ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII2963 good H KOG1276 Coenzyme transport and metabolism Protoporphyrinogen oxidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII2963 1020305 1018698 -536
(536 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER014w [H] KOG1276 Protoporphyrinogen oxidase 470 e-132
SPAC1F5.07c [H] KOG1276 Protoporphyrinogen oxidase 173 5e-43
7301191 [H] KOG1276 Protoporphyrinogen oxidase 122 1e-27
Hs4506001 [H] KOG1276 Protoporphyrinogen oxidase 111 3e-24
At4g01690 [H] KOG1276 Protoporphyrinogen oxidase 83 9e-16
At5g14220 [H] KOG1276 Protoporphyrinogen oxidase 77 5e-14
>YER014w [H] KOG1276 Protoporphyrinogen oxidase
Length = 539
Score = 470 bits (1210), Expect = e-132
Identities = 257/531 (48%), Positives = 346/531 (64%), Gaps = 11/531 (2%)
Query: 12 ARIAVVGGGVSGLTFTYFLSKLRPDVKVTLYEKQDRCGGWIHSDIIDDGEGNRVMVEKGP 71
A++AVVGGGVSGL FTYFLSKLRPDV++TL+E Q+R GGWI+S D GN +M+EKGP
Sbjct: 12 AKVAVVGGGVSGLCFTYFLSKLRPDVEITLFESQNRTGGWIYSCNTRDMSGNPIMLEKGP 71
Query: 72 RTLRGVSDGSVIIVQTLRDLGKQDVVQYIAADSEANRKFLLDKNNVLTQVPDSPISALKF 131
RTLRGVSDG+V+I+ TL+DLGK+ V+Q I A++KFLLD ++ L QVP+S + +KF
Sbjct: 72 RTLRGVSDGTVLIMDTLKDLGKEAVIQSIDKGCIADKKFLLDPSDKLVQVPNSISTTVKF 131
Query: 132 FAGSLGKGFISGILGEPFR-SAKVTGTDESAHSFIHRRFGNEHISKNVFSALFHGIYAGD 190
LGKG I+G++GE FR + G DES S RRFGN +IS N+ SAL GIY D
Sbjct: 132 LLNPLGKGLITGMMGEWFRKKSPHPGQDESVESICDRRFGNNYISNNMISALLRGIYGDD 191
Query: 191 IKKLSAKRTIGKMVEMENTSGSLIKSMFNKMIIKYKNRNDTRSHKIEISPLLKKFAEATG 250
+ LSAKRT K+ E GS ++M + M K +++ H+ ++ L ++ A G
Sbjct: 192 VSLLSAKRTFKKIYYNELKHGSNTQAMIDNMRGKSRSKKTENLHQ-SLTGCLNDYSNAFG 250
Query: 251 QSEKQLISLSQRLKAFPMVGFRSGLDCYPKTLSEYITGLPNVS-VKHEAVESVIPGKDGK 309
+ +L+ LS LK +PM+G GL+ +PK + + NV V V ++ +
Sbjct: 251 KDRSKLLDLSNTLKKYPMLGLAGGLETFPKIVRNALNEFKNVKIVTGNPVTQIMKRPANE 310
Query: 310 NVV-IVSDSGE--STFDHARLTVNPNVLENIIPFE--PLAKKLREVHSNTVILVNFYLPR 364
+ + + SG+ TFDH RLT+ P + ++P + L+K L E+ SNT+ILVN+YLP
Sbjct: 311 TTIGLKAKSGDQYETFDHLRLTITPPKIAKLLPKDQNSLSKLLDEIQSNTIILVNYYLPN 370
Query: 365 KDVI-SNYHGFGYLVPQSNQNHEKLLGVIFDSVIEKNFKPVFGVQNESKSPDDDKRYTKV 423
KDVI ++ GFGYLVP+SN+N KLLGVIFDSVIE+NFKP+F S +P+ +YTKV
Sbjct: 371 KDVIDADLQGFGYLVPKSNKNPGKLLGVIFDSVIERNFKPLF--DKLSTNPNALNKYTKV 428
Query: 424 TAMLGGHYLNNEGEQNIPSKSIIINSVKYAFMKHLHIDENDLEDGTWEVTVAKDCLPQYH 483
TAM+GG LN G +PS+ + IN+VK A HL I DLE G WE T+A CLP++H
Sbjct: 429 TAMIGGCMLNEHGVPVVPSREVTINAVKDALNNHLGISNKDLEAGQWEFTIADRCLPRFH 488
