ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1644 good L KOG3014 Replication, recombination and repair Protein involved in establishing cohesion between sister chromatids during DNA replication
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1644 587406 586594 -271
(271 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFR027w [L] KOG3014 Protein involved in establishing cohesion be... 228 9e-60
SPBC16A3.11_2 [L] KOG3014 Protein involved in establishing cohes... 84 3e-16
Hs18570262 [L] KOG3014 Protein involved in establishing cohesion... 62 7e-10
Hs16163800_2 [L] KOG3014 Protein involved in establishing cohesi... 59 1e-08
7295257_2 [L] KOG3014 Protein involved in establishing cohesion ... 54 3e-07
>YFR027w [L] KOG3014 Protein involved in establishing cohesion between
sister chromatids during DNA replication
Length = 281
Score = 228 bits (580), Expect = 9e-60
Identities = 113/268 (42%), Positives = 172/268 (64%), Gaps = 12/268 (4%)
Query: 13 IQATLQFKS---TPTLVKCPKCSITYSTNSPSDLVQHKRYHDLHLNGKRWSQSWGDIINR 69
IQ+ LQ + + +VKC KC ++YS+ S D H++YH L L+G++WS +WG I+
Sbjct: 17 IQSKLQVNNGSKSNKIVKCDKCEMSYSSTSIEDRAIHEKYHTLQLHGRKWSPNWGSIVYT 76
Query: 70 VEALKTEKQLKYSMVSEKDKKVMSFQLPSQTLYEEDEYIVMISPKKAN-EVKAALDLMSI 128
L S + + S ++ ++E IV + P K+N EV+A ++M++
Sbjct: 77 ERNHSRTVHLSRSTGTITPLNSSPLKKSSPSITHQEEKIVYVRPDKSNGEVRAMTEIMTL 136
Query: 129 VNEELNAPHDENAFWSEVDKSGKSQGKAFIYVKKNRAVGVITIEYI-----ENTNRGKWM 183
VN ELNAPHDEN W+ + +GKAF+Y++ +RAVG+I IE + + ++RG+WM
Sbjct: 137 VNNELNAPHDENVIWNSTTEE---KGKAFVYIRNDRAVGIIIIENLYGGNGKTSSRGRWM 193
Query: 184 VLDTKAIVPNVVPDVKLGISRIWVCRNQRQHGIATRLLEVARKKSIYGCIVNKWELAWSQ 243
V D++ +V NV PD K+GISRIWVCR R+ GIAT+L++VAR+ +YG ++ ++++AWSQ
Sbjct: 194 VYDSRRLVQNVYPDFKIGISRIWVCRTARKLGIATKLIDVARENIVYGEVIPRYQVAWSQ 253
Query: 244 PSQSGSILAKSYNAAKHRSGKLLIPCYI 271
P+ SG LA YN H+SGKLL+P YI
Sbjct: 254 PTDSGGKLASKYNGIMHKSGKLLLPVYI 281
>SPBC16A3.11_2 [L] KOG3014 Protein involved in establishing cohesion between
sister chromatids during DNA replication
Length = 238
Score = 83.6 bits (205), Expect = 3e-16
Identities = 68/247 (27%), Positives = 109/247 (43%), Gaps = 53/247 (21%)
Query: 26 VKCPKCSITYSTNSPSDLVQHKRYHDLHLNGKRWSQSWGDIINRVEALKTEKQLKYSMVS 85
V C +CS+ Y++ S D++ H R+H L G S I
Sbjct: 19 VTCSECSMEYNSTSEEDILLHSRFHSRVLGGVTVSFQCSPIYR----------------- 61
Query: 86 EKDKKVMSFQLPSQTLYEEDEYIVMISPKKANEVKAALDLMSIVNEELNAPHDENAFWSE 145
+++ L S +Y + +I +KA E +S VN EL++
Sbjct: 62 ------VNYGLSSDCIYSINSESSLIDQRKAEEA------LSFVNNELSS--------EP 101
Query: 146 VDKSGKSQGKAFIYVKKNRAVGVITIEYIE----------NTNRGKWMVLDTKAIVPNVV 195
++ G + F+++ + VG++ E I N N + K N+
Sbjct: 102 IETIGVDKYTTFLFISDKKCVGLLLAERISSAYIVDELELNNNNSTSSAVYIKN--ENLR 159
Query: 196 PDVKLGISRIWVCRNQRQHGIATRLLEVARKKSIYGCIVNKWELAWSQPSQSGSILAKSY 255
LGISRIWV ++R+ GIA+ LL+ A KK IYG +++ E+A+SQPS+SG K +
Sbjct: 160 KGFVLGISRIWVSASRRKQGIASLLLDNALKKFIYGYVISPAEVAFSQPSESG----KQF 215
Query: 256 NAAKHRS 262
+ HRS
Sbjct: 216 IISWHRS 222
>Hs18570262 [L] KOG3014 Protein involved in establishing cohesion between
sister chromatids during DNA replication
Length = 249
Score = 62.4 bits (150), Expect = 7e-10
Identities = 60/240 (25%), Positives = 105/240 (43%), Gaps = 52/240 (21%)
Query: 28 CPKCSITYSTNSPSDLVQHKRYHDLHLNGKRWSQSWGDIINRVEALKTEKQLKYSMVSEK 87
C C + Y+ ++P D +QH ++H L G ++ W +K
