ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1721 good H KOG2586 Coenzyme transport and metabolism Pyridoxamine-phosphate oxidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1721 614589 615263 225
(225 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR035c [H] KOG2586 Pyridoxamine-phosphate oxidase 336 1e-92
SPAC1093.02 [H] KOG2586 Pyridoxamine-phosphate oxidase 242 3e-64
At5g49970_2 [H] KOG2586 Pyridoxamine-phosphate oxidase 148 5e-36
7298947 [H] KOG2586 Pyridoxamine-phosphate oxidase 139 3e-33
Hs8922498 [H] KOG2586 Pyridoxamine-phosphate oxidase 137 1e-32
CE01335 [H] KOG2586 Pyridoxamine-phosphate oxidase 125 4e-29
>YBR035c [H] KOG2586 Pyridoxamine-phosphate oxidase
Length = 228
Score = 336 bits (862), Expect = 1e-92
Identities = 155/224 (69%), Positives = 183/224 (81%)
Query: 2 ADDTKTPIIFAPETYQYDKFSLDESTIEKDPIGQFTKWFNQAKEDPTEMIPESVAFATAE 61
A++T+ PIIFAPETYQYDKF+L+E + DPI FTKWFN+AKEDP E +PE++ F++AE
Sbjct: 5 AEETQKPIIFAPETYQYDKFTLNEKQLTDDPIDLFTKWFNEAKEDPRETLPEAITFSSAE 64
Query: 62 LPSGKVSCRILLFKELDDRGFTIYSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGF 121
LPSG+VS RILLFKELD RGFTIYSNW TSRKA DI +NP AA+ FFWK+LQRQVRVEG
Sbjct: 65 LPSGRVSSRILLFKELDHRGFTIYSNWGTSRKAHDIATNPNAAIVFFWKDLQRQVRVEGI 124
Query: 122 TEHVSRETSERYFKTRPRNSKIGAWSSPQSQVIDNRXXXXXXXXXXXXKFKDADDVECPQ 181
TEHV+RETSERYFKTRPR SKIGAW+S QS VI NR +FKDA+D+ CP
Sbjct: 125 TEHVNRETSERYFKTRPRGSKIGAWASRQSDVIKNREELDELTQKNTERFKDAEDIPCPD 184
Query: 182 FWGGIRVVPLRIEFWQGRASRLHDRLVFERKSENDEWELVRLAP 225
+WGG+R+VPL IEFWQGR SRLHDR V+ RK+END W++VRLAP
Sbjct: 185 YWGGLRIVPLEIEFWQGRPSRLHDRFVYRRKTENDPWKVVRLAP 228
>SPAC1093.02 [H] KOG2586 Pyridoxamine-phosphate oxidase
Length = 231
Score = 242 bits (618), Expect = 3e-64
Identities = 122/226 (53%), Positives = 157/226 (68%), Gaps = 3/226 (1%)
Query: 3 DDTKTPIIFAPETYQYDKFSLD-ESTIEKDPIGQFTKWFNQAKEDPTEMIPESVAFATAE 61
D T +IFAP YQY+K SL ++ + KDP+ F +WF +A +D PES +TA
Sbjct: 6 DSTHEKLIFAPSRYQYEKSSLHRDALMGKDPLVLFNQWFQEATDDEGIKSPESTTLSTAR 65
Query: 62 LPSGKVSCRILLFKELDDRGFTIYSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGF 121
LPSG+VS R++L KELD RGF I++N TS+KA D+KSNP A+L+F+W+ LQRQVRVEG
Sbjct: 66 LPSGRVSSRLVLLKELDHRGFIIFTNLGTSKKAKDLKSNPYASLSFWWEPLQRQVRVEGI 125
Query: 122 TEHVSRETSERYFKTRPRNSKIGAWSSPQSQVIDNRXXXXXXXXXXXXKFKDADD--VEC 179
E +SRE +E YFKTRPRNS+IGAW+SPQS+VI +R KF + + V
Sbjct: 126 IERLSREETEEYFKTRPRNSRIGAWASPQSEVIADREELEKRVEEYKKKFGEDESVPVPV 185
Query: 180 PQFWGGIRVVPLRIEFWQGRASRLHDRLVFERKSENDEWELVRLAP 225
