ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3171 good H KOG2525 Coenzyme transport and metabolism Folylpolyglutamate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3171 1091010 1089745 -422
(422 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR113w [H] KOG2525 Folylpolyglutamate synthase 468 e-132
YKL132c [H] KOG2525 Folylpolyglutamate synthase 275 7e-74
SPAC227.09 [H] KOG2525 Folylpolyglutamate synthase 265 1e-70
At5g05980 [H] KOG2525 Folylpolyglutamate synthase 131 2e-30
CE29283 [H] KOG2525 Folylpolyglutamate synthase 129 6e-30
CE01923 [H] KOG2525 Folylpolyglutamate synthase 129 6e-30
HsM4826728 [H] KOG2525 Folylpolyglutamate synthase 129 1e-29
At5g41480 [H] KOG2525 Folylpolyglutamate synthase 127 2e-29
At3g55630 [H] KOG2525 Folylpolyglutamate synthase 127 2e-29
YOR241w [H] KOG2525 Folylpolyglutamate synthase 121 2e-27
SPBC1709.17 [H] KOG2525 Folylpolyglutamate synthase 120 3e-27
7292786 [H] KOG2525 Folylpolyglutamate synthase 112 8e-25
7295749_2 [H] KOG2525 Folylpolyglutamate synthase 87 5e-17
At3g10160 [H] KOG2525 Folylpolyglutamate synthase 84 5e-16
>YMR113w [H] KOG2525 Folylpolyglutamate synthase
Length = 427
Score = 468 bits (1204), Expect = e-132
Identities = 229/429 (53%), Positives = 310/429 (71%), Gaps = 10/429 (2%)
Query: 1 MPIDLGLLRLTQLLSHLGNPHLNLKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPH 60
M I+LGL R+T+LL HLGNP +L+VLHIAGTNGKGSVC+YL+SVLQQ +Y+ GKFT+PH
Sbjct: 1 MAIELGLSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPH 60
Query: 61 LCHVRDSITVNQVPISSHDFTTIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWC 120
L HV DSIT+N PI + I+ QLE L++ +SLKCTEFEL+T TA YF V+C WC
Sbjct: 61 LVHVTDSITINNKPIPLERYQNIRLQLEALNKSHSLKCTEFELLTCTAFKYFYDVQCQWC 120
Query: 121 ILEVGLGGRLDATNVIPGKNKV-CGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVA 179
++EVGLGGRLDATNVIPG NK CGITKI LDHESFLGN L I+ EKAGI+ EG F
Sbjct: 121 VIEVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEGVPFTV 180
Query: 180 VDGTNDDAVVRVVEDKARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNV 239
+DGTN+ +V+ VV+++ +++G+E+ T+ + + T+SWG DL++LPLNGEYQ+ N+
Sbjct: 181 IDGTNEASVINVVKERCKALGSELSVTDSQLNGNMIDTNSWGCFDLAKLPLNGEYQIFNL 240
Query: 240 QVAIQILDYMQRTGMIQLDSNQILDGIQTAEWPGRLQFINYHYS--PRKTTPVLFDGAHN 297
+VA+ +LDY+Q +I + N++ + +WPGRL ++Y + +T P+L DGAHN
Sbjct: 241 RVAMGMLDYLQMNELIDITKNEVSTRLAKVDWPGRLYRMDYRFDKVSNRTVPILMDGAHN 300
Query: 298 GSAAVELAKYLDTEFRFSKDEPLTFVIAITNGKTLEPLLSPIIRPQDKVICTEFGSVEGM 357
GSAAVEL KYL E+ ++PLTFV+A+T+GK LEPLL P++RP D+VI T F +VEGM
Sbjct: 301 GSAAVELVKYLRKEY---GNQPLTFVMAVTHGKNLEPLLQPLLRPIDQVILTRFNNVEGM 357
Query: 358 PWIKPMDAYDLASQV--GHFTKNVEVNRDLFDILPALAQSGPETK--VVVCGSLYLVGQL 413
PWI D ++ + +TK + + DL +LP+LA E + +VVCGSLYL G+L
Sbjct: 358 PWIHATDPEEIKDFILTQGYTKEIVIENDLHQVLPSLAHVSDEQRRPIVVCGSLYLCGEL 417
