ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2899 good C KOG1563 Energy production and conversion Mitochondrial protein Surfeit 1/SURF1/SHY1, required for expression of cytochrome oxidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2899 1002427 1001318 -370
(370 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR112w [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 r... 404 e-113
SPBC1215.01 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SH... 116 6e-26
7295311 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 r... 95 2e-19
Hs4507319 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1... 90 5e-18
CE23794 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 r... 90 5e-18
>YGR112w [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 required for
expression of cytochrome oxidase
Length = 389
Score = 404 bits (1039), Expect = e-113
Identities = 195/352 (55%), Positives = 247/352 (69%), Gaps = 4/352 (1%)
Query: 22 YSTVRTSTVDWKPIKTKKTPTEGFQKKESRWGKRIFLGLMIAMPVISFYLGTWQLRRLKW 81
Y + TSTVDWKPIKT K+P + ++E +GK+I LGLM AMP+ISFYLGTWQ+RRLKW
Sbjct: 39 YRGIITSTVDWKPIKTGKSPNDD-SRRERSFGKKIVLGLMFAMPIISFYLGTWQVRRLKW 97
Query: 82 KTNLIASCEDKLTYDPIPLPKTFTPDMCEDWEYRKCTMTGRFLHDEEIFVGPRVRGGIKG 141
KT LIA+CE KLTY+PIPLPK+FTPDMCEDWEYRK +TG FLH+EE+FVGPR + G KG
Sbjct: 98 KTKLIAACETKLTYEPIPLPKSFTPDMCEDWEYRKVILTGHFLHNEEMFVGPRKKNGEKG 157
Query: 142 YVLYTPFIRKDTGERILIERGWISEEKIVPSSRNLQHLSLPQGDNVTITVLVRAAKDLTS 201
Y L+TPFIR DTGE++LIERGWISEEK+ P SRNL HLSLPQ +++ + LVR K S
Sbjct: 158 YFLFTPFIRDDTGEKVLIERGWISEEKVAPDSRNLHHLSLPQEEHLKVVCLVRPPKKRGS 217
Query: 202 MQWKKEDRESRLWQVLDIKDMTEMTHTSPIHYQALYDLKDHH-WDEESKSEGIDNKNQSS 260
+QW K+D SRLWQV DI DM + +PI +QALYD+KDH +E +++E N + SS
Sbjct: 218 LQWAKKDPNSRLWQVPDIYDMARSSGCTPIQFQALYDMKDHPIIEEHTRNEASQNNSTSS 277
Query: 261 -WKFWXXXXXXXXXXXXXXXXXHVNTKKVNTADSETGLEFNEFQFVKAGVPIGKAPKIDL 319
WKFW + ++ +EF+E QF+KAGVPIG+ P IDL
Sbjct: 278 LWKFWKREPTTAVNGTQAVDNNTSKPRSRQEMPTDQTIEFDERQFIKAGVPIGRKPTIDL 337
Query: 320 KNNHLQYLITWYGXXXXXXXXXXXXXRRG-RGSAMSQDEIKRQKLKHAKKFM 370
KNNHLQYL+TWYG R+ RG +SQD++ ++KLKH++K+M
Sbjct: 338 KNNHLQYLVTWYGLSFLSTIFLIVALRKAKRGGVVSQDQLMKEKLKHSRKYM 389
>SPBC1215.01 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 required for
expression of cytochrome oxidase
Length = 290
Score = 116 bits (290), Expect = 6e-26
Identities = 82/232 (35%), Positives = 119/232 (50%), Gaps = 25/232 (10%)
Query: 1 MFRFIVARPFTVPKVRP-VRRLYSTVRTSTVDWKPIKTKKTPTEGFQKKESRWGKRIFLG 59
MF + A FT K P V R ST+ +TV +P K K +G
Sbjct: 1 MFWWKSATKFTFSKRGPCVFRYLSTLEGTTV--RPKKNK-----------------FLVG 41
Query: 60 LMIAMPVISFYLGTWQLRRLKWKTNLIASCEDKLTYDPIPLPKTFTPDMCEDWEYRKCTM 119
L+ A+P+++F LGTWQ++R +WK +I + ++L I LPKT T + E+ + +
Sbjct: 42 LLSAVPIVTFALGTWQVKRREWKMGIINTLTERLQQPAILLPKTVTEQDTKKLEWTRVLL 101
Query: 120 TGRFLHDEEIFVGPRVRGGIKGYVLYTPFIRKDTGERILIERGWISEEKIVPSSRNLQHL 179
G F HD+E+ VGPR + G GY + TPFI D G RIL+ RGWI+ SSR+
Sbjct: 102 RGVFCHDQEMLVGPRTKEGQPGYHVVTPFI-LDDGRRILVNRGWIARSFAEQSSRDPS-- 158
Query: 180 SLPQGDNVTITVLVRAAKDLTSMQWKKEDRESRLWQVLDIKDMTEMTHTSPI 231
SLP+G V I L+R D K E E + L++++ ++ T PI
Sbjct: 159 SLPKGP-VVIEGLLRQHTDKPRFMMKNEP-EKNSFYFLNVREFAQLKGTLPI 208
>7295311 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 required for
expression of cytochrome oxidase
Length = 293