Query: 484 VGYDDWVQQTAKLVQEQYSNYVSLGGMAFSTGPGVPNVIEDGFESAYVLSK 534
VGYD W ++ + +QE Y VS+GGM FS PGVP+VI DGF A LSK
Sbjct: 489 VGYDAWQERAERKLQESYGQTVSVGGMGFSRSPGVPDVIVDGFNDALQLSK 539
>SPAC1F5.07c [H] KOG1276 Protoporphyrinogen oxidase
Length = 490
Score = 173 bits (439), Expect = 5e-43
Identities = 135/527 (25%), Positives = 252/527 (47%), Gaps = 53/527 (10%)
Query: 14 IAVVGGGVSGLTFTYFLSKLRPDVKVTLYEKQDRCGGWIHSDIID--DGEGNRVMVEKGP 71
IA+ GGG++GL+ ++L++L P + LYEK R GGW+ S I D V+ E+GP
Sbjct: 3 IAICGGGIAGLSTAFYLARLIPKCTIDLYEKGPRLGGWLQSVKIPCADSPTGTVLFEQGP 62
Query: 72 RTLRGVSDGSVIIVQTLRDLGKQDVVQYIAADSEANRKFLLDKNNVLTQVPDSPISALKF 131
RTLR + + + LG +D + I+++S + + + + L ++P S + ++K
Sbjct: 63 RTLRPAGVAGLANLDLISKLGIEDKLLRISSNSPSAKNRYIYYPDRLNEIPSSILGSIKS 122
Query: 132 FAGSLGKGFISGILGEPFRSAKVTGTDESAHSFIHRRFGNEHISKNVFSALFHGIYAGDI 191
+ ++ EPFR +K TDES SF+ RRFG ++++ V SA+ +GIYAGD+
Sbjct: 123 IMQPALRPMPLAMMLEPFRKSKRDSTDESVGSFMRRRFG-KNVTDRVMSAMINGIYAGDL 181
Query: 192 KKLSAKRTI-GKMVEMENTSGSLIKSMFNKMIIKYKNRNDTRSHKIEISPLLKKFAEATG 250
LS ++ G + ++E G++ + ++ + EI +K +A
Sbjct: 182 NDLSMHSSMFGFLAKIEKKYGNITLGLIRALLAR------------EILSPAEKALKAAL 229
Query: 251 QSEKQLISLSQRLKAFPMVGFRSGLDCYPKTLSEYITGLPNVSVK-HEAVESVIPGKDGK 309
+E + LS +K+ M F+ G++ ++++ + +PNV + ++ ++++P K
Sbjct: 230 LAEPKTAELSNSMKSTSMFAFKEGIETITLSIADELKKMPNVKIHLNKPAKTLVPHKTQS 289
Query: 310 NVVIVSDSGESTFDHARLTVNPNVLENIIPFEPLAKKLREVHSNTVILVNFYLPRKDVIS 369
V D +++ + LEN+I + E +++V +VN Y +V+
Sbjct: 290 LV----DVNGQAYEYVVFANSSRNLENLISCPKM-----ETPTSSVYVVNVYYKDPNVLP 340
Query: 370 NYHGFGYLVPQ---SNQNHEKLLGVIFDSVIEKNFKPVFGVQNESKSPDDDKRYTKVTAM 426
GFG L+P +N NH +LG++FDS E +P++ +KVT M
Sbjct: 341 -IRGFGLLIPSCTPNNPNH--VLGIVFDS--------------EQNNPENG---SKVTVM 380
Query: 427 LGGHYLNNEGEQNIPSKSIIINSVKYAFMKHLHIDENDLEDGTWEVTVAKDCLPQYHVGY 486
+GG + +N+ A L I + T+ ++C+PQY VG+
Sbjct: 381 MGGSAYTKNTSLIPTNPEEAVNNALKALQHTLKISS---KPTLTNATLQQNCIPQYRVGH 437
Query: 487 DDWVQQTAKLVQEQYSNYVSLGGMAFSTGPGVPNVIEDGFESAYVLS 533
D + +++ + L G ++ G + + I +G +A L+
Sbjct: 438 QDNLNSLKSWIEKNMGGRILLTG-SWYNGVSIGDCIMNGHSTARKLA 483
>7301191 [H] KOG1276 Protoporphyrinogen oxidase
Length = 475
Score = 122 bits (306), Expect = 1e-27
Identities = 143/520 (27%), Positives = 225/520 (42%), Gaps = 73/520 (14%)
Query: 15 AVVGGGVSGLTFTYFLSKLRPDVKVTLYEKQDRCGGWIHSDIIDDGEGNRVMVEKGPRTL 74
AV+GGG+SGL+ Y+L + R +T+YE R GGW+ S+ D + E GPRT+
Sbjct: 4 AVLGGGLSGLSAGYYLLR-RFGKPLTIYEASPRVGGWVRSENRKD---RNFIFESGPRTI 59
Query: 75 RGVSDGSVIIVQTLRDLGKQDVVQYIAADSEANRKFLLDKNNVLTQVPDSPISALKFFAG 134