Query: 414 LNVHYDNVR 422
L +H ++R
Sbjct: 418 LRIHNSHLR 426
>YKL132c [H] KOG2525 Folylpolyglutamate synthase
Length = 430
Score = 275 bits (704), Expect = 7e-74
Identities = 156/423 (36%), Positives = 243/423 (56%), Gaps = 20/423 (4%)
Query: 7 LLRLTQLLSHLGNPHLNLKVLHIAGTNGKGSVCSYLNSVLQQSTYKT-----GKFTSPHL 61
L R+ +LL H+GNP +L +LHIAGTNGK +V +L S+LQ + G++T+ L
Sbjct: 10 LPRINRLLEHVGNPQDSLSILHIAGTNGKETVSKFLTSILQHPGQQRQRVLIGRYTTSSL 69
Query: 62 CHVRDS-ITVNQVPISSHDFTTIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWC 120
+ ++ I++N IS +++ I+ +L + D L+C EL+T+ AL YF + C WC
Sbjct: 70 LNAKEEDISINNEAISLIEYSRIEKELIEADSSLKLQCNNLELLTSVALVYFAKKNCQWC 129
Query: 121 ILEVGLGGRLDATNVIPGKNKVC-GITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVA 179
I+E GL G+ D ++I G+++VC IT +G+ E+FL L I +
Sbjct: 130 IIETGLAGKQDPGSIIAGQSRVCCAITNVGISDEAFLCKFLSQITESSTNKAI-----FL 184
Query: 180 VDGTNDDAVVRVVEDKARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNV 239
+DG+ND+ V + + VG + T+P +V T +WG +++ EYQ+ N+
Sbjct: 185 LDGSNDEFVRNTITKRCHDVGCPLEITDPSLRDYNVHTDTWGTLEVRLPYSEEEYQIFNL 244
Query: 240 QVAIQILDYMQRTGMIQLDSNQILDGIQTAEWPGRLQFINYHYSPR--KTTPVLFDGAHN 297
+VAI +LD++ + + + +Q+ G+ + +WP L ++Y Y K +L D A+N
Sbjct: 245 RVAIAVLDFLSKEKKVCISKDQLSQGLISVDWPRSLHRLDYCYESTSGKKIALLLDNANN 304
Query: 298 GSAAVELAKYLDTEFRFSKDEPLTFVIAITNGKTLEPLLSPIIRPQDKVICTEFGSVEGM 357
AA LA +L T + D PLTFVIAIT GK + PLL P+IRPQD VI T FGSV GM
Sbjct: 305 AKAARNLACHLRTTYG---DTPLTFVIAITTGKKVSPLLDPLIRPQDYVIVTRFGSVVGM 361
Query: 358 PWIKPMDAYDLASQV-GHFTKNVEVNRDLFDILPALAQSGPET--KVVVCGSLYLVGQLL 414
PWI+ ++ +L + + +T+NV + DL + L SG +T V+VCGSLY+ +LL
Sbjct: 362 PWIQSLEPVNLLAFIKNRYTRNVNMQPDLQSVWTFLETSGLKTIVPVIVCGSLYICKELL 421
Query: 415 NVH 417
+H
Sbjct: 422 RLH 424
>SPAC227.09 [H] KOG2525 Folylpolyglutamate synthase
Length = 417
Score = 265 bits (676), Expect = 1e-70
Identities = 164/422 (38%), Positives = 241/422 (56%), Gaps = 22/422 (5%)
Query: 1 MPIDLGLLRLTQLLSHLGNPHLNLKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPH 60
MPI LGL R+ QLL HLGNP + + IAGTNGKGS+CSY+ + L Q+ KTG++TSPH
Sbjct: 1 MPIQLGLQRMLQLLKHLGNPQESFCAVQIAGTNGKGSICSYIYTSLLQAAIKTGRYTSPH 60
Query: 61 LCHVRDSITVNQVPISSHDFTTIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWC 120
RD+I++N S F T Q+ ++D R+ K TEFEL+TATA F+
Sbjct: 61 FLEPRDTISINGQIASEEIFNTCWKQVIEVDRRFRTKATEFELLTATAFQCFHHSGVRVA 120
Query: 121 ILEVGLGGRLDATNVIPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAV 180
++E G+GGRLDATNV + + I++I LDH++FLGN L +IA EKAGI + V V
Sbjct: 121 VIETGMGGRLDATNVFE-EPVLSIISRICLDHQAFLGNTLEAIAKEKAGIFKKNVPCV-V 178
Query: 181 DGTNDDAVVRVVEDKARSVGAEIV-----KTNPDTSIKSVVTSSWGPIDLSELPLNGEYQ 235
DG N+ V+ ++ A A K+ + + + T +WG S PL G+YQ
Sbjct: 179 DGLNEVNVLNQLKLSAEETRAHPFYLAKGKSGENKNEWIINTPNWGTNTFS-TPLKGDYQ 237
