ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2757 good E KOG0571 Amino acid transport and metabolism Asparagine synthase (glutamine-hydrolyzing)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2757 952835 951114 -574
(574 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR145w [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 1001 0.0
YGR124w [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 994 0.0
SPBC119.10 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 748 0.0
At5g65010 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 550 e-156
At5g10240 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 546 e-155
At3g47340 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 541 e-153
CE04760 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 419 e-117
Hs19718772 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 249 8e-66
CE09642 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 243 4e-64
Hs20539333 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 125 2e-28
>YPR145w [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 572
Score = 1001 bits (2589), Expect = 0.0
Identities = 476/572 (83%), Positives = 514/572 (89%)
Query: 1 MCGIFASFRNPDVQAYKPKALQLSKRIRHRGPDWSGNIVKNSTILVHERLAIVGLDSGAQ 60
MCGIFA+FR+ DV YKPKALQLSKRIRHRGPDWSGN +KNSTI VHERLAIVG++SGAQ
Sbjct: 1 MCGIFAAFRHEDVHRYKPKALQLSKRIRHRGPDWSGNAIKNSTIFVHERLAIVGVESGAQ 60
Query: 61 PITSEDGSYTLAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDIDAPKHLDGMF 120
PITS DG Y L VNGEIYNHI LREECA Y F TLSDCEPIIP+Y K+DIDAPK+LDGMF
Sbjct: 61 PITSSDGEYMLCVNGEIYNHIQLREECADYEFGTLSDCEPIIPMYLKHDIDAPKYLDGMF 120
Query: 121 AWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIAFPPGHV 180
AW LYD+K+DRI+AARDPIGITTLYMGRSSASPK+V+F+SELKCLTDDCD I AFPPGHV
Sbjct: 121 AWTLYDAKQDRIVAARDPIGITTLYMGRSSASPKTVYFASELKCLTDDCDTITAFPPGHV 180
Query: 181 YDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVPYXXXXXXXXXX 240
YDS+TDK+TRYFTPDWLDE RIP+TP+D+ A+R SLEKAVRKRLMAEVPY
Sbjct: 181 YDSKTDKITRYFTPDWLDEKRIPSTPIDYMAIRHSLEKAVRKRLMAEVPYGVLLSGGLDS 240
Query: 241 XXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGWSRLHSFAVGLPNAPDL 300
IA+IAARET K +++P YDS LAG+D+ G LH AGW+ LHSFA+GLPNAPDL
Sbjct: 241 SLIASIAARETAKATNDVEPSTYDSKARHLAGIDDDGKLHTAGWTSLHSFAIGLPNAPDL 300
Query: 301 QAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQG 360
QAARKVA FIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQG
Sbjct: 301 QAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQG 360
Query: 361 VKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRANKSTMAWGLEAR 420
VKMVLSGEGSDEIFGGYLYFAQAPSAAEFH ESV+RVKNLHLADCLRANKSTMAWGLEAR
Sbjct: 361 VKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVQRVKNLHLADCLRANKSTMAWGLEAR 420
Query: 421 VPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDLKPYLPEEILWRQKE 480
