ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2763 good J KOG2154 Translation, ribosomal structure and biogenesis Predicted nucleolar protein involved in ribosome biogenesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2763 954119 955681 521
(521 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR144c [J] KOG2154 Predicted nucleolar protein involved in ribo... 520 e-147
SPBC1604.06c [J] KOG2154 Predicted nucleolar protein involved in... 265 1e-70
Hs13129068 [J] KOG2154 Predicted nucleolar protein involved in r... 216 5e-56
7290427 [J] KOG2154 Predicted nucleolar protein involved in ribo... 174 4e-43
CE01409 [J] KOG2154 Predicted nucleolar protein involved in ribo... 130 5e-30
At2g17250 [J] KOG2154 Predicted nucleolar protein involved in ri... 74 7e-13
YDR060w [JK] KOG2038 CAATT-binding transcription factor/60S ribo... 59 1e-08
At1g79140 [JU] KOG2153 Protein involved in the nuclear export of... 53 1e-06
Hs20806097 [JU] KOG2153 Protein involved in the nuclear export o... 50 8e-06
>YPR144c [J] KOG2154 Predicted nucleolar protein involved in ribosome
biogenesis
Length = 552
Score = 520 bits (1340), Expect = e-147
Identities = 286/550 (52%), Positives = 364/550 (66%), Gaps = 38/550 (6%)
Query: 6 EIKQVWKSISTGNDRKHYNSLVNLIHGYXXXXXXXXXXXXXXD-DYRYMTIALSQIFIKL 64
EIK + K ++ DRK YNS++ LI+ + + R++ ++L QIF KL
Sbjct: 7 EIKDIAKRLTAAGDRKQYNSIIKLINELVIPENVTQLEEDETEKNLRFLVMSLFQIFRKL 66
Query: 65 FERGQL-MPHLASNANEKK-FFEWCXXXXXXXXXXXXAIITSLHEVNSLSLDCLDSYMKM 122
F RG L +P + EK+ F WC AII+ + SL LD LD Y+++
Sbjct: 67 FSRGDLTLPSSKKSTLEKEQFVNWCRKVYEAFKTKLLAIISDIPFETSLGLDSLDVYLQL 126
Query: 123 LHHESIYWASKPNSPFFPNKTLRSLLVALFHCNFEGDIGF--KDGQSENPVFNLFVEAYY 180
ES ++AS+ +PFFPNKT R L++AL+ N G+I G SEN + F E YY
Sbjct: 127 AELESTHFASEKGAPFFPNKTFRKLIIALWSSNM-GEIEDVKSSGASENLIIVEFTEKYY 185
Query: 181 KKNFDIQFYTQSELISLLPELDGQTSDEIL--MSKWLALCNNDVQGSP---DLEIFVSVP 235
K DIQ+Y QSE LL D D +L + KWLAL N+D S DLEIFV P
Sbjct: 186 TKFADIQYYFQSEFNQLLE--DPAYQDLLLKNVGKWLALVNHDKHCSSVDADLEIFVPNP 243
Query: 236 PQAIENDAKFKSNLETNWLHCLNLANLSSSQYKTVLLVLHKRIIVWFHQPTKLMDFLTDS 295
PQAIEN++KFKSN E NWL LN LS QYK++LL+LHKRII FH PTKLMDFLTDS
Sbjct: 244 PQAIENESKFKSNFEKNWLSLLN-GQLSLQQYKSILLILHKRIIPHFHTPTKLMDFLTDS 302
Query: 296 YN------QGGIVSILSLNGLFELMKKYNLEYPNFYSKLYQLLTPEVMHVKYRSRFFRLV 349
YN G+V IL+LNGLFELMK++NLEYPNFY KLYQ++ P++MHVKYR+RFFRL+
Sbjct: 303 YNLQSSNKNAGVVPILALNGLFELMKRFNLEYPNFYMKLYQIINPDLMHVKYRARFFRLM 362
