ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2390 good U KOG4635 Intracellular trafficking, secretion, and vesicular transport Vacuolar import and degradation protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2390 834414 835412 333
(333 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR105c [U] KOG4635 Vacuolar import and degradation protein 214 2e-55
YGR066c [U] KOG4635 Vacuolar import and degradation protein 105 9e-23
HsM13129020 [U] KOG4635 Vacuolar import and degradation protein 97 3e-20
Hs21362076 [U] KOG4635 Vacuolar import and degradation protein 94 2e-19
SPAC3H1.14 [U] KOG4635 Vacuolar import and degradation protein 60 3e-09
ECU03g0570 [U] KOG4635 Vacuolar import and degradation protein 59 8e-09
>YBR105c [U] KOG4635 Vacuolar import and degradation protein
Length = 362
Score = 214 bits (544), Expect = 2e-55
Identities = 132/325 (40%), Positives = 183/325 (55%), Gaps = 43/325 (13%)
Query: 48 PIAFPAPLTPFFH---RNPYFRNTSLHRTGSHDSINSIRR-SHE-NFQDSEDYDIQDXXX 102
PI F T FH R+ Y + L++ S DS++S+++ +H N + Y ++D
Sbjct: 42 PITFNLTSTAPFHLHDRHRYLQEQDLYKCASRDSLSSLQQLAHTPNGSTRKKYIVEDQSP 101
Query: 103 XXXXXXXXXXXQTPKNHER-THWLGARKRFVGYQLSGYKKFQVMITIQSVSLPNDNESNC 161
NH T++L R +F GYQ+SGYK++QV + +++V LP +
Sbjct: 102 YSSENPVIVTSSY--NHTVCTNYLRPRMQFTGYQISGYKRYQVTVNLKTVDLPK-KDCTS 158
Query: 162 CSPHLTGFLSIKGLTVQNPEITTFFESITVTDS-LGFLS---KDIPIDYASYKASDQTDL 217
SPHL+GFLSI+GLT Q+PEI+T+FE+ V LGFLS KD P+ +KA+DQTDL
Sbjct: 159 LSPHLSGFLSIRGLTNQHPEISTYFEAYAVNHKELGFLSSSWKDEPV-LNEFKATDQTDL 217
Query: 218 EHWLNFPSFKELCMQND------------------------PKYPSKNI--LQSIIDGTY 251
EHW+NFPSF++L + + P PS + + I
Sbjct: 218 EHWINFPSFRQLFLMSQKNGLNSTDDNGTTNAAKKLPPQQLPTTPSADAGNISRIFSQEK 277
Query: 252 SHTDYLNQRFLYMRWNEKFLVPDADL-ESIDGASYDGYYYIVHDQLLGNILGFYYHEHAE 310
+YLN+RF++M+W EKFLVPDA L E +DGASYDG+YYIVHDQ+ GNI GFYYH+ AE
Sbjct: 278 QFDNYLNERFIFMKWKEKFLVPDALLMEGVDGASYDGFYYIVHDQVTGNIQGFYYHQDAE 337
Query: 311 RFXXXXXXXXXXXSI--GDCSFEFA 333
+F + DCSFEFA
Sbjct: 338 KFQQLELVPSLKNKVESSDCSFEFA 362
>YGR066c [U] KOG4635 Vacuolar import and degradation protein
Length = 292
Score = 105 bits (262), Expect = 9e-23
Identities = 62/196 (31%), Positives = 100/196 (50%), Gaps = 23/196 (11%)
Query: 130 RFVGYQLSGYKKFQVMITIQSVSLPNDNESNCCSPHLTGFLSIKGLTVQNPEITTFFESI 189
RF G Q S Y + V + I +V+LP +S PH+TG +IK LT ++ T FE
Sbjct: 84 RFGGSQSSKYTYYTVEVKIDTVNLPLYKDSRSLDPHVTGTFTIKNLTPVLDKVVTLFEGY 143
Query: 190 TVT-DSLGFLSKDIPIDYA--SYKASDQTDLEHWLNFPSFKELCMQNDPKYPSKNILQSI 246
+ + S P + Y A ++D HW F F ++ +N
Sbjct: 144 VINYNQFPLCSLHWPAEETLDPYMAQRESDCSHWKRFGHFGS----DNWSLTERNF---- 195
Query: 247 IDGTYSH--TDYLNQRFLYMRWNEKFLVPDADLESI--------DGASYDGYYYIVHDQL 296
G Y+H +++NQR++Y++W E+FL+ D + E++ +GAS++G+YY+ DQL
Sbjct: 196 --GQYNHESAEFMNQRYIYLKWKERFLLDDEEQENLMLDDNHHLEGASFEGFYYVCLDQL 253
Query: 297 LGNILGFYYHEHAERF 312
G++ G+YYH E F
Sbjct: 254 TGSVEGYYYHPACELF 269
>HsM13129020 [U] KOG4635 Vacuolar import and degradation protein
Length = 217
Score = 97.4 bits (241), Expect = 3e-20
Identities = 57/189 (30%), Positives = 92/189 (48%), Gaps = 28/189 (14%)
Query: 122 THWLGARKRFVGYQLSGYKKFQVMITIQSVSLPNDNESNCCSPHLTGFLSIKGLTVQNPE 181
T L + +F G+Q S + V + +Q V N +L G+L IKGLT + P
Sbjct: 36 TSLLYSGSKFRGHQKSKGNSYDVEVVLQHVDTGNS--------YLCGYLKIKGLTEEYPT 87