R V + ++ + DL + V I A R +L L +P+SP L
Sbjct: 60 RPVGEPGANTLELVEDLKLE--VTPIRRSHVAARNRMLYAKGQLCMLPNSP-KGLFGVLP 116
Query: 135 SLGKGFISGILGEPFRSAKVTGT-DESAHSFIHRRFGNEHISKNVFSALFHGIYAGDIKK 193
K +L + F ++K DES +SF RRFG E I+ S + GI AGD ++
Sbjct: 117 PFTKPLYKAVLRDLFTASKKAKLEDESIYSFAERRFGKE-IADYAISPMICGICAGDARE 175
Query: 194 LSAKRTIGKMVEMENTSGSLIKSMFNKMIIKYKNRNDTRSHKIEISPLLKKFAEATGQSE 253
+S + + + E E G ++K +I +N T+ K + FAE +
Sbjct: 176 ISVRFLMEGLFEKEQKYGGVLKG----TLISRFEKNKTKDTKDGL------FAERQPKLY 225
Query: 254 KQLISLSQRLKAFPMVGFRSGLDCYPKTLSEYITGLPNVSVKHEAVESVIPGKDGKNVVI 313
Q + + + M G + GL+ PKT+ +Y+ G +V+V+ + +N+
Sbjct: 226 AQAVK-----EKWAMYGLKGGLENLPKTMRKYL-GERDVNVQ--------LSNECRNLTF 271
Query: 314 VSDSGESTFDHARLTVNPNVLENIIPFEPLAKKLREVHSN-----------TVILVNFYL 362
S A + V V + +P LA +++ H + V++VN
Sbjct: 272 SSSGVRMNIKDAEVPVEHVV--SSLPAYKLAPLVKQQHPSLSAQLLSIPYVDVLVVNMQF 329
Query: 363 PRKDVISNYHGFGYLVPQSNQNHEKLLGVIFDSVIEKNFKPVFGVQNESKSPDDDKRYTK 422
P K + GFG LVP + LLGVIFDS D T
Sbjct: 330 PGK--LLKQDGFGLLVPPVEK--LPLLGVIFDSCC-----------------FDMGENTV 368
Query: 423 VTAMLGGHYLNNEGEQNIPSKSIIINSVKYAFMKHLHIDENDLEDGTWEVTVAKDCLPQY 482
+T M+GGH+ ++ + PS I++ K L I E E V C+PQY
Sbjct: 369 LTVMMGGHWF-DQWFGDRPSPKQILDLATSHVQKMLQIRE---EPKFSRVHTLHKCIPQY 424
Query: 483 HVGYDDWVQQTAKLVQEQYSNYVSLGGMAFSTGPGVPNVI 522
VG+ V+ + + Y +S+ G A+ G G+ +VI
Sbjct: 425 TVGHKRRVEAIRNYI-KTYKLPLSVCGAAYD-GVGINDVI 462
>Hs4506001 [H] KOG1276 Protoporphyrinogen oxidase
Length = 477
Score = 111 bits (277), Expect = 3e-24
Identities = 130/522 (24%), Positives = 221/522 (41%), Gaps = 65/522 (12%)
Query: 14 IAVVGGGVSGLTFTYFLSKLRPDVKVTLYEKQDRCGGWIHSDIIDDGEGNRVMVEKGPRT 73
+ V+GGG+SGL +Y LS+ KV L E +R GGWI S +G + E GPR
Sbjct: 5 VVVLGGGISGLAASYHLSRAPCPPKVVLVESSERLGGWIRSVRGPNG----AIFELGPRG 60
Query: 74 LRGVSDGSVIIVQTLRDLGKQDVVQYIAADSEANRKFLLDKNNVLTQVPDSPISALKFFA 133
+R + + +LG V + D A + L L +P L+
Sbjct: 61 IRPAGALGARTLLLVSELGLDSEVLPVRGDHPAAQNRFLYVGGALHALPTGLRGLLRPSP 120
Query: 134 GSLGKGFISGILGEPFRSAKVTGTDESAHSFIHRRFGNEHISKNVFSALFHGIYAGDIKK 193
F +G+ + DE+ HSF RR G E ++ +L G++AG+ ++
Sbjct: 121 PFSKPLFWAGL--RELTKPRGKEPDETVHSFAQRRLGPE-VASLAMDSLCRGVFAGNSRE 177
Query: 194 LSAKRTIGKMVEMENTSGSLIKSMFNKMIIKYKNRNDTRSHKIEISPLLKKFAEATGQSE 253
LS + + + E T S++ + A T Q +
Sbjct: 178 LSIRSCFPSLFQAEQTHRSILLGLLLG-------------------------AGRTPQPD 212
Query: 254 KQLI--SLSQRLKAFPMVGFRSGLDCYPKTLSEYITGLPNVSVKHEAVESVIPGKDGKNV 311
LI +L++R + + R GL+ P+ L ++T ++ + V + +G+
