ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2466 good R KOG1947 General function prediction only Leucine rich repeat proteins, some proteins contain F-box
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2466 860436 859165 -424
(424 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR158w [R] KOG1947 Leucine rich repeat proteins some proteins c... 150 3e-36
7303582 [R] KOG4341 F-box protein containing LRR 71 3e-12
CE24780 [R] KOG4341 F-box protein containing LRR 62 1e-09
YJR090c [R] KOG1947 Leucine rich repeat proteins some proteins c... 58 3e-08
Hs16306582 [R] KOG4341 F-box protein containing LRR 52 1e-06
At5g23340 [R] KOG1947 Leucine rich repeat proteins some proteins... 52 1e-06
Hs14249620 [R] KOG4341 F-box protein containing LRR 50 5e-06
>YBR158w [R] KOG1947 Leucine rich repeat proteins some proteins contain
F-box
Length = 549
Score = 150 bits (380), Expect = 3e-36
Identities = 98/307 (31%), Positives = 157/307 (50%), Gaps = 15/307 (4%)
Query: 123 SLYRCSKVNPVWAAVASSIINKDLVIQQSSISGDLKIV----ELDQKVHPKSLTFYKLKQ 178
+L+ C VN +W V + K L + S+ + + E Q + P +KL Q
Sbjct: 240 ALFSCMMVNRLWLNVTRPFLFKSLHFK--SVHNFKEFLRTSQETTQVMRPSHFILHKLHQ 297
Query: 179 HRNFIENQLPRIRFTRLNSLNFYISP-VLPAI--FDDVTISNNLTKLTIAGNKKINDEEL 235
+L R+ L L FY+ P + P + FD++ + L KL I GNK I+D L
Sbjct: 298 VTQPDIERLSRMECQNLKWLEFYVCPRITPPLSWFDNL---HKLEKLIIPGNKNIDDNFL 354
Query: 236 ISLILGLPNLIDLDLRACXXXXXXXXXXXXTHCPKLQSINLGRHENSHLITDLSIMALSE 295
+ L +PNL L LRAC +CPKL++ N+GRH +LIT +S++AL +
Sbjct: 355 LRLSQSIPNLKHLVLRACDNVSDSGVVCIALNCPKLKTFNIGRHRRGNLITSVSLVALGK 414
Query: 296 LEHLTTVGFSGCDKISDVSIWQLYSKHSTTLVRLSINGCTQISDSSISDIVAKHGFPNLK 355
+ TVGF+GCD + D IW+ + + RLS+N C ++D S+ + A + FPNL
Sbjct: 415 YTQVETVGFAGCD-VDDAGIWEFARLNGKNVERLSLNSCRLLTDYSLPILFALNSFPNLA 473
Query: 356 VLDIRNC-QLVRFKNLIQYRDWRHKYLMKPIHIHISDSMKKEFELQEQQLYKEFRTRIVN 414
VL+IRN ++ ++ ++Y W+ K L PI I + + K + +E ++ + +
Sbjct: 474 VLEIRNLDKITDVRHFVKYNLWK-KSLDAPILIEACERITKLIDQEENRVKRINSLVALK 532
Query: 415 DLNRWVN 421
D+ WVN
Sbjct: 533 DMTAWVN 539
>7303582 [R] KOG4341 F-box protein containing LRR
Length = 464
Score = 71.2 bits (173), Expect = 3e-12
Identities = 80/314 (25%), Positives = 140/314 (44%), Gaps = 64/314 (20%)
Query: 110 ELLHRIFNFLDHESLYRCSKVNPVWAAVA---SSIINKDLVIQQSSISGD---------- 156
E+L R+F++LD SL RC++V W +A SS +L Q I G
Sbjct: 59 EVLLRVFSYLDVVSLCRCAQVCKYWNVLALDGSSWQKINLFDFQRDIEGPVIENISQRCR 118
Query: 157 --LKIVEL-------DQKV-----HPKSLTFYKLKQHRNF--IENQLPRIRFTRLNSLNF 200
LK + L DQ V H ++ L + I Q ++L ++N
Sbjct: 119 GFLKSLSLRGCQSVGDQSVRTLANHCHNIEHLDLSDCKKITDISTQSISRYCSKLTAINL 178
Query: 201 ------------YISPVLPAIFD-DVT----ISNN-----------LTKLTIAGNKKIND 232
Y+S P + + +V+ IS N L K + G K+IND
Sbjct: 179 HSCSNITDNSLKYLSDGCPNLMEINVSWCHLISENGVEALARGCVKLRKFSSKGCKQIND 238
Query: 233 EELISLILGLPNLIDLDLRACXXXXXXXXXXXXTHCPKLQSINLGRHENSHLITDLSIMA 292
++ L P+L+ L+L +C +C KLQ + + + + +TDL++++
