ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1521 good T KOG2141 Signal transduction mechanisms Protein involved in high osmolarity signaling pathway
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1521 543019 546171 1051
(1051 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR336c [T] KOG2141 Protein involved in high osmolarity signalin... 595 e-169
SPAC24C9.11 [T] KOG2141 Protein involved in high osmolarity sign... 269 1e-71
7292230 [T] KOG2141 Protein involved in high osmolarity signalin... 179 2e-44
At5g17930 [T] KOG2141 Protein involved in high osmolarity signal... 135 3e-31
Hs22046307 [T] KOG2141 Protein involved in high osmolarity signa... 122 3e-27
CE20298 [T] KOG2141 Protein involved in high osmolarity signalin... 114 6e-25
>YLR336c [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 899
Score = 595 bits (1533), Expect = e-169
Identities = 307/610 (50%), Positives = 418/610 (68%), Gaps = 12/610 (1%)
Query: 445 KQKENIYAAPVIEENKSYVPPSLRRKQLEESDSETNKELKRKVKSALNKLSDSNSAIIVT 504
++KEN Y AP + +SYVPPSLR+K + ++ T E+ +KV S+LNKLSDSN II+T
Sbjct: 297 REKENPYVAPT-QSVESYVPPSLRKKLDDSENNSTLSEISKKVNSSLNKLSDSNITIIIT 355
Query: 505 SLNELYDNYARQYVNDAINNQMIEVVAQKNKLLDTFIMNYAGVAFSIWKLRGTEAGASFI 564
LN LYD+ RQYV +++ ++ +++Q KLLD FIMNYA +A+++ KLRG E GA FI
Sbjct: 356 DLNRLYDSLPRQYVTESLTKGILNIISQNQKLLDGFIMNYAALAYTLSKLRGIEVGAFFI 415
Query: 565 QALVQKFLEIYSEQIQSARNSKSDEPIMLSKETTNLLTLLGYSYNFGLVSSRLIYDIIKL 624
Q V+ FL Y E++++ K + + SK N+ TLL Y YNFG VS RLIYDII++
Sbjct: 416 QKTVEAFLHHYEEEMENIL--KDQQSKISSKICINIATLLSYCYNFGFVSCRLIYDIIRI 473
Query: 625 LIQEPNEYTTELLLRIISVCGPLIRGDDPRALKDIITELLANVKQMK-QTPRLNFLLETL 683
+ +PNE+TTELLLRIIS+ G LIRGDDP AL+DI +ELL N K +K Q+PRL FL++T+
Sbjct: 474 FVADPNEFTTELLLRIISISGQLIRGDDPSALRDIRSELLKNAKNLKEQSPRLRFLMDTM 533
Query: 684 SDLKNNRLKPSVLAANHQTLKKGILGSLRIXXXXXXXXXXXXXXDIKNVDSKGKWWLIGA 743
SDLKNNRLKPS+LA +H LKK + L DIKN+DSKGKWWL+GA
Sbjct: 534 SDLKNNRLKPSILATDHHPLKKNLQSILN--SSSSWEPLQVSLEDIKNIDSKGKWWLVGA 591
Query: 744 SWKGNQETAFDEA-NVKEDRTIANQTNIKLDDDLLSEMIDWNEIAKQQRMNTDVRRAIFV 802
SW+GN E AF+ + N + D + + ++ I ++DDLL ++ DWN IA+QQRMNTD+RRAIF+
Sbjct: 592 SWRGNMENAFEVSINNENDASKSKKSKISIEDDLLDDIPDWNIIARQQRMNTDIRRAIFI 651
Query: 803 SIMSAEDYLDAFAKIEKLNLKSKQSLDISRVLLHCLSNDGTSSGYNPYYSLLAGKLSEHN 862
