ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1655 good E KOG2770 Amino acid transport and metabolism Aminomethyl transferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1655 592003 593181 393
(393 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR019c [E] KOG2770 Aminomethyl transferase 494 e-140
SPAC31G5.14 [E] KOG2770 Aminomethyl transferase 325 7e-89
At1g11860 [E] KOG2770 Aminomethyl transferase 298 1e-80
Hs4502083 [E] KOG2770 Aminomethyl transferase 270 4e-72
CE09620 [E] KOG2770 Aminomethyl transferase 264 2e-70
7297745 [E] KOG2770 Aminomethyl transferase 258 1e-68
Hs7019365 [E] KOG2844 Dimethylglycine dehydrogenase precursor 86 1e-16
>YDR019c [E] KOG2770 Aminomethyl transferase
Length = 400
Score = 494 bits (1272), Expect = e-140
Identities = 262/397 (65%), Positives = 302/397 (75%), Gaps = 15/397 (3%)
Query: 4 FRRFNSSSPGSAALKKTALYDLHVSLGGTMVPFAGYSMPVLYKGATHIESHQWTREHAGL 63
F+RFNS+ LKKTAL+DLHVSLGGTMVP+AGYSMPVLYKG THIESH WTR +AGL
Sbjct: 9 FKRFNST------LKKTALHDLHVSLGGTMVPYAGYSMPVLYKGQTHIESHNWTRTNAGL 62
Query: 64 FDVSHMLQSTLKGPKSIEFLHKVTPTDFKALEPKNGTLSVLLNENGGIVDDTLITKINDE 123
FDVSHMLQS L GP S++FL +VTPTDF AL +GTLSVLLN GG+VDDT+ITK ND+
Sbjct: 63 FDVSHMLQSKLSGPHSVKFLQRVTPTDFNALPVGSGTLSVLLNPQGGVVDDTIITKENDD 122
Query: 124 -EFYIVTNAGCIERDTEFLKDELSKISE-GVSWETLKDRSLLALQGPQARYA----LAKL 177
EFYIVTNAGC ERDTEF DEL S W+ ++ RSLLALQGP+A+ L+K
Sbjct: 123 NEFYIVTNAGCAERDTEFFHDELQNGSTLDCQWKIIEGRSLLALQGPKAKDVLEPLLSKT 182
Query: 178 VKHGDLKTLYFGQRDDFELTENISAQVARSGYTGEDGFEISVLNKXXXXXXXXXXXXPEV 237
DLK L+FGQR +F L + Q+AR GYTGEDGFEIS+ N+ P +
Sbjct: 183 APGKDLKELFFGQRHEFALKDGSLVQIARGGYTGEDGFEISIANEKAVEFAEQLLANPVM 242
Query: 238 KPIGLAARDSLRLEAGMCLYGHELSESITPVEAALNWVISKSRRDGSLGK--FNGFDNII 295
KPIGLAARDSLRLEAGMCLYGHEL ESITPVEAALNWVISKSRRD K FNG+ I+
Sbjct: 243 KPIGLAARDSLRLEAGMCLYGHELDESITPVEAALNWVISKSRRDLVDQKYWFNGYAKIM 302
Query: 296 AQIKDKSYDKVRVGFKYQGKGPAARQDTPI-LNDSDEQVGVVTSGSASPSLDGINIGQAY 354
Q+ +K+Y KVRVGFKY KGPAAR I L D++ +VG+VTSGSASP+L+ INIGQAY
Sbjct: 303 DQLNNKTYSKVRVGFKYLKKGPAARNGVKIFLPDAETEVGLVTSGSASPTLNNINIGQAY 362
Query: 355 VKKGLHKKGTQLKVQVRKKTYPIEIVKMPLVPTHYYR 391
V+KG HKKGT+L VQVR K YPIE+ KMPLVPTHYY+
Sbjct: 363 VQKGYHKKGTKLLVQVRNKFYPIELAKMPLVPTHYYK 399
>SPAC31G5.14 [E] KOG2770 Aminomethyl transferase
Length = 387
Score = 325 bits (833), Expect = 7e-89