Sbjct: 239 NAIMCLAKYCPDLMVLNLHSCETITDSSIRQLAANCHKLQKLCVSKCAD---LTDLTLLS 295
Query: 293 LSELEH-LTTVGFSGCDKISDVSIWQLYSKHSTTLVRLSINGCTQISDSSISDIVAKHGF 351
LS+ H L T+ SGC +D+ +Q ++ L R+ + C+QI+D +++ + G
Sbjct: 296 LSQHNHLLNTLEVSGCRNFTDIG-FQALGRNCKYLERMDLEECSQITDLTLAHLAT--GC 352
Query: 352 PNLKVLDIRNCQLV 365
P+L+ L + +C+L+
Sbjct: 353 PSLEKLTLSHCELI 366
>CE24780 [R] KOG4341 F-box protein containing LRR
Length = 461
Score = 62.4 bits (150), Expect = 1e-09
Identities = 50/173 (28%), Positives = 88/173 (49%), Gaps = 13/173 (7%)
Query: 212 DVTISN------NLTKLTIAGNKKINDEELISLILGLPNLIDLDLRACXXXXXXXXXXXX 265
D+T+ N L L ++ +I+D L+SL NL L+L C
Sbjct: 268 DITVQNIANGATALEYLCMSNCNQISDRSLVSLGQHSHNLKVLELSGCTLLGDNGFIPLA 327
Query: 266 THCPKLQSINLGRHENSHLITDLSIMALSE-LEHLTTVGFSGCDKISDVSIWQLYSKHST 324
C +L+ +++ E+ LI+D +I +L+ L + S C+ I+D SI L SKH
Sbjct: 328 RGCRQLERLDM---EDCSLISDHTINSLANNCTALRELSLSHCELITDESIQNLASKHRE 384
Query: 325 TLVRLSINGCTQISDSSISDIVAKHGFPNLKVLDIRNCQLVRFKNLIQYRDWR 377
TL L ++ C Q++DS++S + +H LK +D+ +CQ V + +++++ R
Sbjct: 385 TLNVLELDNCPQLTDSTLSHL--RH-CKALKRIDLYDCQNVSKEAIVRFQHHR 434
Score = 52.0 bits (123), Expect = 2e-06
Identities = 76/316 (24%), Positives = 133/316 (42%), Gaps = 53/316 (16%)
Query: 90 LLVSLSTS--DDSKSNPIFHIPELLHRIFNFLDHESLYRCSKVNPVWAAVA---SSIINK 144
L+ +LS + D+S N + E+L ++F+FLD ++L R ++V W+ +A S+
Sbjct: 42 LITTLSPAQVDNSLINRVLP-KEVLLKVFSFLDTKALCRSAQVCRSWSILALDGSNWQRV 100
Query: 145 DLVIQQSSI------------SGDLKIVELD--QKVHPKS-------------LTFYKLK 177
DL Q + G LK + L + VH + L+ Y+ K
Sbjct: 101 DLFTFQRDVKTAVVENLARRCGGFLKELSLKGCENVHDSALRTFTSRCPNLEHLSLYRCK 160
Query: 178 QHRNFIENQLPR----IRFTRLNSLNFYISPVLPAIFDDVTISNNLTKLTIAGNKKINDE 233
+ + L R + + L + + + I D NL+ L I+ I D
Sbjct: 161 RVTDASCENLGRYCHKLNYLNLENCSSITDRAMKYIGDGCP---NLSYLNISWCDAIQDR 217
Query: 234 ELISLILGLPNLIDLDLRACXXXXXXXXXXXXTHCPKLQSINLGRHENSHLITDLSIMAL 293
+ ++ +L L LR C H ++ +NL +TD+++ +
Sbjct: 218 GVQIILSNCKSLDTLILRGCEGLTENVFGSVEAHMGAIKKLNL---LQCFQLTDITVQNI 274
Query: 294 SE----LEHLTTVGFSGCDKISDVSIWQLYSKHSTTLVRLSINGCTQISDSSISDIVAKH 349
+ LE+L S C++ISD S+ L +HS L L ++GCT + D+ I
Sbjct: 275 ANGATALEYLC---MSNCNQISDRSLVSL-GQHSHNLKVLELSGCTLLGDNGF--IPLAR 328
Query: 350 GFPNLKVLDIRNCQLV 365
G L+ LD+ +C L+
Sbjct: 329 GCRQLERLDMEDCSLI 344
>YJR090c [R] KOG1947 Leucine rich repeat proteins some proteins contain
F-box
Length = 1151
Score = 57.8 bits (138), Expect = 3e-08
Identities = 61/230 (26%), Positives = 108/230 (46%), Gaps = 38/230 (16%)
Query: 169 KSLTFYKLKQHRNFIENQ--LPRIRFTRLNSLNFYISPVL----PAIFD-DVTISNNLT- 220
+++TF L RNFI + L RI+ T N++N + +L P + + D+T+S N+T
Sbjct: 477 RNVTFDSL---RNFIVHSPMLKRIKITANNNMNDELVELLANKCPLLVEVDITLSPNVTD 533
Query: 221 --------------KLTIAGNKKIND---EELISLILGLPNLIDLDLRACXXXXXXXXXX 263
+ I N I D +EL ++ +P+L +DL C
Sbjct: 534 SSLLKLLTRLVQLREFRITHNTNITDNLFQELSKVVDDMPSLRLIDLSGCENITDKTIES 593
Query: 264 XXTHCPKLQSINLGRHENSHLITDLSIMALSEL-EHLTTVGFSGCDKISDVSIWQLYSKH 322
