ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2292 suspect: HSP K KOG2150 Transcription CCR4-NOT transcriptional regulation complex, NOT5 subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2292 805031 803262 -590
(590 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR072w [K] KOG2150 CCR4-NOT transcriptional regulation complex ... 278 2e-74
SPAC1B3.05 [K] KOG2150 CCR4-NOT transcriptional regulation compl... 131 3e-30
YIL038c [K] KOG2150 CCR4-NOT transcriptional regulation complex ... 122 2e-27
Hs7657387 [K] KOG2150 CCR4-NOT transcriptional regulation comple... 122 2e-27
7302229 [K] KOG2150 CCR4-NOT transcriptional regulation complex ... 120 4e-27
At5g18230 [K] KOG2150 CCR4-NOT transcriptional regulation comple... 117 4e-26
CE22572 [K] KOG2150 CCR4-NOT transcriptional regulation complex ... 94 4e-19
CE18516 [K] KOG2150 CCR4-NOT transcriptional regulation complex ... 60 7e-09
ECU06g1510 [K] KOG2150 CCR4-NOT transcriptional regulation compl... 55 4e-07
>YPR072w [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 560
Score = 278 bits (710), Expect = 2e-74
Identities = 131/191 (68%), Positives = 159/191 (82%)
Query: 26 DIYEKFQNTESDNQSYREKLESDLKREIKKLQKHREQIKNWLSKDDIKDRADVLMENRRL 85
DIYEKFQ+T+ N S+REKLESDLKREIKKLQKHR+QIK WLSK+D+KD+ VLM NRRL
Sbjct: 26 DIYEKFQSTDPSNSSHREKLESDLKREIKKLQKHRDQIKTWLSKEDVKDKQSVLMTNRRL 85
Query: 86 IESGMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEELQKQLDTF 145
IE+GMERFKS+EK+MKTK+FS EALTNPD+IKDP+ELKKRDQ LF+ +CL+ELQKQL+ +
Sbjct: 86 IENGMERFKSVEKLMKTKQFSKEALTNPDIIKDPKELKKRDQVLFIHDCLDELQKQLEQY 145
Query: 146 EVENNEEQIEKHTFHISNLENILKLLQNDDLDPETVQEFQEDIRXXXXXXXXXXXXXXXT 205
E + NEEQ E+H FHI+NLENILK LQN+++DPE V+EFQ+DI+ T
Sbjct: 146 EAQENEEQTERHEFHIANLENILKKLQNNEMDPEPVEEFQDDIKYYVENNDDPDFIEYDT 205
Query: 206 IYQDMGCEIQP 216
IY+DMGCEIQP
Sbjct: 206 IYEDMGCEIQP 216
Score = 272 bits (695), Expect = 1e-72
Identities = 135/250 (54%), Positives = 172/250 (68%), Gaps = 26/250 (10%)
Query: 364 VKKEKESTPAPQTEELPPPKDLSHEIEEILAKDLEDKLAFKNPLFKEELPYWLETKKKLI 423
+KK++ + Q PP D + EI + + D+E AF+NPLF +EL YWL++K+ L+
Sbjct: 313 MKKKESENDSEQQLNFPP--DRTDEIRKTIQHDVETNAAFQNPLFNDELKYWLDSKRYLM 370
Query: 424 QPPNEIDEFTLKHLESSLLNCPDSLDADTPRLFENPLSLPHPTSIFFPHEPIRFM-AQDL 482
QP E+ + LESSLLNCPDSLDAD+P L+ PLSLPHPTSIFFP+EPIRF+ D+
Sbjct: 371 QPLQEMSPKMVSQLESSLLNCPDSLDADSPCLYTKPLSLPHPTSIFFPNEPIRFVYPYDV 430
Query: 483 P----------------------AKGDDLYGKISVAKIMSKFALDTLFFIFYHYQGSYDQ 520
P K DD+Y + S+A+I KF LDTLFFIFYHYQGSY+Q
Sbjct: 431 PLNLTNNENDTDNKFGKDSKAKSKKDDDIYSRTSLARIFMKFDLDTLFFIFYHYQGSYEQ 490
Query: 521 FLASREL-SLRGWTFNKVNRCWFHKEVEKLPPGIEGKEEVTWRYFDYQKAWLSRRCGPDF 579
