ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII1994 good I KOG0873 Lipid transport and metabolism C-4 sterol methyl oxidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII1994 715108 714161 -316
(316 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR060w [I] KOG0873 C-4 sterol methyl oxidase 541 e-154
SPAC630.08c [I] KOG0873 C-4 sterol methyl oxidase 383 e-106
Hs5803157 [I] KOG0873 C-4 sterol methyl oxidase 187 2e-47
At1g07420 [I] KOG0873 C-4 sterol methyl oxidase 186 5e-47
At2g29390 [I] KOG0873 C-4 sterol methyl oxidase 179 4e-45
At4g12110 [I] KOG0873 C-4 sterol methyl oxidase 164 2e-40
At4g22750_1 [I] KOG0873 C-4 sterol methyl oxidase 154 1e-37
Hs4502499 [I] KOG0873 C-4 sterol methyl oxidase 112 7e-25
CE03385 [I] KOG0873 C-4 sterol methyl oxidase 97 3e-20
7292879 [I] KOG0873 C-4 sterol methyl oxidase 95 2e-19
CE03386 [I] KOG0873 C-4 sterol methyl oxidase 91 3e-18
SPBC887.15c [I] KOG0874 Sphingolipid hydroxylase 89 6e-18
CE04497 [I] KOG0873 C-4 sterol methyl oxidase 89 8e-18
7292910 [I] KOG0873 C-4 sterol methyl oxidase 88 1e-17
At1g69640 [I] KOG0874 Sphingolipid hydroxylase 79 9e-15
At1g14290 [I] KOG0874 Sphingolipid hydroxylase 79 9e-15
YDR297w [I] KOG0874 Sphingolipid hydroxylase 70 4e-12
Hs5902070 [I] KOG0872 Sterol C5 desaturase 70 5e-12
Hs20558831 [I] KOG0872 Sterol C5 desaturase 70 5e-12
SPAC1687.16c [I] KOG0872 Sterol C5 desaturase 52 9e-07
YLR056w [I] KOG0872 Sterol C5 desaturase 50 3e-06
>YGR060w [I] KOG0873 C-4 sterol methyl oxidase
Length = 309
Score = 541 bits (1393), Expect = e-154
Identities = 243/289 (84%), Positives = 262/289 (90%), Gaps = 3/289 (1%)
Query: 6 MSSVFSNGTIAELVGQGQGLSYGQVLSNIKVVQPQLNFMEQYWAAWYCYMNNDILATGLM 65
MS+VF+N T++ LV Q +Y Q L N+ QPQLNFME+YWAAWY YMNND+LATGLM
Sbjct: 1 MSAVFNNATLSGLV---QASTYSQTLQNVAHYQPQLNFMEKYWAAWYSYMNNDVLATGLM 57
Query: 66 FFLLHEFMYFFRCLPWAIIDQIPYFRRWKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPI 125
FFLLHEFMYFFRCLPW IIDQIPYFRRWK+QPTKIPSAKEQL+CLKSVLLSHFLVEAIPI
Sbjct: 58 FFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTKIPSAKEQLYCLKSVLLSHFLVEAIPI 117
Query: 126 WTFHPMCQKLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHH 185
WTFHPMC+KLGI+V VPFPSL TMA +I LFF LED WHYW HRLFHYGVFYKYIHKQHH
Sbjct: 118 WTFHPMCEKLGITVEVPFPSLKTMALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHH 177
Query: 186 RYAAPFGLAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHS 245
RYAAPFGL+AEYAHP ETLSLGFGTVGMPI YVMYTG LHLFTLC+WIT+RLFQAVDSHS
Sbjct: 178 RYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHS 237
Query: 246 GYDFPWSLNKFLPFWSGAEHHDLHHHYFIGNYASSFRWWDYVLDTEAGP 294
GYDFPWSLNK +PFW+GAEHHDLHHHYFIGNYASSFRWWDY LDTE+GP