VPFLDR+FLQLCMNIDPN+KMIKP EG+IEKYILRKAFDTT EPD KPYLPEEILWRQKE
Sbjct: 421 VPFLDREFLQLCMNIDPNEKMIKPKEGRIEKYILRKAFDTTGEPDAKPYLPEEILWRQKE 480
Query: 481 QFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYWYRLKFDALFPQKTA 540
QFSDGVGYSWIDGLKD AE+ ISDE FA PKAEWG DIPTTKEA+WYRLKFDALFPQKT
Sbjct: 481 QFSDGVGYSWIDGLKDTAEAVISDEMFASPKAEWGSDIPTTKEAFWYRLKFDALFPQKTV 540
Query: 541 ASTVMRWIPKADWGCAEDPSGRYAKIHEQHVD 572
A TVMRWIPKADWGCAEDPSGRYA+IHE+H++
Sbjct: 541 ADTVMRWIPKADWGCAEDPSGRYAQIHEKHIE 572
>YGR124w [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 572
Score = 994 bits (2571), Expect = 0.0
Identities = 477/571 (83%), Positives = 515/571 (89%), Gaps = 1/571 (0%)
Query: 1 MCGIFASFRNPDVQAYKPKALQLSKRIRHRGPDWSGNIVKNSTILVHERLAIVGLDSGAQ 60
MCGIFA+F++ D+ +KPKALQLSK+IRHRGPDWSGN V NSTI VHERLAIVGLDSGAQ
Sbjct: 1 MCGIFAAFKHEDIHNFKPKALQLSKKIRHRGPDWSGNAVMNSTIFVHERLAIVGLDSGAQ 60
Query: 61 PITSEDGSYTLAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDIDAPKHLDGMF 120
PITS DG Y L VNGEIYNHI LRE C+ Y F+T SDCEPIIPLY ++DIDAPK+LDGMF
Sbjct: 61 PITSADGEYMLGVNGEIYNHIQLREMCSDYKFQTFSDCEPIIPLYLEHDIDAPKYLDGMF 120
Query: 121 AWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIAFPPGHV 180
A+CLYDSKKDRI+AARDPIG+ TLYMGRSS SP++V+F+SELKCLTD CD II+FPPGHV
Sbjct: 121 AFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFASELKCLTDVCDSIISFPPGHV 180
Query: 181 YDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVPYXXXXXXXXXX 240
YDSETDK+TRYFTPDWLDE RIP+TPVD+ A+R SLEKAVRKRLMAEVPY
Sbjct: 181 YDSETDKITRYFTPDWLDEKRIPSTPVDYHAIRHSLEKAVRKRLMAEVPYGVLLSGGLDS 240
Query: 241 XXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGWSRLHSFAVGLPNAPDL 300
IAAIAARETEK NA+ + + D QLAG+D+QGHLH +GWSRLHSFA+GLPNAPDL
Sbjct: 241 SLIAAIAARETEKANADANED-NNVDEKQLAGIDDQGHLHTSGWSRLHSFAIGLPNAPDL 299
Query: 301 QAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQG 360
QAARKVA FIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQG
Sbjct: 300 QAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQG 359
Query: 361 VKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRANKSTMAWGLEAR 420
VKMVLSGEGSDEIFGGYLYFAQAPSAAEFH ESV+RVKNLHLADCLRANKSTMAWGLEAR
Sbjct: 360 VKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVQRVKNLHLADCLRANKSTMAWGLEAR 419
Query: 421 VPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDLKPYLPEEILWRQKE 480
VPFLD+DFLQLCMNIDPN+KMIKP EG+IEKYILRKAFDTTDEPD+KPYLPEEILWRQKE
Sbjct: 420 VPFLDKDFLQLCMNIDPNEKMIKPKEGRIEKYILRKAFDTTDEPDVKPYLPEEILWRQKE 479
Query: 481 QFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYWYRLKFDALFPQKTA 540
QFSDGVGYSWIDGL+D AE ISD FA PKA+WGDDIPTTKEAYWYRLKFDA FPQKTA
Sbjct: 480 QFSDGVGYSWIDGLRDTAERAISDAMFANPKADWGDDIPTTKEAYWYRLKFDAWFPQKTA 539
Query: 541 ASTVMRWIPKADWGCAEDPSGRYAKIHEQHV 571
A TVMRWIPKADWGCAEDPSGRYAKIHE+HV
Sbjct: 540 ADTVMRWIPKADWGCAEDPSGRYAKIHEKHV 570