FLA+REL R W FNKV+RCW++KE+EKLPPG+ EE +WRYFDY+K+WL+RRCG DF
Sbjct: 491 FLAARELFKNRNWLFNKVDRCWYYKEIEKLPPGMGKSEEESWRYFDYKKSWLARRCGNDF 550
Query: 580 EYKEEEFEKL 589
Y EE+FEKL
Sbjct: 551 VYNEEDFEKL 560
>SPAC1B3.05 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 630
Score = 131 bits (330), Expect = 3e-30
Identities = 75/185 (40%), Positives = 110/185 (58%), Gaps = 26/185 (14%)
Query: 26 DIYEKFQNTESDNQSYREKLESDLKREIKKLQKHREQIKNWLSKDDIKDRADVLMENRRL 85
++YEK + S++ S +EKLE DLK +IKKLQ+ R+QIK W S +DIKD+ L+ENRRL
Sbjct: 16 EVYEKL--SASNSVSQKEKLEGDLKTQIKKLQRLRDQIKTWASSNDIKDK-KALLENRRL 72
Query: 86 IESGMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEELQKQLDTF 145
IE+ ME FK++E+ MK K FS E L+ + DP+E +K+D ++ +EEL++Q +
Sbjct: 73 IEAKMEEFKAVEREMKIKAFSKEGLSIASKL-DPKEKEKQDTIQWISNAVEELERQAELI 131
Query: 146 EVENN----------------------EEQIEKHTFHISNLENILKLLQNDDLDPETVQE 183
E E E +IE+H +H LE I++ L+N + PE V +
Sbjct: 132 EAEAESLKATFKRGKKDLSKLSHLSELESRIERHKWHQDKLELIMRRLENSQISPEAVND 191
Query: 184 FQEDI 188
QEDI
Sbjct: 192 IQEDI 196
Score = 71.2 bits (173), Expect = 4e-12
Identities = 58/224 (25%), Positives = 106/224 (46%), Gaps = 30/224 (13%)
Query: 370 STPAPQTEELPPPKDLSHEIEEILAKDLEDK--LAFKNPLFKEELPYWLETKKKLIQPPN 427
++P QT+ + S E + E+ + +P + ++L L T K+ Q
Sbjct: 424 ASPKMQTQPVRSVSQSSATTETNVKPTKEENADVPVSSPDYLKDLVNALNTSKE--QHKG 481
Query: 428 EIDEFTLKH-LESSLLNCPDSLDADTPRLF--ENPLSLPHPTSIFFPHEPIRFMAQDLPA 484
ID+ L L S + PD+ DA P+ + ++P +PH ++P +P+
Sbjct: 482 AIDKEKLTEALNISCVYVPDATDAAKPQYYIPKDPYPVPH----YYPQQPL--------- 528
Query: 485 KGDDLYGKISVAKIMSKFALDTLFFIFYHYQGSYDQFLASRELSLRGWTFNKVNRCWFHK 544
L+ + +++ DTLF++FY+ G+Y Q++A +EL + W F+K WF +
Sbjct: 529 ---PLFDSSEMTELVDP---DTLFYMFYYRPGTYQQYIAGQELKKQSWRFHKKYTTWFQR 582
Query: 545 EVEKLPPGIEGK-EEVTWRYFDYQKAWLSRRCGPDFEYKEEEFE 587
E P I + E ++RYFD++ W+ R+ DF + + E
Sbjct: 583 HEE--PKMITDEFESGSYRYFDFEGDWVQRK-KADFRFTYQYLE 623
>YIL038c [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 836
Score = 122 bits (306), Expect = 2e-27
Identities = 72/209 (34%), Positives = 120/209 (56%), Gaps = 26/209 (12%)
Query: 28 YEKFQNTESDNQSYREKLESDLKREIKKLQKHREQIKNWLSKDDIKDRADVLMENRRLIE 87
YE+ ++ ++N S ++KLESDLKRE+KKLQ+ REQIK+W S DIKD+ D L++ RR +E
Sbjct: 28 YERHESC-TNNPSQKDKLESDLKREVKKLQRLREQIKSWQSSPDIKDK-DSLLDYRRSVE 85
Query: 88 SGMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEELQKQLDTFEV 147
ME++K++EK K K +S +L + + DP+E ++RD ++ + ++EL++Q D+ +V
Sbjct: 86 IAMEKYKAVEKASKEKAYSNISLKKSETL-DPQERERRDISEYLSQMIDELERQYDSLQV 144
Query: 148 E----------------NNEEQIE-------KHTFHISNLENILKLLQNDDLDPETVQEF 184
E N+E+ E ++ +H +E L+LL N++LDP+ V+
