ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1476 good R KOG2382 General function prediction only Predicted alpha/beta hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1476 530222 529191 -344
(344 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR031w [R] KOG2382 Predicted alpha/beta hydrolase 284 2e-76
SPAC22H12.03 [R] KOG2382 Predicted alpha/beta hydrolase 140 3e-33
7290805 [R] KOG2382 Predicted alpha/beta hydrolase 121 2e-27
CE18906 [R] KOG2382 Predicted alpha/beta hydrolase 114 2e-25
7299497 [R] KOG2382 Predicted alpha/beta hydrolase 92 8e-19
CE17746 [R] KOG2382 Predicted alpha/beta hydrolase 71 3e-12
YGR015c [R] KOG2382 Predicted alpha/beta hydrolase 68 2e-11
At3g52570 [R] KOG2382 Predicted alpha/beta hydrolase 54 4e-07
>YGR031w [R] KOG2382 Predicted alpha/beta hydrolase
Length = 342
Score = 284 bits (726), Expect = 2e-76
Identities = 143/269 (53%), Positives = 185/269 (68%), Gaps = 8/269 (2%)
Query: 83 KSPIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDYIGMAADVER 142
+ PIIILHG+FG+K NNR+I R LNKKL RDV+ LD+RNHG SPH H+Y M+ DV+
Sbjct: 74 RPPIIILHGLFGNKLNNRSIGRNLNKKLGRDVYLLDLRNHGSSPHSSVHNYEVMSEDVKH 133
Query: 143 WIKSRDFEEK--PIIVGHSMGAKTAMSVVLRKPDMCAMLVSMDNAPVATQPQSSFPRYVK 200
+I + PII+GHSMG K AM +VL+ P +C+MLV ++NAPV+ +P + F Y+K
Sbjct: 134 FITKHELNTNGGPIIIGHSMGGKVAMMLVLKNPQLCSMLVCIENAPVSLRPNAEFVEYIK 193
Query: 201 ALLGIIEDP--TMHTAQQAMEKL-KXXXXXXXXRQFLMTVLQKVKDEKTGE---YRFKSR 254
AL+ I+ D T+ T +QA E L + R+FL+T L+KVK + + Y F+ R
Sbjct: 194 ALMEIVNDKGKTIRTLKQADEHLAERIGGNELVRRFLLTALKKVKMDNSSSVSSYTFEER 253
Query: 255 IPLGILNDAIVKGNIANWEFNPWVHRWTGPSLFIRGTQSHFIADEYIQDIGRFFPNFEIR 314
IPL L DAIVKG IA W +P RWT P+LFIR TQSH++ DEY+ IG FFP FE R
Sbjct: 254 IPLATLKDAIVKGEIAAWPLDPARERWTRPALFIRATQSHYVVDEYLPIIGAFFPRFETR 313
Query: 315 DVDAGHWLNTEKPQECADLICDFVERHEE 343
D+DAGHW+N EKP ECA+ I DFVERHE+
Sbjct: 314 DIDAGHWVNAEKPGECAESIVDFVERHED 342
>SPAC22H12.03 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 270
Score = 140 bits (353), Expect = 3e-33
Identities = 83/267 (31%), Positives = 147/267 (54%), Gaps = 11/267 (4%)
Query: 74 DEFSSPYEFKSPIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDY 133
+++S+ P++I HG+ GSK N R++A+ + KL RD++++D R HG SP + Y
Sbjct: 11 EKYSATVAKHPPVLIFHGLLGSKRNWRSLAKKFSCKLDRDIYAIDQRCHGDSPCVAPLSY 70
Query: 134 IGMAADVERWIKSRDFEEKPIIVGHSMGAKTAMSVVLRKPDMCAMLVSMDNAPVATQPQS 193
MA D +++K +K I+GHSMGAKTAM L+ PD LV +DN+P
Sbjct: 71 SAMALDAFQFMKDHKL-DKASIIGHSMGAKTAMVTALKWPDKVEKLVVVDNSPWYQDLPR 129
Query: 194 SFPRYVKALLGIIEDPTMHTAQQAMEKLKXXXXXXXXRQFLMTVLQKVKDEKTGEYRFKS 253
+ Y + ++ I++ + +A + + R FL++ L+K + FK
Sbjct: 130 DYGAYFRKMIQ-IDEANITKYSEADKMMSTVEKDILVRSFLLSNLKK---DSNNSNTFKF 185
Query: 254 RIPLGILNDAI--VKGNIANWEFNPWVHRWTGPSLFIRGTQSHFIADEYIQDIGRFFPNF 311
R+P+ +++ ++ ++G A+ N V + P+L IR ++ FI D + +FFP +
Sbjct: 186 RVPIELISKSLKTIEGFPAS--LNDLV--YDSPTLVIRALKAPFIPDSALPVFKKFFPKY 241
Query: 312 EIRDVDAGHWLNTEKPQECADLICDFV 338
E+ +D GHW++ EKP+E ++ I +F+
Sbjct: 242 ELVSLDCGHWVHFEKPKEFSESIINFL 268