>SPBC119.10 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 557
Score = 748 bits (1930), Expect = 0.0
Identities = 370/569 (65%), Positives = 435/569 (76%), Gaps = 19/569 (3%)
Query: 1 MCGIFASFR-NPDVQAYKPKALQLSKRIRHRGPDWSGNIVKNSTILVHERLAIVGLDSGA 59
MCGI A D++A+KPKAL LSK++RHRGPDWSG ++N TIL HERLAIVG++SGA
Sbjct: 1 MCGILAVHHVAEDIEAFKPKALHLSKQLRHRGPDWSGKAIRNQTILCHERLAIVGVESGA 60
Query: 60 QPITSEDGSYTLAVNGEIYNHIMLREEC-ASYPFKTLSDCEPIIPLYEKYDIDAPKHLDG 118
QP+ S+DG L VNGEIYNH+ LRE +Y FKT SDCE I+ LY ++ LDG
Sbjct: 61 QPLVSDDGKLVLTVNGEIYNHLKLRENLKGNYKFKTYSDCEVILYLYREHGPACANMLDG 120
Query: 119 MFAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIAFPPG 178
MF+W LYD KD+++AARDPIGITTLY G SS SP + +F+SELK L CD+IIAFPPG
Sbjct: 121 MFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYFASELKALHPVCDKIIAFPPG 180
Query: 179 HVYDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVPYXXXXXXXX 238
H YDSET + RYF P W DE++IP+ PVD+K +RE+LE +VRKRLMAEVPY
Sbjct: 181 HYYDSETKQTVRYFKPSWWDENKIPSNPVDYKLLRETLEASVRKRLMAEVPYGVLLSGGL 240
Query: 239 XXXXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGWSRLHSFAVGLPNAP 298
IA+IAARETEK + + +E + A W +LHSFA+GLP +P
Sbjct: 241 DSSLIASIAARETEKL------------ANSTSQSEEARTITA--WPKLHSFAIGLPGSP 286
Query: 299 DLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKA 358
DL AARKVA F+ + HHEHTFT+ EGLDAL DVIYHLETYDVTTIRASTPM+LLSRKIKA
Sbjct: 287 DLLAARKVADFLHTFHHEHTFTIDEGLDALRDVIYHLETYDVTTIRASTPMYLLSRKIKA 346
Query: 359 QGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRANKSTMAWGLE 418
QGVKMVLSGEGSDEIFGGYLYF APS FH E V+RVKNLHL+DCLRANKSTMAWGLE
Sbjct: 347 QGVKMVLSGEGSDEIFGGYLYFGNAPSREAFHSECVRRVKNLHLSDCLRANKSTMAWGLE 406
Query: 419 ARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDLKPYLPEEILWRQ 478
ARVPFLD+DFL++ +NIDP +KM G+ EKYILRKAFDTT + L+PYLP++ILWRQ
Sbjct: 407 ARVPFLDKDFLEVALNIDPEEKMY--INGRKEKYILRKAFDTTHDSSLQPYLPQDILWRQ 464
Query: 479 KEQFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYWYRLKFDALFPQK 538
KEQFSDGVGYSWID LKD AE ISD++FA P+ EWGDDIPTTKEA+WYR FD +FP++
Sbjct: 465 KEQFSDGVGYSWIDALKDTAELCISDDEFALPRREWGDDIPTTKEAFWYRKLFDEIFPRQ 524
Query: 539 TAASTVMRWIPKADWGCAEDPSGRYAKIH 567
A TVMRW+PKA+WGC EDPSGRY H
Sbjct: 525 -CADTVMRWVPKAEWGCPEDPSGRYQAGH 552
>At5g65010 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 578
Score = 550 bits (1417), Expect = e-156
Identities = 291/579 (50%), Positives = 381/579 (65%), Gaps = 48/579 (8%)
Query: 1 MCGIFASFRNPD-VQAYKPKALQLSKRIRHRGPDWSGNIVKNSTILVHERLAIVGLDSGA 59
MCGI A D QA + + ++LS+R+RHRGPDWSG L HERLAI+ SG
Sbjct: 1 MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60
Query: 60 QPITSEDGSYTLAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDIDAPKHLDGM 119
QP+ +ED + + VNGEIYNH +LRE+ S+ F+T SDCE I LYE++ + LDGM
Sbjct: 61 QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120