Identities = 176/390 (45%), Positives = 247/390 (63%), Gaps = 9/390 (2%)
Query: 2 LQFRRFNSSSPGSAALKKTALYDLHVSLGGTMVPFAGYSMPVLYKGATHIESHQWTREHA 61
L + SS+ S++LK+T LYDLH+ G T+VPFAG+SMPV YKG T SH+WTREH+
Sbjct: 7 LSILKRQSSTAASSSLKRTPLYDLHLKEGATIVPFAGFSMPVQYKGQTISASHKWTREHS 66
Query: 62 GLFDVSHMLQSTLKGPKSIEFLHKVTPTDFKALEPKNGTLSVLLNENGGIVDDTLITKIN 121
GLFDVSHM+Q ++G + +L +TP+ K L+P + TLS NE GGI+DDT+I+K +
Sbjct: 67 GLFDVSHMVQWFVRGENATAYLESITPSSLKELKPFHSTLSAFTNETGGIIDDTIISKQD 126
Query: 122 DEEFYIVTNAGCIERDTEFLKDELSKISEGVSWETLKDRSLLALQGPQARYALAKLVKHG 181
+ +YIVTNA C E+D LK + +GV E ++ R+L+A+QGP+ + KL+ +
Sbjct: 127 ENTYYIVTNAACSEKDEANLKKHIEN-WKGVELERVQGRALIAIQGPETASVVQKLIPNV 185
Query: 182 DLKTLYFGQRDDFELTENISAQVARSGYTGEDGFEISVLNKXXXXXXXXXXXXPEVKPIG 241
D L FGQ + + + +RSGYTGEDGFE+S+ + V+PIG
Sbjct: 186 DFSVLKFGQSAYVDF-KGVKCLFSRSGYTGEDGFEVSIPEEVSVDFASTLLADTRVRPIG 244
Query: 242 LAARDSLRLEAGMCLYGHELSESITPVEAALNWVISKSRRDGSLGKFNGFDNIIAQIKDK 301
L ARD+LRLEAGMCLYG ++ ++ +PVE +L+W+I K RR G F G I+ ++KD
Sbjct: 245 LGARDTLRLEAGMCLYGSDIDDTTSPVEGSLSWIIGKRRR--KEGGFVGSSRILKELKD- 301
Query: 302 SYDKVRVGFKYQGKGPAARQDTPILNDSDEQVGVVTSGSASPSLDGINIGQAYVKKGLHK 361
+ RVGF + K P AR + + D E VG VTSG SP+L G NI Y+ GLH+
Sbjct: 302 GPSRRRVGFIVE-KVP-ARHGSAVEVDGVE-VGQVTSGCPSPTL-GKNIAMGYISTGLHQ 357
Query: 362 KGTQLKVQVRKKTYPIEIVKMPLVPTHYYR 391
GT ++VR K +P ++V+MP V THYY+
Sbjct: 358 VGTPAHIKVRNKLHPAQVVRMPFVETHYYK 387
>At1g11860 [E] KOG2770 Aminomethyl transferase
Length = 408
Score = 298 bits (762), Expect = 1e-80
Identities = 179/393 (45%), Positives = 238/393 (60%), Gaps = 17/393 (4%)
Query: 5 RRFNSSSPGSAALKKTALYDLHVSLGGTMVPFAGYSMPVLYKGATHIESHQWTREHAGLF 64
RR+ +S A LKKTALYD HV+ GG MVPFAG+SMP+ YK + ++S RE+ LF
Sbjct: 27 RRYFASE---ADLKKTALYDFHVAHGGKMVPFAGWSMPIQYKDSI-MDSTVNCRENGSLF 82
Query: 65 DVSHMLQSTLKGPKSIEFLHKVTPTDFKALEPKNGTLSVLLNENGGIVDDTLITKINDEE 124
DV+HM +LKG + FL + D L P G+L+V NE GG +DD++ITK+ DE
Sbjct: 83 DVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITKVTDEH 142
Query: 125 FYIVTNAGCIERDTEFLKDELSKI-SEG--VSWETLKDRSLLALQGPQARYALAKLVKHG 181
Y+V NAGC ++D +++ + S+G VSW +RSLLALQGP A L L K
Sbjct: 143 IYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDERSLLALQGPLAAPVLQHLTKE- 201
Query: 182 DLKTLYFGQRDDFELTENISAQVARSGYTGEDGFEISVLNKXXXXXXXXXXXXPE--VKP 239
DL LYFG ++ + + + R+GYTGEDGFEISV ++ E V+
