ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3203 check: MH EH KOG4166 Amino acid transport and metabolism Thiamine pyrophosphate-requiring enzyme
r_klactII3203 check: MH EH KOG4166 Coenzyme transport and metabolism Thiamine pyrophosphate-requiring enzyme
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3203 1102564 1100492 -691
(691 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR108w [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 988 0.0
SPBP35G2.07 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 713 0.0
At3g48560 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 449 e-126
7302414 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 140 5e-33
At5g17380 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 137 4e-32
Hs21361361 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 134 4e-31
CE19658 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 132 1e-30
CE02935 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 132 1e-30
HsM5803042 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 132 2e-30
Hs6912418 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 123 8e-28
SPBC725.04 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 121 4e-27
YEL020c [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 112 2e-24
CE26009 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 102 2e-21
SPAC186.09 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 94 5e-19
YLR134w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 88 5e-17
YLR044c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 88 5e-17
SPAC1F8.07c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 87 7e-17
YGR087c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 81 5e-15
At5g01320 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 71 6e-12
YDL080c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 69 2e-11
At5g01330 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 64 6e-10
At4g33070 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 64 8e-10
At5g54960 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 61 7e-09
>YMR108w [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 687
Score = 988 bits (2554), Expect = 0.0
Identities = 489/648 (75%), Positives = 551/648 (84%), Gaps = 14/648 (2%)
Query: 44 RPQPAPSFNADIAGGSSGGARRNQIPLSKRSRIADKNTGVNIQNQMDDSFIGMTGGEIFH 103
RP+PAPSFN D PL + + + + + MD SF+G+TGG+IF+
Sbjct: 54 RPEPAPSFNVD--------------PLEQPAEPSKLAKKLRAEPDMDTSFVGLTGGQIFN 99
Query: 104 EMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGKPGVV 163
EMM R NVDTVFGYPGGAILPVYDAI+NSDKF FVLPKHEQGAGHMAEGYARASGKPGVV
Sbjct: 100 EMMSRQNVDTVFGYPGGAILPVYDAIHNSDKFNFVLPKHEQGAGHMAEGYARASGKPGVV 159
Query: 164 LVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSCTKWNVM 223
LVTSGPGATNVVTPMADA ADG+P+VVFTGQV TSAIGTDAFQEADVVGISRSCTKWNVM
Sbjct: 160 LVTSGPGATNVVTPMADAFADGIPMVVFTGQVPTSAIGTDAFQEADVVGISRSCTKWNVM 219
Query: 224 VKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTLNQLTQR 283
VK++EELP RINEAFEIATSGRPGPVLVDLPKDVTAA+LRNPIP K+ LPS+ LNQLT R
Sbjct: 220 VKSVEELPLRINEAFEIATSGRPGPVLVDLPKDVTAAILRNPIPTKTTLPSNALNQLTSR 279
Query: 284 VVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLG 343
DEF +++I++AADLIN AKKP+LYVGAGIL GP+ LK+LSDRAQIPVTTTLQGLG
Sbjct: 280 AQDEFVMQSINKAADLINLAKKPVLYVGAGILNHADGPRLLKELSDRAQIPVTTTLQGLG 339
Query: 344 AFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVTLNISKFAPEARKAALEK 403
+F+QED KSLDM+GMHG A ANLA+QNADLIIA+GARFDDRVT NISKFAPEAR+AA E
Sbjct: 340 SFDQEDPKSLDMLGMHGCATANLAVQNADLIIAVGARFDDRVTGNISKFAPEARRAAAEG 399