>7290805 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 308
Score = 121 bits (303), Expect = 2e-27
Identities = 84/269 (31%), Positives = 144/269 (53%), Gaps = 17/269 (6%)
Query: 81 EFKSPIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDYIGMAADV 140
E P++ HG+FGSK N R I++ L +K++R V+++D+RNHG SPH H+ M+ D+
Sbjct: 50 ETSPPLLTYHGLFGSKQNWRGISKALVRKVSRKVYAIDVRNHGESPHSSVHNSKAMSEDL 109
Query: 141 ERWIKSRDFEEKPIIVGHSMGAKTAMSVVLRKPDMCAMLVSMDNAPVATQPQSS--FPRY 198
+++ R +GHSMG ++ M + P++ L+ +D +P++ P+S+
Sbjct: 110 RLFMEQRS-HPNAACMGHSMGGRSMMYFARKYPELVERLIVVDISPISV-PRSTGEMTEI 167
Query: 199 VKALLGIIEDPTMHTAQQ---AMEKLKXXXXXXXXRQFLMTVLQKVKDEKTGEYRFKSRI 255
A++ + P+M ++ A EKL F+M L+K D TG + +
Sbjct: 168 FDAMVSLDLSPSMSMSEGRKIAREKLLKATEDETV-DFIMLNLRKNPD--TGAFSWACN- 223
Query: 256 PLGILNDAIVKGN--IANWEFNPWVHRWTGPSLFIRGTQSHFIADEYIQDIGRFFPNFEI 313
+L + + + + +N E P +TGP+ FI GT+S ++ E I + FPN EI
Sbjct: 224 -AHVLREFLTRFDKYQSNLEELP---PYTGPTTFICGTRSPYMRREQWPQIQKMFPNSEI 279
Query: 314 RDVDAGHWLNTEKPQECADLICDFVERHE 342
+DAGH ++ EKPQE ++ +F+ R E
Sbjct: 280 HWLDAGHLVHFEKPQEFLTIVSEFLNRTE 308
>CE18906 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 299
Score = 114 bits (286), Expect = 2e-25
Identities = 80/273 (29%), Positives = 136/273 (49%), Gaps = 20/273 (7%)
Query: 76 FSSP-YEFKSPIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDYI 134
+SSP + SP++I+HG+FG K N ++ + L+KKL V+++D+RNHG SPH Y
Sbjct: 37 YSSPELDRNSPLVIVHGLFGQKQNWNSVGKALHKKLEAPVYAVDVRNHGSSPHTETMSYT 96
Query: 135 GMAADVERWIKSRDFEEKPI---IVGHSMGAKTAMSVVL--RKPDMCAMLVSMDNAPVA- 188
MA D+ +I E K ++GHSMG K M + + + D L+ D +P
Sbjct: 97 EMAEDLVLFIDKVKEETKKTRVNLLGHSMGGKIVMRLAIDSKWSDRIEKLIVEDVSPKGY 156
Query: 189 TQPQSSFPRYVKALLGIIEDPTMHTAQQAMEKLKXXXXXXXXRQFLMTVLQKVKDEKTGE 248
++ F +K + + T ++ ++ L+ RQF++T LQ E G+
Sbjct: 157 SRRHLEFRELIKTMRNV---DLCRTRKEILKDLESAIPDLAMRQFILTNLQP-SSENEGQ 212
Query: 249 YRFKSRI-PLGILNDAIVKGNIANWEFNPWVHRWTGPSLFIRGTQSHFIADEYIQDIGRF 307
+K I + D I+ + V + GP+LF+ G S ++ D++ DI
Sbjct: 213 MEWKININTIDSHVDEILGYTLP-------VGSFRGPTLFLHGANSGYVPDDHKPDIKCL 265
Query: 308 FPNFEIRDV-DAGHWLNTEKPQECADLICDFVE 339
FP + + D+GHW++ EKPQ + + F++
Sbjct: 266 FPQVQFDAIPDSGHWVHAEKPQLFINSVYKFLK 298
>7299497 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 306
Score = 92.4 bits (228), Expect = 8e-19
Identities = 68/274 (24%), Positives = 120/274 (42%), Gaps = 38/274 (13%)
Query: 85 PIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDYIGMAADVERWI 144
PI+++H + S + R +A L++ R V ++D RNHG SP+I H + +AADVE +
Sbjct: 47 PIVVMHDLNLSLESWRQVAVNLSQVGLRQVITVDARNHGLSPYITGHSPMHLAADVEA-L 105
Query: 145 KSRDFEEKPIIVGHSMGAKTAMSVVLRKPDMCAMLVSMDNAPVATQPQSSFPRYVKALLG 204
S K + +GH MG + M++ L +P + ++ +D P R V ++
Sbjct: 106 MSHQRLNKIVALGHGMGGRAMMTLALTQPQLVERVILVDITPAPVPSNFYLTRQVFEMM- 164
Query: 205 IIEDPTMHTAQQAMEKLKXXXXXXXXRQFLMTVLQKVKDEKTGEYRFKSRIPLGILNDAI 264
Q + R F++ + Q V + + R I N
Sbjct: 165 ----------LQVAPSIPSNLSLSEGRTFILPLFQDVVHDASELRRI-------IYNLRK 207