Sbjct: 202 DLSKLYFGNFQILDINGS-TCFLTRTGYTGEDGFEISVPDEHAVDLAKAILEKSEGKVRL 260
Query: 240 IGLAARDSLRLEAGMCLYGHELSESITPVEAALNWVISKSRRDGSLGKFNGFDNIIAQIK 299
GL ARDSLRLEAG+CLYG+++ + I+PVEA L W I K RR + G F G D I+ Q+K
Sbjct: 261 TGLGARDSLRLEAGLCLYGNDMEQHISPVEAGLTWAIGKRRR--AEGGFLGADVILQQLK 318
Query: 300 DKSYDKVRVGFKYQGKGPAARQDTPILNDSDEQVGVVTSGSASPSLDGINIGQAYVKKGL 359
D + RVGF GP AR + + ++S ++G +TSG SP+L NI YVK G
Sbjct: 319 DGPTIR-RVGF--FSSGPPARSHSEVHDESGNKIGEITSGGFSPNLKK-NIAMGYVKSGQ 374
Query: 360 HKKGTQLKVQVRKKTYPIEIVKMPLVPTHYYRP 392
HK GT++K+ VR K Y I KMP V T YY+P
Sbjct: 375 HKTGTKVKILVRGKPYEGSITKMPFVATKYYKP 407
>Hs4502083 [E] KOG2770 Aminomethyl transferase
Length = 403
Score = 270 bits (689), Expect = 4e-72
Identities = 163/377 (43%), Positives = 215/377 (56%), Gaps = 12/377 (3%)
Query: 17 LKKTALYDLHVSLGGTMVPFAGYSMPVLYKGATHIESHQWTREHAGLFDVSHMLQSTLKG 76
L++T LYD H++ GG MV FAG+S+PV Y+ + H +SH TR+H LFDVSHMLQ+ + G
Sbjct: 33 LRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDS-HTDSHLHTRQHCSLFDVSHMLQTKILG 91
Query: 77 PKSIEFLHKVTPTDFKALEPKNGTLSVLLNENGGIVDDTLITKINDEEFYIVTNAGCIER 136
+ + + D L P GTLS+ NE GGI+DD ++T ++ Y+V+NAGC E+
Sbjct: 92 SDRCKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEK 151
Query: 137 DTEFLKD---ELSKISEGVSWETLKDRSLLALQGPQARYALAKLVKHGDLKTLYFGQRDD 193
D ++D EL V E L D +LLALQGP A L V DL+ L F
Sbjct: 152 DLALMQDKVRELQNQGRDVGLEVL-DNALLALQGPTAAQVLQAGVA-DDLRKLPFMTSAV 209
Query: 194 FELTENISAQVARSGYTGEDGFEISVLNKXXXXXXXXXXXXPEVKPIGLAARDSLRLEAG 253
E+ +V R GYTGEDG EISV PEVK GLAARDSLRLEAG
Sbjct: 210 MEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAG 269
Query: 254 MCLYGHELSESITPVEAALNWVISKSRRDGSLGKFNGFDNIIAQIKDKSYDKVRVGFKYQ 313
+CLYG+++ E TPVE +L+W + K RR + F G I+ Q+K + + RVG +
Sbjct: 270 LCLYGNDIDEHTTPVEGSLSWTLGKRRR--AAMDFPGAKVIVPQLKGR-VQRRRVGLMCE 326
Query: 314 GKGPAARQDTPILNDSDEQVGVVTSGSASPSLDGINIGQAYVKKGLHKKGTQLKVQVRKK 373
G R +PILN ++G VTSG SPSL N+ YV + GT L V+VR+K
Sbjct: 327 --GAPMRAHSPILNMEGTKIGTVTSGCPSPSLKK-NVAMGYVPCEYSRPGTMLLVEVRRK 383
Query: 374 TYPIEIVKMPLVPTHYY 390
+ KMP VPT+YY
Sbjct: 384 QQMAVVSKMPFVPTNYY 400
>CE09620 [E] KOG2770 Aminomethyl transferase
Length = 402
Score = 264 bits (674), Expect = 2e-70
Identities = 161/390 (41%), Positives = 225/390 (57%), Gaps = 17/390 (4%)
Query: 10 SSPGSAALKKTALYDLHVSLGGTMVPFAGYSMPVLYKGATHIESHQWTREHAGLFDVSHM 69