Sbjct: 238 GYDFPWSLNKIMPFWAGAEHHDLHHHYFIGNYASSFRWWDYCLDTESGP 286
>SPAC630.08c [I] KOG0873 C-4 sterol methyl oxidase
Length = 300
Score = 383 bits (983), Expect = e-106
Identities = 169/280 (60%), Positives = 212/280 (75%), Gaps = 4/280 (1%)
Query: 12 NGTIAELVGQGQGLSYGQVLSNIKVVQPQLNFMEQYWAAWYCYMNNDILATGLMFFLLHE 71
N T +VG G + + + + P+LNF+EQ W A+Y + +ND++ATGLM FLLHE
Sbjct: 2 NTTSEVIVGTG----FQAIRQQLAQMHPELNFVEQLWLAYYKWFDNDVVATGLMSFLLHE 57
Query: 72 FMYFFRCLPWAIIDQIPYFRRWKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIWTFHPM 131
+YF RC+PW IID +PYFRRWKIQP K+P+ EQ C + VLLSHF VE IW F PM
Sbjct: 58 LIYFGRCIPWMIIDAMPYFRRWKIQPKKVPTLAEQWECTRLVLLSHFTVELPQIWLFDPM 117
Query: 132 CQKLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPF 191
C G+S +VPFP +T M QI+LFFFLED WHYW HRLFHYG+FY++IHK HHRY+APF
Sbjct: 118 CATFGLSTSVPFPPVTKMIWQITLFFFLEDTWHYWAHRLFHYGIFYRFIHKVHHRYSAPF 177
Query: 192 GLAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPW 251
GL+AEYAHP+E + LG GTV +P+ + +T +LHL T+ +WIT+RLFQAVDSH+GYDFPW
Sbjct: 178 GLSAEYAHPLEIILLGAGTVFVPLMWCYFTHDLHLVTMYIWITLRLFQAVDSHAGYDFPW 237
Query: 252 SLNKFLPFWSGAEHHDLHHHYFIGNYASSFRWWDYVLDTE 291
SLNKFLP W+GA+HHD HH F N++SSFRWWD VL T+
Sbjct: 238 SLNKFLPIWAGADHHDYHHMAFKDNFSSSFRWWDAVLKTD 277
>Hs5803157 [I] KOG0873 C-4 sterol methyl oxidase
Length = 293
Score = 187 bits (474), Expect = 2e-47
Identities = 98/260 (37%), Positives = 142/260 (53%), Gaps = 14/260 (5%)
Query: 39 PQLNFMEQYWAAWYCYMNN----DILATGLMFFLLHEFMYFFRCLPWAIIDQIPYFRRWK 94
P+ E + AW +NN I G + ++HE +YF CLP + IPY +++K
Sbjct: 25 PENPLQEPFKNAWNYMLNNYTKFQIATWGSL--IVHEALYFLFCLPGFLFQFIPYMKKYK 82
Query: 95 IQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVP-FPSLTTMAKQI 153
IQ K + + Q C K +L +HF ++ I + + I P + +
Sbjct: 83 IQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARC 142
Query: 154 SLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGT-VG 212
+ED WHY+ HRL H+ YKYIHK HH + APFG+ AEYAHP+ETL LG G +G
Sbjct: 143 FGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIG 202
Query: 213 MPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHHY 272
+ + H+ L W+T+RL + +D HSGYD P + +PF++G+ HHD HH
Sbjct: 203 IVLLCD------HVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 273 FIGNYASSFRWWDYVLDTEA 292
FIGNYAS+F WWD + T++
Sbjct: 257 FIGNYASTFTWWDRIFGTDS 276
>At1g07420 [I] KOG0873 C-4 sterol methyl oxidase
Length = 266
Score = 186 bits (471), Expect = 5e-47
Identities = 98/255 (38%), Positives = 146/255 (56%), Gaps = 10/255 (3%)
Query: 42 NFMEQYWAAWYCYMNNDILATGLMFFLLHEFMYFFRCLPWAIIDQIPYFRRWKIQPTKIP 101
+F+E W + ++ LA + FLLHE ++F LP+ +++ + ++KIQ TK
Sbjct: 3 SFVESGWQYLVTHFSDFQLAC-IGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQ-TKNN 60