Query: 182 ITTFFESITVTDSLGFLSKDIPIDYASYKASDQTDLEHWLNFPSFKELCMQNDPKYPSKN 241
+TTFFE ++ FL++ + A + D +HW F +F +
Sbjct: 88 LTTFFEGEIISKKHPFLTR-------KWDADEDVDRKHWGKFLAFYQYA----------- 129
Query: 242 ILQSIIDGTYSHTDYLNQRFLYMRWNEKFLVPDADLESIDGASYDGYYYIVHDQLLGNIL 301
+S + + + N +++MRW E+FLVPD ++ I GAS+ G+YYI + +I
Sbjct: 130 --KSFNSDDFDYEELKNGDYVFMRWKEQFLVPDHTIKDISGASFAGFYYICFQKSAASIE 187
Query: 302 GFYYHEHAE 310
G+YYH +E
Sbjct: 188 GYYYHRSSE 196
>Hs21362076 [U] KOG4635 Vacuolar import and degradation protein
Length = 300
Score = 94.4 bits (233), Expect = 2e-19
Identities = 56/189 (29%), Positives = 91/189 (47%), Gaps = 28/189 (14%)
Query: 122 THWLGARKRFVGYQLSGYKKFQVMITIQSVSLPNDNESNCCSPHLTGFLSIKGLTVQNPE 181
T L + +F G+Q S + V + ++ V N +L G+L IKGLT + P
Sbjct: 119 TSLLYSGSKFRGHQKSKGNSYDVEVVLRHVDTGNS--------YLCGYLKIKGLTEEYPT 170
Query: 182 ITTFFESITVTDSLGFLSKDIPIDYASYKASDQTDLEHWLNFPSFKELCMQNDPKYPSKN 241
+TTFFE ++ FL++ + A + D +HW F +F +
Sbjct: 171 LTTFFEGEIISKKHPFLTR-------KWDADEDVDRKHWGKFLAFYQYA----------- 212
Query: 242 ILQSIIDGTYSHTDYLNQRFLYMRWNEKFLVPDADLESIDGASYDGYYYIVHDQLLGNIL 301
+S + + + N +++MRW E+FLVPD ++ I GAS+ G+YYI + +I
Sbjct: 213 --KSFNSDDFDYEELKNGDYVFMRWKEQFLVPDHTIKDISGASFAGFYYICFQKSAASIE 270
Query: 302 GFYYHEHAE 310
G YYH +E
Sbjct: 271 GHYYHRSSE 279
>SPAC3H1.14 [U] KOG4635 Vacuolar import and degradation protein
Length = 191
Score = 60.5 bits (145), Expect = 3e-09
Identities = 45/157 (28%), Positives = 70/157 (43%), Gaps = 25/157 (15%)
Query: 160 NCCSPHLTGFLSIKGLTVQNPEITTFFESITVTDSLGFLSKDIPIDYASYKASDQTDLEH 219
N L GF+ + + N +TT+FE+ + + F +K + + AS++ D H
Sbjct: 35 NLAKSMLCGFIHVSYTSPNNTSLTTYFEAEIIGNRFTFETK-----WPEWGASEEIDNRH 89
Query: 220 WLNFPSFKELCMQNDPKYPSKNILQSIIDGTYSHTDYLNQRFLYMRWNE-KFLVPDADLE 278
W + K N P + I D L++ +YMRW E L D +
Sbjct: 90 WKRLGALKS----NGKDIPLRLI---------QPYDPLSRETVYMRWKELAMLDKTVDYQ 136
Query: 279 SID-----GASYDGYYYIVHDQLLGNILGFYYHEHAE 310
+ + G SY+G+YYI Q G I G+YYH H+E
Sbjct: 137 NHNQSFPFGISYEGFYYISFSQSTGKIKGYYYH-HSE 172
>ECU03g0570 [U] KOG4635 Vacuolar import and degradation protein
Length = 200
Score = 59.3 bits (142), Expect = 8e-09
Identities = 47/179 (26%), Positives = 80/179 (44%), Gaps = 33/179 (18%)
Query: 130 RFVGYQLSGYKKFQVMITIQSVSLPNDNESNCCSPHLTGFLSIKGLTVQNPEITTFFESI 189
R+ G QL+ F++ + I V + + + G I LT + +TT+FE
Sbjct: 29 RYRGEQLTNGGSFKIEMNIDVVDIQQEL--------ICGTFEIHNLTDRYELLTTYFEGE 80
Query: 190 TVTDSLGFLSKDIPIDYASYKASDQTDLEHWLNFPSFKELCMQNDPKYPSKNILQSIIDG 249
+ + F +++ + D+ HW FP ++ +Y K G
Sbjct: 81 IIGNIYPFTTEEYSMT--------NPDIIHWKLFPEWRG-------EYVYK-------PG 118
Query: 250 TYSHTDYLNQRFLYMRWNEKFLVPDADLESIDGASYDGYYYIVHDQLLGNILGFYYHEH 308
+Y D F+Y++ E FL+PD L+SI GAS DG+YY + + L G YY+++
Sbjct: 119 SY---DIKKSNFMYIKIKELFLLPDPRLKSIPGASIDGHYYCCYYKNLDCFAGHYYYKN 174
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,839,868
Number of Sequences: 60738
Number of extensions: 760234
Number of successful extensions: 1516
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1501
Number of HSP's gapped (non-prelim): 6
length of query: 333
length of database: 30,389,216
effective HSP length: 107
effective length of query: 226
effective length of database: 23,890,250
effective search space: 5399196500
effective search space used: 5399196500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)