Query: 236 VTNVQVAIQILDYMQRTGMIQLDSNQILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGA 295
N+ A+ LD + + I L + +G++ WPGRL S +LFDGA
Sbjct: 238 GQNLACAVTALDILSSSFSIMLP--HVQNGVKNTSWPGRLDI----RSVPSLGDILFDGA 291
Query: 296 HNGSAAVELAKYLDTEFRFSKDEPLTFVIAITNGKTLEPLLSPIIRPQDKVICTEFGSVE 355
HN AA+ELAK+++++ R ++ +++V+A TN K + ++ ++R D VI T F SV
Sbjct: 292 HNKEAAIELAKFVNSQ-RREHNKSVSWVVAFTNTKDVTGIMKILLRKGDTVIATNFSSVS 350
Query: 356 GMPWIKPMDAYDLASQVGHFTKNVEV----NRDLFDILPALAQSGPETKVVVCGSLYLVG 411
GMPWIK M+ + + + +VE N + +IL + + V+VCGSLYL+G
Sbjct: 351 GMPWIKSMEPEVIKNSISS-ESSVECYTADNLTISEILRLAKEK--NSSVIVCGSLYLLG 407
Query: 412 QL 413
+
Sbjct: 408 DM 409
>At5g05980 [H] KOG2525 Folylpolyglutamate synthase
Length = 566
Score = 131 bits (329), Expect = 2e-30
Identities = 96/304 (31%), Positives = 147/304 (47%), Gaps = 31/304 (10%)
Query: 22 LNLKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISSHDFT 81
L + V+H+AGT GKGS C++ S+++ ++TG FTSPHL VR+ ++ V IS F
Sbjct: 84 LKMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFL 143
Query: 82 TI------KAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWCILEVGLGGRLDATNV 135
+ + L++ Y A L Y + ILEVGLGG+ DATN
Sbjct: 144 GYFWWCYNRLKFWYLEKLYYWNYFHRGGGDAVELMY-SVFPVDAAILEVGLGGKFDATNA 202
Query: 136 IPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRVVEDK 195
+ K VCGI+ +G DH LG+ L IA EKAGI G V D +RV+E+K
Sbjct: 203 VQ-KPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVPAFTV--PQPDEAMRVLEEK 259
Query: 196 ARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQILD-YMQRTGM 254
A + P T+ + +L L+GE+Q N +A+ + ++Q+ G
Sbjct: 260 ASETEVNLEVVQPLTA---------RLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGK 310
Query: 255 IQLDS--------NQILDGIQTAEWPGRLQFINYHYSPRKTTPVL---FDGAHNGSAAVE 303
+++ S + + G+ TA GR Q + Y+ +T+ L DGAH+ +
Sbjct: 311 LEVPSRTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTSGDLVFYLDGAHSPESMEA 370
Query: 304 LAKY 307
AK+
Sbjct: 371 CAKW 374
>CE29283 [H] KOG2525 Folylpolyglutamate synthase
Length = 521
Score = 129 bits (325), Expect = 6e-30
Identities = 121/439 (27%), Positives = 197/439 (44%), Gaps = 67/439 (15%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISS------ 77
L ++H++GT GKGS C+++ S+L+ +TG ++SPHL HVR+ I V+ P+S
Sbjct: 99 LNIIHVSGTKGKGSACAFVESILRSQGLRTGFYSSPHLVHVRERIQVDGQPVSEQMFAEE 158
Query: 78 --HDFTTIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWCILEVGLGGRLDATNV 135
H + IK + Y F+ +T A F ++ ILEVG+GG D TNV
Sbjct: 159 FFHVYDIIKREHSDNMPAY------FKFLTLLAFRIFVKLNVQVMILEVGIGGEYDCTNV 212
Query: 136 IPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRVVEDK 195
+ K KVCG+T + DH S LGN L IA KAGI E T + V + +
Sbjct: 213 VE-KPKVCGVTTLDYDHMSILGNKLSEIAWHKAGIFKESVPAFYSPTTTEAEEVLIA--R 269
Query: 196 ARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQIL-DYMQRTGM 254