Query: 404 RGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYP 463
RGGI+HFE+SPKNINK GD T NL K++ + V ER EW AQ+ KWK+EYP
Sbjct: 400 RGGIIHFEVSPKNINKVVQTQIAVEGDATTNLGKMMSKIFPVKERSEWFAQINKWKKEYP 459
Query: 464 YAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITS 523
YAY +ETPGSKIKPQTVI KLS + +GR IVTTGVGQHQMWAAQHWTW++P +FITS
Sbjct: 460 YAYMEETPGSKIKPQTVIKKLSKVANDTGRHVIVTTGVGQHQMWAAQHWTWRNPHTFITS 519
Query: 524 GGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNNEE 583
GGLGTMGYGLPAAIGAQVA+P+++VIDIDGDASFNMTL ELSSAVQA TPVK+L+LNNEE
Sbjct: 520 GGLGTMGYGLPAAIGAQVAKPESLVIDIDGDASFNMTLTELSSAVQAGTPVKILILNNEE 579
Query: 584 QGMVTQWQSLFYEHRYSHTHQLNPDFVKLAEAMGLKALRLSKQEDTDAILKEFVDYEGPI 643
QGMVTQWQSLFYEHRYSHTHQLNPDF+KLAEAMGLK LR+ KQE+ DA LKEFV +GP+
Sbjct: 580 QGMVTQWQSLFYEHRYSHTHQLNPDFIKLAEAMGLKGLRVKKQEELDAKLKEFVSTKGPV 639
Query: 644 LLEVQIEKKVPVLPMVPTGKGLHEFINFDPEVEKEQNELRHKRTSGKY 691
LLEV+++KKVPVLPMV G GL EFINFDPEVE++Q ELRHKRT GK+
Sbjct: 640 LLEVEVDKKVPVLPMVAGGSGLDEFINFDPEVERQQTELRHKRTGGKH 687
>SPBP35G2.07 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 669
Score = 713 bits (1841), Expect = 0.0
Identities = 356/585 (60%), Positives = 444/585 (75%), Gaps = 7/585 (1%)
Query: 90 DDSFIGMTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHM 149
D SF+G TGGEIFH+MM +HNV VFGYPGGAILPV+DAIY S F F+LP+HEQ AGH
Sbjct: 81 DSSFVGKTGGEIFHDMMLKHNVKHVFGYPGGAILPVFDAIYRSPHFEFILPRHEQAAGHA 140
Query: 150 AEGYARASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEAD 209
A+ Y+R + KPGVVLVTSGPGATNV+TP+ADALADG PLVVF+GQVATSAIG+DAFQEAD
Sbjct: 141 AQAYSRVTKKPGVVLVTSGPGATNVITPIADALADGTPLVVFSGQVATSAIGSDAFQEAD 200
Query: 210 VVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMK 269
+VGISRSCTKWNVMVK + +LPRRI+EAFEIATSGRPGPVLVDLPKDVTA+VL+ PIP+
Sbjct: 201 MVGISRSCTKWNVMVKDVADLPRRIDEAFEIATSGRPGPVLVDLPKDVTASVLKEPIPIL 260
Query: 270 SILPSDTLNQLTQRVVDEFT---LENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQ 326
S +PS +N+ + V++E + I R A+L+ AKKP+++ G G+L + P L++
Sbjct: 261 SSVPS--MNRRMKEVLEEGSKNVTAKIDRVANLLKLAKKPVIFCGHGVLANPECPTLLRK 318
Query: 327 LSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVT 386
S+R QIPVTT+L GLGA ++ SL M+GMHG+ AN+A+Q ADLI+ALG RFDDRVT
Sbjct: 319 FSERLQIPVTTSLLGLGAVDERSDLSLHMLGMHGSGYANMAMQEADLILALGVRFDDRVT 378
Query: 387 LNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVES-- 444
N+S FAP+AR AA E+RGGI+HF+ISPKNI K GDV +L+ L ++
Sbjct: 379 GNVSLFAPQARLAAAEERGGIIHFDISPKNIGKVVQPTEAIEGDVYESLKLLDSATKNIK 438
Query: 445 VPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVTTGVGQH 504
+P R +W +Q+ WK+ +P+ + + PG +KPQ VI +L +TTGVG H
Sbjct: 439 IPSRFDWLSQIQTWKERFPFTFTRSAPGELVKPQEVIQELDKQTSDIKDKVTITTGVGAH 498
Query: 505 QMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNMTLMEL 564
QMWAA + W P S +TSGGLGTMG+GLPAAIGA VA P IVIDIDGDASF+MT MEL
Sbjct: 499 QMWAATFYRWTKPSSLVTSGGLGTMGFGLPAAIGASVAAPKDIVIDIDGDASFSMTGMEL 558
Query: 565 SSAVQARTPVKVLLLNNEEQGMVTQWQSLFYEHRYSHTHQLNPDFVKLAEAMGLKALRLS 624
++ Q PVK+L+LNNEEQGMVTQWQ+LFYE RYSHTHQ NP+FVKLA+AMG+KALR+
Sbjct: 559 ATVRQFDIPVKILILNNEEQGMVTQWQNLFYEKRYSHTHQKNPNFVKLADAMGIKALRVE 618
Query: 625 KQEDTDAILKEFVDYEGPILLEVQIEKKVPVLPMVPTGKGLHEFI 669
K+ED +KEF+ +GP+L+EV + +K V P VP GK LH+FI
Sbjct: 619 KREDLAKKMKEFLSTKGPVLMEVLVAQKEHVYPFVPGGKALHQFI 663
>At3g48560 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 670
Score = 449 bits (1156), Expect = e-126
Identities = 271/639 (42%), Positives = 373/639 (57%), Gaps = 50/639 (7%)
Query: 59 SSGGARRNQIPLSKRSRI-ADKNTGVNIQNQMD-------DSFIGM-------TGGEIFH 103
SS +RR I S S I A NT N+ ++FI G +I