Sbjct: 37 CKSCGMIYTASNPEDEMQHVQHHHRFLEGIKYV-GW----------------------KK 73
Query: 88 DKKVMSFQLPSQTLYEEDEYIVMISPKKAN-EVKAALDLMSIVNEELNAPHDENAFWSEV 146
++ V F D IV++ P + +K D+ +V+ EL + +V
Sbjct: 74 ERVVAEFW---------DGKIVLVLPHDPSFAIKKVEDVQELVDNELG--------FQQV 116
Query: 147 DKSGKSQGKAFIYVK-KNRAVGVITIEYIENTNR------GKWMVLDT---KAIVPNVVP 196
++ K F+++ + R VG + E I+ R G T +A + VP
Sbjct: 117 VPKCPNKIKTFLFISDEKRVVGCLIAEPIKQAFRVLSEPIGPESPSSTECPRAWQCSDVP 176
Query: 197 DVKL-GISRIWVCRNQRQHGIATRLLEVARKKSIYGCIVNKWELAWSQPSQSGSILAKSY 255
+ + GISRIWV R +R+ IA RL++ R ++GC ++ E+A+S P+ G + A Y
Sbjct: 177 EPAVCGISRIWVFRLKRRKRIARRLVDTLRNCFMFGCFLSTDEIAFSDPTPDGKLFATKY 236
>Hs16163800_2 [L] KOG3014 Protein involved in establishing cohesion between
sister chromatids during DNA replication
Length = 343
Score = 58.5 bits (140), Expect = 1e-08
Identities = 55/245 (22%), Positives = 105/245 (42%), Gaps = 54/245 (22%)
Query: 26 VKCPKCSITYSTNSPSDLVQHKRYHDLHLNGKRWSQSWGDIINRVEALKTEKQLKYSMVS 85
V C C + Y+ ++P D QH +H+ ++ ++ V
Sbjct: 120 VSCNVCGMLYTASNPEDETQHLLFHNQFISAVKY------------------------VG 155
Query: 86 EKDKKVMSFQLPSQTLYEEDEYIVMISPKKANEVKAALDLMSIVNEELNAPHDENAFWSE 145
K +++++ + P D I+M+ P+ + K AL + + E + D + + +
Sbjct: 156 WKKERILA-EYP-------DGRIIMVLPE---DPKYALKKVDEIREMV----DNDLGFQQ 200
Query: 146 VDKSGKSQGKAFIYVKKNR-AVGVITIEYIE-------------NTNRGKWMVLDTKAIV 191
S+ K +++ ++ VG + E+I+ + K KA
Sbjct: 201 APLMCYSRTKTLLFISNDKKVVGCLIAEHIQWGYRVIEEKLPVIRSEEEKVRFERQKAWC 260
Query: 192 PNVVPDVKL-GISRIWVCRNQRQHGIATRLLEVARKKSIYGCIVNKWELAWSQPSQSGSI 250
+ +P+ + GISRIWV R+ IA+R++E R IYG ++K E+A+S P+ G +
Sbjct: 261 CSTLPEPAICGISRIWVFSMMRRKKIASRMIECLRSNFIYGSYLSKEEIAFSDPTPDGKL 320
Query: 251 LAKSY 255
A Y
Sbjct: 321 FATQY 325
>7295257_2 [L] KOG3014 Protein involved in establishing cohesion between
sister chromatids during DNA replication
Length = 241
Score = 53.5 bits (127), Expect = 3e-07
Identities = 51/233 (21%), Positives = 97/233 (40%), Gaps = 48/233 (20%)
Query: 27 KCPKCSITYSTNSPSDLVQHKRYHDLHLNGKRWSQSWGDIINRVEALKTEKQLKYSMVSE 86
+C +C + Y+ + P + + H+ YH N + L+ + + +VS
Sbjct: 42 QCQQCGLVYTVHEPEEELLHREYH-----------------NSIHVLRFKGWIDEDIVSV 84
Query: 87 KDKKVMSFQLPSQTLYEEDEYIVMISPKKAN-EVKAALDLMSIVNEELNAPHDENAFWSE 145
+ + D I+ I+ + + DL+ +V++EL
Sbjct: 85 YPE------------WASDGRIIRINERAPTARLDRLRDLIGVVDKELGY---------- 122
Query: 146 VDKSGKSQGK---AFIYVKKNRAVGVITIEYIENTNRGKWMVLDTKAIVPNVVPDVKLGI 202
S K AFI V+K + VG ++ + +R ++ +D G+
Sbjct: 123 ---SSYIVPKIFVAFIAVRKQQIVGFCLVQPLSQAHR--FIQVDGTDYFSEESYPASCGV 177
Query: 203 SRIWVCRNQRQHGIATRLLEVARKKSIYGCIVNKWELAWSQPSQSGSILAKSY 255
SRIWV QR+ GIA++LL V + ++ G + + +A+S P+ G LA+ +
Sbjct: 178 SRIWVSPLQRRSGIASKLLRVVQCHTVLGQEIARECIAFSTPTDDGRALARQF 230
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.130 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,765,803
Number of Sequences: 60738
Number of extensions: 694984
Number of successful extensions: 2112
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2101
Number of HSP's gapped (non-prelim): 9
length of query: 271
length of database: 30,389,216
effective HSP length: 105
effective length of query: 166
effective length of database: 24,011,726
effective search space: 3985946516
effective search space used: 3985946516
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)