ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactII2531   good          A     KOG1869  RNA processing and modification                                Splicing coactivator SRm160/300, subunit SRm300

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactII2531 880175  880627 151  
         (151 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR482c [A] KOG1869 Splicing coactivator SRm160/300 subunit SRm300     79   2e-15
CE25503 [A] KOG1869 Splicing coactivator SRm160/300 subunit SRm300     61   5e-10
At3g49600_1 [A] KOG1869 Splicing coactivator SRm160/300 subunit ...    54   1e-07
Hs19923466 [A] KOG1869 Splicing coactivator SRm160/300 subunit S...    49   3e-06

>YDR482c [A] KOG1869 Splicing coactivator SRm160/300 subunit SRm300
          Length = 135

 Score = 79.0 bits (193), Expect = 2e-15
 Identities = 50/116 (43%), Positives = 66/116 (56%), Gaps = 7/116 (6%)

Query: 39  MSYNGIGLSSAKGSSTSGYVQQSLAFTNRKK-----DPRLTTHDKEXXXXXXXXXXXPAK 93
           MSYNGIGL SAKGSSTSG+VQ+SLA  NR++       R    +              A 
Sbjct: 1   MSYNGIGLKSAKGSSTSGHVQRSLASNNRRRPQGSQQQRQQRQNAIKKASHDKASRPLAV 60

Query: 94  DESVISHKAKRQIELLVSEYRDKLEDGPDDLSDDTIDSKCEDYRRSIL-EGNEQSR 148
            + + +H  KR+IE+ VSE RD+LE+  + LS++ ID KCE  R  +  E  EQ R
Sbjct: 61  QKQIETHMEKREIEVQVSELRDRLEE-EETLSEEQIDKKCEALRAKLTNEWQEQQR 115


>CE25503 [A] KOG1869 Splicing coactivator SRm160/300 subunit SRm300
          Length = 425

 Score = 61.2 bits (147), Expect = 5e-10
 Identities = 35/102 (34%), Positives = 57/102 (55%), Gaps = 4/102 (3%)

Query: 41  YNGIGLSSAKGSSTSGYVQQSLAFTNRKKDPRLTTHDKEXXXXXXXXXXXPAKDESVISH 100
           YNGIGL +A+GS T+GYVQ +L+   + +  R   ++ E              +E ++ H
Sbjct: 2   YNGIGLQTARGSGTNGYVQSNLSHLMQAR--RKIEYNGEDDLRKMEAELNRKPNEEIMDH 59

Query: 101 KAKRQIELLVSEYRDKLEDGPDDLSDDTIDSKCEDYRRSILE 142
             KRQIE+  +E+   LED    L D+ I+ K  +YR+++L+
Sbjct: 60  NRKRQIEVKCTEFEMLLED--KGLDDEDIERKVGEYRKNLLK 99


>At3g49600_1 [A] KOG1869 Splicing coactivator SRm160/300 subunit SRm300
          Length = 637

 Score = 53.5 bits (127), Expect = 1e-07
 Identities = 33/100 (33%), Positives = 48/100 (48%), Gaps = 2/100 (2%)

Query: 41  YNGIGLSSAKGSSTSGYVQQSLAFTNRKKDPRLTTHDKEXXXXXXXXXXXPAKDESVISH 100
           YNGIGL +A+GS T+GYVQ +  F   +   +     K               +++++ H
Sbjct: 2   YNGIGLQTARGSGTNGYVQTNKFFVRPRNGGKPVKGGKGFEDDEGTAGLSKKPNKAILEH 61

Query: 101 KAKRQIELLVSEYRDKLEDGPDDLSDDTIDSKCEDYRRSI 140
             KRQI L ++   DKL D     SD  I  K E+ R S+
Sbjct: 62  DRKRQIHLKLAILEDKLAD--QGYSDIEIAQKLEEARVSL 99


>Hs19923466 [A] KOG1869 Splicing coactivator SRm160/300 subunit SRm300
          Length = 2752

 Score = 48.9 bits (115), Expect = 3e-06
 Identities = 28/102 (27%), Positives = 49/102 (47%), Gaps = 3/102 (2%)

Query: 41  YNGIGLSSAKGSSTSGYVQQSLAFTNRKKDPRLTTHDKEXXXXXXXXXXXPAKDESVISH 100
           YNGIGL + +GS T+GYVQ++L+    ++  R   +  E              +  ++ H
Sbjct: 2   YNGIGLPTPRGSGTNGYVQRNLSLVRGRRGER-PDYKGEEELRRLEAALVKRPNPDILDH 60

Query: 101 KAKRQIELLVSEYRDKLEDGPDDLSDDTIDSKCEDYRRSILE 142
           + KR++EL   E  + +E+      +  I  K   +R  +LE
Sbjct: 61  ERKRRVELRCLELEEMMEE--QGYEEQQIQEKVATFRLMLLE 100


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.131    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,887,253
Number of Sequences: 60738
Number of extensions: 320085
Number of successful extensions: 702
Number of sequences better than 1.0e-05: 4
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 696
Number of HSP's gapped (non-prelim): 4
length of query: 151
length of database: 30,389,216
effective HSP length: 97
effective length of query: 54
effective length of database: 24,497,630
effective search space: 1322872020
effective search space used: 1322872020
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)