Query: 102 SAKEQLFCLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTTMAKQISLFFFLED 161
+ Q C+ +LL HF V + +P+ + +G+ + P PS ++ QI +F +ED
Sbjct: 61 TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120
Query: 162 AWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVGMPIFYVMYT 221
YW HR+ H YK +H HH YA PFGL +EYAHP E L LGF T+ P T
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----T 176
Query: 222 GNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHHYFI---GNYA 278
G HL TL LW+ +R+ + V++H GY FPWSL+ FLP + GA+ HD HH GNY+
Sbjct: 177 GP-HLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYS 235
Query: 279 SSFRWWDYVLDTEAG 293
S+F + D++ T+ G
Sbjct: 236 STFVYMDWIFGTDKG 250
>At2g29390 [I] KOG0873 C-4 sterol methyl oxidase
Length = 253
Score = 179 bits (455), Expect = 4e-45
Identities = 90/230 (39%), Positives = 133/230 (57%), Gaps = 9/230 (3%)
Query: 67 FLLHEFMYFFRCLPWAIIDQIPYFRRWKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIW 126
F+LHE ++F LP+ +++ + +KIQ TK + + Q C+ +LL H V +
Sbjct: 20 FILHESVFFLSGLPYIFLERTGFLSNYKIQ-TKSNTPEAQGKCIARLLLYHCCVNLPLMM 78
Query: 127 TFHPMCQKLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHR 186
+P+ + +G+ + P PS ++ QI +F +ED YW HR+ H YK +H HH
Sbjct: 79 ASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHE 138
Query: 187 YAAPFGLAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSG 246
YA PFGL +EYAHP E L LGF T+ P TG HL TL LW+ +R+ + V++H G
Sbjct: 139 YATPFGLTSEYAHPAEILFLGFATIVGPAL----TGP-HLITLWLWMMLRVIETVEAHCG 193
Query: 247 YDFPWSLNKFLPFWSGAEHHDLHHHYFI---GNYASSFRWWDYVLDTEAG 293
Y FPWS + FLP + GA+ HD HH GNY+S+F + D++ T+ G
Sbjct: 194 YHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKG 243
>At4g12110 [I] KOG0873 C-4 sterol methyl oxidase
Length = 298
Score = 164 bits (415), Expect = 2e-40
Identities = 85/208 (40%), Positives = 115/208 (54%), Gaps = 10/208 (4%)
Query: 90 FRRWKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTTM 149
F R+KIQP S + C K V+ LV +P Q + I +P P++T M
Sbjct: 69 FNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEM 128
Query: 150 AKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFG 209
Q+ ++F +ED +YW HR FH Y IH+ HH Y AP G AA YAH E L L
Sbjct: 129 LSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLL--- 185
Query: 210 TVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLH 269
G+P F H+ T LWI +R +A+++HSGYDFPWS K++PF+ GAE+HD +
Sbjct: 186 --GIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYHD-Y 242
Query: 270 HHYFIG----NYASSFRWWDYVLDTEAG 293
HHY G N+AS F + DY+ T+ G
Sbjct: 243 HHYVGGQSQSNFASVFTYCDYIYGTDKG 270
>At4g22750_1 [I] KOG0873 C-4 sterol methyl oxidase
Length = 524
Score = 154 bits (390), Expect = 1e-37