Sbjct: 145 EIDKLLLLNKKKKTSSTTNDEKKEQYKRFQARYRWHQQQMELALRLLANEELDPQDVKNV 204
Query: 185 QEDIRXXXXXXXXXXXXXXXTIYQDMGCE 213
Q+DI TIY + +
Sbjct: 205 QDDINYFVESNQDPDFVEDETIYDGLNLQ 233
>Hs7657387 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 753
Score = 122 bits (305), Expect = 2e-27
Identities = 71/217 (32%), Positives = 118/217 (53%), Gaps = 26/217 (11%)
Query: 26 DIYEKFQNTESDNQSYREKLESDLKREIKKLQKHREQIKNWLSKDDIKDRADVLMENRRL 85
DI++K N + NQ +EK E+DLK+EIKKLQ+ R+QIK W++ ++IKD+ L++NR+L
Sbjct: 27 DIWQKLHNAANANQ--KEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQ-LIDNRKL 83
Query: 86 IESGMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEELQKQLDTF 145
IE+ MERFK +E+ KTK +S E L + DP + +K + ++ ++ L Q+D F
Sbjct: 84 IETQMERFKVVERETKTKAYSKEGLGLAQKV-DPAQKEKEEVGQWLTNTIDTLNMQVDQF 142
Query: 146 E----------------------VENNEEQIEKHTFHISNLENILKLLQNDDLDPETVQE 183
E +E + IEKH +H+ LE IL++L ND + + +++
Sbjct: 143 ESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRK 202
Query: 184 FQEDIRXXXXXXXXXXXXXXXTIYQDMGCEIQPEDLL 220
++D+ +Y D+ E P+ L+
Sbjct: 203 IKDDVEYYVDSSQDPDFEENEFLYDDLDLEDIPQALV 239
Score = 59.7 bits (143), Expect = 1e-08
Identities = 57/250 (22%), Positives = 106/250 (41%), Gaps = 29/250 (11%)
Query: 350 PPSSVNQISGANPVVKKEKESTPAPQTEELPPPKDLSHEIEEI-LAKDLEDKLAFKNPLF 408
P S + A ++ + + AP+ + P L E ++ +ED + +
Sbjct: 508 PTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTE 567
Query: 409 KEELPYWLETKKKLIQPPNEIDEFTLKHLESSLLNCP-DSLDADTPRLFENPLS------ 461
++ + QPP ++ E ++ SL CP + +L++ +
Sbjct: 568 RDIILSSTSAPPASAQPPLQLSEV---NIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHH 624
Query: 462 LPHPTSI-----FFPHEPIR---FMAQDLPAKGDDLYGKISVAKIMSKFALDTLFFIFYH 513
+PHP+ + P P + Q P D + + + + +TLFFIFY+
Sbjct: 625 MPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTV-------EFYQRLSTETLFFIFYY 677
Query: 514 YQGSYDQFLASRELSLRGWTFNKVNRCWFHKEVEKLPPGIEGK-EEVTWRYFDYQKAWLS 572
+G+ Q+LA++ L + W F+ WF + E P I + E+ T+ YFDY+K
Sbjct: 678 LEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEE--PKTITDEFEQGTYIYFDYEKWGQR 735
Query: 573 RRCGPDFEYK 582
++ G FEY+
Sbjct: 736 KKEGFTFEYR 745
>7302229 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 948
Score = 120 bits (302), Expect = 4e-27
Identities = 74/209 (35%), Positives = 116/209 (55%), Gaps = 26/209 (12%)
Query: 26 DIYEKFQNTESDNQSYR---EKLESDLKREIKKLQKHREQIKNWLSKDDIKDRADVLMEN 82
DI++K N + NQ + EK E+DLK+EIKKLQ+ R+QIK+W++ +IKD++ L+EN
Sbjct: 27 DIWKKVHNATNTNQKQKHLQEKYEADLKKEIKKLQRLRDQIKSWIASAEIKDKSS-LLEN 85
Query: 83 RRLIESGMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEELQKQL 142