Query: 120 FAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIAFPPGH 179
FA+ L D++ IAARD IGIT LY+G SVWF+SE+K L+DDC++ ++FPPGH
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG--SVWFASEMKALSDDCEQFMSFPPGH 178
Query: 180 VYDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVPYXXXXXXXXX 239
+Y S+ L R++ P W +E ++P+TP D +R + EKAV KRLM +VP+
Sbjct: 179 IYSSKQGGLRRWYNPPWYNE-QVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLD 237
Query: 240 XXXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGW-SRLHSFAVGLPNAP 298
+AA+A R EK+ A A W S+LH+F +GL +P
Sbjct: 238 SSLVAAVALRHLEKSEA------------------------ARQWGSQLHTFCIGLQGSP 273
Query: 299 DLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKA 358
DL+A R+VA ++G+ HHE FT+Q+G+DA+++VIYH+ETYDVTTIRASTPMFL+SRKIK+
Sbjct: 274 DLKAGREVADYLGTRHHEFQFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKS 333
Query: 359 QGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRANKSTMAWGLE 418
GVKMVLSGEGSDEI GGYLYF +AP+ EFH E+ +++K LH DCLRANKST AWG+E
Sbjct: 334 LGVKMVLSGEGSDEILGGYLYFHKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVE 393
Query: 419 ARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDLKPYLPEEILWRQ 478
ARVPFLD++FL + M+IDP K+IKP G+IEK++LR AFD + +PYLP+ IL+RQ
Sbjct: 394 ARVPFLDKEFLNVAMSIDPEWKLIKPDLGRIEKWVLRNAFDDEE----RPYLPKHILYRQ 449
Query: 479 KEQFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYWYRLKFDALFPQK 538
KEQFSDGVGYSWIDGLKD A +SD + + D+ P TKEAY+YR F+ FP+
Sbjct: 450 KEQFSDGVGYSWIDGLKDHANKHVSDTMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKS 509
Query: 539 TAASTV-------------MRWIPKADWGCAEDPSGRYA 564
A +TV + W A W DPSGR A
Sbjct: 510 AARATVPGGPSIACSTAKAVEW--DATWSKNLDPSGRAA 546
>At5g10240 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 578
Score = 546 bits (1406), Expect = e-155
Identities = 293/579 (50%), Positives = 377/579 (64%), Gaps = 48/579 (8%)
Query: 1 MCGIFASFRNPD-VQAYKPKALQLSKRIRHRGPDWSGNIVKNSTILVHERLAIVGLDSGA 59
MCGI A D QA + + ++LS+R+RHRGPDWSG L HERLAIV SG
Sbjct: 1 MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60
Query: 60 QPITSEDGSYTLAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDIDAPKHLDGM 119
QP+ +ED + + VNGEIYNH LRE S+ F+T SDCE I LYE++ + LDGM
Sbjct: 61 QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120
Query: 120 FAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIAFPPGH 179
FA+ L D++ IAARD IGIT LY+G SVWF+SE+K L+DDC++ + FPPGH
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG--SVWFASEMKALSDDCEQFMCFPPGH 178
Query: 180 VYDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVPYXXXXXXXXX 239
+Y S+ L R++ P W E +P+TP D VR + EKAV KRLM +VP+
Sbjct: 179 IYSSKQGGLRRWYNPPWFSEV-VPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLD 237
Query: 240 XXXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGW-SRLHSFAVGLPNAP 298
+A++A R EK+ A A W S+LH+F +GL +P