Identities = 99/278 (35%), Positives = 138/278 (49%), Gaps = 40/278 (14%)
Query: 41 LNFMEQYW-------AAWYCYMNNDILATGLMFFLLHEFMYFFRCLPWAIIDQIP----Y 89
L ++E W + +Y Y +N IL L+F L+ LP I+
Sbjct: 18 LTWLETLWFDYSATKSDYYLYCHN-ILFLFLIFSLVP--------LPLVFIESSQSTSDL 68
Query: 90 FRRWKIQPTKIPSAKEQLFCLKSVLLSHFLV---------EAIPI-WTFHPMCQKLGISV 139
F R+KIQP S C K V+ LV +I + + F + Q + I
Sbjct: 69 FNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQVDFVFRVLKQMIEIRS 128
Query: 140 AVPFPSLTTMAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAH 199
+P PS + Q+ ++F +ED +YW HR FH Y+ H HH Y AP G AA YAH
Sbjct: 129 GLPLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAH 188
Query: 200 PVETLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPF 259
E L LG +P F H+ T LWI +R +A+++HSGYDFPWSL K++PF
Sbjct: 189 WAEVLLLG-----IPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPF 243
Query: 260 WSGAEHHDLHHHYFIG----NYASSFRWWDYVLDTEAG 293
+ GAE+HD +HHY G N+AS F + DY+ T+ G
Sbjct: 244 YGGAEYHD-YHHYVGGQSQSNFASVFTYCDYIYGTDKG 280
Score = 152 bits (383), Expect = 8e-37
Identities = 79/207 (38%), Positives = 116/207 (55%), Gaps = 11/207 (5%)
Query: 90 FRRWKIQPTKIPSAKEQLF-CLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTT 148
F++ +Q K+ + +F C K V+ LV +P Q +GI +P PSL
Sbjct: 283 FQKKLLQQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPLPSLME 342
Query: 149 MAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGF 208
+ Q+ ++F +ED +YW HR H Y+ IH+ HH Y +P G A+ YAH E L L
Sbjct: 343 IVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEILIL-- 400
Query: 209 GTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDL 268
G+P F H+ T LWI++R F+A+++HSGYDFPWS+ K +PF+ G E+HD
Sbjct: 401 ---GIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHD- 456
Query: 269 HHHYFIG----NYASSFRWWDYVLDTE 291
+HHY G N+AS F + DY+ T+
Sbjct: 457 YHHYVGGQSQSNFASVFTYCDYIYGTD 483
>Hs4502499 [I] KOG0873 C-4 sterol methyl oxidase
Length = 272
Score = 112 bits (280), Expect = 7e-25
Identities = 72/220 (32%), Positives = 107/220 (47%), Gaps = 15/220 (6%)
Query: 78 CLPWAIID----QIPYFRRWKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIWTFHPMCQ 133
CLP+ ++D +P RR+KI P PSA++ L CL L H + P+ H
Sbjct: 52 CLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMF-VFPVTLLH--WA 108
Query: 134 KLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHRLFHYGVF-YKYIHKQHHRYAAPFG 192
+ + P L + I L D + +H L H + Y+ HK HH+ ++ F
Sbjct: 109 RSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFA 168
Query: 193 LAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWS 252
LA +Y E SLGF + V G H T + + ++ +V+ HSGY+FPWS
Sbjct: 169 LATQYMSVWELFSLGF----FDMMNVTLLG-CHPLTTLTFHVVNIWLSVEDHSGYNFPWS 223