PKL+++ LG+ ITD S+ LS+L ++L TV F C I+D + L+ H
Sbjct: 594 IVNLAPKLRNVFLGKCSR---ITDASLFQLSKLGKNLQTVHFGHCFNITDNGVRALF--H 648
Query: 323 STTLVR-LSINGCTQISDSSISDIVAKHGFPNLKVLDIRNCQLVRFKNLI 371
S T ++ + CT +++ ++ ++ P LK + + C + + L+
Sbjct: 649 SCTRIQYVDFACCTNLTNRTLYELA---DLPKLKRIGLVKCTQMTDEGLL 695
>Hs16306582 [R] KOG4341 F-box protein containing LRR
Length = 423
Score = 52.4 bits (124), Expect = 1e-06
Identities = 44/151 (29%), Positives = 67/151 (44%), Gaps = 6/151 (3%)
Query: 217 NNLTKLTIAGNKKINDEELISLILGLPNLIDLDLRACXXXXXXXXXXXXTHCPKLQSINL 276
+ L L + +I DE ++ + G L L L C +CP+LQ +
Sbjct: 208 HELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEA 267
Query: 277 GRHENSHLITDLSIMALSELEH-LTTVGFSGCDKISDVSIWQLYSKHSTTLVRLSINGCT 335
R SHL TD L+ H L + C I+D ++ QL S H L LS++ C
Sbjct: 268 AR--CSHL-TDAGFTLLARNCHELEKMDLEECILITDSTLIQL-SIHCPKLQALSLSHCE 323
Query: 336 QISDSSISDIV-AKHGFPNLKVLDIRNCQLV 365
I+D I + + G L+VL++ NC L+
Sbjct: 324 LITDDGILHLSNSTCGHERLRVLELDNCLLI 354
>At5g23340 [R] KOG1947 Leucine rich repeat proteins some proteins contain
F-box
Length = 405
Score = 52.4 bits (124), Expect = 1e-06
Identities = 43/149 (28%), Positives = 68/149 (44%), Gaps = 5/149 (3%)
Query: 218 NLTKLTIAGNKKINDEELISLILGLPNLIDLDLRACXXXXXXXXXXXXTHCPKLQSINLG 277
+L L + G I D L L+ G + LD+ C C S+
Sbjct: 177 DLEALGLQGCTNITDSGLADLVKGCRKIKSLDINKCSNVGDAGVSSVAKACAS--SLKTL 234
Query: 278 RHENSHLITDLSIMALSEL-EHLTTVGFSGCDKISDVSIWQLYSKHSTTLVRLSINGCTQ 336
+ + + + + SI +L++ ++L T+ GC ISD SI L +L L ++ C
Sbjct: 235 KLLDCYKVGNESISSLAQFCKNLETLIIGGCRDISDESIMLLADSCKDSLKNLRMDWCLN 294
Query: 337 ISDSSISDIVAKHGFPNLKVLDIRNCQLV 365
ISDSS+S I+ + NL+ LDI C+ V
Sbjct: 295 ISDSSLSCILKQ--CKNLEALDIGCCEEV 321
>Hs14249620 [R] KOG4341 F-box protein containing LRR
Length = 422
Score = 50.4 bits (119), Expect = 5e-06
Identities = 40/148 (27%), Positives = 69/148 (46%), Gaps = 7/148 (4%)
Query: 219 LTKLTIAGNKKINDEELISLILGLPNLIDLDLRACXXXXXXXXXXXXTHCPKLQSINLGR 278
L L + G ++ DE L + P L+ L+L+ C C KLQS+
Sbjct: 183 LKALFLKGCTQLEDEALKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASG 242
Query: 279 HENSHLITDLSIMALSE-LEHLTTVGFSGCDKISDVSIWQLYSKHSTTLVRLSINGCTQI 337
N ITD + AL + L + + C +++DV L +++ L ++ + C QI
Sbjct: 243 CSN---ITDAILNALGQNCPRLRILEVARCSQLTDVGFTTL-ARNCHELEKMDLEECVQI 298
Query: 338 SDSSISDIVAKHGFPNLKVLDIRNCQLV 365
+DS++ I P L+VL + +C+L+
Sbjct: 299 TDSTL--IQLSIHCPRLQVLSLSHCELI 324
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,353,875
Number of Sequences: 60738
Number of extensions: 1012788
Number of successful extensions: 2980
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2934
Number of HSP's gapped (non-prelim): 25
length of query: 424
length of database: 30,389,216
effective HSP length: 109
effective length of query: 315
effective length of database: 23,768,774
effective search space: 7487163810
effective search space used: 7487163810
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)