Sbjct: 213 SALIRQALAERWSQWSL---RGGLEMLPQALETHLTSRGVSVLRGQPVCGLSLQAEGRWK 269
Query: 312 VIVSDSGESTFDHARLTVNPNVLENIIPFE--PLAKKLREVHSNTVILVNFYLPRKDVIS 369
V + DS DH + +VL ++P E PLA+ L + + +V +VN L +
Sbjct: 270 VSLRDSSLEA-DHVISAIPASVLSELLPAEAAPLARALSAITAVSVAVVN--LQYQGAHL 326
Query: 370 NYHGFGYLVPQSNQNHEKLLGVIFDSVIEKNFKPVFGVQNESKSPDDDKRYTKVTAMLGG 429
GFG+LVP S +LG+++DSV + SP +VT MLGG
Sbjct: 327 PVQGFGHLVPSSED--PGVLGIVYDSV---------AFPEQDGSPPG----LRVTVMLGG 371
Query: 430 HYLNN-EGEQNIPSKSIIINSVKYAFMKHLHIDENDLEDGTWEVTVAKDCLPQYHVGYDD 488
+L E + S+ + + A L + E V + K+C+PQY +G+
Sbjct: 372 SWLQTLEASGCVLSQELFQQRAQEAAATQLGLKEMPSH---CLVHLHKNCIPQYTLGH-- 426
Query: 489 WVQ-QTAKLVQEQYSNYVSLGGMAFSTGPGVPNVIEDGFESA 529
W + ++A+ + ++L G ++ G V + IE G ++A
Sbjct: 427 WQKLESARQFLTAHRLPLTLAGASYE-GVAVNDCIESGRQAA 467
>At4g01690 [H] KOG1276 Protoporphyrinogen oxidase
Length = 537
Score = 83.2 bits (204), Expect = 9e-16
Identities = 140/545 (25%), Positives = 213/545 (38%), Gaps = 92/545 (16%)
Query: 6 GSLPFNARIAVVGGGVSGLTFTYFLSKLRPDVKVTLY--EKQDRCGGWIHSDIIDDGEGN 63
G +VGGG+SGL L+ PD L E +DR GG I + E N
Sbjct: 51 GGTTITTDCVIVGGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITR-----EEN 105
Query: 64 RVMVEKGPRTLRGVSDGSVIIVQTLRDLGKQDVVQYIAADSEANRKFLLDKNNVLTQVP- 122
+ E+GP + + ++V D G +D + D A R L N L VP
Sbjct: 106 GFLWEEGPNSFQPSDPMLTMVV----DSGLKD--DLVLGDPTAPRFVLW--NGKLRPVPS 157
Query: 123 ---DSPISALKFFAGSLGKGFIS-GILGEPFRSAKVTGTDESAHSFIHRRFGNEHISKNV 178
D P L G + GF + GI P G +ES F+ R G+E + + +
Sbjct: 158 KLTDLPFFDLMSIGGKIRAGFGALGIRPSP------PGREESVEEFVRRNLGDE-VFERL 210
Query: 179 FSALFHGIYAGDIKKLSAKRTIGKMVEMENTSGSLIKSMFNKMIIKYKNRNDTRSHKIEI 238
G+YAGD KLS K GK+ ++E GS+I F K I + KN + K E
Sbjct: 211 IEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTF-KAIQERKN-----APKAER 264
Query: 239 SPLLKKFAEATGQSEKQLISLSQRLKAFPMVGFRSGLDCYPKTLSEYITGLPNVSVKHEA 298
P L K GQ+ + FR GL P+ +S + +S K
Sbjct: 265 DPRLPK---PQGQT---------------VGSFRKGLRMLPEAISARLGSKVKLSWKLSG 306
Query: 299 VESVIPGKDGKNVVIVSDSGESTFDHAR--LTVNPNVLENIIP--FEPLAKKLREVHSNT 354
+ + G G N+ + G + +TV +V ++ E A L +++
Sbjct: 307 ITKLESG--GYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLSESAANALSKLYYPP 364
Query: 355 VILVNFYLPRKDVIS------NYHGFGYLVPQSNQNHEKLLGVIFDSVIEKNFKPVFGVQ 408
V V+ P++ + + GFG L P++ + LG I+ S + N P
Sbjct: 365 VAAVSISYPKEAIRTECLIDGELKGFGQLHPRT--QGVETLGTIYSSSLFPNRAP----- 417
Query: 409 NESKSPDDDKRYTKVTAMLGGHYLNNEGEQNIPSKS--IIINSVKYAFMKHLHIDENDLE 466