Sbjct: 238 SSLVASVALRHLEKSEA------------------------ACQWGSKLHTFCIGLKGSP 273
Query: 299 DLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKA 358
DL+A R+VA ++G+ HHE FT+Q+G+DA+++VIYH+ETYDVTTIRASTPMFL+SRKIK+
Sbjct: 274 DLKAGREVADYLGTRHHELHFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKS 333
Query: 359 QGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRANKSTMAWGLE 418
GVKMVLSGEGSDEIFGGYLYF +AP+ EFH E+ +++K LH DCLRANKST AWG+E
Sbjct: 334 LGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVE 393
Query: 419 ARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDLKPYLPEEILWRQ 478
ARVPFLD++F+ + M+IDP KMI+P G+IEK++LR AFD DE + PYLP+ IL+RQ
Sbjct: 394 ARVPFLDKEFINVAMSIDPEWKMIRPDLGRIEKWVLRNAFD--DEKN--PYLPKHILYRQ 449
Query: 479 KEQFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYWYRLKFDALFPQK 538
KEQFSDGVGYSWIDGLKD A +S+ + D+ P TKEAY+YR F+ FP+
Sbjct: 450 KEQFSDGVGYSWIDGLKDHANKHVSETMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKS 509
Query: 539 TAASTV-------------MRWIPKADWGCAEDPSGRYA 564
A +TV + W A W DPSGR A
Sbjct: 510 AARATVPGGPSVACSTAKAVEW--DAAWSQNLDPSGRAA 546
>At3g47340 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 584
Score = 541 bits (1394), Expect = e-153
Identities = 282/579 (48%), Positives = 376/579 (64%), Gaps = 48/579 (8%)
Query: 1 MCGIFASFR-NPDVQAYKPKALQLSKRIRHRGPDWSGNIVKNSTILVHERLAIVGLDSGA 59
MCGI A + D QA + + L+LS+R+RHRGPDWSG L H+RLA++ SG
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60
Query: 60 QPITSEDGSYTLAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDIDAPKHLDGM 119
QP+ +ED + + VNGEIYNH LR+ ++ F+T SDCE I LYE+Y +D LDG+
Sbjct: 61 QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120
Query: 120 FAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIAFPPGH 179
F++ L D++ + + ARD IG+T+LY+G SVW SSE+K L DDC+ FPPGH
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDG--SVWISSEMKGLNDDCEHFETFPPGH 178
Query: 180 VYDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVPYXXXXXXXXX 239
Y S+ +++ P W +ES +P+TP + A+R + E AV KRLM +VP+
Sbjct: 179 FYSSKLGGFKQWYNPPWFNES-VPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLD 237
Query: 240 XXXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGWS-RLHSFAVGLPNAP 298
+A+I AR T A A W +LHSF VGL +P
Sbjct: 238 SSLVASITARHLAGTKA------------------------AKQWGPQLHSFCVGLEGSP 273
Query: 299 DLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKA 358
DL+A ++VA ++G++HHE F++Q+G+DA++DVIYH+ETYDVTTIRASTPMFL+SRKIK+
Sbjct: 274 DLKAGKEVAEYLGTVHHEFHFSVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKS 333
Query: 359 QGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRANKSTMAWGLE 418
GVKMVLSGEG+DEIFGGYLYF +AP+ EFH E+ +++K LH DCLRANKST A+GLE
Sbjct: 334 LGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLE 393
Query: 419 ARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDLKPYLPEEILWRQ 478
