ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1248 suspect: %id U KOG3771 Intracellular trafficking, secretion, and vesicular transport Amphiphysin
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1248 442398 443588 397
(397 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPBC21D10.12 [U] KOG3771 Amphiphysin 77 3e-14
YDR388w [U] KOG3771 Amphiphysin 76 8e-14
Hs7705296 [U] KOG3771 Amphiphysin 49 1e-05
>SPBC21D10.12 [U] KOG3771 Amphiphysin
Length = 466
Score = 77.4 bits (189), Expect = 3e-14
Identities = 56/238 (23%), Positives = 103/238 (42%), Gaps = 14/238 (5%)
Query: 15 FSDKVLKTPQQLKCKLYTGPRTKDKFVEDLKSRFEAIHGCSSRLVKDCNKFENNTRALLE 74
F+ + +TPQ L+ K G TKD ED RF+++ + +L +D K+ + LL
Sbjct: 6 FTKALARTPQTLRSKFNVGEITKDPIYEDAGRRFKSLETEAKKLAEDAKKYTDAINGLLN 65
Query: 75 H----FERSIEIF----GNIYDVKFTEDEIRRDFTSLSKRFKII----KDKIEADLPIFE 122
H + IEI+ G D + E E + ++ +K I + + +++ +
Sbjct: 66 HQIGFADACIEIYKPISGRASDPESYEQEGNAEGIEAAEAYKEIVYDLQKNLASEMDVIN 125
Query: 123 HNVIEPIQAFHTTCGNIQNTIDKRDLASYKVDSLRHKLTGLTPQKLRTPGLKYENEKIKI 182
++ P ++ + KRD D RH+ + Q+ + LK E + +
Sbjct: 126 TRIVNPTGELLKIVKDVDKLLLKRDHKQLDYD--RHRSSFKKLQEKKDKSLKDEKKLYEA 183
Query: 183 EGKYDKALKVYEPLNEQLRSELGIFLQMTNKFFQDWFLNHYYITYSFYYNMYTFIGTC 240
E ++++ + YE NE L+ EL + F F YY+ + YY +Y + C
Sbjct: 184 ETAFEQSSQEYEYYNEMLKEELPKLFALAQSFIAPLFQGFYYMQLNVYYVLYEKMSHC 241
>YDR388w [U] KOG3771 Amphiphysin
Length = 482
Score = 76.3 bits (186), Expect = 8e-14
Identities = 54/232 (23%), Positives = 107/232 (45%), Gaps = 14/232 (6%)
Query: 15 FSDKVLKTPQQLKCKLYTGPRTKDKFVEDLKSRFEAIHGCSSRLVKDCNKFENNTRALLE 74
F+ V + PQ + K G +T+D ED + RF+ + + +L ++ ++ +L
Sbjct: 6 FTKAVSRAPQSFRQKFKMGEQTEDPVYEDAERRFQELEQETKKLSEESKRYSTAVNGMLT 65
Query: 75 H---FERSIE-----IFGNIYDVKFTEDEIRRDFTSLSKRFKII----KDKIEADLPIFE 122
H F +S+E I G + D T E S++++ I ++ ++ DL + E
Sbjct: 66 HQIGFAKSMEEIFKPISGKMSDPNATIPEDNPQGIEASEQYRAIVAELQETLKPDLALVE 125
Query: 123 HNVIEPIQAFHTTCGNIQNTIDKRDLASYKVDSLRHKLTGLTPQKLRTPGLKYENEKIKI 182
++ P Q I+ KR+ K+D RH T +K + P K E K
Sbjct: 126 EKIVTPCQELLKIITYIRKMATKRN--HKKLDLDRHLNTYNKHEKKKEPTAKDEERLYKA 183
Query: 183 EGKYDKALKVYEPLNEQLRSELGIFLQMTNKFFQDWFLNHYYITYSFYYNMY 234
+ + + A + Y+ N+ L+++L I + +F + F++ Y++ + +Y +Y
Sbjct: 184 QAQVEVAQQEYDYYNDLLKTQLPILFSLEAEFVKPLFVSFYFMQLNIFYTLY 235
>Hs7705296 [U] KOG3771 Amphiphysin
Length = 564
Score = 49.3 bits (116), Expect = 1e-05
Identities = 62/253 (24%), Positives = 99/253 (38%), Gaps = 15/253 (5%)
Query: 18 KVLKTPQQLKCKLYTGPRTKDKFVEDLKSRFEAIHGCSSRLVKDCNKFENNTRALLEHFE 77
K + +++ KL TKD+ E S F +L KD F + + + E +
Sbjct: 20 KFSRAQEKVLQKLGKAVETKDERFEQSASNFYQQQAEGHKLYKDLKNFLSAVKVMHESSK 79
Query: 78 RSIEIFGNIYDVKFTEDEIRRDFTSLSKRFKIIKDKIEADLPIFEHNVIEPIQAFHTTCG 137
R E IY ++ E + ++ ++ + E L + ++ +
Sbjct: 80 RVSETLQEIYSSEWDGHE---ELKAIVWNNDLLWEDYEEKLA---DQAVRTMEIYVAQFS 133
Query: 138 NIQNTIDKRDLASYKVDSLRHKLTGLTPQKLRTPGLKYENEKIKIEGKYDKALKVYEPLN 197
I+ I KR DS RH L + K K E + K E +++KA V+E LN
Sbjct: 134 EIKERIAKRGRKLVDYDSARHHLEAVQNAK------KDEAKTAKAEEEFNKAQTVFEDLN 187
Query: 198 EQLRSELGIFLQMTNKFFQDWFLNHYYITYSFYYNMYTFIGTCSEVRRIVMETQNSNG-- 255
++L EL I + F N + FY M EV +E Q+SN
Sbjct: 188 QELLEELPILYNSRIGCYVTIFQNISNLRDVFYREMSKLNHNLYEVMS-KLEKQHSNKVF 246
Query: 256 VVDDLSPNSLQML 268
VV LS +S + L
Sbjct: 247 VVKGLSSSSRRSL 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,113,894
Number of Sequences: 60738
Number of extensions: 1055387
Number of successful extensions: 3373
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3370
Number of HSP's gapped (non-prelim): 4
length of query: 397
length of database: 30,389,216
effective HSP length: 108
effective length of query: 289
effective length of database: 23,829,512
effective search space: 6886728968
effective search space used: 6886728968
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)