SIMSA+DYLDAF+K+EKL+LK+KQ L+I R++LHCL D S+GYN YY+L+A K+ E
Sbjct: 652 SIMSAQDYLDAFSKLEKLSLKNKQVLEIPRIVLHCLLADSGSNGYNHYYALVANKICERY 711
Query: 863 HKLLKSFQFLFWEIVKKFELETHSDDEEDTLFSNEDTVDEDTRLQRLAKQGRFFGHLIAE 922
L KSFQFLFW+++KKFE + D E DT ED +D+ +L R++ QGRFFG L+A
Sbjct: 712 SHLSKSFQFLFWDVIKKFE-DKEFDSESDT--DEEDDLDDKEKLLRISNQGRFFGSLLAN 768
Query: 923 GHLKLDAFKHVPLMAGLNSDGXXXXXXXXXXXXXSEGKAAEIKIKRD--GKKTFEYNSDG 980
LKLD FKHVP M GLN++G + K +E K+K D G K Y+ D
Sbjct: 769 DILKLDVFKHVPFMGGLNTEGMLFMEILLFQLFLTVAKKSEKKLKMDESGNKRIIYSDDY 828
Query: 981 LSSLIEKGINIQNKNVILKALRWFISKHFDYIKYISGMKGSKEHDRESRRLSWAVASFKK 1040
L ++ K + +N ILK L+WFI+K F Y +++G KG K DR+ RRL+WA + K
Sbjct: 829 LRDVLTKNVKSENMLFILKGLKWFINKKFRYHNFLAGKKGDKAFDRDERRLAWASKAAKS 888
Query: 1041 LIEEELKGAE 1050
+I++EL+ +
Sbjct: 889 IIDKELENID 898
>SPAC24C9.11 [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 775
Score = 269 bits (688), Expect = 1e-71
Identities = 171/506 (33%), Positives = 271/506 (52%), Gaps = 54/506 (10%)
Query: 447 KENIYAAPVIEENKSYVPPSLRRKQLEESDSETNKELKRKVKSALNKLSDSNSAIIVTSL 506
K + + P YVPPSLR+K + +SE L+RK++ +LNKLS +N + I+ +
Sbjct: 225 KPAVPSLPNANVGSKYVPPSLRKKLGGDKESEDALRLRRKLQGSLNKLSIANISSIIKEI 284
Query: 507 NELYDNYARQYVNDAINNQMIEVVAQKNKLLDTFIMNYAGVAFSIWKLRGTEAGASFIQA 566
LY +R V I N +++ V + +LD + YA +A +++++ G + GA +Q
Sbjct: 285 EVLYMENSRHSVTSTITNLLLQTVMGRESMLDQLAIVYAALATALYRIVGNDFGAHLLQT 344
Query: 567 LVQKFLEIYSEQIQSARNSKSDEPIMLSKETTNLLTLLGYSYNFGLVSSRLIYDIIKLLI 626
LV++FL++Y SK EP+ KET+NL+ YNF LVS L+YD+I+L +
Sbjct: 345 LVERFLQLY--------KSKEKEPLSSHKETSNLIVFFVELYNFQLVSCVLVYDLIRLFL 396
Query: 627 QEPNEYTTELLLRIISVCGPLIRGDDPRALKDIITE---LLANVKQMKQTPRLNFLLETL 683
+ E E+LL+I+ CG +R DDP +L+DI+TE LLA+ + R F++E++
Sbjct: 397 RSLTELNVEMLLKIVLNCGGQLRSDDPTSLQDIVTEMNLLLASADPSTISVRTKFMVESI 456
Query: 684 SDLKNNRLKPSVLAANHQT-------LKKGILGSLRIXXXXXXXXXXXXXXDIKNVDSKG 736
++LK N+ K V A+ Q+ LKK LGSL DI+ +++KG
Sbjct: 457 TNLKENK-KTKVANASAQSKFEAVNQLKK-FLGSLGNRSLNAREPLRVTLEDIEQIETKG 514
Query: 737 KWWLIGASWKGNQETAFDEANVKE-DRTIANQTNIKLDDDLLSEMIDWN----EIAKQQR 791
+WWL+GASW NV D T++ + L D SE + + + AK+ R
Sbjct: 515 RWWLVGASWN----------NVPSGDNTLSTEA---LQDKKKSEELTAHSKILQAAKKLR 561
Query: 792 MNTDVRRAIFVSIMSAEDYLDAFAKIEKLNLKSKQSLDISRVLLHCLSNDGTSSGYNPYY 851