ARVPFLD+DF+ M++DP KMIKP EG+IEK++LR+AFD + +PYLP+ IL+RQ
Sbjct: 394 ARVPFLDKDFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEE----RPYLPKHILYRQ 449
Query: 479 KEQFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYWYRLKFDALFPQK 538
KEQFSDGVGYSWIDGLKD A ++D+ + + + P TKEAY+YR+ F+ FPQ
Sbjct: 450 KEQFSDGVGYSWIDGLKDHAAQNVNDKMMSNAGHIFPHNTPNTKEAYYYRMIFERFFPQN 509
Query: 539 TAASTV-------------MRWIPKADWGCAEDPSGRYA 564
+A TV + W A W DPSGR A
Sbjct: 510 SARLTVPGGATVACSTAKAVEW--DASWSNNMDPSGRAA 546
>CE04760 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 567
Score = 419 bits (1078), Expect = e-117
Identities = 253/582 (43%), Positives = 349/582 (59%), Gaps = 58/582 (9%)
Query: 1 MCGIFASFRNPDVQAYKPK-----ALQLSKRIRHRGPDWSG--NIVKNSTILVHERLAIV 53
MCG+F+ + KPK A +LS+R HRGPD+SG K ILVHERLAI+
Sbjct: 1 MCGVFSICQQTCKN--KPKYDLDEARKLSRRQHHRGPDFSGCYQDTKTGDILVHERLAIM 58
Query: 54 GLDSGAQPITSEDGSYTLAVNGEIYNHIMLRE-ECASYPFKTLSDCEPIIPLYEKY-DID 111
L QP+ + + NGEIYNH LR+ E Y KT D E II LYEKY D
Sbjct: 59 DLGV-VQPLQGTAPNRQVIHNGEIYNHQELRDTELKKYNLKTHCDSEVIIFLYEKYRDGH 117
Query: 112 APKHLDGMFAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDC-- 169
LDG+FA+ L +AARDP+G+ +Y G +FS+E+KCL D C
Sbjct: 118 ICNLLDGVFAFVLCCDGD--FLAARDPLGVKQMYYGIDDDG--RYFFSNEMKCLEDSCGD 173
Query: 170 DEIIAFPPGHVYDSETDKLTRYFTPDWLDESRIPTTPVDFKAVRESLEKAVRKRLMAEVP 229
++I +FPPGH Y +T RYF P+W D R P+D K +++++ +V KRLM++ P
Sbjct: 174 NKIESFPPGHYYTPKTG-FVRYFNPEWFD-FRKAIHPLDLKLLQKTMIASVHKRLMSDAP 231
Query: 230 YXXXXXXXXXXXXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGWSRLHS 289
+++IA+RE ++ + HS
Sbjct: 232 IGVLLSGGLDSSLVSSIASREMKRRGMAV-----------------------------HS 262
Query: 290 FAVGLP-NAPDLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTP 348
F++G+ N+PD+ AARKVA FIG+ HHE F+++EG+ L +I+HLE+YDVT+IRASTP
Sbjct: 263 FSIGVDHNSPDVVAARKVAKFIGTTHHEFYFSIEEGIKNLRKLIWHLESYDVTSIRASTP 322
Query: 349 MFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCLRA 408
M+ LS +I+ G+K+VLSGEG+DEIFGGYLYF APS +F E++ RV +L+ +DCLRA
Sbjct: 323 MYFLSEEIRKLGIKVVLSGEGADEIFGGYLYFHNAPSDEDFQKETIDRVLHLYTSDCLRA 382
Query: 409 NKSTMAWGLEARVPFLDRDFLQLCMNIDPNDKMIKPAEG--KIEKYILRKAFDTTDEPDL 466
+KS+MA +E RVPFLD+ F++ +++DP K + E EK++LR AF+T D
Sbjct: 383 DKSSMAHSVEVRVPFLDKAFVEAAVSLDPAFKRPQKLEDGRNCEKFVLRSAFNT----DQ 438
Query: 467 KPYLPEEILWRQKEQFSDGVGYSWIDGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAYW 526
PYLP+EILWRQKEQFSDGVGYSWID L + I+D++F+Q + + P +KEA++
Sbjct: 439 YPYLPDEILWRQKEQFSDGVGYSWIDTLMKYCAAKITDKEFSQASKLFPHNTPHSKEAFY 498
Query: 527 YRLKFDALFPQKTAASTVMRWIPKADWGCAEDPSGRYAKIHE 568
R F FP + AA TV +WIPK W +DPSGR + +HE
Sbjct: 499 MRKIFHEQFPSEQAALTVRKWIPK--WQKNQDPSGRASLVHE 538
>Hs19718772 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 561
Score = 249 bits (636), Expect = 8e-66