A S + +T P ++ + S G + G +Q +NV +A+Q++ + ++ G
Sbjct: 270 AISKHVPLFQTPPVSAYQFARDISPG--------IRGAHQFSNVSMALQLVRAWAEKCGF 321
Query: 255 ------IQLDSN------QILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGAHNGSAAV 302
+ D++ + D I++ WPGR Q + S + L DGAH +
Sbjct: 322 PLPGVPLSTDTSGFNVPLWMCDAIESCRWPGRSQIV----STDRNVTYLLDGAHTPKSME 377
Query: 303 ELAKYLDTEFRFSKDEPLTFVIAI---------TNGKTLEPL-LSPIIRPQDKVICTEFG 352
+++ E K E + ++ T K L+PL +S I+ ++ +
Sbjct: 378 ACSEWAAEEIVNLKKENVKKILLFQCTADRCPSTLIKYLKPLGISQIVSCPTQLHSSIDK 437
Query: 353 SVEGMPWIKPMDAY-DLASQVGHFTKN--------VEVNRDLFDILPALAQ------SGP 397
S + D + A+Q K E +FD + + + +
Sbjct: 438 SADSANLNASRDEQAEKANQCVQAWKESLDQPESVTEDQMKVFDCISSAYKFIESQAASQ 497
Query: 398 ETKVVVCGSLYLVGQLLNV 416
E V+V GSL+LVG +LN+
Sbjct: 498 EILVLVTGSLHLVGGVLNL 516
>CE01923 [H] KOG2525 Folylpolyglutamate synthase
Length = 510
Score = 129 bits (325), Expect = 6e-30
Identities = 121/439 (27%), Positives = 197/439 (44%), Gaps = 67/439 (15%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISS------ 77
L ++H++GT GKGS C+++ S+L+ +TG ++SPHL HVR+ I V+ P+S
Sbjct: 88 LNIIHVSGTKGKGSACAFVESILRSQGLRTGFYSSPHLVHVRERIQVDGQPVSEQMFAEE 147
Query: 78 --HDFTTIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWCILEVGLGGRLDATNV 135
H + IK + Y F+ +T A F ++ ILEVG+GG D TNV
Sbjct: 148 FFHVYDIIKREHSDNMPAY------FKFLTLLAFRIFVKLNVQVMILEVGIGGEYDCTNV 201
Query: 136 IPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRVVEDK 195
+ K KVCG+T + DH S LGN L IA KAGI E T + V + +
Sbjct: 202 VE-KPKVCGVTTLDYDHMSILGNKLSEIAWHKAGIFKESVPAFYSPTTTEAEEVLIA--R 258
Query: 196 ARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQIL-DYMQRTGM 254
A S + +T P ++ + S G + G +Q +NV +A+Q++ + ++ G
Sbjct: 259 AISKHVPLFQTPPVSAYQFARDISPG--------IRGAHQFSNVSMALQLVRAWAEKCGF 310
Query: 255 ------IQLDSN------QILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGAHNGSAAV 302
+ D++ + D I++ WPGR Q + S + L DGAH +
Sbjct: 311 PLPGVPLSTDTSGFNVPLWMCDAIESCRWPGRSQIV----STDRNVTYLLDGAHTPKSME 366
Query: 303 ELAKYLDTEFRFSKDEPLTFVIAI---------TNGKTLEPL-LSPIIRPQDKVICTEFG 352
+++ E K E + ++ T K L+PL +S I+ ++ +
Sbjct: 367 ACSEWAAEEIVNLKKENVKKILLFQCTADRCPSTLIKYLKPLGISQIVSCPTQLHSSIDK 426
Query: 353 SVEGMPWIKPMDAY-DLASQVGHFTKN--------VEVNRDLFDILPALAQ------SGP 397
S + D + A+Q K E +FD + + + +
Sbjct: 427 SADSANLNASRDEQAEKANQCVQAWKESLDQPESVTEDQMKVFDCISSAYKFIESQAASQ 486
Query: 398 ETKVVVCGSLYLVGQLLNV 416
E V+V GSL+LVG +LN+
Sbjct: 487 EILVLVTGSLHLVGGVLNL 505
>HsM4826728 [H] KOG2525 Folylpolyglutamate synthase
Length = 545
Score = 129 bits (323), Expect = 1e-29
Identities = 95/306 (31%), Positives = 142/306 (46%), Gaps = 44/306 (14%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISSHDFTTI 83
L ++H+ GT GKGS C++ +L+ KTG F+SPHL VR+ I +N PIS FT
