC11B10.03 [U] KOG2069 Golgi transport complex subunit 55 2e-07
At5g11980 [U] KOG2069 Golgi transport complex subunit 53 1e-06
>YML071c [U] KOG2069 Golgi transport complex subunit
Length = 607
Score = 204 bits (520), Expect = 2e-52
Identities = 127/355 (35%), Positives = 198/355 (55%), Gaps = 36/355 (10%)
Query: 1 MESLVGEIIETDDKR-DIDFAIGILQGK-ESYDDYFTSEPLPGSXXXXXXXXXXXXSKLE 58
+ SL+ + + + KR +DF ILQ + Y+ YF+S +PGS S L+
Sbjct: 5 LNSLISDDLTEEQKRLSLDFLQDILQSNTKDYESYFSSRAVPGSITEDIAEIDAELSALD 64
Query: 59 RKLKSELIGEKDTLLNILLSSSWSDTLADISEQIEQLWEL----NKDGINGXXXXXXXXX 114
RK++ L+ ++ +L + L DI++ +EQLWEL NK
Sbjct: 65 RKIRKTLLDNTSQIIGNILENDDRAQLDDIAKSLEQLWELDTNINKAADRNVTNDDINNE 124
Query: 115 XXXXXXXVDIDTDLNDA-------------------FHEALEKLRHLHSTERD------- 148
++ D + ND FH+AL +LR+ ST+ D
Sbjct: 125 SVSIDDFLEDDKEDNDTGRIMTTESNNLARKKKEDEFHKALSRLRNRISTKEDDKDDIRS 184
Query: 149 TSLVTVLNNIEKINGILELPTLISTCIRTGHYQEVLILYSYTKTLEKKFPNFELIKMVVK 208
+LVTVL N++ I ++ELP L TCIRTGHYQE ++LY++T +L +FP ++ V +
Sbjct: 185 DTLVTVLENLDSITDLMELPFLARTCIRTGHYQEAVMLYTHTTSLRSRFPGSTIVDEVCE 244
Query: 209 NIQELISTKMLNGLVRLLATNLTLSSMKKILSYLEALEPFD--TNPSALQQLLLTMRYKF 266
+ IST ML+GLV+LL+TN++++S+KKIL YL ++ PFD TN S L + L MRYKF
Sbjct: 245 KVLNEISTTMLSGLVKLLSTNVSVNSLKKILQYLNSIPPFDGKTNKSLL-SVFLAMRYKF 303
Query: 267 VTEEIDSYRI-VDTTANSVKEMLIKRKIECVREYVYGTISVFNSLFKSESKLIHI 320
+T+EI SY + V+++ S+ EM++KRKIE +RE+VY +++VF F ++ + I
Sbjct: 304 ITDEIASYPLDVESSNESLIEMMVKRKIEVLREHVYMSLNVFLKSFLYDTNDLEI 358
Score = 94.4 bits (233), Expect = 3e-19
Identities = 47/121 (38%), Positives = 77/121 (62%), Gaps = 7/121 (5%)
Query: 305 SVFNSLFKSESKLIHIELLLDDT-----TSKTAHDFETSLALLKFINECTNFLISQLET- 358
S+ N K E ++ +E+ ++ TSK + T+ +L+F+++C +++ L
Sbjct: 484 SIDNKAEKEEEEINKVEVTPEEPSKKIRTSKRENKIPTNAVMLQFVDKCITYVLKDLTRG 543
Query: 359 -HKSHLTESVCLQLVYCSFRLADANQNFHHLFMNKLLESNLFTKDALTSAITKRVELATR 417
+ L++SVCLQLVYCSFRL D N+N+HHLF+ K+ +++LFT + L AI KR ELA++
Sbjct: 544 LNSIKLSDSVCLQLVYCSFRLCDLNRNYHHLFLKKINDTSLFTTEQLARAIDKRAELASK 603
Query: 418 Y 418
Y
Sbjct: 604 Y 604
>Hs21166361 [U] KOG2069 Golgi transport complex subunit
Length = 612
Score = 73.6 bits (179), Expect = 5e-13
Identities = 49/184 (26%), Positives = 93/184 (49%), Gaps = 13/184 (7%)
Query: 136 LEKLRHLHSTERDTSLVTVLNNIEKINGILELPTLISTCIRTGHYQEVLILYSYTKTLEK 195
+++ + S R SL LN +I ILE+P L+ TC+R +Y+E L L +Y + LE+
Sbjct: 129 VKEAEEISSNRRMNSLT--LNRHTEILEILEIPQLMDTCVRNSYYEEALELAAYVRRLER 186
Query: 196 KFPNFELIKMVVKNIQELISTKMLNGLVRLLATNLTLSSMKKILSYLEALEPFDTNPSAL 255
K+ + +I+ +V +++ + ML+ L++ L TN+ L + +++ YL ++ F
Sbjct: 187 KYSSIPVIQGIVNEVRQSMQL-MLSQLIQQLRTNIQLPACLRVIGYLRRMDVFTE----- 240
Query: 256 QQLLLTMRYKFVTEEIDSYR-IVDTTANSVKEMLIKRKIECVREYVYGTISVFNSLFKSE 314
+R KF+ R I+ N I + IE R +++ I+ + ++F E
Sbjct: 241 ----AELRVKFLQARDAWLRSILTAIPNDDPYFHITKTIEASRVHLFDIITQYRAIFSDE 296
Query: 315 SKLI 318
L+
Sbjct: 297 DPLL 300
>7297851 [U] KOG2069 Golgi transport complex subunit
Length = 570
Score = 58.9 bits (141), Expect = 1e-08
Identities = 43/169 (25%), Positives = 83/169 (48%), Gaps = 10/169 (5%)
Query: 146 ERDTSLVTVLNNIEKINGILELPTLISTCIRTGHYQEVLILYSYTKTLEKKFPNFELIKM 205