Sbjct: 45 SSSSSRRRGIKSSSPSSISAVLNTTTNVTTTPSPTKPTKPETFISRFAPDQPRKGADILV 104
Query: 104 EMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGKPGVV 163
E ++R V+TVF YPGGA + ++ A+ S VLP+HEQG AEGYAR+SGKPG+
Sbjct: 105 EALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPGIC 164
Query: 164 LVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSCTKWNVM 223
+ TSGPGATN+V+ +ADAL D VPLV TGQV IGTDAFQE +V ++RS TK N +
Sbjct: 165 IATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYL 224
Query: 224 VKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTLNQLTQR 283
V +E++PR I EAF +ATSGRPGPVLVD+PKD+ + LP ++++ +
Sbjct: 225 VMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPG-YMSRMPKP 283
Query: 284 VVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLG 343
D LE I R LI+ +KKP+LYVG G L S L + + IPV +TL GLG
Sbjct: 284 PEDSH-LEQIVR---LISESKKPVLYVGGGCLNSS---DELGRFVELTGIPVASTLMGLG 336
Query: 344 AFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVTLNISKFAPEARKAALEK 403
++ +D SL M+GMHG AN A++++DL++A G RFDDRVT + FA A+
Sbjct: 337 SYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAFASRAK------ 390
Query: 404 RGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESVPERPE-----WSAQVAKW 458
IVH +I I K GDV L+ + ++E+ E + W ++
Sbjct: 391 ---IVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNELNVQ 447
Query: 459 KQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVTTGVGQHQMWAAQHWTWKSPR 518
KQ++P ++ +T G I PQ I L +L + AI++TGVGQHQMWAAQ + +K PR
Sbjct: 448 KQKFPLSF--KTFGEAIPPQYAIKVLDEL---TDGKAIISTGVGQHQMWAAQFYNYKKPR 502
Query: 519 SFITSGGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNMTLMELSSAVQARTPVKVLL 578
+++SGGLG MG+GLPAAIGA VA PDAIV+DIDGD SF M + EL++ PVKVLL
Sbjct: 503 QWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVKVLL 562
Query: 579 LNNEEQGMVTQWQSLFYEHRYSHT--------HQLNPDFVKLAEAMGLKALRLSKQEDTD 630
LNN+ GMV QW+ FY+ +HT ++ P+ + A A G+ A R++K+ D
Sbjct: 563 LNNQHLGMVMQWEDRFYKANRAHTFLGDPAQEDEIFPNMLLFAAACGIPAARVTKKADLR 622
Query: 631 AILKEFVDYEGPILLEVQIEKKVPVLPMVPTGKGLHEFI 669
++ +D GP LL+V + VLPM+P+G ++ I
Sbjct: 623 EAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGTFNDVI 661
>7302414 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 568
Score = 140 bits (354), Expect = 5e-33
Identities = 130/504 (25%), Positives = 220/504 (42%), Gaps = 61/504 (12%)
Query: 100 EIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGK 159
+I E +++ V+ VFG G ++ + A + + ++ ++EQ A + A+ +GK
Sbjct: 8 QIIAESLKQQGVEYVFGIIGIPVIELSMA-FQAAGLKYIGMRNEQAACYAAQAIGYLTGK 66
Query: 160 PGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSCTK 219
PGV LV SGPG +V MA+A + PL+V G G FQE V +SR K
Sbjct: 67 PGVCLVVSGPGLLHVTGGMANAQVNCWPLIVIGGATNQDHEGIGGFQECPQVELSRPYCK 126
Query: 220 WNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAA---------VLRNPIPMKS 270
+ T +P + +A AT GRPG +D P ++ + L +P P +
Sbjct: 127 YAARPATAALIPLHVEKAVRYATYGRPGVAYLDFPGNILQSKAQEASIYKALAHPAPPLA 186
Query: 271 ILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDR 330
P D ++ RAA L+ +AK+PL+ VG G + L+ +
Sbjct: 187 YPPHD----------------DVIRAAKLLRQAKRPLVIVGKGSAYAH-AENTLRHFIEN 229
Query: 331 AQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVTLNIS 390
+P T G G + D ++A LA+Q AD+++ LGAR + L+
Sbjct: 230 TNLPFLPTPMGKGVVS-------DTAPQCVSSARTLALQKADVVLLLGARLN--WILHFG 280
Query: 391 KFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESVP---- 446
K AP +K + +I+P+ ++ D+ E+L + +V
Sbjct: 281 K-APR-----YDKDVKFIQVDINPEELHNSVVASVAIQADIRPFAEQLFEQMNAVNFRFG 334
Query: 447 -ERPEWSAQVAKWKQE----YPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVTTGV 501
E+ W K KQ + ETP + V L +L+ K IV+ G