Sbjct: 54 LNIIHVTGTKGKGSTCAFTECILRSYGLKTGFFSSPHLVQVRERIRINGQPISPELFTKY 113
Query: 84 KAQL-EQLDERYSLKCTE----FELMTATALSYFNQVKCSWCILEVGLGGRLDATNVIPG 138
+L +L+E C F +T A F Q K ++EVG+GG D TN+I
Sbjct: 114 FWRLYHRLEETKDGSCVSMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNII-R 172
Query: 139 KNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRVVEDKARS 198
K VCG++ +G+DH S LG+ + IA +K GI +G V + + V+ D+A+
Sbjct: 173 KPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTV--LQPEGPLAVLRDRAQQ 230
Query: 199 VGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQI----LDYMQRTG- 253
+ + + + P+ L L GE+Q +N +A+Q+ L R G
Sbjct: 231 ISCPLYL----CPMLEALEEGGPPLTLG---LEGEHQRSNAALALQLAHCWLQRQDRHGA 283
Query: 254 ------------------MIQLDSNQILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGA 295
+ Q S+ L G++ EWPGR Q + R DGA
Sbjct: 284 GEPKASRPGLLWQLPLAPVFQPTSHMRL-GLRNTEWPGRTQVLR-----RGPLTWYLDGA 337
Query: 296 HNGSAA 301
H S+A
Sbjct: 338 HTASSA 343
>At5g41480 [H] KOG2525 Folylpolyglutamate synthase
Length = 480
Score = 127 bits (320), Expect = 2e-29
Identities = 124/443 (27%), Positives = 200/443 (44%), Gaps = 56/443 (12%)
Query: 4 DLGLLRLTQLLSHLGNPHLNLKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCH 63
DLG R+ +L+ L NPH KV+H+AGT GKGS ++L+++L+ Y G ++SPH+
Sbjct: 57 DLG--RMKRLMLRLRNPHYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILS 114
Query: 64 VRDSITVNQVPISSHD----FTTIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSW 119
+++ I+ N P+S+ F ++K LEQ + + + FE++T A S F +
Sbjct: 115 IKERISCNGEPVSASTLNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDI 174
Query: 120 CILEVGLGGRLDATNVIPGKNKVCG-ITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFV 178
++E GLGG DATNVI N IT IG + L S AA V + +
Sbjct: 175 AVIEAGLGGARDATNVIESSNLAASVITTIGGPFLPHIEGILRSKAAS-----VSSSVIL 229
Query: 179 AVDGTNDDAVVRVVEDK----ARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLN--G 232
A + + ++ ++ +S I D I ++LS++ L G
Sbjct: 230 ASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPI----------VELSDVNLRMLG 279
Query: 233 EYQVTNVQVAIQILDYMQRTGMIQLDSNQILDGIQTAEWPGRLQFINYHYSPRKTTP--- 289
+Q+ N A + ++ G ++ I G++ GR QF+ + P
Sbjct: 280 HHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGAT 339
Query: 290 VLFDGAHNGSAAVELAKYLDTEFRFSKDEPLTFVIAITNGKT----LEPLLSPIIRPQDK 345
VL DGAH +A L + + +F ++ L FV+A+ + K + LLS + +
Sbjct: 340 VLLDGAHTKESARALKEMIKKDF---PEKRLVFVVAMASDKDHVSFAKELLSGL--KPEA 394
Query: 346 VICTEF-----------GSVEGMPWIKPMDAYDLASQVGHFTKNVEVNRDL---FDILPA 391
VI TE SV WIK D +L S+ ++N V L + IL
Sbjct: 395 VILTEADIGGGKIRSTESSVLKESWIKAAD--ELGSRSMEASENKTVLGSLKLAYKILSD 452
Query: 392 LAQSGPETKVVVCGSLYLVGQLL 414
S V+V GSL++V +L
Sbjct: 453 DTTSSDSGMVIVTGSLHIVSSVL 475
>At3g55630 [H] KOG2525 Folylpolyglutamate synthase
Length = 498