+N+ +R +IFV+++ +EDY+DA+ ++ KL+LK Q +I+ V+LHC+ N+ YNP+Y
Sbjct: 562 LNSTLRTSIFVALVGSEDYIDAWERVLKLHLKRNQLPEIAYVILHCVGNEKL---YNPFY 618
Query: 852 SLLAGKLSEHNHKLLKSFQFLFWEIVKKFELETHSDDEEDTLFSNEDTVDEDTRLQRLAK 911
L+A K H L KSFQF W+ EL+ D EE + ++R+
Sbjct: 619 GLVALKCCTLQHNLKKSFQFSLWDFFN--ELQPDDDSEE-----------REISMRRIVN 665
Query: 912 QGRFFGHLIAEGHLKLDAFKHVPLMA 937
+ + L+ E L KHV MA
Sbjct: 666 LAKLYASLVIEAAQPLTILKHVDFMA 691
>7292230 [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 854
Score = 179 bits (455), Expect = 2e-44
Identities = 132/470 (28%), Positives = 225/470 (47%), Gaps = 45/470 (9%)
Query: 433 ELEGNAPS------STRTKQKENIYAAPVIEE----NKSYVPPSLRRKQL-----EESDS 477
E E NAP + + NI PV E + Y+PP R E +
Sbjct: 286 EQEENAPKIKEDIYGRKRDAEGNILPDPVELEASAAGQKYIPPHQRALMAASAGSSEKQA 345
Query: 478 ETNKELKRKVKSALNKLSDSNSAIIVTSLNELYDNYARQYVNDAINNQMIEVVAQKNKLL 537
E L ++ K LN+LS++N I + ELY +R +N+ + + E + +
Sbjct: 346 EILARLLKQCKGLLNRLSEANLHKIAAGIEELYMKNSRYNMNETLTKLIQEALLGYTRTN 405
Query: 538 DTFIMNYAGVAFSIWKLRGTEAGASFIQALVQKFLEIYSEQIQSARNSKSDEPIMLSKET 597
+ + + + + G+E GA F+Q F+E++ +++ + ++ K+
Sbjct: 406 ERMVQEHMVLLAYLHAQVGSEIGAHFLQT----FVELFDGYVKNIATLEVED-----KQL 456
Query: 598 TNLLTLLGYSYNFGLVSSRLIYDIIKLLIQEPNEYTTELLLRIISVCGPLIRGDDPRALK 657
NL+ +L Y Y F + L+ ++I L + E + E LL I G +R DDP A K
Sbjct: 457 NNLVLVLCYMYLFKIYELNLLMELIGKLSDQLCEKSVECLLLIFQSIGFRLRKDDPLAFK 516
Query: 658 DIITELLANVK----QMKQTPRLNFLLETLSDLKNNRLK--PSVLAANHQTLKKGILGSL 711
++ ++ + + ++K+ PRL F+++ L+ +KNN ++ P + L+K + L
Sbjct: 517 TMMQKVQSQIASAPLELKENPRLRFMVDILNAVKNNNMQKLPQYDPELAENLRKRLKAML 576
Query: 712 RIXXXXXXXXXXXXXXDIKNVDSKGKWWLIGASWKGNQETAFDEANVKEDRTIANQTNIK 771
+ D+ D GKWW++G++W GN + A K+D+ + N
Sbjct: 577 K--NDRYVVTLNITLEDLLRADKVGKWWIVGSAWTGNLDE-MGSAKQKQDKNSSKSANGG 633
Query: 772 LDDDLLSEMIDWNEIAKQQRMNTDVRRAIFVSIMSAEDYLDAFAKIEKLNLKSKQSLDIS 831
D LL E+A++Q+MNT RR IF IMSA DY+DAF KI L+LK +++ ++
Sbjct: 634 FADQLL-------ELARKQQMNTAERRNIFCIIMSAADYVDAFEKILHLSLKDQRA--VA 684
Query: 832 RVLLHCLSNDGTSSGYNPYYSLLAGKLSEHNHKLLKSFQFLFWEIVKKFE 881
V++HC N+ + NPYY+ LA K + N K +FQF W+ + E
Sbjct: 685 YVIIHCALNEKRA---NPYYAHLALKFCQFNRKYQLAFQFASWDRINDIE 731
>At5g17930 [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 707
Score = 135 bits (341), Expect = 3e-31