Identities = 193/594 (32%), Positives = 295/594 (49%), Gaps = 76/594 (12%)
Query: 1 MCGIFASFRNPDVQAYKPKALQLSKRIRHRGPDW----SGNIVKNSTILVHERLAIVGLD 56
MCGI+A F + D + + + + +I HRGPD + N N H RLA+V
Sbjct: 1 MCGIWALFGSDDCLSVQCLS---AMKIAHRGPDAFRFENVNGYTNCCFGFH-RLAVVDPL 56
Query: 57 SGAQPITSEDGSYT-LAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDIDAPK- 114
G QPI + Y L NGEIYNH +++ + ++T D E I+ LY+K I+
Sbjct: 57 FGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHF-EFEYQTKVDGEIILHLYDKGGIEQTIC 115
Query: 115 HLDGMFAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSE----LKCLTDDCD 170
LDG+FA+ L D+ ++ RD G+ L+ + +V ++ LK
Sbjct: 116 MLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFL 175
Query: 171 EIIAFPPGHVYDSETDKLTRYFTPDWLDESRIPTTPV--------------DFKAVRESL 216
++ F PGH + + + + + P+ + + V+ +L
Sbjct: 176 KVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDNVEKLFPGFEIETVKNNL 235
Query: 217 E----KAVRKRLMAEVPYXXXXXXXXXXXXIAAIAARETEKTNAELDPVVYDSDPHQLAG 272
AV+KRLM + +AA ++ ++ +
Sbjct: 236 RILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYP------------- 282
Query: 273 LDEQGHLHAAGWSRLHSFAVGLPNAPDLQAARKVATFIGSIHHEHTFTLQEGLDALDDVI 332
L +FA+G+ ++PDL AARKVA IGS H+E F +EG+ ALD+VI
Sbjct: 283 --------------LQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVI 328
Query: 333 YHLETYDVTTIRASTPMFLLSRKIKAQGVKMVL-SGEGSDEIFGGYLYFAQAPSAAEFHV 391
+ LETYD+TT+RAS M+L+S+ I+ +V+ SGEGSDE+ GY+YF +APS +
Sbjct: 329 FSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE 388
Query: 392 ESVKRVKNLHLADCLRANKSTMAWGLEARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEK 451
ES + ++ L+L D LRA+++T A GLE RVPFLD F +++ P +M P G IEK
Sbjct: 389 ESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPP--EMRIPKNG-IEK 445
Query: 452 YILRKAFDTTDEPDLKPYLPEEILWRQKEQFSDG---VGYSWIDGLKDAAESTISDEQFA 508
++LR+ F+ ++ +P+EILWR KE FSDG V SW L++ E + D A
Sbjct: 446 HLLRETFEDSN------LIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMA 499
Query: 509 QPKAEWGDDIPTTKEAYWYRLKFDALFPQKTAASTVMRWIPKADWGCAEDPSGR 562
++ + P TKE Y+YR F+ +P + A W+PK W A DPS R
Sbjct: 500 NAAQKFPFNTPKTKEGYYYRQVFERHYPGR-ADWLSHYWMPK--WINATDPSAR 550
>CE09642 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 551
Score = 243 bits (621), Expect = 4e-64
Identities = 182/550 (33%), Positives = 272/550 (49%), Gaps = 70/550 (12%)
Query: 1 MCGIFASFRNPDVQAYKPKALQLSKRIRHRGPDWSG-NIVKNSTILVHERLAIV--GLDS 57
MCGI+A + P+ +I RGPD + V+ + L RLAIV G
Sbjct: 1 MCGIWAIIG----EQVTPQQQDAFMKIVGRGPDLTVLEEVQPNVHLGFHRLAIVMPGDTP 56
Query: 58 GAQPITSEDGSYTLAVNGEIYNHIMLREECASYPFKTLSDCEPIIPLYEKYDID---APK 114
AQPI G ++ NGEIYNH L+ +C SDC II + K++ D A
Sbjct: 57 SAQPIVG--GGLSVVCNGEIYNHQSLKIDCPFTLKNGGSDCAAIITSFIKHNKDLKEACA 114
Query: 115 HLDGMFAWCLYDSKKDRIIAARDPIGITTLYMGRSSASPKSVWFSSELKCLTDDCDEIIA 174
LDG+FA+ + D K + RDPIG+ L+ G +S S+ SE+KC+ + C+ +