Score = 127 bits (320), Expect = 2e-29
Identities = 98/310 (31%), Positives = 146/310 (46%), Gaps = 38/310 (12%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISSHDFTT- 82
+K++H+AGT GKGS C++ S+L+ +TG FTSPHL VR+ +N + IS F
Sbjct: 58 MKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNY 117
Query: 83 IKAQLEQLDERYSLKC---TEFELMTATALSYFNQVKCSWCILEVGLGGRLDATNVIPGK 139
+L E+ S + T F + A F + ILEVGLGGR DATNV P
Sbjct: 118 FWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVKP-- 175
Query: 140 NKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRVVEDKAR-- 197
VCGI+ +G DH LG L IAAEKAGI G V D +RV+ +KA
Sbjct: 176 -VVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTV--AQPDEAMRVLNEKASKL 232
Query: 198 SVGAEIVKTNPDTSIKSVVTSSWGPIDLSE-LPLNGEYQVTNVQVAIQI-------LDYM 249
V ++V+ P+D S+ L L GE+Q N +A+ + +
Sbjct: 233 EVNLQVVE----------------PLDSSQRLGLQGEHQYLNAGLAVALCSTFLKEIGIE 276
Query: 250 QRTGMIQLDS--NQILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGAHNGSAAVELAKY 307
+ G+ Q + + + G+ A GR + P + L DGAH+ + A +
Sbjct: 277 DKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSELPEEIVYYL-DGAHSPESMEACAIW 335
Query: 308 LDTEFRFSKD 317
+ + +++
Sbjct: 336 FSKQIKQNQE 345
>YOR241w [H] KOG2525 Folylpolyglutamate synthase
Length = 548
Score = 121 bits (303), Expect = 2e-27
Identities = 116/447 (25%), Positives = 185/447 (40%), Gaps = 79/447 (17%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQ---STYKTGKFTSPHLCHVRDSITVNQVPISSHDF 80
L ++HI GT GKGS ++ +S+L Q + G +TSPHL VR+ I +N PIS F
Sbjct: 120 LNIVHITGTKGKGSTAAFTSSILGQYKEQLPRIGLYTSPHLKSVRERIRINGEPISEEKF 179
Query: 81 TTIKAQL-EQLDERYSL-----------KCTEFELMTATALSYFNQVKCSWCILEVGLGG 128
++ ++LD S K F+ +T + F Q C C+ EVG+GG
Sbjct: 180 AKYFFEVWDRLDSTTSSLDKFPHMIPGSKPGYFKFLTLLSFHTFIQEDCKSCVYEVGVGG 239
Query: 129 RLDATNVIPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAV 188
LD+TN+I K VCG+T +G+DH LG+ + IA K GI G V+ +
Sbjct: 240 ELDSTNIIE-KPIVCGVTLLGIDHTFMLGDTIEEIAWNKGGIFKSGAPAFTVEKQPPQGL 298
Query: 189 VRVVEDKARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQILDY 248
+++++A + + P +++V +L + GE+Q +N +A+ +
Sbjct: 299 T-ILKERAEERKTTLTEVPPFKQLENV-----------KLGIAGEFQKSNASLAVMLASE 346
Query: 249 MQRTG-----MIQLDSN-----QILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGAHNG 298
+ T I+ SN + + G+Q +W GR Q + + DGAH
Sbjct: 347 ILHTSNILEEKIKCSSNASIPEKFIIGLQNTKWEGRCQVLE-----KGKNVWYIDGAHTK 401
Query: 299 SAAVELAKYLDTEFRFSKDEPLTF-------VIAITNGKTLEPLLSPIIRPQDKVICTEF 351
+ V + + R SK + + A+ N L +SP I D + T
Sbjct: 402 DSMVAASTWFRDMVRLSKRKKILLFNQQSRDANALVN--NLYSSVSPEITFDDVIFTTNV 459
Query: 352 GSVEGM----------------------PWIKPMDAYDLASQVGHFTKNVEVNRDLFDIL 389
G +K + D H T ++E +L + L
Sbjct: 460 TWKSGSYSADLVSMNTSQEDVEKLKVQESLVKNWNKIDDNRAKTHVTASIEEANELIETL 519
Query: 390 PALAQSGPETKVVVCGSLYLVGQLLNV 416
