ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2475 suspect: HSP O KOG1039 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2475 862234 863526 431
(431 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL054w [O] KOG1039 Predicted E3 ubiquitin ligase 121 2e-27
SPAC3A11.02 [O] KOG1039 Predicted E3 ubiquitin ligase 92 1e-18
SPCC1739.01_1 [O] KOG1039 Predicted E3 ubiquitin ligase 81 3e-15
Hs8850223 [O] KOG1039 Predicted E3 ubiquitin ligase 63 1e-09
Hs7305273 [O] KOG1039 Predicted E3 ubiquitin ligase 63 1e-09
Hs14730765 [O] KOG1039 Predicted E3 ubiquitin ligase 63 1e-09
Hs13540509 [O] KOG1039 Predicted E3 ubiquitin ligase 59 2e-08
Hs22041107 [A] KOG1040 Polyadenylation factor I complex subunit ... 52 2e-06
Hs14210506 [A] KOG1040 Polyadenylation factor I complex subunit ... 52 2e-06
Hs22050713 [A] KOG1040 Polyadenylation factor I complex subunit ... 50 7e-06
>YPL054w [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 301
Score = 121 bits (303), Expect = 2e-27
Identities = 60/105 (57%), Positives = 74/105 (70%), Gaps = 6/105 (5%)
Query: 49 NSGINIE--CASSEQ-GFTPQQQKAIIQHLLITKN-SAPKKDYSHVPCKFFVQGNCQAGD 104
N+ +N+ C Q F+ +QQK I++HLLITKN S +KDYSHVPCKFF GNCQAG
Sbjct: 46 NANVNLSKNCTVGNQLPFSSRQQKIIMEHLLITKNNSQQQKDYSHVPCKFFKMGNCQAGS 105
Query: 105 SCPFSH--DLNNSTSEQVCKYFQKGNCKFGMKCANAHISSNGTRV 147
SCPFSH D+ +S + CKYF KGNCKFG KC NAH+ NG ++
Sbjct: 106 SCPFSHSPDIISSANNLPCKYFAKGNCKFGNKCVNAHVLPNGFKM 150
>SPAC3A11.02 [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 583
Score = 92.0 bits (227), Expect = 1e-18
Identities = 41/93 (44%), Positives = 56/93 (60%), Gaps = 1/93 (1%)
Query: 85 KDYSHVPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAHISSNG 144
K HVPCKFF QG C +G +C FSHDL +T + +CKYFQKGNCKFG KCA H+ +G
Sbjct: 34 KSLQHVPCKFFRQGTCTSGKNCIFSHDLELATEKTICKYFQKGNCKFGSKCALEHVLPDG 93
Query: 145 TRVXXXXXXXXXXXXGKNNFTLTTR-VPDVISN 176
+V G ++ ++ + ++ISN
Sbjct: 94 RKVKTRAFAPSTTAMGSSSQNISAAPMANIISN 126
>SPCC1739.01_1 [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 319
Score = 80.9 bits (198), Expect = 3e-15
Identities = 35/63 (55%), Positives = 41/63 (64%), Gaps = 2/63 (3%)
Query: 85 KDYSHVPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAHISSNG 144
K+ HVPCKFF G C AG++CPFSH L T +CKYF KGNCKFG KCA +H
Sbjct: 40 KNLQHVPCKFFRNGTCTAGENCPFSHSL--ETERPICKYFLKGNCKFGPKCALSHALPGN 97
Query: 145 TRV 147
T +
Sbjct: 98 TNL 100
>Hs8850223 [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 416
Score = 62.8 bits (151), Expect = 1e-09
Identities = 23/50 (46%), Positives = 32/50 (64%)
Query: 90 VPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAH 139
+ C++F+ G C+ G C FSHDL NS +CKY+QKG C +G +C H
Sbjct: 6 ITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGTRCRYDH 55
>Hs7305273 [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 482
Score = 62.8 bits (151), Expect = 1e-09
Identities = 24/50 (48%), Positives = 35/50 (70%)
Query: 90 VPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAH 139
V C++F+ G C+ GD+C +SHDL++S VCKYFQ+G C +G +C H
Sbjct: 59 VTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEH 108
>Hs14730765 [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 416
Score = 62.8 bits (151), Expect = 1e-09
Identities = 23/50 (46%), Positives = 32/50 (64%)
Query: 90 VPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAH 139
+ C++F+ G C+ G C FSHDL NS +CKY+QKG C +G +C H
Sbjct: 6 ITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGTRCRYDH 55
>Hs13540509 [O] KOG1039 Predicted E3 ubiquitin ligase
Length = 485
Score = 58.5 bits (140), Expect = 2e-08
Identities = 23/50 (46%), Positives = 33/50 (66%)
Query: 90 VPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAH 139
V C++F G C+ GD+C +SHDL++ VCKYFQ+G C +G +C H
Sbjct: 94 VTCRYFKYGICKEGDNCRYSHDLSDRLCGVVCKYFQRGCCVYGDRCRCEH 143
>Hs22041107 [A] KOG1040 Polyadenylation factor I complex subunit Yth1 (CPSF
subunit)
Length = 1048
Score = 51.6 bits (122), Expect = 2e-06
Identities = 18/48 (37%), Positives = 27/48 (55%)
Query: 92 CKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAH 139
CK+F++G C GD C F HD +++CK++ +G C G C H
Sbjct: 80 CKYFLEGRCIKGDQCKFDHDAELEKRKEICKFYLQGYCTKGENCIYMH 127
>Hs14210506 [A] KOG1040 Polyadenylation factor I complex subunit Yth1 (CPSF
subunit)
Length = 291
Score = 51.6 bits (122), Expect = 2e-06
Identities = 22/82 (26%), Positives = 41/82 (49%), Gaps = 2/82 (2%)
Query: 58 SSEQGFTPQQQKAIIQHLLITKNSAPKKDYSHVPCKFFVQGNCQAGDSCPFSHDLNNSTS 117
S E+ P++++ + I +++ +K CK+F++ C GD C F HD
Sbjct: 165 SQEEDGKPKEKQQHLSQAFINQHTVERKGKQI--CKYFLERKCIKGDQCKFDHDAEIEKK 222
Query: 118 EQVCKYFQKGNCKFGMKCANAH 139
+++CK++ +G C G C H
Sbjct: 223 KEMCKFYVQGFCSRGENCLYLH 244
>Hs22050713 [A] KOG1040 Polyadenylation factor I complex subunit Yth1 (CPSF
subunit)
Length = 1303
Score = 50.1 bits (118), Expect = 7e-06
Identities = 20/50 (40%), Positives = 27/50 (54%)
Query: 90 VPCKFFVQGNCQAGDSCPFSHDLNNSTSEQVCKYFQKGNCKFGMKCANAH 139
V CK+FV+G C GD C FSHD+ ++CK++ G C C H
Sbjct: 394 VICKYFVEGRCTWGDHCNFSHDIELPKKRELCKFYITGFCARAENCPYMH 443
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.131 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,306,377
Number of Sequences: 60738
Number of extensions: 751048
Number of successful extensions: 1613
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1567
Number of HSP's gapped (non-prelim): 33
length of query: 431
length of database: 30,389,216
effective HSP length: 109
effective length of query: 322
effective length of database: 23,768,774
effective search space: 7653545228
effective search space used: 7653545228
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)