Query: 253 LNKFLPF--WSGAEHHDLHHHYFIGNYASSFRWWDYVLDT 290
++ +PF + G HHDLHH +F N+A F WD +L T
Sbjct: 224 THRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGT 263
>CE03385 [I] KOG0873 C-4 sterol methyl oxidase
Length = 269
Score = 97.1 bits (240), Expect = 3e-20
Identities = 57/198 (28%), Positives = 88/198 (43%), Gaps = 12/198 (6%)
Query: 93 WKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTTMAKQ 152
+KIQ K P + L K +L + F+ + ++ G S P PS + +
Sbjct: 53 YKIQDEKKPPLSKYLGAFKVILANQFIAGPLITLFWYFPAVWFGTSFTGPLPSGWQIFRD 112
Query: 153 ISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVG 212
I + E+ Y+ HRLFH+ YKYIHK+HH + AP + + Y HP+E
Sbjct: 113 IVISILCEEIGFYYSHRLFHHPKIYKYIHKKHHEWTAPVSITSIYCHPLEHAISNLS--- 169
Query: 213 MPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHHY 272
P+ H+ TL +W ++ + SHSGY FP+ L+ E HD HH
Sbjct: 170 -PVLLGPTICGSHVITLWIWASIAILSTTCSHSGYHFPFMLS--------PEPHDYHHKV 220
Query: 273 FIGNYASSFRWWDYVLDT 290
F + + W + D+
Sbjct: 221 FNECFGTGLLDWIHGTDS 238
>7292879 [I] KOG0873 C-4 sterol methyl oxidase
Length = 406
Score = 94.7 bits (234), Expect = 2e-19
Identities = 56/185 (30%), Positives = 93/185 (50%), Gaps = 14/185 (7%)
Query: 91 RRWKIQP-TKIPSAKEQLFCLKSVLLSHFLVEAIPI-WTFHPMCQKLGISVAVPFPSLTT 148
R++KIQP T P +L + ++ + + IP+ + + + + G+S P+
Sbjct: 186 RKYKIQPGTNEPVEVRRLLKVIWCVVCNQIFVGIPLAYASYRLMEIRGLSDIRDLPTFHW 245
Query: 149 MAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGF 208
+ ++++ +E+ Y+ HRL H+ YKYIHKQHH + AP + A Y HP+E + F
Sbjct: 246 VCFELTVCILMEELGFYYSHRLLHHKHIYKYIHKQHHEWTAPISVTAIYCHPIEHI---F 302
Query: 209 GTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDL 268
+ P V G+ H+ T LW + + +++HSGY LPF+ E HD
Sbjct: 303 SNLLPPFLGVFLMGS-HVATAWLWFALAILSTLNAHSGY--------HLPFFPSPEAHDF 353
Query: 269 HHHYF 273
HH F
Sbjct: 354 HHLKF 358
>CE03386 [I] KOG0873 C-4 sterol methyl oxidase
Length = 286
Score = 90.5 bits (223), Expect = 3e-18
Identities = 54/181 (29%), Positives = 85/181 (46%), Gaps = 12/181 (6%)
Query: 93 WKIQPTKIPSAKEQLFCLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTTMAKQ 152
+KIQ K PS + L LK V + +V I + + + G+ PS + +
Sbjct: 54 YKIQEEKKPSLSKYLSILKVVGPNQLIVTPIVTTLWFYVARWWGMDFGPVIPSWYILLRD 113
Query: 153 ISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVG 212
L +++ Y+ HRLFH+ YK+IHK+HH + AP +++ YAHP+E
Sbjct: 114 ACLCMAMDEIGFYYTHRLFHHPKLYKHIHKKHHEWNAPVSISSIYAHPLEHAISNLS--- 170
Query: 213 MPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHHY 272
PI+ H+ + ++ + + HSGY FP+ L+ AEHHD HH
Sbjct: 171 -PIYLGAVLFRCHVVSHYIFTSYAILTTTFHHSGYHFPFMLS--------AEHHDFHHKV 221
Query: 273 F 273
F
Sbjct: 222 F 222
>SPBC887.15c [I] KOG0874 Sphingolipid hydroxylase
Length = 293
