ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactII1747   suspect: LH   R     KOG1337  General function prediction only                               N-methyltransferase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactII1747 623052 621394 -553 
         (553 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YBR030w [R] KOG1337 N-methyltransferase                               349   5e-96
At3g56570 [R] KOG1337 N-methyltransferase                             114   3e-25

>YBR030w [R] KOG1337 N-methyltransferase
          Length = 552

 Score =  349 bits (896), Expect = 5e-96
 Identities = 205/568 (36%), Positives = 300/568 (52%), Gaps = 39/568 (6%)

Query: 1   MSNTDSSDTDDILTFVLESKGFFHTAGCEIRRSDYGGLGVFALKDLKEDTVLLRIPKSSV 60
           MS T   D   IL FV    G F  + C+IR S  GGLGVFA  D+ E   +L + KSS+
Sbjct: 1   MSVTFKDDVHRILKFVANCNGRFEDSKCDIRESPLGGLGVFAKTDIAEGESILTLNKSSI 60

Query: 61  FTAENSSIANLLVDSEVKGMLALNIAYIYEVFIFQVESHWYKYLKSISHRQN-GSLYLPP 119
           F+A NSSIANLL DS + GMLALNIA+IYE  +F+  SHWY +L++I  R + G L LPP
Sbjct: 61  FSASNSSIANLLCDSSIDGMLALNIAFIYETTVFRNSSHWYPFLRTIRIRDDEGHLNLPP 120

Query: 120 AYWEDNEKILLKNTALDVVFEALSPQEEIENGFKVGIALSRKWNEEFGLKIPYLFDFNLE 179
           ++W  + K LLK T+ D +F++L+P+EEI  GF++ + L+ KWN+EFGL+IP  F    E
Sbjct: 121 SFWHADAKRLLKGTSFDTLFDSLAPEEEIMEGFEIAVDLAHKWNDEFGLEIPKGFLDVSE 180

Query: 180 DQKQVSSNFK--EFAAIAFAISSRVFEIDNYHDSGLVAIADLFNHHATRPDVRFESMYDV 237
           +  +   N K  +F ++A+ +SSR FEID YH++ LV IADLFNHH + PD++F S+YDV
Sbjct: 181 ENHEEDYNLKLEKFISVAYTLSSRGFEIDAYHETALVPIADLFNHHVSDPDLKFVSLYDV 240

Query: 238 CDMCGELGDCKHVIAETKALDMEMEQRTQRITPRETGTISLSVIEELERELDPXXXXXXX 297
           CD CGE   CKH+IAE       +++   ++   ET  I   +I+ LE +L+        
Sbjct: 241 CDKCGEPDMCKHLIAEEYLEAENLDKNMPKVASMETRVIDEDLIKSLENDLEKEYSNVTA 300

Query: 298 XXXXXXNAEETPEAIMPKNIDTDECVDIILSNDVKAGEEIFNSYGDHSNSYLLARYGFCI 357
                    E P          DECVD++L NDV  G+EIFNSYG+ SN +LLARYGF +
Sbjct: 301 NIEDDDGGIENP----------DECVDLVLKNDVAQGQEIFNSYGELSNVFLLARYGFTV 350

Query: 358 EDNPHDVVDLSEELVEYGQKNSKRIGDRMEWWEDEVHAVYKEWYEATRXXXXXXXXXXXX 417
            +N +D+V L  + ++  +K  K   ++++WW    H ++  WY   R            
Sbjct: 351 PENQYDIVHLGPDFMKILKKEEK-YQEKVKWWSQVGHGLFSAWYAQMRQEDEEDEDGQAK 409

Query: 418 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNDAW-GYVHLKHSGKPNDVLLAILNLLT 476
                                            ++W   +++  SG+P+    A+ NLLT
Sbjct: 410 SDNLSDDIESEEEEEEEEGDDSL----------ESWLSQLYIDSSGEPSPSTWALANLLT 459

Query: 477 MSPSMYKNLSSAR-----GDVXXXXXXXXXXXXXXSGDGKRLLKQICAAKKY-------- 523
           ++   +++L S +      D               +   K+LL  +   K+         
Sbjct: 460 LTAVQWESLFSKKATPHISDSIVNEEKLPFLAKKDNPHSKKLLSNLLKEKQLPCIKGDNS 519

Query: 524 AKLPSDLKTYNLQQIELLINSEKQILNR 551
           +K+ S  K+  LQ    L+ SE  IL+R
Sbjct: 520 SKITSATKSM-LQNARTLVQSEHNILDR 546


>At3g56570 [R] KOG1337 N-methyltransferase
          Length = 531

 Score =  114 bits (286), Expect = 3e-25
 Identities = 97/340 (28%), Positives = 154/340 (44%), Gaps = 35/340 (10%)

Query: 37  GLGVFALKDLKEDTVLLRIPKSSVFTAENSSIANLLVDSEVKGMLALNIAYIYEVFIFQV 96
           G+ V A  DLKE  V+  I K++  T + S    ++  +++ G L L++A +YE  + + 
Sbjct: 33  GVSVRAFCDLKEGDVVANISKTACLTIKTSGAREMIESADLDGSLGLSVALMYERSLGE- 91

Query: 97  ESHWYKYLKSISHRQNGSLYLPPAYWEDNEKILLKNTALDVVFEALSPQEEIENGFKVGI 156
           ES W  YL+ +  +++    LP  +  ++   LL  T L  + +            +  +
Sbjct: 92  ESPWAGYLQILPIQED----LPLVWSLEDLDSLLSGTELHKLVK------------EDHV 135

Query: 157 ALSRKWNEEFGLKIPYLFDFNLEDQKQVSSNFKEFAAIAFAISSRVFEIDNYHDSGLVAI 216
            +   W E   L +      N++     S   KE+ A    I+SR FEID+YH SG+V +
Sbjct: 136 LIYEDWKENI-LPLTSSLPQNVDSD---SFGIKEYLAAKSLIASRSFEIDDYHGSGMVPL 191

Query: 217 ADLFNHHATRPDVRF----ESMYDVCDMCGELGDCKHVIAETKALDMEMEQRTQRITPRE 272
           ADLFNH     DV F    +S  D  D      +       +  +    EQ  + +    
Sbjct: 192 ADLFNHKTGAEDVHFTHESDSEADESDNDDAANETTDEDEPSSKISSSPEQSFEEVPGEN 251

Query: 273 TGTISLSVIEELERELDPXXXXXXXXXXXXXNAEETPEAIMPKNIDTDECVDIILSNDVK 332
           T   +    EE E E +                EE   + M +N  +   + +I+  DV 
Sbjct: 252 TDDEAKEEEEEEEEEEE--------GEEEEEGEEEEENSSMLQNDQSG--LKMIMVKDVS 301

Query: 333 AGEEIFNSYGDHSNSYLLARYGFCIEDNPHDVVDLSEELV 372
           AG E+FN+YG   N+ LL RYGF   DNP+D+V++  ELV
Sbjct: 302 AGAEVFNTYGLMGNAALLHRYGFTELDNPYDIVNIDLELV 341


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.317    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,096,311
Number of Sequences: 60738
Number of extensions: 1150653
Number of successful extensions: 2671
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2663
Number of HSP's gapped (non-prelim): 3
length of query: 553
length of database: 30,389,216
effective HSP length: 111
effective length of query: 442
effective length of database: 23,647,298
effective search space: 10452105716
effective search space used: 10452105716
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)