Identities = 117/385 (30%), Positives = 171/385 (44%), Gaps = 66/385 (17%)
Query: 562 SFIQALVQKFLEIYSEQIQSARNSKSDEPIMLSKETTNLLTLLGYSYNFGLVSSRLIYDI 621
S ++ + + IY ++ Q K D S + LL Y G+ SS LIYD
Sbjct: 295 SNVETITAELATIYRDEYQ-----KED-----SLSLNGISLLLSYLCILGVCSSDLIYDF 344
Query: 622 IKLLIQEPNEYTTELLLRIISVCGPLIRGDDPRALKDIITELLANVKQMK-----QTPRL 676
+ L + + + ++ CG IR DDP A+K I + ++K QT
Sbjct: 345 LMTLGKRLTKVDAFTITTVLDCCGMKIRSDDPLAMKTFIISIQNKANELKTSSDGQTQMN 404
Query: 677 NFL----LETLSDLKNNRLKPSVLAANHQTLKKGILGSLRIXXXXXXXXXXXXXXDIKNV 732
N + LET+S +KNN+L+ + + +KK L LR+ D
Sbjct: 405 NMMMEKMLETISAIKNNKLRAKEDSVQNTRVKKW-LQKLRVEEVLLRGLTWSKLLD---P 460
Query: 733 DSKGKWWLIGASWKGNQETAFDEANVKEDRTIANQTNIKLDDDLLSEMIDWNEIAKQQRM 792
+ KG+WWL G + TA D A + + Q +KL A+ QRM
Sbjct: 461 EKKGQWWLSG-DLVVDSNTAEDIAETMDAEVVEAQKMLKL--------------AEAQRM 505
Query: 793 NTDVRRAIFVSIMSAEDYLDAFAKIEKLNLKSKQSLDISRVLLHCLSNDGTSSGYNPYYS 852
NTD R+AIF IMS+EDY+DAF K+ +L+L KQ +I RVL+ C + +N +Y+
Sbjct: 506 NTDSRKAIFCVIMSSEDYIDAFEKLLRLDLPGKQDREIMRVLVECCLQE---KAFNKFYT 562
Query: 853 LLAGKLSEH--NHKLLKSFQFLFWEIVKKFELETHSDDEEDTLFSNEDTVDEDTRLQRLA 910
+LA KL EH NHK + Q+ W+ K ELE+ S LQR
Sbjct: 563 VLASKLCEHDKNHKF--TLQYCIWDHFK--ELESMS-------------------LQRSM 599
Query: 911 KQGRFFGHLIAEGHLKLDAFKHVPL 935
+F +I +L L K V L
Sbjct: 600 HLAKFVAEIIVTFNLSLAVLKSVDL 624
>Hs22046307 [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 356
Score = 122 bits (306), Expect = 3e-27
Identities = 74/240 (30%), Positives = 122/240 (50%), Gaps = 21/240 (8%)
Query: 648 IRGDDPRALKDIITELLANVK----QMKQTPRLNFLLETLSDLKNNRLK--PSVLAANHQ 701
+R DD +LK++ITE + + R+ F+LET+ LKNN ++ P +
Sbjct: 9 LRKDDALSLKELITEAQTKASGAGSEFQDQTRIRFMLETMLALKNNDMRKIPGYDPEPVE 68
Query: 702 TLKKGILGSLRIXXXXXXXXXXXXXXDIKNVDSKGKWWLIGASWKGNQETAFDEANVKED 761
L+K +R + + + G+WW++G++W G D
Sbjct: 69 KLRKLQRALVRNAGSGSETQLRVSWDSVLSAEQTGRWWIVGSAWSGAPMI---------D 119
Query: 762 RTIANQTNIKLDDDLLSEMIDWNEIAKQQRMNTDVRRAIFVSIMSAEDYLDAFAKIEKLN 821
+ +L + S+++ E+A++QRMNTD+RR IF +IM++ED+LDAF K+ KL
Sbjct: 120 NSHHTHLQKQLVGTVSSKIL---ELARKQRMNTDIRRNIFCTIMTSEDFLDAFEKLLKLG 176
Query: 822 LKSKQSLDISRVLLHCLSNDGTSSGYNPYYSLLAGKLSEHNHKLLKSFQFLFWEIVKKFE 881
LK +Q +I VL+ C + T YNP+Y+ LA K E+ + +FQF W+ + E
Sbjct: 177 LKDQQEREIIHVLMDCCLQEKT---YNPFYAFLASKFCEYERRFQMTFQFSIWDKFRDLE 233