+L +Y+ I SKS ++ +V K L I N +
Sbjct: 418 --------------PGRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKML-IKPNSTD 462
Query: 467 DGTWEVTVAKDCLPQYHVGYDDWVQQTAK--LVQEQYSNYVSLGGMAFSTGPGVPNVIED 524
V V +PQ+ VG+ D + TAK L Y + LGG + G + +E
Sbjct: 463 PLKLGVRVWPQAIPQFLVGHFD-ILDTAKSSLTSSGYEG-LFLGG-NYVAGVALGRCVEG 519
Query: 525 GFESA 529
+E+A
Sbjct: 520 AYETA 524
>At5g14220 [H] KOG1276 Protoporphyrinogen oxidase
Length = 504
Score = 77.4 bits (189), Expect = 5e-14
Identities = 105/421 (24%), Positives = 167/421 (38%), Gaps = 87/421 (20%)
Query: 13 RIAVVGGGVSGLTFTYFLSKLRPDVKVTLYEKQDRCGGWIHSDIIDDG----EGNRVMVE 68
R+AVVG GVSGL Y L + VT++E R GG + S ++ +G EG M E
Sbjct: 18 RVAVVGAGVSGLAAAYKLKS--RGLNVTVFEADGRVGGKLRS-VMQNGLIWDEGANTMTE 74
Query: 69 KGPRTLRGVSDGSVIIVQTLRDLGKQDVVQYIAADSEANRKFLLDKNNVLTQVPDSPISA 128
P GS+ L DLG ++ Q+ + +K + +N V +P +PI
Sbjct: 75 AEPEV------GSL-----LDDLGLREKQQF----PISQKKRYIVRNGVPVMLPTNPIEL 119
Query: 129 LKFFAGSLGKGFISGILGEPF----RSAKVT--GTDESAHSFIHRRFGNEHISKNVFSAL 182
+ S F IL EPF +S+KV+ +ES F R FG E + +
Sbjct: 120 VTSSVLSTQSKF--QILLEPFLWKKKSSKVSDASAEESVSEFFQRHFGQE-VVDYLIDPF 176
Query: 183 FHGIYAGDIKKLSAKRTIGKMVEMENTSGSLIKSMFNKMIIKYKNRNDTRSHKIEISPLL 242
G A D LS K + + +E + GS+I + +
Sbjct: 177 VGGTSAADPDSLSMKHSFPDLWNVEKSFGSII-----------------------VGAIR 213
Query: 243 KKFAEATGQSEKQLISLSQRLKAFPMVGFRSGLDCYPKTLSEYITGLPNVSVKHEAVESV 302
KFA G+S S + + F+ G+ P TL + S+ H+ +
Sbjct: 214 TKFAAKGGKSRDTKSSPGTKKGSRGSFSFKGGMQILPDTLCK--------SLSHDEI--- 262
Query: 303 IPGKDGKNVVIVSDSGESTFDHARLTVNPNVLENIIPFEPLA-----KKLREVHSNTVIL 357
D K + + +SG + + V+ N + P A K+++ +
Sbjct: 263 --NLDSKVLSLSYNSGSRQENWSLSCVSHNETQRQNPHYDAAPLCNVKEMKVMKGGQPFQ 320
Query: 358 VNF-----YLPRKDVISNY---------HGFGYLVPQSNQNHE-KLLGVIFDSVIEKNFK 402
+NF Y+P +I+ + GFG L+P Q H K LG +F S++ +
Sbjct: 321 LNFLPEINYMPLSVLITTFTKEKVKRPLEGFGVLIPSKEQKHGFKTLGTLFSSMMFPDRS 380
Query: 403 P 403
P
Sbjct: 381 P 381
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,024,084
Number of Sequences: 60738
Number of extensions: 1480874
Number of successful extensions: 3657
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3636
Number of HSP's gapped (non-prelim): 7
length of query: 536
length of database: 30,389,216
effective HSP length: 111
effective length of query: 425
effective length of database: 23,647,298
effective search space: 10050101650
effective search space used: 10050101650
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)