P FWGGIR+VPL IEFWQG RLHDR F R + ++++ELVRLAP
Sbjct: 186 PDFWGGIRIVPLEIEFWQGGKYRLHDRFSFRRNTLDEDYELVRLAP 231
>At5g49970_2 [H] KOG2586 Pyridoxamine-phosphate oxidase
Length = 221
Score = 148 bits (374), Expect = 5e-36
Identities = 82/203 (40%), Positives = 114/203 (55%), Gaps = 5/203 (2%)
Query: 25 ESTIEKDPIGQFTKWFNQAKEDPTEMIPESVAFATAELPSGKVSCRILLFKELDDRGFTI 84
E +E DP QF KWF++A ++A +TA K S R++L K D+ GF
Sbjct: 22 EEQVETDPTVQFRKWFDEAVAAGLRET-NAMALSTANKDK-KPSSRMVLLKGFDENGFVW 79
Query: 85 YSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGFTEHVSRETSERYFKTRPRNSKIG 144
++N+E S+K D+ NP AAL F+W+ L RQVR+EG E + SE YF +RPR S+IG
Sbjct: 80 FTNYE-SKKGSDLSENPSAALLFYWEILNRQVRIEGPVERIPESESENYFHSRPRGSQIG 138
Query: 145 AWSSPQSQVIDNRXXXXXXXXXXXXKFKDADDVECPQFWGGIRVVPLRIEFWQGRASRLH 204
A S QS V+ R ++ D + P+ WGG R+ P EFWQG+ SRLH
Sbjct: 139 AIVSKQSSVVPGRHVLYDEYEELTKQYSDGSVIPKPKNWGGFRLKPNLFEFWQGQPSRLH 198
Query: 205 DRLVFERK--SENDEWELVRLAP 225
DRL + + + N W++ RLAP
Sbjct: 199 DRLQYSLQDVNGNPAWKIHRLAP 221
>7298947 [H] KOG2586 Pyridoxamine-phosphate oxidase
Length = 484
Score = 139 bits (350), Expect = 3e-33
Identities = 80/189 (42%), Positives = 107/189 (56%), Gaps = 3/189 (1%)
Query: 23 LDESTIEKDPIGQFTKWFNQAKEDPTEMIPESVAFATAELPSGKVSCRILLFKELDDRGF 82
L+++ K+P F W A + P + P + A AT G+ S R +L KE GF
Sbjct: 25 LEDNIKVKNPFCVFRDWLELALKTPEILEPNAAALATVSA-EGRPSNRYVLVKEATAEGF 83
Query: 83 TIYSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGFTEHVSRETSERYFKTRPRNSK 142
T ++N+ SRKA+DIKSNP A++F+W L+R VR+EG E +S E S +YF RPR S+
Sbjct: 84 TFFTNYG-SRKAEDIKSNPYVAISFYWLPLRRSVRIEGVAEKISVEDSLKYFHQRPRASQ 142
Query: 143 IGAWSSPQSQVIDNRXXXXXXXXXXXXKFKDADDVECPQFWGGIRVVPLRIEFWQGRASR 202
IGA +SPQSQ I +R + +V P WGG V P IEFWQG+ R
Sbjct: 143 IGAAASPQSQRIPSRSYLDDVEAAIKLELGPDGEVPLPN-WGGYLVRPDLIEFWQGQTDR 201
Query: 203 LHDRLVFER 211
LHDR+ F R
Sbjct: 202 LHDRIRFRR 210
Score = 64.3 bits (155), Expect = 1e-10
Identities = 43/184 (23%), Positives = 80/184 (43%), Gaps = 6/184 (3%)
Query: 16 YQYDKFSLDESTIEKDPIGQFTKWFNQAKEDPTEMIPESVAFATAELPSGKVSCRILLFK 75
+Q +E + ++P F W A+++ ++ P AT + SG+ R+ +
Sbjct: 253 HQVSCIGSEEQRVYEEPHVIFQNWLMAAQKEAPQVRPRLACMATVD-KSGEPVTRLTSIE 311
Query: 76 ELDDRGFTIYSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGFTEHVSRETSERYFK 135
E++ G T ++ SR+A +I +NP +L F W L R VR+ G ++ E F+
Sbjct: 312 EVNSHGITFFTTLG-SRQAGEISANPHVSLHFNWAPLMRSVRIAGSAHQLTEEQVRDQFR 370
Query: 136 TRPRNSKIGAWSSPQSQVID--NRXXXXXXXXXXXXKF--KDADDVECPQFWGGIRVVPL 191
PR+ ++ P+ + +R + K +++ P WGG + P