Score = 89.4 bits (220), Expect = 6e-18
Identities = 60/170 (35%), Positives = 81/170 (47%), Gaps = 18/170 (10%)
Query: 130 PMCQKLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHRLFHYGV-FYKYIHKQHHRYA 188
P KL + VP A Q FF+ D+W Y++HR HY Y IH HHR
Sbjct: 118 PFAPKLAYNFIVP-------AFQYFFAFFIIDSWQYFWHRYLHYNKKLYNMIHAHHHRLQ 170
Query: 189 APFGLAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYD 248
P+ + A Y HP E L L T G + Y+ L ++ T+ + VD H GY
Sbjct: 171 VPYAMGALYNHPFEGLIL--DTFGAGVAYL--AAGLSPQQAVIFFTLSTLKTVDDHCGYV 226
Query: 249 FPWS-LNKFLPFWSGAEHHDLHH--HYFIGNYASS-FRWWDYVLDTEAGP 294
FP+ L F F + A +HDLHH + F N++ F +WD+VL T P
Sbjct: 227 FPYDPLQMF--FANNARYHDLHHQPYGFQKNFSQPFFTFWDHVLGTYMPP 274
>CE04497 [I] KOG0873 C-4 sterol methyl oxidase
Length = 300
Score = 89.0 bits (219), Expect = 8e-18
Identities = 41/128 (32%), Positives = 70/128 (54%), Gaps = 6/128 (4%)
Query: 144 PSLTTMAKQISLFFFLEDAWHYWFHRLFHYGVF-YKYIHKQHHRYAAPFGLAAEYAHPVE 202
P++ M Q+++FF D ++WFH + H + Y++ H HH Y++PF +A++ HP E
Sbjct: 125 PTVFEMLSQLAIFFLAFDFTYFWFHYINHKVKWLYRWCHSVHHMYSSPFAASAQHLHPFE 184
Query: 203 TLSLGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSG 262
+G +P + H T +W + + + H GYDFP++L++ F+SG
Sbjct: 185 LFFVGTFITTIPWIFPT-----HCLTYWIWFFIAQSVSYEVHIGYDFPFALHRIFWFYSG 239
Query: 263 AEHHDLHH 270
A HD+HH
Sbjct: 240 APAHDMHH 247
>7292910 [I] KOG0873 C-4 sterol methyl oxidase
Length = 278
Score = 88.2 bits (217), Expect = 1e-17
Identities = 60/236 (25%), Positives = 106/236 (44%), Gaps = 22/236 (9%)
Query: 45 EQYWAAWYCYMNNDILATGLMFFLLHEFMYFFRCLPWAIIDQIPYFRRWKIQPTKI-PSA 103
E+ W W ++ + ++ F+++ ++Y I ++ + R++KIQP + P
Sbjct: 28 EESWRLW-------VVGSTVVIFIVY-WLYAGIFTLMDITNRPRFLRKYKIQPGQNEPVN 79
Query: 104 KEQLF-CLKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVP-FPSLTTMAKQISLFFFLED 161
+L+ +K VL + +V + W + K S + P+ + + +F LE+
Sbjct: 80 LAKLWHAVKVVLFNLTVVNFLVSWVVYEFVYKSENSQDIRVLPTFKRSLRDLVVFVVLEE 139
Query: 162 AWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVGMPIFYVMYT 221
Y+ HRL H+ YKY+HK+HH + AP YAHPVE + +P+ +
Sbjct: 140 IMFYYAHRLLHHRSVYKYVHKKHHEWTAPIAAITLYAHPVEHVLANL----LPVATSIAI 195
Query: 222 GNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHHYFIGNY 277
H+ + + + ++ H+GY FPWS HD HH F NY
Sbjct: 196 LGTHVALAYVIFALAIINSMSDHTGYSFPWSA-------GSVRFHDYHHAKFNYNY 244
>At1g69640 [I] KOG0874 Sphingolipid hydroxylase
Length = 258
Score = 79.0 bits (193), Expect = 9e-15
Identities = 55/185 (29%), Positives = 82/185 (43%), Gaps = 10/185 (5%)
Query: 110 LKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHR 169
+K VL+ + + I F A F S +A+Q + D W Y+ HR
Sbjct: 55 VKGVLVQQVVQAVVAILLFTVTGSDAEADKAQQF-SFLVLARQFVTAMIVLDTWQYFMHR 113