Sbjct: 115 SLDGVFAFIMADDKN--VYIGRDPIGVRPLFYGYNSNG--SLLIGSEVKCIEELCERVEY 170
Query: 175 FPPGHVYDSETDKLTRYFTPDWLDESRIPTTPVDFKA-------VRESLEKAVRKRLMAE 227
FPPG TR P +PT F + VR+ L K+V KRLM
Sbjct: 171 FPPGCCATISLFAPTRPVQPQ--QYYSVPTIADRFLSIDCTKTLVRDVLVKSVEKRLMGN 228
Query: 228 VPYXXXXXXXXXXXXIAAIAARETEKTNAELDPVVYDSDPHQLAGLDEQGHLHAAGWSRL 287
+ IA+IA R ++ P+
Sbjct: 229 RNFGFMLSGGLDSSLIASIATRFLKQK-----PI-------------------------- 257
Query: 288 HSFAVGLPNAPDLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRAST 347
+F+VG ++PDL+ A+KVA ++ H T Q+ +D + +V++ LET+D IR
Sbjct: 258 -AFSVGFEDSPDLENAKKVADYLKIPHEVLVITPQQCIDIIPEVVFALETFDPLIIRCGI 316
Query: 348 PMFLLSRKI-KAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHVESVKRVKNLHLADCL 406
+LL + I K+ VK++LSGEG+DE+FG Y Y +AP+A H E ++R+ +LH D L
Sbjct: 317 AHYLLCQHISKSSDVKVLLSGEGADELFGSYAYMQRAPNALHLHKEILRRMHHLHQYDVL 376
Query: 407 RANKSTMAWGLEARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTDEPDL 466
R ++ST GLE RVPFLD+ F+ L + P+ K++ K+EK++LR AF+
Sbjct: 377 RCDRSTSCHGLEIRVPFLDKRFIDLVSRLPPSYKLMPM---KLEKHVLRSAFE------- 426
Query: 467 KPYLPEEILWRQKEQFSDGVGYSWI-DGLKDAAESTISDEQFAQPKAEWGDDIPTTKEAY 525
+LP+E+LWR KE F++ +G + + D L + + IS + + + D P T E Y
Sbjct: 427 -GWLPDEVLWRSKEGFAEALGKTDLGDILAEHCDKLISKSLYERRAERFPDRTPETTEEY 485
Query: 526 WYRLKFDALF 535
WYR F+ F
Sbjct: 486 WYRQIFEDTF 495
>Hs20539333 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 430
Score = 125 bits (313), Expect = 2e-28
Identities = 78/217 (35%), Positives = 119/217 (53%), Gaps = 7/217 (3%)
Query: 287 LHSFAVGLPNAPDLQAARKVATFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRAS 346
L +F +G+ ++PDL AARKVA IGS HHE F +EG ALD+VI+ LETY++TT AS
Sbjct: 202 LQTFVIGMEDSPDLLAARKVANHIGSEHHEVLFNSEEGFQALDEVIFSLETYNITTADAS 261
Query: 347 TPMFLLSRKIKAQGVKMVL-SGEGSDEIFGG---YLYFAQAPSAAEFHVESVKRVKNLHL 402
M+L+S+ I+ +V+ SGEGSDE+ L F + + + ES + ++ L+L
Sbjct: 262 VSMYLISKYIRKNTDSVVIFSGEGSDELIQAKLMVLLFVKWAPSEKAKEESERLLRELYL 321
Query: 403 ADCLRANKSTMAWGLEARVPFLDRDFLQLCMNIDPNDKMIKPAEGKIEKYILRKAFDTTD 462
D L A+++T A LE RVPFLD F + + ++ K + + + F+T
Sbjct: 322 FDVLHADQTTAAHSLELRVPFLDHQFSSYYLYLSTEMRIPKVDDAMMANAAQKFPFNT-- 379
Query: 463 EPDLKPYLPEEILWRQKEQFSDGVGYSWIDGLKDAAE 499
P K Y +IL +D + + W+ DA +
Sbjct: 380 -PKTKGYYYHQILEHHFPGQADWLSHYWVPKWIDATD 415
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,823,018
Number of Sequences: 60738
Number of extensions: 1549381
Number of successful extensions: 3251
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3197
Number of HSP's gapped (non-prelim): 13
length of query: 574
length of database: 30,389,216
effective HSP length: 112
effective length of query: 462
effective length of database: 23,586,560
effective search space: 10896990720
effective search space used: 10896990720
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)