RRLIE+ MERFK +E+ KTK +S E L + DP + K D ++ + LQ Q+
Sbjct: 86 RRLIETQMERFKVVERETKTKAYSKEGLGAAQKM-DPAQRIKDDARNWLTSSISSLQIQI 144
Query: 143 DTFEVE--------------NNEE-------QIEKHTFHISNLENILKLLQNDDLDPETV 181
D +E E + +E ++++H FHI+ LE +L+LL ND ++ E V
Sbjct: 145 DQYESEIESLLAGKKKRLDRDKQERMDDLRGKLDRHKFHITKLETLLRLLDNDGVEAEQV 204
Query: 182 QEFQEDIRXXXXXXXXXXXXXXXTIYQDM 210
+ ++D+ IY D+
Sbjct: 205 NKIKDDVEYYIDSSQEPDFEENEFIYDDI 233
Score = 59.7 bits (143), Expect = 1e-08
Identities = 33/91 (36%), Positives = 53/91 (57%), Gaps = 5/91 (5%)
Query: 501 KFALDTLFFIFYHYQGSYDQFLASRELSLRGWTFNKVNRCWFHKEVEKLPPGI--EGKEE 558
+ + +TLFF+FY+ +GS Q+LA++ L + W F+ WF + E P I + E+
Sbjct: 860 RLSTETLFFVFYYMEGSKAQYLAAKALKKQSWRFHTKYMMWFQRHEE---PKIINDDYEQ 916
Query: 559 VTWRYFDYQKAWLSRRCGPDFEYKEEEFEKL 589
T+ YFDY+K ++ G FEYK E ++L
Sbjct: 917 GTYIYFDYEKWSQRKKEGFTFEYKYLEDKEL 947
>At5g18230 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 889
Score = 117 bits (294), Expect = 4e-26
Identities = 71/186 (38%), Positives = 104/186 (55%), Gaps = 26/186 (13%)
Query: 26 DIYEKFQNT--ESDNQSYREKLESDLKREIKKLQKHREQIKNWLSKDDIKDR------AD 77
D+++ N ++DN + +EK E+DLK+EIKKLQ++R+QIK W+ +IKD+
Sbjct: 23 DVFDSIWNKVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ 82
Query: 78 VLMENRRLIESGMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEE 137
L++ R+LIE MERFK EK KTK FS E L DP+E K + ++ + E
Sbjct: 83 SLVDARKLIEKEMERFKICEKETKTKAFSKEGLGQQPK-TDPKEKAKSETRDWLNNVVSE 141
Query: 138 LQKQLDTFEVE-----------------NNEEQIEKHTFHISNLENILKLLQNDDLDPET 180
L+ Q+D+FE E + E I +H HI LE IL+LL ND+L PE
Sbjct: 142 LESQIDSFEAELEGLSVKKGKTRPPRLTHLETSITRHKDHIIKLELILRLLDNDELSPEQ 201
Query: 181 VQEFQE 186
V + ++
Sbjct: 202 VNDVKD 207
Score = 58.2 bits (139), Expect = 4e-08
Identities = 41/138 (29%), Positives = 61/138 (43%), Gaps = 21/138 (15%)
Query: 434 LKHLESSLLNCPDSLDADTPRLFE--NPLSLPHPTSIFFPHEPIRFMAQDL--PAKGDDL 489
L+ LE++ P D++ PR + NP P FP + L G D
Sbjct: 737 LQMLEAAFYKRPQPSDSERPRPYSPRNPAITPQT----FPQTQAPIINNPLLWERLGSDA 792
Query: 490 YGKISVAKIMSKFALDTLFFIFYHYQGSYDQFLASRELSLRGWTFNKVNRCWFHKEVEKL 549
YG DTLFF FY+ Q SY Q+LA++EL + W +++ WF + E
Sbjct: 793 YGT------------DTLFFAFYYQQNSYQQYLAAKELKKQSWRYHRKFNTWFQRHKEP- 839
Query: 550 PPGIEGKEEVTWRYFDYQ 567
+ E+ + YFD+Q
Sbjct: 840 KIATDEYEQGAYVYFDFQ 857
>CE22572 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 513
Score = 94.4 bits (233), Expect = 4e-19
Identities = 60/186 (32%), Positives = 96/186 (51%), Gaps = 35/186 (18%)
Query: 29 EKFQNTESDNQSYREKLESDLKREIKKLQKHREQIKNWLSKDDIKDRADVLMENRRLIES 88
EK SDNQ R+K + DLK+EIKKLQ+ R+Q+KNW + +IKD+ D L R+LIE
Sbjct: 30 EKMHEANSDNQ--RDKYQDDLKKEIKKLQRLRDQVKNWQNASEIKDK-DKLNSYRKLIEQ 86