Sbjct: 335 YEQDWWKQLAVKCKQNRDTVQKMSLNTETP---LNYYAVFHHLRELLPKD--TIIVSEGA 389
Query: 502 GQHQMWAAQHWTWKSPRSFITSGGLGTMGYG----LPAAIGAQVARPDAIVIDIDGDASF 557
+ + + PR + +G GTMG G + AA+ + P V+ ++GD++F
Sbjct: 390 NTMDIGRSMLLN-EQPRHRLDAGTFGTMGVGPGFAVAAALFCRDFAPGKRVLCVEGDSAF 448
Query: 558 NMTLMELSSAVQARTPVKVLLLNN 581
+ ME+ + V+ + PV ++++NN
Sbjct: 449 GFSGMEIETMVRYKLPVTIVIVNN 472
>At5g17380 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 572
Score = 137 bits (346), Expect = 4e-32
Identities = 141/546 (25%), Positives = 235/546 (42%), Gaps = 71/546 (13%)
Query: 137 FVLPKHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVA 196
F+ +EQ AG+ A Y +GKPG++L SGPG + + +++A + P+V+ +G
Sbjct: 52 FIAFHNEQSAGYAASAYGYLTGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCD 111
Query: 197 TSAIGTDAFQEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKD 256
+G FQE D + ++ +K + K + E+P ++ + A SGRPG +D+P D
Sbjct: 112 QRDVGRGDFQELDQIEAVKAFSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTD 171
Query: 257 VTAAVLRNPIPMKSILPSDTLNQLTQRVVDEFTL-----ENISRAADLINRAKKPLLYVG 311
VLR I S +D L +R E + I A L+ +A++PL+ G
Sbjct: 172 ----VLRQKI---SESEADKLVDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFG 224
Query: 312 AGILGSEMGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNA 371
G S + LK+L + IP T G G D AA +LAI
Sbjct: 225 KGAAYSRAEDE-LKKLVEITGIPFLPTPMGKGLLP-------DTHEFSATAARSLAIGKC 276
Query: 372 DLIIALGARFD-----------DR----VTLNISKFAPEARKAALEKRG------GIVHF 410
D+ + +GAR + D+ + +++S+ E RK L G G+++
Sbjct: 277 DVALVVGARLNWLLHFGESPKWDKDVKFILVDVSEEEIELRKPHLGIVGDAKTVIGLLNR 336
Query: 411 EISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYPYAYQKET 470
EI D L K VES+ ++ + + + K E A +
Sbjct: 337 EIK----------------DDPFCLGKSNSWVESISKKAKENGE----KMEIQLA-KDVV 375
Query: 471 PGSKIKPQTVISKLSDLVLKSGRPAIVTTGVGQHQMWAAQH-WTWKSPRSFITSGGLGTM 529
P + + P +I D +L P+ V G + M + K PR+ + +G GTM
Sbjct: 376 PFNFLTPMRII---RDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGTM 432
Query: 530 GYGLPAAIGAQVARPDAIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNN-----EEQ 584
G GL I A VA PD +V+ ++GD+ F + ME+ + V+ V +++ NN ++
Sbjct: 433 GVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGDR 492
Query: 585 GMVTQWQSLFYEHRYSHTHQLNPDFVKLAEAMGLKALRLSKQEDTDAILKEFVDYEGPIL 644
+ E + N + KL EA G K + ++ + L E P +
Sbjct: 493 RGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPAV 552
Query: 645 LEVQIE 650
+ V I+
Sbjct: 553 VNVIID 558
>Hs21361361 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 632
Score = 134 bits (338), Expect = 4e-31
Identities = 137/573 (23%), Positives = 240/573 (40%), Gaps = 55/573 (9%)
Query: 98 GGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARAS 157
GGE +++ H V +F GG I P+ A V +HE A A+ AR S
Sbjct: 54 GGENVAAVLRAHGVRFIFTLVGGHISPLLVACEKLG-IRVVDTRHEVTAVFAADAMARLS 112
Query: 158 GKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSC 217
G GV VT+GPG TN VT + +A P+++ G +T A Q D + + R
Sbjct: 113 GTVGVAAVTAGPGLTNTVTAVKNAQMAQSPILLLGGAASTLLQNRGALQAVDQLSLFRPL 172
Query: 218 TKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTL 277
K+ V V+ + ++ + A A SG PGPV V+LP DV + K ++P+
Sbjct: 173 CKFCVSVRRVRDIVPTLRAAMAAAQSGTPGPVFVELPVDVLYPYF---MVQKEMVPAKPP 229
Query: 278 NQLTQRVVDEFTLEN-----------------------------ISRAADLINRAKKPLL 308
L RVV + LEN + R ++++RAK+PL+
Sbjct: 230 KGLVGRVVSWY-LENYLANLFAGAWEPQPEGPLPLDIPQASPQQVQRCVEILSRAKRPLM 288
Query: 309 YVGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAI 368
+G+ L + L+ + +P G + +H + A+
Sbjct: 289 VLGSQALLTPTSADKLRAAVETLGVPCFLGGMARGLLGRNH-------PLHIRENRSAAL 341