Query: 350 DTFLSSTHLSAQLVASFIKRLARLSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDPEYI 409
D FLSSTHLSA LVASFIK+LARL++ +PP AIV++IPF+YNLIRKHP CMI+LH+P +I
Sbjct: 363 DVFLSSTHLSAHLVASFIKKLARLTLESPPSAIVTVIPFIYNLIRKHPNCMIMLHNPAFI 422
Query: 410 DCDSKK----------RQEYLDPFNNEETNPELTNALSSSVWELETLMNHYHPNVATLAK 459
+ ++ Y+DPF+ E++PELT+AL SS+WEL +LM HYHPNVATLAK
Sbjct: 423 SNPFQTPDQVANLKTLKENYVDPFDVHESDPELTHALDSSLWELASLMEHYHPNVATLAK 482
Query: 460 IFQQPFQKLSYNIEDFLDWSYDSLLQAETNRKLKIQPALEFERFETIFE--------SGS 511
IF QPF+KLSYN+EDFLDW+YDSLL AE++RKLK P LEFE F +F+ S
Sbjct: 483 IFAQPFKKLSYNMEDFLDWNYDSLLNAESSRKLKTLPTLEFEAFTNVFDNEDGDSEASSQ 542
Query: 512 DSAFLPFIKW 521
+ +LP + W
Sbjct: 543 GNVYLPGVAW 552
>SPBC1604.06c [J] KOG2154 Predicted nucleolar protein involved in ribosome
biogenesis
Length = 485
Score = 265 bits (678), Expect = 1e-70
Identities = 166/420 (39%), Positives = 233/420 (54%), Gaps = 53/420 (12%)
Query: 102 ITSLHEVNSLSLDCLDSYMKMLHHESIYWASKPNSPFFPNKTLRSLLVALFHCNFEGDIG 161
I S+ V +L + L M++ ES + F N+ + L + DI
Sbjct: 88 IFSMANVEALQVSFLTMTMRLCKAES----QMDENGTFRNQFYIRFCLELLSSSQLSDIC 143
Query: 162 FKDGQSENPVFNLFVEAYYKKNFDIQFY---TQSELISLLPELDGQTSDEILMSKWLALC 218
KD FV +Y D++F+ ++IS L E +T D +
Sbjct: 144 IKD----------FVTSYLVPYDDVRFFFYKNSKKVISSLIE-SSKTDDP--------MA 184
Query: 219 NNDVQGSPDLEIFVSVP----------------PQAIENDAKFKSNLETNWLHCLNLANL 262
N D+ + I ++P P + E + K + +WL L+L L
Sbjct: 185 NLDIVAFNTIRILSAIPSPLPSSSTSSWADEPSPSSTETSS-IKRAFQESWLSALSLP-L 242
Query: 263 SSSQYKTVLLVLHKRIIVWFHQPTKLMDFLTDSYNQGGIVSILSLNGLFELMKKYNLEYP 322
S + YK VL V+HKR+I + +P LMDFLTD+YN VS+L+LNGLF LM +NL+YP
Sbjct: 243 SVNLYKQVLNVIHKRVIPFLQKPNLLMDFLTDAYNSHHAVSLLALNGLFTLMISHNLDYP 302
Query: 323 NFYSKLYQLLTPEVMHVKYRSRFFRLVDTFLSSTHLSAQLVASFIKRLARLSVSAPPGAI 382
FY KLY LL ++++K RSRFFRL+D FLSSTHL A L+ASFIKRLARL+++APPGAI
Sbjct: 303 LFYPKLYALLDRNLLYLKTRSRFFRLLDLFLSSTHLPATLIASFIKRLARLALTAPPGAI 362
Query: 383 VSIIPFVYNLIRKHPTCMILLHDPEYIDCDSKKRQEYLDPFNNEETNPELTNALSSSVWE 442
+IPF+YN +++HPTCM +LH + E D F+ ++ +P LT A+ SS+WE
Sbjct: 363 AIVIPFIYNCLQRHPTCMQMLH---------RSSAESGDSFDFDQPDPLLTGAIESSLWE 413
Query: 443 LETLMNHYHPNVATLAKIFQQPFQKLSYNIEDFLDWSYDSLLQAETNRKLKIQPALEFER 502
L TL NHY+ N+A+LA I Q F K Y +EDFLD Y ++ AE R LK +P +EFE+
Sbjct: 414 LSTLQNHYYSNIASLASIMSQKFTKPRYELEDFLDHGYATMCDAELRRPLKNEPPIEFEK 473
>Hs13129068 [J] KOG2154 Predicted nucleolar protein involved in ribosome