Query: 89 GMERFKSIEKIMKTKKFSTEALTNPDLIKDPRELKKRDQFLFVEECLEELQKQLDTFE-- 146
ME+FK +E+ KTK S L+ + + DP+E +K + +++ + L +++D E
Sbjct: 87 RMEQFKDVERENKTKPHSKLGLSAEEKL-DPKEKEKAETMDWIQHQIRSLNEEVDRTEMQ 145
Query: 147 -----------------------------VENNEEQIEKHTFHISNLENILKLLQNDDLD 177
VE + +E+ FHI LE ++++ N+ L+
Sbjct: 146 LESLSNTDTGKGKRGKKEDAKTKNEREKRVEGLKHHLERINFHIEKLEICMRMISNESLN 205
Query: 178 PETVQE 183
+ V E
Sbjct: 206 AKMVLE 211
>CE18516 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 198
Score = 60.5 bits (145), Expect = 7e-09
Identities = 49/184 (26%), Positives = 83/184 (44%), Gaps = 25/184 (13%)
Query: 409 KEELPYWLETKKKLIQPPNEIDEFTLKHLESSLLNCPDSLDADTPRLFENPLSLPHPTSI 468
+ +P WL + + L LE + LD++ PR + + +S P P+
Sbjct: 35 RAHIPAWLGASPLGRTSMTQEFDGQLAALELACAKATFPLDSEKPRNYLSKVSFPVPS-- 92
Query: 469 FFPHEPIRFMAQDLPAKGDDLYGKISVAKIMSKFALDTLFFIFYHYQGSYDQFLASRELS 528
+ Q P D L + + A DTLFFIFY+ +G+ Q LA++ L
Sbjct: 93 --------WYGQTAPNTSDSL-------EYYLRLAPDTLFFIFYYMEGTRAQLLAAKALK 137
Query: 529 LRGWTFNKVNRCWF--HKEVEKLPPGIEGKEEVTWRYFDYQKAWLSRRCGPDFEYK---E 583
W F+ WF H+E +++ + E+ T+ YFD++K ++ FEYK +
Sbjct: 138 KLSWRFHTKYLTWFQRHEEPKQI---TDDYEQGTYVYFDFEKWSQRKKESFTFEYKFLED 194
Query: 584 EEFE 587
+EF+
Sbjct: 195 KEFD 198
>ECU06g1510 [K] KOG2150 CCR4-NOT transcriptional regulation complex NOT5
subunit
Length = 227
Score = 54.7 bits (130), Expect = 4e-07
Identities = 48/171 (28%), Positives = 74/171 (43%), Gaps = 29/171 (16%)
Query: 419 KKKLIQPPNEIDEFTLKHLE----SSLLNCPDSLDADTPRLFENPLSLPHPTSIFFPHEP 474
K++++Q N ++ + E +SL++ P+ D + + PL P FFP P
Sbjct: 72 KERILQHGNGHEDSVRRFYEVETSNSLVHIPNFRDLEKKYVPRTPLETPS----FFPKTP 127
Query: 475 IR-FMAQDLPAKGDDLYGKISVAKIMSKFALDTLFFIFYHYQGSYDQFLASRELSLRGWT 533
+ F +QD I K +DTLFFIFY G+ Q+ A+ +L W
Sbjct: 128 LYVFESQD----------------IFKKLDIDTLFFIFYSQLGTIQQYYAAVQLKTYSWR 171
Query: 534 FNKVNRCWFHKEVEKLPPGIEGK-EEVTWRYFDYQKAW-LSRRCGPDFEYK 582
F+ WF + E P I E + +FDY W ++ FEYK
Sbjct: 172 FHTKYFTWFQRLDE--PKLITADYERGDFLFFDYDVTWSFMKKSDFTFEYK 220
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.133 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,354,793
Number of Sequences: 60738
Number of extensions: 1379370
Number of successful extensions: 4910
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4872
Number of HSP's gapped (non-prelim): 16
length of query: 590
length of database: 30,389,216
effective HSP length: 112
effective length of query: 478
effective length of database: 23,586,560
effective search space: 11274375680
effective search space used: 11274375680
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)