Query: 369 QNADLIIALGARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXX 428
+ AD+I+ G D R L+ + + K + R + E N +
Sbjct: 342 KKADVIVLAGTVCDFR--LSYGRVLSHSSKIIIVNR----NREEMLLNSDIFWKPQEAVQ 395
Query: 429 GDVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYPYAYQKETP---GSKIKPQTVISKLS 485
GDV + KL+ ++ P+W ++ + ++ ++++ + P V+ +
Sbjct: 396 GDVGSFVLKLVEGLQGQTWAPDWVEELREADRQKEQTFREKAAMPVAQHLNPVQVLQLVE 455
Query: 486 DLVLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPD 545
+ + + +I+ G AA + P ++ G GT+G G A+GA++ RPD
Sbjct: 456 ETLPDN---SILVVDGGDFVGTAAHLVQPRGPLRWLDPGAFGTLGVGAGFALGAKLCRPD 512
Query: 546 AIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNNEEQGMVTQWQSLFYEHRYSHTHQL 605
A V + GD +F +L+E + V+ + PV L+ N+ +++ Q +
Sbjct: 513 AEVWCLFGDGAFGYSLIEFDTFVRHKIPVMALVGNDAGWTQISREQVPSLGSNVA-CGLA 571
Query: 606 NPDFVKLAEAMGLKALRLSKQEDTDAILKEFVD 638
D+ K A +G + L LS+ E+ D ++K D
Sbjct: 572 YTDYHKAAMGLGARGLLLSR-ENEDQVVKVLHD 603
>CE19658 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 634
Score = 132 bits (333), Expect = 1e-30
Identities = 128/518 (24%), Positives = 222/518 (42%), Gaps = 47/518 (9%)
Query: 79 KNTGVNIQNQMDDSFIGMTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFV 138
K++ +N + + M G I ++ V+ +FG G ++ V A + ++
Sbjct: 55 KDSRINSRKLRNHDGSNMDGASITAAALKSQGVEYMFGVVGFPVIEVGMAA-QAHGIKYI 113
Query: 139 LPKHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATS 198
++EQ A + A+ +GKP +LV SGPG + + +A+A + P+V G
Sbjct: 114 GCRNEQAAAYAAQAMGYLTGKPVALLVVSGPGILHAIGGLANATVNCWPVVCIGGTADVD 173
Query: 199 AIGTDAFQEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVT 258
AFQE R+ K ++ +P I +A A GRPG V VDLP ++
Sbjct: 174 LENRGAFQEWSQQESVRNSCKHVSRPTSLHTIPAHIEKAVRCAMYGRPGAVYVDLPGNLV 233
Query: 259 AAVLRNPIPMKSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSE 318
I +P V + I +A D + AKKPL+ VG G SE
Sbjct: 234 LTSTEEEISFPPQVP-------LPAPVSIPPIAEIEKAIDTLKSAKKPLVIVGKGAAWSE 286
Query: 319 MGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALG 378
G ++Q ++++P T G G + D+ A +LA++ AD + +G
Sbjct: 287 RGATQVQQFLTKSKLPWLATPGGKGV-------ASDLHPRFIGQARSLALREADTVFLIG 339
Query: 379 ARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKL 438
ARF+ I F R +K +V +I P+ ++ GD+ L +L
Sbjct: 340 ARFN-----WILHFGLPPR---FQKDVKVVQIDICPEEFHQNVKTEVPLLGDIGETLAEL 391
Query: 439 LPLVE--SVPERPEWSAQVAKWKQEYPYAYQK-----ETP---GSKIKP-QTVISKLSDL 487
P + + E EW ++ + ++ A +K TP + +P + ++ +
Sbjct: 392 TPRLGDWTYDESTEWFKKLRENAEKNRAAVEKFVDDHSTPLNYYAAYQPIREFLANNDVI 451
Query: 488 VLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYG----LPAAIGAQVAR 543
V+ G T +G+ M + + P+ + +G GTMG G L AA+ A+
Sbjct: 452 VINEGAN---TMDIGRTMMPS------RLPKRRLDAGTFGTMGVGHGFSLAAALWARDHS 502
Query: 544 PDAIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNN 581
P V+ + GD++F + MEL + + PV +++NN
Sbjct: 503 PKTKVLVVQGDSAFGFSAMELETIARYNLPVVTVIINN 540
>CE02935 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 634
Score = 132 bits (333), Expect = 1e-30
Identities = 128/518 (24%), Positives = 222/518 (42%), Gaps = 47/518 (9%)
Query: 79 KNTGVNIQNQMDDSFIGMTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFV 138
K++ +N + + M G I ++ V+ +FG G ++ V A + ++
Sbjct: 55 KDSRINSRKLRNHDGSNMDGASITAAALKSQGVEYMFGVVGFPVIEVGMAA-QAHGIKYI 113
Query: 139 LPKHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATS 198
++EQ A + A+ +GKP +LV SGPG + + +A+A + P+V G
Sbjct: 114 GCRNEQAAAYAAQAMGYLTGKPVALLVVSGPGILHAIGGLANATVNCWPVVCIGGTADVD 173
Query: 199 AIGTDAFQEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVT 258
AFQE R+ K ++ +P I +A A GRPG V VDLP ++