Sbjct: 371 RFPRHVQMSITHGPRYAAAEWQSRSGFFARIGQRLNTWLGKQPEEIPMPHNWGGYILTPS 430
Query: 192 RIEF 195
EF
Sbjct: 431 LYEF 434
>Hs8922498 [H] KOG2586 Pyridoxamine-phosphate oxidase
Length = 261
Score = 137 bits (345), Expect = 1e-32
Identities = 83/226 (36%), Positives = 124/226 (54%), Gaps = 17/226 (7%)
Query: 14 ETYQYDKFSLDESTIEK-DPIGQFTKWFNQAKEDPTEMIPESVAFATAELPSGKVSCRIL 72
++Y+ D+ + +E+ + DP+ QF WF +A + P ++ AT GK S R+L
Sbjct: 39 KSYRGDREAFEETHLTSLDPVKQFAAWFEEAVQCPDIGEANAMCLATCTR-DGKPSARML 97
Query: 73 LFKELDDRGFTIYSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGFTEHVSRETSER 132
L K GF ++N+E SRK ++ SNP A+L F+W+ L RQVRVEG + + E +E
Sbjct: 98 LLKGFGKDGFRFFTNFE-SRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAEC 156
Query: 133 YFKTRPRNSKIGAWSSPQSQVIDNRXXXXXXXXXXXXKFKDADDVECPQFWGGIRVVPLR 192
YF +RP++S+IGA S QS VI +R ++D +V P+ WGG + P
Sbjct: 157 YFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQD-QEVPKPKSWGGYVLYPQV 215
Query: 193 IEFWQGRASRLHDRLVFER-------------KSENDEWELVRLAP 225
+EFWQG+ +RLHDR+VF R ++W RLAP
Sbjct: 216 MEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP 261
>CE01335 [H] KOG2586 Pyridoxamine-phosphate oxidase
Length = 253
Score = 125 bits (315), Expect = 4e-29
Identities = 72/211 (34%), Positives = 110/211 (52%), Gaps = 6/211 (2%)
Query: 18 YDKFSLDESTIEKDPIGQFTKWFNQAKEDPTEMIPESVAFATAELPSG-KVSCRILLFKE 76
+D + L+ P F WF E A + + + + S R++L K
Sbjct: 46 HDPYLLESKLPTTSPFELFDIWFRNVASQSDLTFEEINAVSLSTVGKDLRPSSRMVLLKA 105
Query: 77 LDDRGFTIYSNWETSRKADDIKSNPQAALTFFWKNLQRQVRVEGFTEHVSRETSERYFKT 136
GF+ Y+N+ TSRK + ++ NP AA+ F+W + RQ+RVEG E + E + Y+ +
Sbjct: 106 YTPTGFSFYTNY-TSRKGNQLEENPNAAMLFYWPKVNRQIRVEGVVEKLPDEMAVAYWNS 164
Query: 137 RPRNSKIGAWSSPQSQVIDNR--XXXXXXXXXXXXKFKDADDVECPQFWGGIRVVPLRIE 194
RP S+IG+ SS QS+V+ +R + A + P+ WGG ++P E
Sbjct: 165 RPVASRIGSKSSDQSKVVPDREFLESKKVALTELSVREGAQAITKPESWGGYHLIPRYFE 224
Query: 195 FWQGRASRLHDRLVFERKSENDEWELVRLAP 225
FWQG++ RLHDR+VFER + D W L RL+P
Sbjct: 225 FWQGQSDRLHDRIVFER--DVDVWLLKRLSP 253
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,770,396
Number of Sequences: 60738
Number of extensions: 543120
Number of successful extensions: 1128
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1109
Number of HSP's gapped (non-prelim): 7
length of query: 225
length of database: 30,389,216
effective HSP length: 102
effective length of query: 123
effective length of database: 24,193,940
effective search space: 2975854620
effective search space used: 2975854620
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)