S A+ K+T L + H GG +V FAGY MP Y + ES TR+H LFDVSHM
Sbjct: 18 SRTAEASAKQTCLIETHKKHGGKLVEFAGYDMPTQYADFSIKESTIHTRKHVSLFDVSHM 77
Query: 70 LQSTLKGPKSIEFLHKVTPTDFKALEPKNGTLSVLLNENGGIVDDTLITKINDEEFYIVT 129
LQ+ + G + F+ +T D + L+ +GTLSV NE GGI DD +I K + + ++VT
Sbjct: 78 LQTYITGKDRVAFIESLTTADVQGLQENSGTLSVFTNEKGGIKDDLIIMKTDKDFLFLVT 137
Query: 130 NAGCIERDTEFLKDELS---KISEGVSWETLKDRSLLALQGPQARYALAKLVKHG---DL 183
NAGCIE+D +L++ + + V ETL +R L+A+QGP+ +AK+++ G DL
Sbjct: 138 NAGCIEKDLPYLQENAAAWRSKGKDVKIETLDNRGLVAVQGPE----MAKVLQEGTDIDL 193
Query: 184 KTLYFGQRDDFELTENISAQVARSGYTGEDGFEISV--LNKXXXXXXXXXXXXPEVKPIG 241
L F + ++ +V R GYTGEDG EISV VK G
Sbjct: 194 SKLTFMKTTVGKVFGIDGCRVTRCGYTGEDGVEISVDPTKAEQLVERLLASQAGSVKLAG 253
Query: 242 LAARDSLRLEAGMCLYGHELSESITPVEAALNWVISKSRRDGSLGKFNGFDNIIAQIKDK 301
L ARD+LRLEAG+CLYG ++ E+ TP+EA L +V++K RR+ F G ++I+ Q+K+K
Sbjct: 254 LGARDALRLEAGLCLYGSDIEENTTPIEAGLAFVVAKRRRETL--DFPGAEHIVKQLKEK 311
Query: 302 SYDKVRVGFKYQGKGPAARQDTPILNDSDE-QVGVVTSGSASPSLDGINIGQAYVKKGLH 360
S+ K RVG G R P+++ D+ +G VTSG SP+L G NI AYV K
Sbjct: 312 SWPKRRVGL-LAPAGRCPRSHLPLIDPLDKCSIGFVTSGCPSPTL-GKNIAIAYVDKSHS 369
Query: 361 KKGTQLKVQVRKKTYPIEIVKMPLVPTHYY 390
K GT+ V K P+E+VKMP VPT+Y+
Sbjct: 370 KIGTKFVVDFGAKQAPVEVVKMPFVPTNYF 399
>7297745 [E] KOG2770 Aminomethyl transferase
Length = 405
Score = 258 bits (659), Expect = 1e-68
Identities = 164/396 (41%), Positives = 222/396 (55%), Gaps = 15/396 (3%)
Query: 6 RFNSSSPGSAALKKTALYDLHVSLGGTMVPFAGYSMPVLYKGATHIESHQWTREHAGLFD 65
R SS+ G ++TALYD HV GG +V F GY++PV Y + I SH TR+ +FD
Sbjct: 16 RHASSAAGEG--QRTALYDFHVQKGGKIVNFGGYALPVQYSDQSIIASHLHTRQVGSIFD 73
Query: 66 VSHMLQSTLKGPKSIEFLHKVTPTDFKALEPKNGTLSVLLNENGGIVDDTLITKINDEEF 125
VSHMLQS + G + L V D +G+L+V NE GGI+DD ++ K++++E
Sbjct: 74 VSHMLQSRIFGKDAAACLESVCTADILGTPEGSGSLTVFTNEAGGILDDLIVNKVSEKEL 133
Query: 126 YIVTNAGCIERDTEFLK---DELSKISEGVSWE--TLKDRSLLALQGPQARYALAKLVK- 179
Y+V+NA E+D +K D + VS E T D+SL+A+QGPQ L+KL+
Sbjct: 134 YVVSNAAMKEQDMGIMKTAVDNFKSQGKDVSIEFLTPADQSLVAVQGPQVAKELSKLLTG 193
Query: 180 HGDLKTLYFGQRDDFELTENISAQVARSGYTGEDGFEISVLNKXXXXXXXXXXXXPEVKP 239
L LYF L + ++ R GYTGEDG EISV + +K
Sbjct: 194 KASLDQLYFMSSFVTTLAGIPNVRITRCGYTGEDGVEISVASSQAQKLTESLLESGVLKL 253
Query: 240 IGLAARDSLRLEAGMCLYGHELSESITPVEAALNWVISKSRRDGSLGKFNGFDNIIAQIK 299