+ V GSL+LVG LL V
Sbjct: 520 -----YDEPADIFVTGSLHLVGGLLVV 541
>SPBC1709.17 [H] KOG2525 Folylpolyglutamate synthase
Length = 505
Score = 120 bits (302), Expect = 3e-27
Identities = 102/337 (30%), Positives = 158/337 (46%), Gaps = 50/337 (14%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYK--------TGKFTSPHLCHVRDSITVNQVPI 75
L V+H+AGT GKGS C++ +S+LQQ G +TSPHL V + I +N PI
Sbjct: 79 LNVIHVAGTKGKGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKPI 138
Query: 76 SSHDFT----TIKAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWCILEVGLGGRLD 131
S FT + +LE S K F +T A F I+EVG+GG D
Sbjct: 139 SQELFTKYFFDVWERLENAVGSDSEKPMYFRFLTLMAWHVFISENVDAAIIEVGIGGEYD 198
Query: 132 ATNVIPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRV 191
+TN+I K +T +GLDH S LGN + IA +KAGI E +A+ + V
Sbjct: 199 STNLIE-KPYATAVTSLGLDHTSLLGNTIAEIAWQKAGIYKESA--IALTCEQAPEAMNV 255
Query: 192 VEDKARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQIL-DYMQ 250
++++A +TS+K V+ + D+ + L+G +Q+ N +A+ ++ ++ +
Sbjct: 256 LKNRA---------AERNTSLKVVIPPAELTPDM--IGLSGVHQLGNTSLAVSLVQEFYE 304
Query: 251 RTGM-IQLDSNQ---ILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGAHNGSA------ 300
+ G D Q ILDG++ +WPGR Q + FDGAH +
Sbjct: 305 KAGCPFDRDPYQDPAILDGLKYVKWPGRCQIEEIN-----NIKWCFDGAHTKESLEATGL 359
Query: 301 --AVELAKYLDTEFRF------SKDEPLTFVIAITNG 329
A + Y D + R S+D+P+ + + G
Sbjct: 360 WLASKKNLYEDADARILLFNQQSRDDPIALLRSFLKG 396
>7292786 [H] KOG2525 Folylpolyglutamate synthase
Length = 522
Score = 112 bits (281), Expect = 8e-25
Identities = 90/329 (27%), Positives = 139/329 (41%), Gaps = 40/329 (12%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISSHDFTTI 83
L +H+AGT GKGS C+ S+L+ ++TG F+SPH+ + I ++ +S FT
Sbjct: 115 LSFIHVAGTKGKGSTCALTESLLRHQGFRTGFFSSPHILFTNERIRIDGQLLSKDKFT-- 172
Query: 84 KAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWCILEVGLGGRLDATNVIPGKNKVC 143
EQ ++ +T F +LEVG+GG D TN++ V
Sbjct: 173 ----EQF----------WKFLTILGFHVFVAENVDVVVLEVGIGGEHDCTNIVRNVRTV- 217
Query: 144 GITKIGLDHESFLGNDLPSIAAEKAGILVEGTRFVAVDGTNDDAVVRVVEDKARSVGAEI 203
GIT +GL+H LG LP IA +KAGI+ G+ + V+ + + A +
Sbjct: 218 GITSLGLEHTELLGRTLPEIAWQKAGIIKTGSH--VFTHVTQPECLEVIRQRTKEHSATL 275
Query: 204 VKTNP--DTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQILDYMQRTGMIQLDSN- 260
+ P D W + + LNG + Q D++ ++G Q N
Sbjct: 276 YEVPPTEDYFRSKAYAPIWQTFS-NLIRLNGSLAIQLAQ------DWLSQSGKQQHTPNE 328
Query: 261 -----QILDGIQTAEWPGRLQFINYHYSPRKTTPVLFDGAHNGSAAVELAKYLDTEFRFS 315
Q+LDG+ + WPGR Q I +H + DGAH + + + R S
Sbjct: 329 VKMDPQLLDGLISTHWPGRCQLIEWH-----GMRLHLDGAHTLESMEVCTDWFEKNVRDS 383
Query: 316 KDEPLTFVIAITNGKTLEPLLSPIIRPQD 344
+ P + T PLL + R D
Sbjct: 384 VN-PKILIFNRTGESGFAPLLKLLNRTCD 411
>7295749_2 [H] KOG2525 Folylpolyglutamate synthase
Length = 598
Score = 87.0 bits (214), Expect = 5e-17
