ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2720 good E KOG0189 Amino acid transport and metabolism Phosphoadenosine phosphosulfate reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2720 940469 939678 -264
(264 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR167c [E] KOG0189 Phosphoadenosine phosphosulfate reductase 394 e-110
SPAC13G7.06 [E] KOG0189 Phosphoadenosine phosphosulfate reductase 296 2e-80
At1g62180_1 [E] KOG0189 Phosphoadenosine phosphosulfate reductase 82 8e-16
At4g21990_1 [E] KOG0189 Phosphoadenosine phosphosulfate reductase 79 9e-15
At4g04610_1 [E] KOG0189 Phosphoadenosine phosphosulfate reductase 76 4e-14
7298064 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfo... 57 3e-08
7292816 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfo... 51 2e-06
>YPR167c [E] KOG0189 Phosphoadenosine phosphosulfate reductase
Length = 261
Score = 394 bits (1013), Expect = e-110
Identities = 185/260 (71%), Positives = 218/260 (83%), Gaps = 3/260 (1%)
Query: 4 QVFQLNNAVTVTQEEIDHWNEKLSEL-SPEEIIRWAIVTFPHLFQTTAFGLTGLATIDML 62
+ + LNN + VTQE++DHWNE+L +L +P+EII W+IVTFPHLFQTTAFGLTGL TIDML
Sbjct: 2 KTYHLNNDIIVTQEQLDHWNEQLIKLETPQEIIAWSIVTFPHLFQTTAFGLTGLVTIDML 61
Query: 63 SKINQNETEPIVPLIFIDTLHHFPQTLELLKVVETRYYKPRGQNVNVYQPKDCGSEQEFA 122
SK+++ P L+FIDTLHHFPQTL L +E +YY+P+ Q ++VY+P C SE +FA
Sbjct: 62 SKLSEKYYMP--ELLFIDTLHHFPQTLTLKNEIEKKYYQPKNQTIHVYKPDGCESEADFA 119
Query: 123 AKYGDFLWDTDDDKYDFLVKVEPAHRAYKELQVTAVFTGRRKSQGGARSNLKFIELDEFN 182
+KYGDFLW+ DDDKYD+L KVEPAHRAYKEL ++AVFTGRRKSQG ARS L IE+DE N
Sbjct: 120 SKYGDFLWEKDDDKYDYLAKVEPAHRAYKELHISAVFTGRRKSQGSARSQLSIIEIDELN 179
Query: 183 HIIKINPLANWDFNQVQSYIRDNNVPYNELLDLGYRSIGDYHSTQPVQEGEDERSGRWKG 242
I+KINPL NW F QV+ YI NNVPYNELLDLGYRSIGDYHSTQPV+EGEDER+GRWKG
Sbjct: 180 GILKINPLINWTFEQVKQYIDANNVPYNELLDLGYRSIGDYHSTQPVKEGEDERAGRWKG 239
Query: 243 KAKTECGIHETSRFAQYLKQ 262
KAKTECGIHE SRFAQ+LKQ
Sbjct: 240 KAKTECGIHEASRFAQFLKQ 259
>SPAC13G7.06 [E] KOG0189 Phosphoadenosine phosphosulfate reductase
Length = 266
Score = 296 bits (758), Expect = 2e-80
Identities = 143/248 (57%), Positives = 193/248 (77%), Gaps = 12/248 (4%)
Query: 17 EEIDHWNEKLSELSPEEIIRWAIVTFPHLFQTTAFGLTGLATIDMLSKINQNETEPIVPL 76
E +++ N++LSELSP++I++W T P LFQT+A GL+GL +DMLSK++ N VPL
Sbjct: 20 EHLEYINKQLSELSPQDILKWCRWTLPSLFQTSALGLSGLVIMDMLSKMDMN-----VPL 74
Query: 77 IFIDTLHHFPQTLELLKVVETRYYKPRGQNVNVYQPKDCGSEQEFAAKYGDFLWDTDDDK 136
IFI+TLHHFP+TL+LL+ V+T+Y V+VY+ + +E+EFA K+G+ LW+TD+ +
Sbjct: 75 IFINTLHHFPETLDLLEKVKTKY---PNVPVHVYRCAEAANEKEFAQKFGEKLWETDESR 131
Query: 137 YDFLVKVEPAHRAYKELQVTAVFTGRRKSQGGARSNLKFIELDEFNHIIKINPLANWDFN 196