GL ARDSLRLEAG+CLYG ++ TPVEAAL W+++K RR + F G D I+ Q+K
Sbjct: 254 AGLGARDSLRLEAGLCLYGSDIDSKTTPVEAALAWLVTKRRR--TTRDFPGADVILGQLK 311
Query: 300 DKSYDKVRVGFKYQG-KGPAARQDTPILNDSDEQVGVVTSGSASPSLDGINIGQAYVKKG 358
+ + RVG + G K P AR I + +QVG VTSG SPS G NI YV +
Sbjct: 312 E-GVSRRRVGLQMLGTKPPPARSGVAIFSQG-QQVGQVTSGCPSPSA-GRNIAMGYVAEN 368
Query: 359 LHKKGTQLKVQVRKKTYPIEIVKMPLVPTHYY-RPE 393
L GT+++ +VR K Y E+ KMP V +YY RP+
Sbjct: 369 LKAPGTKVEFKVRDKLYEAEVTKMPFVKANYYNRPK 404
>Hs7019365 [E] KOG2844 Dimethylglycine dehydrogenase precursor
Length = 866
Score = 85.9 bits (211), Expect = 1e-16
Identities = 89/339 (26%), Positives = 147/339 (43%), Gaps = 25/339 (7%)
Query: 59 EHAGLFDVSHMLQSTLKGPKSIEFLHKVTPTDFKALEPKNG--TLSVLLNENGGIVDDTL 116
+ G+ D+S + +KG SI L + F + PK G +S +L G + +
Sbjct: 527 QRVGVTDLSPFGKFNIKGQDSIRLLDHL----FANVIPKVGFTNISHMLTPKGRVYAELT 582
Query: 117 ITKINDEEFYIVTNAGCIERDTEFLKDELSKISEGVSWETLKDR-SLLALQGPQARYALA 175
++ + EF ++T +G D ++++E K V + + D +L + GPQAR L
Sbjct: 583 VSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQ 642
Query: 176 KLVKHGDLKTLYFGQRDDFELTENISAQVARSGYTGEDGFEISVLNKXXXXXXXXXXXXP 235
KL ++ + NI R YTGE G+E+ +
Sbjct: 643 KLTPEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAG 702
Query: 236 EVKPI---GLAARDSLRLEAGMCLYGHELSESITPVEAALNWVISKSRRDGSLGKFNGFD 292
+ + I G A ++LRLE +G E++ P+EA L + + ++ +GK
Sbjct: 703 QEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKPADFIGK----- 757
Query: 293 NIIAQIKDKSYDKVRVGFKYQGKGPAARQDTPILNDS----DEQVGVVTSGSASPSLDGI 348
+ QIK K + V A P N+S + VG TSGS S S+
Sbjct: 758 QALKQIKAKGLKRRLVCLTL-----ATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQK- 811
Query: 349 NIGQAYVKKGLHKKGTQLKVQVRKKTYPIEIVKMPLVPT 387
++ AYV L + G Q++V++ K YP I++ PLV T
Sbjct: 812 SLAFAYVPVQLSEVGQQVEVELLGKNYPAVIIQEPLVLT 850
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,098,390
Number of Sequences: 60738
Number of extensions: 964434
Number of successful extensions: 2158
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2119
Number of HSP's gapped (non-prelim): 7
length of query: 393
length of database: 30,389,216
effective HSP length: 108
effective length of query: 285
effective length of database: 23,829,512
effective search space: 6791410920
effective search space used: 6791410920
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)