Query: 170 LFHYGVF-YKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFT 228
H F YK+IH QHHR P+ A Y HPVE L L T+G + +++ + T
Sbjct: 114 YMHQNKFLYKHIHSQHHRLIVPYAYGALYNHPVEGLLL--DTIGGALSFLV--SGMSPRT 169
Query: 229 LCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHHYFIGNYASS---FRWWD 285
+ + + VD H G P +L + F + + +HD+HH + Y S F WD
Sbjct: 170 SIFFFSFATIKTVDDHCGLWLPGNLFHMV-FKNNSAYHDIHHQLYGTKYNFSQPFFVMWD 228
Query: 286 YVLDT 290
+L T
Sbjct: 229 RILGT 233
>At1g14290 [I] KOG0874 Sphingolipid hydroxylase
Length = 258
Score = 79.0 bits (193), Expect = 9e-15
Identities = 57/186 (30%), Positives = 83/186 (43%), Gaps = 12/186 (6%)
Query: 110 LKSVLLSHFLVEAIPIWTFHPMCQKLGISVAVPFPSLTTMAKQISLFFFLEDAWHYWFHR 169
+K VLL L I + F + F S+ +A+Q + + D W Y+ HR
Sbjct: 55 VKGVLLQQTLQAIISVILFKITGSDADAATTQQF-SILLLARQFIIAMLVIDTWQYFIHR 113
Query: 170 LFHYGVF-YKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVGMPIFYVMYTGNLHLFT 228
H F YK+IH QHHR P+ A Y HP+E L L T+G + ++ + T
Sbjct: 114 YMHLNKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLL--DTIGGALSFLF--SGMSPRT 169
Query: 229 LCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWS-GAEHHDLHHHYFIGNYASS---FRWW 284
+ + + VD H G P N F F+S + +HD+HH + Y S F W
Sbjct: 170 AIFFFSFATIKTVDDHCGLWLPG--NPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMW 227
Query: 285 DYVLDT 290
D +L T
Sbjct: 228 DRILGT 233
>YDR297w [I] KOG0874 Sphingolipid hydroxylase
Length = 349
Score = 70.1 bits (170), Expect = 4e-12
Identities = 49/148 (33%), Positives = 72/148 (48%), Gaps = 15/148 (10%)
Query: 150 AKQISLFFFLEDAWHYWFHRLFHYG-VFYKYIHKQHHRYAAPFGLAAEYAHPVETL---S 205
A +I F D W Y+ HRL H YK+ H HH P+ A + +PVE +
Sbjct: 158 ALKIFAGFLFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPYAYGALFNNPVEGFLLDT 217
Query: 206 LGFGTVGMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEH 265
LG G + M + ++ + + LFT + TM+ VD H GY P ++L F + A +
Sbjct: 218 LGTG-IAMTLTHLTHREQIILFT---FATMK---TVDDHCGYALPLDPFQWL-FPNNAVY 269
Query: 266 HDLHHHYF--IGNYASS-FRWWDYVLDT 290
HD+HH F N+A F +WD + T
Sbjct: 270 HDIHHQQFGIKTNFAQPFFTFWDNLFQT 297
>Hs5902070 [I] KOG0872 Sterol C5 desaturase
Length = 299
Score = 69.7 bits (169), Expect = 5e-12
Identities = 47/148 (31%), Positives = 69/148 (45%), Gaps = 12/148 (8%)
Query: 143 FP-SLTTMAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPV 201
FP L + I F F D + YW HR H+ + YK +HK HH + P A+ HP+
Sbjct: 112 FPYGLFELVVSIISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPI 171
Query: 202 ETLSLGFGTVGMPIFYVMYTGNLH-LFTLCLWITMRLFQAVDSHSG-YDFPWSLNKFLPF 259
+ GF +P + LH + L L+I + ++ + H G + P L PF
Sbjct: 172 D----GF-LQSLPYHIYPFIFPLHKVVYLSLYILVNIW-TISIHDGDFRVPQILQ---PF 222
Query: 260 WSGAEHHDLHHHYFIGNYASSFRWWDYV 287