>CE20298 [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 819
Score = 114 bits (286), Expect = 6e-25
Identities = 102/458 (22%), Positives = 201/458 (43%), Gaps = 36/458 (7%)
Query: 433 ELEGNAPSSTRTKQKENIYAAPVIEENKSYVP--PSLRRKQLEESDS-----ETNKELKR 485
E+E +AP ++++ E+IY + + + P+ RK+LEE D+ E + ++R
Sbjct: 276 EIETSAPK--KSEETEDIYGRKINKSTGELIKFDPAAARKKLEELDATSSTGEQKQRVER 333
Query: 486 KVKSALNKLSDSNSAIIVTSLNELYDNYARQYVNDAINNQMIEVVAQKNKLLDTFIMNYA 545
V S +NKLSD+ + EL+ N ++ V A+ + + + +L D+ + Y
Sbjct: 334 AVNSIINKLSDAMLIKSQQGIAELWANSSKNDVKSALYKFLSKALLAPFRLQDSLLTTYG 393
Query: 546 GVAFSIWKLRGTEAGASFIQALVQKFLEIYSEQIQSARNSKSDEPIMLSKETTNLLTLLG 605
+ + E A F++ + ++ S +E M K N +
Sbjct: 394 ALIAMCHTMISNEISAHFVECFMCDLVK-----------SMQEEEEMDDKSMENCVIFTA 442
Query: 606 YSYNFGLVSSRLIYDIIKLLIQEPNEYTTELLLRIISVCGPLIRGDDPRALKDIITELLA 665
+ F ++ ++ +II L + N + +I+ ++ + I E+ +
Sbjct: 443 FLVVFRILQPSILIEIIDKLAENLNLTNLFAIRTLIAYAYKSLKKTSWPLVTQKIDEIHS 502
Query: 666 NV--KQMKQTPRLNFLLETLSDLKNNRLKPSVLAANHQTLK--KGILGSLRIXXXXXXXX 721
K + PR FLLE + LK + K + +K GI
Sbjct: 503 EFEKKPISALPRAKFLLEEVILLKKSPPKAIDYDVVERQVKILHGITKKKGSSASSDSKE 562
Query: 722 XXXXXXDIKNVDSKGKWWLIGASWKGNQETAFDEANVKEDRTIANQTNIKLDDDLLSEMI 781
D+ + + +G+WW+IG++++ ++ + + + A Q K EM+
Sbjct: 563 LSMTLNDLLHAEQRGRWWIIGSAFRLPEQGGY---GLLASASAAGQ---KKTGSFPPEMV 616
Query: 782 DWNEIAKQQRMNTDVRRAIFVSIMSAEDYLDAFAKIEKLNLKSKQSLDISRVLLHCLSND 841
+A + MN++VRR IF S+ SA+D DAF ++ KL LK ++ ++ VL+ + +
Sbjct: 617 S---LATKAGMNSEVRRHIFCSVASADDEDDAFERLLKLQLKGEKERELVHVLIAMMMKE 673
Query: 842 GTSSGYNPYYSLLAGKLSEHNHKLLKSFQFLFWEIVKK 879
T YN +Y+ L + E N + + + QF W+ +++
Sbjct: 674 KT---YNAFYATLLQRFCEFNKRFVITLQFALWDRLRE 708
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.131 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,384,279
Number of Sequences: 60738
Number of extensions: 1720831
Number of successful extensions: 4996
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4960
Number of HSP's gapped (non-prelim): 9
length of query: 1051
length of database: 30,389,216
effective HSP length: 117
effective length of query: 934
effective length of database: 23,282,870
effective search space: 21746200580
effective search space used: 21746200580
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)