ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactII2180   suspect: LH   O     KOG2177  Posttranslational modification, protein turnover, chaperones   Predicted E3 ubiquitin ligase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactII2180 767808  768896 363  
         (363 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOL054w [O] KOG2177 Predicted E3 ubiquitin ligase                     170   3e-42

>YOL054w [O] KOG2177 Predicted E3 ubiquitin ligase
          Length = 406

 Score =  170 bits (430), Expect = 3e-42
 Identities = 115/361 (31%), Positives = 158/361 (42%), Gaps = 53/361 (14%)

Query: 1   MNGHLHTVIPKKSNADHLRIVGRVLDSTVCTICHDYMFVPVMTSCGHXXXXXXXXXXXXX 60
           M   LH  +  +++     I+ ++++S VC+ICHDYMFVP+MT CGH             
Sbjct: 1   MGDELHNRLLHQNDGTKDAILYKIIESLVCSICHDYMFVPMMTPCGHNYCYGCLNTWFAS 60

Query: 61  XXXXXXXLTCPQCRNVITNEPSLNVALQQIVDLMLDIAAEDPQKSNSSEVRSLLTQRECS 120
                  L CPQCR+ IT  P+LN  LQQ +  +L    E  +  N    + LLT +   
Sbjct: 61  NTQKE--LACPQCRSDITTIPALNTTLQQYLSFIL----EKLRDQNDESFKKLLTTKTKE 114

Query: 121 VAEYKSDLLEETLFNSVFKNTAKAXXXXXXXGIARCSNCQWEV------EGPVCPNCNAT 174
             +YK+D  ++TLF+ VFKN+A A       GI RCSNC WE+      +G VCP+CNA 
Sbjct: 115 ENDYKNDKEKDTLFDKVFKNSALAVADDSDDGITRCSNCHWELDPDEVEDGNVCPHCNAR 174

Query: 175 IRNYNAGAQNFDEVDVDEYSDGELEEVERDLDNYRAESARTVH------DLEAEEGDESL 228
           IRNY  G   FDE   +EYS+GEL+E+   +   R     + +      D+ +E+ D S 
Sbjct: 175 IRNYAGGRDEFDE---EEYSEGELDEIRESMRRRRENRFASTNPFANRDDVSSEDDDSSE 231

Query: 229 SSEMDQAWPTRRPRNFMHGXXXXXXXXDR--------------GSIDSFIVDXXXXXXXX 274
              M +  P  R     +         D                 +  FI D        
Sbjct: 232 EEPMREHIPLGRWARSHNRSIAVDAVDDEDDEEEDEEEEEEMDSDLKDFIEDDEDDEDED 291

Query: 275 XXXXXL-------------KDERKFRRASVALXXXXXXXXXXXXFYEHNDDGYASGDSLD 321
                L              DE + +R                 FYEHNDDG+ SGDSLD
Sbjct: 292 GSRRNLVLSALKNRHVIITDDEEEEQR-----RHATEEEDRDSDFYEHNDDGFVSGDSLD 346

Query: 322 E 322
           E
Sbjct: 347 E 347


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.315    0.131    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,695,413
Number of Sequences: 60738
Number of extensions: 614816
Number of successful extensions: 2449
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2443
Number of HSP's gapped (non-prelim): 2
length of query: 363
length of database: 30,389,216
effective HSP length: 108
effective length of query: 255
effective length of database: 23,829,512
effective search space: 6076525560
effective search space used: 6076525560
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)