biogenesis
Length = 516
Score = 216 bits (551), Expect = 5e-56
Identities = 112/248 (45%), Positives = 157/248 (63%), Gaps = 8/248 (3%)
Query: 253 WLHCLNLANLSSSQYKTVLLVLHKRIIVWFHQPTKLMDFLTDSYNQGGIVSILSLNGLFE 312
WL L L S YK VLL++H I+ QPT ++DFLT + + GG +S+L+LNGLF
Sbjct: 256 WLSFLK-HKLPLSLYKKVLLIVHDAILPQLAQPTLMIDFLTRACDLGGALSLLALNGLFI 314
Query: 313 LMKKYNLEYPNFYSKLYQLLTPEVMHVKYRSRFFRLVDTFLSSTHLSAQLVASFIKRLAR 372
L+ K+NLEYP+FY KLY LL P V HVKYR+RFF L D FLSS+HL A LVA+F KRLAR
Sbjct: 315 LIHKHNLEYPDFYRKLYGLLDPSVFHVKYRARFFHLADLFLSSSHLPAYLVAAFAKRLAR 374
Query: 373 LSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDPEYIDCDSKKRQEYLDPFNNEETNPEL 432
L+++APP A++ ++PF+ NL+R+HP C +L+H P + D+ DP++ E +P
Sbjct: 375 LALTAPPEALLMVLPFICNLLRRHPACRVLVHRPHGPELDA-------DPYDPGEEDPAQ 427
Query: 433 TNALSSSVWELETLMNHYHPNVATLAKIFQQPFQKLSYNIEDFLDWSYDSLLQAETNRKL 492
+ AL SS+WEL+ L HYHP V+ A + Q +I L+ + + + + +K
Sbjct: 428 SRALESSLWELQALQRHYHPEVSKAASVINQALSMPEVSIAPLLELTAYEIFERDLKKKG 487
Query: 493 KIQPALEF 500
LEF
Sbjct: 488 PEPVPLEF 495
>7290427 [J] KOG2154 Predicted nucleolar protein involved in ribosome
biogenesis
Length = 497
Score = 174 bits (440), Expect = 4e-43
Identities = 114/332 (34%), Positives = 174/332 (52%), Gaps = 22/332 (6%)
Query: 180 YKKNFDIQFYTQSELISLLPELDGQTSDEILMSKWLALCNNDVQGSPDLEIFVSVPPQAI 239
Y KN D+ T ++L SL+P+ D + S I +L++ N G L S +
Sbjct: 160 YCKNLDVLEITLTQLKSLVPDEDFKDSP-IKAMNFLSIVNLLDMGKSVL----STADYHV 214
Query: 240 ENDAKFKSNLETN-------WLHCLNLAN-LSSSQYKTVLLVLHKRIIVWFHQPTKLMDF 291
E+ K N E N W + N + ++ +L+VL +R+I + P +L DF
Sbjct: 215 ESAKKQTFNYELNRKRLNNLWATIMAKGNEVDEKLHRQILVVLLERVINHLNDPIQLTDF 274
Query: 292 LTDSYNQ-GGIVSILSLNGLFELMKKYNLEYPNFYSKLYQLLTPEVMHVKYRSRFFRLVD 350
L DS +Q G +++L+L G+F LM+K N+ YP+ Y KLY + P + H KY++R F L D
Sbjct: 275 LMDSLHQFDGPIALLALQGIFTLMQKQNITYPDVYEKLYNMFYPRMFHNKYKARLFYLAD 334
Query: 351 TFLSSTHLSAQLVASFIKRLARLSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDPEYID 410
FL+STHL LVA+F+KRLARL++ +P V +I FV NL+ +H L+ +D
Sbjct: 335 IFLTSTHLPENLVAAFVKRLARLALQSPTEDAVIMIRFVCNLLLRHTGLQKLIRASHAVD 394
Query: 411 CDSKKRQEYLDPFNNEETNPELTNALSSSVWELETLMNHYHPNVATLAKIFQQPFQKLSY 470
E DP+N ET+P + A++SS+WE+ L H P VA A+ + +
Sbjct: 395 -------EVSDPYNQTETDPVKSEAINSSLWEITLLQKHVVPEVANAARFINSSLPVMEF 447
Query: 471 NIEDFLDWSYDSLLQAETNRKLKIQPALEFER 502
++ LD ++ E K K Q AL +ER
Sbjct: 448 DLAPLLDRKECNIFDDELQSKAK-QFALNYER 478