+R + +T+ N + + +LELP L+ CIR G Y+E L L +Y L + + ++
Sbjct: 125 QRRLNSITLQKNAQLLE-VLELPQLMERCIREGRYEEALELAAYATRLGQHQGHIPVVTS 183
Query: 206 VVKNIQELISTKMLNGLVRLLATNLTLSSMKKILSYLEALEPFDTNPSALQQLLLTMRYK 265
+V+++ E + ML LV L T+L L +I+ YL ++ F N L+ L R
Sbjct: 184 IVRSV-EALWHNMLVQLVAQLRTDLQLPKCLQIVGYLRRMQAFGDNELRLK--FLQARDA 240
Query: 266 FVTEEIDSYRIVDTTANSVKEMLIKRKIECVREYVYGTISVFNSLFKSE 314
++T +++ D + + + IE R ++ I+ + ++F +
Sbjct: 241 WLTSCLEAIPTADA------QQHLSKTIEITRINLFNIITQYRAIFPED 283
>SPBC11B10.03 [U] KOG2069 Golgi transport complex subunit
Length = 400
Score = 55.1 bits (131), Expect = 2e-07
Identities = 51/233 (21%), Positives = 113/233 (47%), Gaps = 31/233 (13%)
Query: 130 DAFHEALEKLRH------LHSTE------RDTSLVT----------VLNNIE-KINGILE 166
DAF++ LE RH LH+ + R + +T +++ ++ +++ ILE
Sbjct: 85 DAFYQLLENSRHDFKELVLHTNQLYEPVKRSQNFLTSISEHYRDAKLMHQVQPQLSSILE 144
Query: 167 LPTLISTCIRTGHYQEVLILYSYTKTLEKKFPNFELIKMVVKNIQELISTKMLNGLVRLL 226
LP L++ CI ++ E L + L+ +F +I+ ++ ++ L+ K+ L+ L
Sbjct: 145 LPELMNACIERNYFSETLEFQALAYRLKDRFGTNSIIQELITQVETLV-VKLTEKLILQL 203
Query: 227 ATNLTLSSMKKILSYLEALEPFDTNPSALQQLLLTMRYKFVTEEIDSYRIVDTTANSVKE 286
L L S+ K+++YL + + L+ + L +K + + + +V + E
Sbjct: 204 QKPLKLYSLIKVVTYLRVTAKL--SEAQLKYVFLYFSWKQLQTSLRN--LVPLLDYNNPE 259
Query: 287 MLIKRKIECVREYVYGTISVFNSLF-KSESKLIHIELLLD--DTTSKTAHDFE 336
+ ++R I+ +R+ + + + S+F +S + ++ +D ++TS +A FE
Sbjct: 260 LYLRRYIQVIRDRAFSLLFQYQSVFGESSNDRLNAAGTVDIPNSTSTSASPFE 312
>At5g11980 [U] KOG2069 Golgi transport complex subunit
Length = 619
Score = 52.8 bits (125), Expect = 1e-06
Identities = 58/278 (20%), Positives = 119/278 (41%), Gaps = 30/278 (10%)
Query: 136 LEKLRHLHSTERDTSLVTVLNNIEKINGILELPTLISTCIRTGHYQEVLILYSYTKTLEK 195
L ++ L T+ L+ ++N + ++ + C+R G++ E L L ++ L
Sbjct: 156 LLEIPQLMDTQSLADLLPGVSNFWMCTYLFKMICVFVRCVRNGNFDEALDLEAFVSKLAT 215
Query: 196 KFPNFELIKMVVKNIQELISTKMLNGLVRLLATNLTLSSMKKILSYLEALEPFDTNPSAL 255
P +I+ + +++ + +L+ L++ L +N+ L +I+ YL + F L
Sbjct: 216 LHPKLPVIQALAAEVRQ-TTQSLLSQLLQKLRSNIQLPECLRIIGYLRRIGVFGEYEMRL 274
Query: 256 QQLLLTMRYKFVTEEIDSYRIVDTTANSVKEMLIKRKIECVREYVYGTISVFNSLFKSES 315
Q L R ++T ++ D + E L K I C R +++ ++ + ++F
Sbjct: 275 Q--FLRCREAWLTGILE-----DLDQKNAYEYL-KGMINCHRMHLFDVVNQYRAIF---- 322
Query: 316 KLIHIELLLDDTTSKTAHDFETSLALLKFINECTNFLISQLETHKSHLTESVCL-----Q 370
D TS + +++ L +++ T+ L L+ +TE L Q
Sbjct: 323 ---------SDDTSGSEENYDGGLLFSWAMHQITSHL-KTLKIMLPKITEGGSLSNILDQ 372
Query: 371 LVYCSFRLADANQNFHHLFMNKLLES--NLFTKDALTS 406
+YC+ L +F L E+ NLF+K+ T+
Sbjct: 373 CMYCAMGLGGVGLDFRGLLPPLFEEAVLNLFSKNMSTA 410
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.134 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,261,577
Number of Sequences: 60738
Number of extensions: 794614
Number of successful extensions: 2404
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2394
Number of HSP's gapped (non-prelim): 8
length of query: 418
length of database: 30,389,216
effective HSP length: 109
effective length of query: 309
effective length of database: 23,768,774
effective search space: 7344551166
effective search space used: 7344551166
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)