Sbjct: 174 LENRGAFQEWSQQESVRNSCKHVSRPTSLHTIPAHIEKAVRCAMYGRPGAVYVDLPGNLV 233
Query: 259 AAVLRNPIPMKSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSE 318
I +P V + I +A D + AKKPL+ VG G SE
Sbjct: 234 LTSTEEEISFPPQVP-------LPAPVSIPPIAEIEKAIDTLKSAKKPLVIVGKGAAWSE 286
Query: 319 MGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALG 378
G ++Q ++++P T G G + D+ A +LA++ AD + +G
Sbjct: 287 RGATQVQQFLTKSKLPWLATPGGKGV-------ASDLHPRFIGQARSLALREADTVFLIG 339
Query: 379 ARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKL 438
ARF+ I F R +K +V +I P+ ++ GD+ L +L
Sbjct: 340 ARFN-----WILHFGLPPR---FQKDVKVVQIDICPEEFHQNVKTEVPLLGDIGETLAEL 391
Query: 439 LPLVE--SVPERPEWSAQVAKWKQEYPYAYQK-----ETP---GSKIKP-QTVISKLSDL 487
P + + E EW ++ + ++ A +K TP + +P + ++ +
Sbjct: 392 TPRLGDWTYDESTEWFKKLRENAEKNRAAVEKFVDDHSTPLNYYAAYQPIREFLANNDVI 451
Query: 488 VLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYG----LPAAIGAQVAR 543
V+ G T +G+ M + + P+ + +G GTMG G L AA+ A+
Sbjct: 452 VINEGAN---TMDIGRTMMPS------RLPKRRLDAGTFGTMGVGHGFSLAAALWARDHS 502
Query: 544 PDAIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNN 581
P V+ + GD++F + MEL + + PV +++NN
Sbjct: 503 PKTKVLVVQGDSAFGFSAMELETIARYNLPVVTVIINN 540
>HsM5803042 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 632
Score = 132 bits (331), Expect = 2e-30
Identities = 136/573 (23%), Positives = 239/573 (40%), Gaps = 55/573 (9%)
Query: 98 GGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARAS 157
GGE +++ H V +F GG I P+ A V +HE A A+ AR S
Sbjct: 54 GGENVAAVLRAHGVRFIFTLVGGHISPLLVACEKLG-IRVVDTRHEVTAVFAADAMARLS 112
Query: 158 GKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSC 217
G GV VT+GPG TN VT + +A P+++ G +T A Q D + + R
Sbjct: 113 GTVGVAAVTAGPGLTNTVTAVKNAQMAQSPILLLGGAASTLLQNRGALQAVDQLSLFRPL 172
Query: 218 TKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTL 277
K+ V V+ + ++ + A A S PGPV V+LP DV + K ++P+
Sbjct: 173 CKFCVSVRRVRDIVPTLRAAMAAAQSXTPGPVFVELPVDVLYPYF---MVQKEMVPAKPP 229
Query: 278 NQLTQRVVDEFTLEN-----------------------------ISRAADLINRAKKPLL 308
L RVV + LEN + R ++++RAK+PL+
Sbjct: 230 KGLVGRVVSWY-LENYLANLFAGAWEPQPEGPLPLDIPQASPQQVQRCVEILSRAKRPLM 288
Query: 309 YVGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAI 368
+G+ L + L+ + +P G + +H + A+
Sbjct: 289 VLGSQALLTPTSADKLRAAVETLGVPCFLGGMARGLLGRNH-------PLHIRENRSAAL 341
Query: 369 QNADLIIALGARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXX 428
+ AD+I+ G D R L+ + + K + R + E N +
Sbjct: 342 KKADVIVLAGTVCDFR--LSYGRVLSHSSKIIIVNR----NREEMLLNSDIFWKPQEAVQ 395
Query: 429 GDVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYPYAYQKETP---GSKIKPQTVISKLS 485
GDV + KL+ ++ P+W ++ + ++ ++++ + P V+ +
Sbjct: 396 GDVGSFVLKLVEGLQGQTWAPDWVEELREADRQKEQTFREKAAMPVAQHLNPVQVLQLVE 455
Query: 486 DLVLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPD 545
+ + + +I+ G AA + P ++ G GT+G G A+GA++ RPD
Sbjct: 456 ETLPDN---SILVVDGGDFVGTAAHLVQPRGPLRWLDPGAFGTLGVGAGFALGAKLCRPD 512
Query: 546 AIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNNEEQGMVTQWQSLFYEHRYSHTHQL 605
A V + GD +F +L+E + V+ + PV L+ N+ +++ Q +
Sbjct: 513 AEVWCLFGDGAFGYSLIEFDTFVRHKIPVMALVGNDAGWTQISREQVPSLGSNVA-CGLA 571
Query: 606 NPDFVKLAEAMGLKALRLSKQEDTDAILKEFVD 638
D+ K A +G + L LS+ E+ D ++K D
Sbjct: 572 YTDYHKAAMGLGARGLLLSR-ENEDQVVKVLHD 603
>Hs6912418 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 578
Score = 123 bits (309), Expect = 8e-28
Identities = 140/578 (24%), Positives = 247/578 (42%), Gaps = 54/578 (9%)
Query: 96 MTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKF--TFVLPKHEQGAGHMAEGY 153