>CE01409 [J] KOG2154 Predicted nucleolar protein involved in ribosome
biogenesis
Length = 504
Score = 130 bits (327), Expect = 5e-30
Identities = 71/216 (32%), Positives = 115/216 (52%), Gaps = 8/216 (3%)
Query: 253 WLHCLNLANLSSSQYKTVLLVLHKRIIVWFHQPTKLMDFLTDSYNQGGIVSILSLNGLFE 312
W+ +N +S ++ + + +I P K DF +++ ILSL +F
Sbjct: 198 WMAVMN-GKISDKLTLKLIPYITQNVISKLKAPFKSADFFFKMFDKTDYHGILSLGAIFR 256
Query: 313 LMKKYNLEYPNFYSKLYQLLTPEVMHVKYRSRFFRLVDTFLSSTHLSAQLVASFIKRLAR 372
L+ ++N EYP FY K+Y L P ++++ + L+D+FLSSTHL + ASF+KRL+R
Sbjct: 257 LISEHNFEYPKFYDKVYSLTNPSLLYMSQKESILTLLDSFLSSTHLPTYITASFLKRLSR 316
Query: 373 LSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDPEYIDCDSKKRQEYLDPFNNEETNPEL 432
+ AP A I+ + NL+ +HP C L+H + Y DPF+N+ET+
Sbjct: 317 CLLLAPIDAQEPILGLIRNLVIRHPNCSELVH-------REVPQTLYDDPFDNDETDLHK 369
Query: 433 TNALSSSVWELETLMNHYHPNVATLAKIFQQPFQKL 468
T AL SS+WE++ L H++ +V A + QK+
Sbjct: 370 TRALESSLWEMKLLQCHWNQSVRKRAHFVDKSIQKI 405
>At2g17250 [J] KOG2154 Predicted nucleolar protein involved in ribosome
biogenesis
Length = 456
Score = 73.6 bits (179), Expect = 7e-13
Identities = 60/204 (29%), Positives = 90/204 (43%), Gaps = 56/204 (27%)
Query: 287 KLMDFLTDSYNQGGIVSILSLNGLFELMKKYNLEYPNFYSKLYQLLTPEVMHVKYRSRFF 346
KL DFLT SY+ GG+VS+++L+ LF LM ++ LEYP FY KLY LL P V K+R++F
Sbjct: 265 KLNDFLTKSYDIGGVVSVMALSSLFILMTQHGLEYPFFYEKLYALLVPSVFVAKHRAKFL 324
Query: 347 RLVDTFLSSTHLSAQLVASFIKRLARLSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDP 406
+ + + A + S P I+K
Sbjct: 325 QEIVENADEANTEA----------GEHNESQP------------KTIKK----------- 351
Query: 407 EYIDCDSKKRQEYLDPFNNEETNPELTNALSSSVWELETLMNHYHPNVATLAKIFQQPFQ 466
R+ +D FNN+E++P+ + ALS + LET N+ +K +
Sbjct: 352 ---------RKLGIDYFNNQESDPKKSGALSKFISSLET-------NLTIRSKTTEM--- 392
Query: 467 KLSYNIEDFLDWSYDSLLQAETNR 490
IEDF SY ++ E +
Sbjct: 393 ----KIEDFCSGSYATIFGDEKGK 412
>YDR060w [JK] KOG2038 CAATT-binding transcription factor/60S ribosomal
subunit biogenesis protein
Length = 1025
Score = 59.3 bits (142), Expect = 1e-08
Identities = 69/281 (24%), Positives = 113/281 (39%), Gaps = 44/281 (15%)
Query: 240 ENDAKFKSNLETNWLHCLNLANLSSSQYKTVLLVLHKRIIVWFHQPTKLMDFLTDSYNQG 299
E ++K S L T A + +S Y+ + L K +T S N
Sbjct: 551 EKNSKLFSALLTGINRAFPFAQIPASVYEVHMETLFK---------------ITHSSNFN 595
Query: 300 GIVSILSLNGLFELMKKYNLEYPNFYSKLYQ-LLTPEVMHVKYRSRFFRLVDTFLSSTHL 358
+ L L + ++ K L +Y LY+ L P +++ + + L+ L L