Query: 265 VKGNIANWEFNP------W-------------VHRWTGPSLFIRGTQSHFIADEYIQDIG 305
++ N W NP W + + G L I G+QS F+ I +
Sbjct: 208 MQDNTFGWAVNPQAVLSSWGEMMINYEATLGGLRPYMGEVLLIAGSQSEFVTTTSIAVMQ 267
Query: 306 RFFPNFEIRDVDAGHWLNTEKPQECADLICDFVE 339
R+FPN ++ +DAGH + ++P++ +L+ +F +
Sbjct: 268 RYFPNTVVQILDAGHCVYEDQPEQFVELVVEFTQ 301
>CE17746 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 297
Score = 70.9 bits (172), Expect = 3e-12
Identities = 60/267 (22%), Positives = 125/267 (46%), Gaps = 19/267 (7%)
Query: 84 SPIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDYIGMAADVER- 142
+P+I++ G+FG+K N + + L+++L VF+++ RNHG Y MA D+
Sbjct: 36 TPLILVPGLFGTKENWIQVGKDLSQRLGCMVFAVENRNHGSFSKAASMTYEEMADDLVGF 95
Query: 143 --WIKSRDFEEKPIIVGHSMGAKTAMSVVLRKPDMCAMLVSM---DNAPVATQPQSSFPR 197
W++ E+K + GHSMG K A++ + P+ + + S+ D +P+ + +
Sbjct: 96 IDWVRKITGEDKVNLHGHSMGGK-AVTQLATTPEYSSRIKSLIVEDMSPLGYPLKRA--E 152
Query: 198 YVKALLGIIEDPTMHTAQQAMEKLKXXXXXXXXRQFLMTVLQKVKDEKTGEYRFKSRIPL 257
Y++ + +I + + M +L QF V + ++ G+ + L
Sbjct: 153 YLECIKQMIATDMNKSRSEVMAELGEKVSKVLLYQF---VRGNLGEDVNGKAHWICN--L 207
Query: 258 GILNDAIVKGNIANWEFNPWVHRWTGPSLFIRGTQSHFIADEYIQDIGRFFPNFEIRDVD 317
++++ + + F + GP+LF R S F+ + + + FP + +
Sbjct: 208 NVIDETYIYLLSHDIRFGVF----DGPTLFQRAPGSGFLPAAHKNRVEKMFPMVQFAETA 263
Query: 318 -AGHWLNTEKPQECADLICDFVERHEE 343
+ HW++ + P+ D IC+F+E ++
Sbjct: 264 WSNHWIHADDPKFFVDSICEFLEEPDQ 290
>YGR015c [R] KOG2382 Predicted alpha/beta hydrolase
Length = 328
Score = 68.2 bits (165), Expect = 2e-11
Identities = 39/111 (35%), Positives = 64/111 (57%), Gaps = 1/111 (0%)
Query: 80 YEFKSPIIILHGIFGSKSNNRTIARILNKKLTRDVFSLDMRNHGGSPHIGRHDYIGMAAD 139
+E + II +HG+ GS ++ ++L++KL D+FS+D+RNHG SP +DY + D
Sbjct: 35 FEQRPAIINIHGLLGSHVMFHSLNKLLSRKLDADIFSVDVRNHGISPKAIPYDYTTLTND 94
Query: 140 VERWIKSRDFEEKPI-IVGHSMGAKTAMSVVLRKPDMCAMLVSMDNAPVAT 189
+ +I++ E+PI ++G SMG K A+ L K +S+D P T
Sbjct: 95 LIYFIETHIGLERPIYLLGFSMGGKIALLTTLYKNINIRKCISIDLPPYET 145
>At3g52570 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 343
Score = 53.5 bits (127), Expect = 4e-07
Identities = 32/100 (32%), Positives = 51/100 (51%), Gaps = 10/100 (10%)
Query: 77 SSPYEFKSPIIILHGIFGSKSNNRTIARILNKKLTRDVFS------LDMRNHGGSPHIGR 130
S E +S +ILHG+ GS N R+ +R L L+ S +D+RNHG S +
Sbjct: 47 SGDRESESTALILHGLLGSGRNWRSFSRSLASSLSVSSASDWKMILVDLRNHGRSAEVEG 106
Query: 131 ----HDYIGMAADVERWIKSRDFEEKPIIVGHSMGAKTAM 166
HD + A D+ +K+ + +++GHS+G K A+
Sbjct: 107 LNPPHDLVNSAKDLADLVKASGWNWPDVVIGHSLGGKVAL 146
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,640,892
Number of Sequences: 60738
Number of extensions: 866338
Number of successful extensions: 1887
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1863
Number of HSP's gapped (non-prelim): 11
length of query: 344
length of database: 30,389,216
effective HSP length: 107
effective length of query: 237
effective length of database: 23,890,250
effective search space: 5661989250
effective search space used: 5661989250
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)