YDFLVKVEPA RAY +L V AVFTGRR+SQGG R +L ++LD ++KINPLANW F
Sbjct: 132 YDFLVKVEPASRAYSDLNVLAVFTGRRRSQGGERGSLPIVQLD--GPVLKINPLANWSFT 189
Query: 197 QVQSYIRDNNVPYNELLDLGYRSIGDYHSTQPVQEGEDERSGRWKGKAKTECGIHE--TS 254
+V +YI NNVPYNELL+ GYRS+GD+HSTQPV+EGEDER+GRW+G+ KTECG+H S
Sbjct: 190 EVHNYIITNNVPYNELLNKGYRSVGDWHSTQPVREGEDERAGRWRGREKTECGLHSHPQS 249
Query: 255 RFAQYLKQ 262
+FAQY+ +
Sbjct: 250 KFAQYMAE 257
>At1g62180_1 [E] KOG0189 Phosphoadenosine phosphosulfate reductase
Length = 320
Score = 82.0 bits (201), Expect = 8e-16
Identities = 79/249 (31%), Positives = 111/249 (43%), Gaps = 26/249 (10%)
Query: 17 EEIDHWNEKLSELSPEEIIRWAIVTFPHLFQTTAFGLTGLATIDMLSKINQNETEPIVPL 76
E+ + +KL + SP EI+ A+ F G +A I+ T +
Sbjct: 83 EDFEQLAKKLEDASPLEIMDKALERFGDQIAIAFSGAEDVALIEYARL-----TGKPFRV 137
Query: 77 IFIDTLHHFPQTLELLKVVETRYYKPRGQNVNVYQPKDCGSEQEFAAKYGDFLWDTDDDK 136
+DT P+T L VE +Y G + P D Q G F + D +
Sbjct: 138 FSLDTGRLNPETYRLFDAVEKQY----GIRIEYMFP-DAVEVQALVRNKGLFSFYEDGHQ 192
Query: 137 YDFLV-KVEPAHRAYKELQVTAVFTGRRKSQG-GARSNLKFIELDEF--------NHIIK 186
V KV P RA K L+ A TG+RK Q G RS + +++D ++K
Sbjct: 193 ECCRVRKVRPLRRALKGLK--AWITGQRKDQSPGTRSEIPIVQVDPVFEGLDGGVGSLVK 250
Query: 187 INPLANWDFNQVQSYIRDNNVPYNELLDLGYRSIGDYHSTQPVQEGEDERSGRW---KGK 243
NPLAN + V +++R +VP N L GY SIG T+PV G+ ER GRW K
Sbjct: 251 WNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPVLPGQHEREGRWWWEDAK 310
Query: 244 AKTECGIHE 252
AK ECG+H+
Sbjct: 311 AK-ECGLHK 318
>At4g21990_1 [E] KOG0189 Phosphoadenosine phosphosulfate reductase
Length = 341
Score = 78.6 bits (192), Expect = 9e-15
Identities = 77/252 (30%), Positives = 112/252 (43%), Gaps = 26/252 (10%)
Query: 14 VTQEEIDHWNEKLSELSPEEIIRWAIVTFPHLFQTTAFGLTGLATIDMLSKINQNETEPI 73
V E+ + ++L SP EI+ A+ F + G +A I+ + T
Sbjct: 80 VEVEDFEELAKRLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYA-----HLTGRP 134
Query: 74 VPLIFIDTLHHFPQTLELLKVVETRYYKPRGQNVNVYQPKDCGSEQEFAAKYGDFLWDTD 133
+ +DT P+T L VE Y G + P D Q G F + D
Sbjct: 135 YRVFSLDTGRLNPETYRLFDTVEKHY----GIRIEYMFP-DAVEVQALVRNKGLFSFYED 189
Query: 134 DDKYDFLV-KVEPAHRAYKELQVTAVFTGRRKSQG-GARSNLKFIELDEF--------NH 183
+ + KV P RA K L+ A TG+RK Q G RS + +++D
Sbjct: 190 GHQECCRIRKVRPLRRALKGLR--AWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 247
Query: 184 IIKINPLANWDFNQVQSYIRDNNVPYNELLDLGYRSIGDYHSTQPVQEGEDERSGRW--- 240
++K NP+AN + N V +++R +VP N L GY SIG T+ V G+ ER GRW
Sbjct: 248 LVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVLPGQHEREGRWWWE 307
Query: 241 KGKAKTECGIHE 252
KAK ECG+H+
Sbjct: 308 DAKAK-ECGLHK 318