+G+ HH HH +F NY F WD +
Sbjct: 223 INGSAHHTDHHMFFDYNYGQYFTLWDRI 250
>Hs20558831 [I] KOG0872 Sterol C5 desaturase
Length = 299
Score = 69.7 bits (169), Expect = 5e-12
Identities = 47/148 (31%), Positives = 69/148 (45%), Gaps = 12/148 (8%)
Query: 143 FP-SLTTMAKQISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPV 201
FP L + I F F D + YW HR H+ + YK +HK HH + P A+ HP+
Sbjct: 112 FPYGLFELVVSIISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPI 171
Query: 202 ETLSLGFGTVGMPIFYVMYTGNLH-LFTLCLWITMRLFQAVDSHSG-YDFPWSLNKFLPF 259
+ GF +P + LH + L L+I + ++ + H G + P L PF
Sbjct: 172 D----GF-LQSLPYHIYPFIFPLHKVVYLSLYILVNIW-TISIHDGDFRVPQILQ---PF 222
Query: 260 WSGAEHHDLHHHYFIGNYASSFRWWDYV 287
+G+ HH HH +F NY F WD +
Sbjct: 223 INGSAHHTDHHMFFDYNYGQYFTLWDRI 250
>SPAC1687.16c [I] KOG0872 Sterol C5 desaturase
Length = 300
Score = 52.4 bits (124), Expect = 9e-07
Identities = 37/128 (28%), Positives = 52/128 (39%), Gaps = 16/128 (12%)
Query: 153 ISLFFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVG 212
+ LF D YW HR H+ Y +HK HH++ + YA + GF
Sbjct: 122 LPLFVMFSDFGIYWAHRFLHHRYVYPRLHKLHHKWI----ICTPYASHAFKSADGF-LQS 176
Query: 213 MPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFL---PFWSGAEHHDLH 269
+P + LH T T F ++ H G K++ P +GA HH+ H
Sbjct: 177 LPYHLFPFFFPLHKLTYLALFTFVNFWSIMIHDG--------KYISNNPIINGAAHHNGH 228
Query: 270 HHYFIGNY 277
H YF NY
Sbjct: 229 HIYFNYNY 236
>YLR056w [I] KOG0872 Sterol C5 desaturase
Length = 365
Score = 50.4 bits (119), Expect = 3e-06
Identities = 38/134 (28%), Positives = 57/134 (42%), Gaps = 18/134 (13%)
Query: 156 FFFLEDAWHYWFHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPV----ETLSLGFGTV 211
F F D Y HR H+ Y+ +HK HH++ A+ HPV +++S +
Sbjct: 188 FIFFTDCGVYLAHRWLHWPRVYRALHKPHHKWLVCTPFASHSFHPVDGFLQSISYHIYPL 247
Query: 212 GMPIFYVMYTGNLHLFTLCLWITMRLFQAVDSHSGYDFPWSLNKFLPFWSGAEHHDLHHH 271
+P+ V Y L LFT + T+ + +D + N P +G H +HH
Sbjct: 248 ILPLHKVSY---LILFTFVNFWTVMI---------HDGQYLSNN--PAVNGTACHTVHHL 293
Query: 272 YFIGNYASSFRWWD 285
YF NY WD
Sbjct: 294 YFNYNYGQFTTLWD 307
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.330 0.142 0.491
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,600,802
Number of Sequences: 60738
Number of extensions: 796824
Number of successful extensions: 2131
Number of sequences better than 1.0e-05: 21
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2075
Number of HSP's gapped (non-prelim): 23
length of query: 316
length of database: 30,389,216
effective HSP length: 106
effective length of query: 210
effective length of database: 23,950,988
effective search space: 5029707480
effective search space used: 5029707480
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)