Identities = 77/271 (28%), Positives = 122/271 (44%), Gaps = 26/271 (9%)
Query: 24 LKVLHIAGTNGKGSVCSYLNSVLQQSTYKTGKFTSPHLCHVRDSITVNQVPISSHDFTTI 83
+ V+ +AG+ G+GS C+ + S+L+ KTG +SPHL + I ++ P+S FT +
Sbjct: 51 IPVIQVAGSKGRGSTCAIVESILRCHGVKTGVLSSPHLFLTSERIRIDGEPLSDVQFTEL 110
Query: 84 KAQLEQLDERYSLKCTEFELMTATALSYFNQVKCSWCILEVGLGGRLDATNVIPGKNKVC 143
++ + ++MT A F+Q ILEVG G DATN I +
Sbjct: 111 FWKINTDLANMQPTPSYNKIMTVMAFHAFHQAGVEVAILEVGNAGASDATN-IASHAQTI 169
Query: 144 GITKIGLDHESFLGNDLPSIAAEKAGIL-VEGTRFVAVDGTNDDAVVRVVEDKARSVGAE 202
GIT +G + S LGN L IA KA I+ E + V T V+ KA+ +GA+
Sbjct: 170 GITTLGWEQSSNLGNSLRDIAWAKASIMKPEANIYTNVTQTE---CCEVLAQKAKQIGAQ 226
Query: 203 IVK-------TNPDTSIKSVVTSSWGPIDLSELPLNGEYQVTNVQVAIQILDYMQRTGMI 255
+ + D + K ++ + + LNG VQ+A L + ++
Sbjct: 227 LRRVPTFNDYVEGDMNNKLLMNKA-----NYSMRLNGSLA---VQLAYDFLKRHKPEYVV 278
Query: 256 QLDSNQIL------DGIQTAEWPGRLQFINY 280
L+ N L GI+ E PG F+ +
Sbjct: 279 GLEHNSTLLTPGASRGIEIFEQPGHFDFMRH 309
>At3g10160 [H] KOG2525 Folylpolyglutamate synthase
Length = 442
Score = 83.6 bits (205), Expect = 5e-16
Identities = 86/324 (26%), Positives = 139/324 (42%), Gaps = 44/324 (13%)
Query: 56 FTSPHLCHVRDSITVNQVPISSHDFTTIKAQLEQLDERYSLKCTE----FELMTATALSY 111
FTSPHL VR+ ++ + IS F + +L + ++ F+ +T A
Sbjct: 2 FTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKEKAVDGLTMPPLFQFLTVLAFKI 61
Query: 112 FNQVKCSWCILEVGLGGRLDATNVIPGKNKVCGITKIGLDHESFLGNDLPSIAAEKAGIL 171
F K ++EVGLGG+LD+TNVI K VCGI +G+DH LGN L IA KAGI
Sbjct: 62 FVCEKVDVAVIEVGLGGKLDSTNVIQ-KPVVCGIASLGMDHMDILGNTLADIAFHKAGIF 120
Query: 172 VEGTRFVAVDGTNDDAVVRVVEDKARSVGAEIVKTNPDTSIKSVVTSSWGPIDLSELPLN 231
V ++ + V++ A ++ IV + V L L+
Sbjct: 121 KPQIPAFTVPQLSE--AMDVLQKTANNLEVTIVAPLEPKKLDGVT-----------LGLS 167
Query: 232 GEYQVTNVQVAIQILD-YMQRTG----MIQLDSNQ------ILDGIQTAEWPGRLQFIN- 279
G++Q+ N +A+ + ++QRTG + +S + G+ TA GR Q ++
Sbjct: 168 GDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARLHGRAQVVHD 227
Query: 280 -----YHYSPRKTTP-----VLFDGAHNGSAAVELAKYLDTEFRFSKDEPLTFVIAITNG 329
S TP DGAH+ + ++ + R D+ L+ I + N
Sbjct: 228 VVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVR--GDKSLSTAILLFN- 284
Query: 330 KTLEPLLSPIIRPQDKVICTEFGS 353
+E ++ P+ C G+
Sbjct: 285 -CMEVRDPQVLLPKLVTTCASSGT 307
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,156,619
Number of Sequences: 60738
Number of extensions: 1077971
Number of successful extensions: 2351
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2290
Number of HSP's gapped (non-prelim): 20
length of query: 422
length of database: 30,389,216
effective HSP length: 109
effective length of query: 313
effective length of database: 23,768,774
effective search space: 7439626262
effective search space used: 7439626262
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)