Sbjct: 596 TSIQALVL--INQVTVKAKLNSDRYYRTLYESLFDPRLVNSSKQGIYLNLLYKSLKQDAL 653
Query: 359 SAQLVASFIKRLAR-----LSVSAPPG------AIVSIIPFVYNLIRKHPTCMILLHDPE 407
+ + V +F+KR+ + L+V G + +P + NL+ P D E
Sbjct: 654 NVERVEAFVKRILQVCSHWLNVGTITGFFFLLIQLAKTVPQIKNLLTNTPVDYEYESDAE 713
Query: 408 YIDCDSK-KRQEYLDPFNNEETNPELTNALSSSVWELETLMNHYHPNVATLAKIF----- 461
D KR+EY + + +P+ NA SS+WE+ +NH+HP V T A +
Sbjct: 714 EEQGDKDIKRKEY----DGRKRDPKFANAEKSSLWEINNFINHFHPTVKTYANAYVTGET 769
Query: 462 ---QQPFQKLSYNIEDFLD-WSYDSLLQAETNRKLKIQPAL 498
+P L + + FLD + Y S Q T R I L
Sbjct: 770 EQIAKPDLGL-FTLSHFLDRFVYRSAKQTNTARGTSIMQPL 809
>At1g79140 [JU] KOG2153 Protein involved in the nuclear export of
pre-ribosomes
Length = 311
Score = 52.8 bits (125), Expect = 1e-06
Identities = 30/102 (29%), Positives = 51/102 (49%), Gaps = 4/102 (3%)
Query: 357 HLSAQLVASFIKRLARLSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDPEYIDCDSKKR 416
H Q A+F+KRLA ++ +S + + L++K+ C LL + D
Sbjct: 94 HQDMQKAAAFVKRLATFALCFGCAESMSALVTLKTLLQKNVKCRNLLEN----DAGGGSV 149
Query: 417 QEYLDPFNNEETNPELTNALSSSVWELETLMNHYHPNVATLA 458
+ + T+P L+ AL++ +WEL L HYHP ++T+A
Sbjct: 150 SGSIAKYQPYATDPNLSGALATVLWELSLLSKHYHPAISTMA 191
>Hs20806097 [JU] KOG2153 Protein involved in the nuclear export of
pre-ribosomes
Length = 800
Score = 50.1 bits (118), Expect = 8e-06
Identities = 42/147 (28%), Positives = 66/147 (44%), Gaps = 18/147 (12%)
Query: 318 NLEYPNFYSKLYQLLTPEVMHVKYRSRFFRLV----DTFLSSTH--LSAQLVASFIKRLA 371
N++ FY+ LY+ L +H + +V D L+ +S Q +FIKRL
Sbjct: 574 NIDPLKFYTHLYKTLFK--LHAGATNEGVEIVLQCLDVMLTKRRKQVSQQRALAFIKRLC 631
Query: 372 RLSVSAPPGAIVSIIPFVYNLIRKHPTCMILLHDPEYIDCDSKKRQEYLDPFNNEETNPE 431
L++ P + + I+ L+ P +LL D +S+ +L E PE
Sbjct: 632 TLALHVLPNSSIGILATTRILMHTFPKTDLLL------DSESQGSGVFLP----ELDEPE 681
Query: 432 LTNALSSSVWELETLMNHYHPNVATLA 458
NA ++++WEL L HYHP V A
Sbjct: 682 YCNAQNTALWELHALRRHYHPIVQRFA 708
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,924,930
Number of Sequences: 60738
Number of extensions: 1266705
Number of successful extensions: 3073
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3049
Number of HSP's gapped (non-prelim): 11
length of query: 521
length of database: 30,389,216
effective HSP length: 111
effective length of query: 410
effective length of database: 23,647,298
effective search space: 9695392180
effective search space used: 9695392180
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)