Score = 94.7 bits (234), Expect = 2e-19
Identities = 69/232 (29%), Positives = 110/232 (46%), Gaps = 34/232 (14%)
Query: 24 TVRTSTVDWKPIKTKKTPTEGFQKKESRWGKRIFLG-LMIAMPVISFYLGTWQLRRLKWK 82
T + V+W T P + + KE K LG ++ +P +F LG WQ++R WK
Sbjct: 30 TQQRPPVNW----TTSIPNQAAKDKE----KIAPLGWFLLLIPATTFGLGCWQVKRKIWK 81
Query: 83 TNLIASCEDKLTYDPIPLPKTFTPDMCEDWEYRKCTMTGRFLHDEEIFVGPR-------- 134
LI +L+ P+ LP T D+ + EYR + GRFLHD+E+ +GPR
Sbjct: 82 EQLIKDLNKQLSTAPVALPDDLT-DLAQ-MEYRLVKIRGRFLHDKEMRLGPRSLIRPDGV 139
Query: 135 -VRGGI-------KGYVLYTPFIRKDTGERILIERGWISEEKIVPSSRNLQHLSLPQGDN 186
+GG+ GY++ TPF D + +L+ RGW+S +++ P +R L Q
Sbjct: 140 ETQGGLFSQRDSGNGYLIVTPFQLADRDDIVLVNRGWVSRKQVEPETRPLGQ----QQAE 195
Query: 187 VTITVLVRAAKDLTSMQWKKEDRESRLWQVLDIKDMTEMTHTSPIHYQALYD 238
V +T +VR + D + ++ D+ M T +P+ A+YD
Sbjct: 196 VELTAVVRKGEARPQF---TPDHKGNVYLYRDLARMCAATGAAPVFLDAVYD 244
>Hs4507319 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 required for
expression of cytochrome oxidase
Length = 300
Score = 90.1 bits (222), Expect = 5e-18
Identities = 64/222 (28%), Positives = 102/222 (45%), Gaps = 26/222 (11%)
Query: 30 VDWKPIKTKKTPTEGFQKKESRWGKRIFLGLMIAMPVISFYLGTWQLRRLKWKTNLIASC 89
V W+P + + E K +++ +PV +F LGTWQ++R KWK NLIA
Sbjct: 37 VAWRPSRCGSSAAEASATKAE--DDSFLQWVLLLIPVTAFGLGTWQVQRRKWKLNLIAEL 94
Query: 90 EDKLTYDPIPLPKTFTPDMCEDWEYRKCTMTGRFLHDEEIFVGPRV---------RGGI- 139
E ++ +P+PLP P ++ EYR + G F H +E+++ PR GG+
Sbjct: 95 ESRVLAEPVPLPA--DPMELKNLEYRPVKVRGCFDHSKELYMMPRTMVDPVREAREGGLI 152
Query: 140 -----KGYVLYTPFIRKDTGERILIERGWISEEKIVPSSRNLQHLSLPQGDNVTITVLVR 194
G + TPF D G IL+ RG++ +K+ P +R + V + +VR
Sbjct: 153 SSSTQSGAYVVTPFHCTDLGVTILVNRGFVPRKKVNPETRQKGQIE----GEVDLIGMVR 208
Query: 195 AAKDLTSMQWKKEDR-ESRLWQVLDIKDMTEMTHTSPIHYQA 235
+ T + E+ E W D++ M +T PI A
Sbjct: 209 LTE--TRQPFVPENNPERNHWHYRDLEAMARITGAEPIFIDA 248
>CE23794 [C] KOG1563 Mitochondrial protein Surfeit 1/SURF1/SHY1 required for
expression of cytochrome oxidase
Length = 323
Score = 90.1 bits (222), Expect = 5e-18
Identities = 64/211 (30%), Positives = 97/211 (45%), Gaps = 26/211 (12%)
Query: 47 KKESRWGKRIFLGLMIAMPVISFYLGTWQLRRLKWKTNLIASCEDKLTYDPIPLPKTFTP 106
KK +W L M+ +PV +F LG WQ RLKWK +LI + +L LP+ +
Sbjct: 76 KKSIKWSTGSVL--MLTIPVFAFSLGIWQTFRLKWKLDLIEHLKGRLNQTAQELPEDLSC 133
Query: 107 DMCEDWEYRKCTMTGRFLHDEEIFVGPRVR-----------GGI--------KGYVLYTP 147
+ E EY + T+TG FLH++E + PR R G + G L TP
Sbjct: 134 ESLEPLEYCRVTVTGEFLHEKEFIISPRGRFDPGKKTSAAAGSMLSENEMSSHGGHLITP 193
Query: 148 FIRKDTGERILIERGWISEEKIVPSSRNLQHLSLPQGDNVTITVLVRAAKDLTSMQWKKE 207
F K++G+ ILI RGW+ P +R + P+G +T+ +VR + +
Sbjct: 194 FRLKNSGKIILINRGWLPSFYFDPETRQKTN---PRG-TLTLPAIVRKTEKRPQFVGQNV 249
Query: 208 DRESRLWQVLDIKDMTEMTHTSPIHYQALYD 238
E +W D+ M + T P+ A Y+
Sbjct: 250 P-EQGVWYYRDLNQMAKHYGTEPVLLDAAYE 279
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,936,318
Number of Sequences: 60738
Number of extensions: 916797
Number of successful extensions: 1893
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1873
Number of HSP's gapped (non-prelim): 9
length of query: 370
length of database: 30,389,216
effective HSP length: 108
effective length of query: 262
effective length of database: 23,829,512
effective search space: 6243332144
effective search space used: 6243332144
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)