>At4g04610_1 [E] KOG0189 Phosphoadenosine phosphosulfate reductase
Length = 328
Score = 76.3 bits (186), Expect = 4e-14
Identities = 76/249 (30%), Positives = 110/249 (43%), Gaps = 26/249 (10%)
Query: 17 EEIDHWNEKLSELSPEEIIRWAIVTFPHLFQTTAFGLTGLATIDMLSKINQNETEPIVPL 76
E+ + +KL SP EI+ A+ + + G +A I+ + T +
Sbjct: 91 EDFEELAKKLENASPLEIMDKALEKYGNDIAIAFSGAEDVALIEYA-----HLTGRPFRV 145
Query: 77 IFIDTLHHFPQTLELLKVVETRYYKPRGQNVNVYQPKDCGSEQEFAAKYGDFLWDTDDDK 136
+DT P+T VE Y G + P D Q G F + D +
Sbjct: 146 FSLDTGRLNPETYRFFDAVEKHY----GIRIEYMFP-DSVEVQGLVRSKGLFSFYEDGHQ 200
Query: 137 YDFLV-KVEPAHRAYKELQVTAVFTGRRKSQG-GARSNLKFIELDEF--------NHIIK 186
V KV P RA K L+ A TG+RK Q G RS + +++D ++K
Sbjct: 201 ECCRVRKVRPLRRALKGLK--AWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGSLVK 258
Query: 187 INPLANWDFNQVQSYIRDNNVPYNELLDLGYRSIGDYHSTQPVQEGEDERSGRW---KGK 243
NP+AN + N V +++R +VP N L GY SIG T+ V G+ ER GRW K
Sbjct: 259 WNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCTKAVLPGQHEREGRWWWEDAK 318
Query: 244 AKTECGIHE 252
AK ECG+H+
Sbjct: 319 AK-ECGLHK 326
>7298064 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 254
Score = 57.0 bits (136), Expect = 3e-08
Identities = 32/96 (33%), Positives = 54/96 (55%), Gaps = 7/96 (7%)
Query: 136 KYDFLVKVEPAHRAYKELQVTAVFTGRRKSQGGARSNLKFIELDEFNH----IIKINPLA 191
+Y+ ++KV ++ QV A+F G R+S + EL+ N+ +++I PL
Sbjct: 116 RYEGVLKVAFEQLIAEDSQVKAIFLGCRRSDP---ESCNLYELEPTNNGWPAMMRIFPLL 172
Query: 192 NWDFNQVQSYIRDNNVPYNELLDLGYRSIGDYHSTQ 227
W ++ + +Y+R NN+PY L D GY S+GD ST+
Sbjct: 173 EWSYHDIWNYLRSNNLPYCCLYDQGYTSLGDRSSTR 208
>7292816 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 294
Score = 50.8 bits (120), Expect = 2e-06
Identities = 29/94 (30%), Positives = 48/94 (50%), Gaps = 1/94 (1%)
Query: 136 KYDFLVKVEPAHRAYKELQVTAVFTGRRKSQGGARSNLKFIELD-EFNHIIKINPLANWD 194
+Y+ +K H + ++ AVF G R + + D ++ ++++NPL W
Sbjct: 153 QYEGTLKEALTHMSSDMPRIKAVFVGSRNTDPYCQHLAPMQPTDNDWPPMMRLNPLLEWS 212
Query: 195 FNQVQSYIRDNNVPYNELLDLGYRSIGDYHSTQP 228
++ V YI N+VPY L D GY SIG+ +T P
Sbjct: 213 YHDVWHYIHLNSVPYCSLYDRGYTSIGNRANTVP 246
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,743,226
Number of Sequences: 60738
Number of extensions: 736772
Number of successful extensions: 1748
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1737
Number of HSP's gapped (non-prelim): 7
length of query: 264
length of database: 30,389,216
effective HSP length: 104
effective length of query: 160
effective length of database: 24,072,464
effective search space: 3851594240
effective search space used: 3851594240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)