++G ++ + ++ +V+ +FG G +PV + + + ++ ++EQ A + A
Sbjct: 14 VSGAKVIAQALKTQDVEYIFGIVG---IPVTEIAIAAQQLGIKYIGMRNEQAACYAASAI 70
Query: 154 ARASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGI 213
+ +PGV LV SGPG + + MA+A + PL+V G + AFQE V
Sbjct: 71 GYLTSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQVEA 130
Query: 214 SRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKD-VTAAVLRNPIPMKSIL 272
R TK++ +IE +P I +A + GRPG VD+P D V V N I
Sbjct: 131 CRLYTKFSARXSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERC 190
Query: 273 PSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQ 332
S ++ V AA +I AK+PLL +G G + + +K+L ++ +
Sbjct: 191 MSPPISMAETSAV--------CTAASVIRNAKQPLLIIGKGAAYAH-AEESIKKLVEQYK 241
Query: 333 IPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVTLNI-SK 391
+P T G G ++H VG AA + A+Q AD+I+ GAR + + + +
Sbjct: 242 LPFLPTPMGKGVV-PDNHPY--CVG----AARSRALQFADVIVLFGARLNWILHFGLPPR 294
Query: 392 FAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESV-----P 446
+ P+ + + +I + + G++ ++LL ++ P
Sbjct: 295 YQPDVK---------FIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPP 345
Query: 447 ERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPA-IVTTGVGQHQ 505
E W K K A KE K P + + + R +V+ G
Sbjct: 346 ESKWWKTLREKMKSN--EAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMD 403
Query: 506 MWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPDAI----VIDIDGDASFNMTL 561
+ + PR + +G GTMG GL AI A V D +I ++GD++F +
Sbjct: 404 IGRTVLQNY-LPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGHWIICVEGDSAFGFSG 462
Query: 562 MELSSAVQARTPVKVLLLNNE--EQGMVTQ-WQSLF-YEHRYSHTHQL----NPDFVKLA 613
ME+ + + P+ +L++NN QG T W+ + ++ + + N + ++
Sbjct: 463 MEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVM 522
Query: 614 EAMGLKALRLSKQEDTDAILKE-FVDYEGPILLEVQIE 650
A G K + E+ L++ D P L+ + IE
Sbjct: 523 TAFGGKGYFVQTPEELQKSLEQSLADTTKPSLINIMIE 560
>SPBC725.04 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 568
Score = 121 bits (303), Expect = 4e-27
Identities = 127/563 (22%), Positives = 232/563 (40%), Gaps = 56/563 (9%)
Query: 111 VDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGKPGVVLVTSGPG 170
V VFG G ++ + +AI S FV ++EQ A + A Y + +PGV +V GPG
Sbjct: 18 VKVVFGIVGIPVIEICEAIQASG-IRFVGFRNEQSAAYAATAYGYLTQRPGVCVVVGGPG 76
Query: 171 ATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSCTKWNVMVKTIEEL 230
+ + + ++ + PL++ G T AFQE D V TK V + + +
Sbjct: 77 VVHAMAGVFNSKTNRWPLLLLAGSSETFQQNCGAFQELDQVSYLSPHTKLAVRPPSPKMV 136
Query: 231 PRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTLNQLTQRVVDEFTL 290
I A+ ++ +G PG VDLP + + + + P D L +
Sbjct: 137 VDSIRRAYRVSMTGTPGTCYVDLPANYIESTVDD-------FPKDPLPPIPSSPKCAPDP 189
Query: 291 ENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDH 350
+ +AA + AK PLL VG G + L + + IP + G G E H
Sbjct: 190 TQLQKAAYYLKNAKAPLLVVGKG-AAYACAEKQLLEFVEHTGIPFLPSPMGKGLL-PESH 247
Query: 351 KSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVTLNI-SKFAPEARKAALEKRGGIVH 409
++ ++A + A++NAD+++ GAR + + K++P A+ ++ +
Sbjct: 248 P------LNVSSARSAALRNADVVLLAGARLNWIFQYGLPPKWSPNAKFIQIDTNAETLG 301
Query: 410 FEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYPYAYQKE 469
N DV ++ L LV++ W V PY
Sbjct: 302 --------NNAADLDLAIWADVGLTIDCLFKLVQT------WKYSVG---ISTPYLRTLN 344
Query: 470 TPGSKIKPQTVISKLSDLVLKSGRPA-IVTTGVGQHQMWAAQHWTWKS------------ 516
SK + + + S+ S + L+ +V + + + ++ TW S
Sbjct: 345 ETRSKNEKKALESRKSSIPLQMNYALYVVNEELQSLSLKSKRNITWVSEGANTMDRGRQL 404
Query: 517 -----PRSFITSGGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNMTLMELSSAVQAR 571
PR + +G + TMG G+ AI + A ++ ++GD++F + MEL +A++ +
Sbjct: 405 LEVTHPRGRLDAGTMSTMGVGMGYAIASAFAHSSDKIVVVEGDSAFGFSAMELETAIRNQ 464
Query: 572 TPVKVLLLNNE--EQGMVTQWQSLFYEHRYSHTHQLNPD--FVKLAEAMGLKALRLSKQE 627
+ V+++NN G+ T ++ T L + ++ EA G K + +E
Sbjct: 465 LDLLVIVINNNGVYHGLDTDAYETLRDNHQLPTTALGTSIRYDQICEACGGKGFFVKNEE 524
Query: 628 DTDAILKEFVDYEGPILLEVQIE 650
D + L++ L+ V ++
Sbjct: 525 DLRSSLRKAWQTSSVSLINVMVD 547
>YEL020c [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 560
Score = 112 bits (279), Expect = 2e-24
Identities = 114/499 (22%), Positives = 222/499 (43%), Gaps = 43/499 (8%)
Query: 97 TGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARA 156
T + F +++Q++ +DTVFG G I+ + D + ++ F+ ++EQ A + A Y
Sbjct: 4 TATQHFAQLLQKYGIDTVFGIVGIPIVQLADTMV-ANGIKFIPCRNEQAASYAASAYGYI 62
Query: 157 SGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRS 216
S KPGV+L+ GPG + + + +++++ PL+V G + S I FQE D V +
Sbjct: 63 SDKPGVLLIVGGPGLIHALAGIYNSMSNRWPLLVIAGSSSQSDIHKGGFQELDQVSLLSP 122
Query: 217 CTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDT 276
K+ + T + + +A G G +D+P D P+ ++
Sbjct: 123 FLKFTGKL-TPDNIDMITQKALNYCIQGTAGVSYIDVPADFIE--YEKPLEGNDRTGNEL 179
Query: 277 LNQLTQRVVDEFTLENISRAADLI--NRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQIP 334
LT + I + LI ++ K L+ +G G + + +++L + +P
Sbjct: 180 PMILTPNICGP-DPSKIKKVVQLILQHKNKNILIVIGKGAVKNS---HEIRRLVNTFNLP 235
Query: 335 VTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQNADLIIALGARFDDRVTLNISKFAP 394
T G D ++ ++A + A++ AD+++ LGAR + + S P
Sbjct: 236 FLPTPMAKGIVP-------DSSPLNVSSARSQALKIADIVLVLGARLNWILHFGTS---P 285
Query: 395 EARKAALEKRGGIVHFEISPKNI---NKXXXXXXXXXGDVTRNLEKLLPLVESVPERPEW 451
+ ++ + F+ +P+ + N GD+ + + LVE + R +
Sbjct: 286 KWNSESI-----FIQFDSNPETLGDNNVSPGADLSIWGDIGLS---VTALVEEL-TRQDS 336
Query: 452 SAQVAKWKQEYPYAYQ---------KETPGSKIKPQTVISKLSDLVLKSGRPAIVTTGVG 502
+ + KQE Q ++T G+++ V L L+ R +VT G
Sbjct: 337 CWKYSGVKQEIREKIQLNQTRLLRKEKTRGAQLNYNQVYGTLRPLI-DDYRTILVTEGAN 395
Query: 503 QHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNMTLM 562
+ A + +PR + +G TMG GL A+ + + P+ V+ I GD++F + M
Sbjct: 396 TMDI-ARISFPTDAPRRRLDAGTNATMGIGLGYALACKASHPELDVVLIQGDSAFGFSAM 454
Query: 563 ELSSAVQARTPVKVLLLNN 581
E+ +AV+ + + ++++NN
Sbjct: 455 EIETAVRCQLALVIVVMNN 473
>CE26009 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 640
Score = 102 bits (255), Expect = 2e-21
Identities = 119/512 (23%), Positives = 193/512 (37%), Gaps = 56/512 (10%)
Query: 98 GGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARAS 157
GGE+ +++ H+V+ +F GG I P+ A V +HE A A+ AR
Sbjct: 58 GGELVASVLKAHDVEEIFVLCGGHISPILVAAEKLG-IKIVDTRHEVTAVFAADAVARLR 116
Query: 158 GKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADVVGISRSC 217
GV VT+GPG TN +T + +A PL++ G T G A Q+ D + + R
Sbjct: 117 QSIGVAAVTAGPGLTNTITAVKNAQMAESPLLLIGGAAPTLLKGRGALQDIDQMVLFRPL 176
Query: 218 TKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTL 277
K+ V+ + ++ + EA + A SG PGPV V+ P DV + + +K I +
Sbjct: 177 CKYVARVERLRDIVPTVREAIKAAKSGCPGPVFVEFPVDV---LYPYELVVKEIGFNPNA 233
Query: 278 NQLTQRVVDEF----------------------------TLENISRAADLINRAKKPLLY 309
QR ++ + E I L+ AK+P+L
Sbjct: 234 KGFIQRALNFYLRCHVSRQFGNAWAPQTITPLPTNIPMPKSEKIQEIVQLVKSAKRPVLL 293
Query: 310 VGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANLAIQ 369
+G+ + P L + + PV G ++ + V A++
Sbjct: 294 IGSQATLPPVKPADLVKAVEALGCPVFLGGMARGLLGKDHPLQMRQVRRD-------ALK 346
Query: 370 NADLIIALGARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXG 429
+ADL I G D R++ L K+ IV + + K
Sbjct: 347 DADLTILAGTVCDFRLSYG----------RTLSKKSKIVALNRNSSQLTKNEKAFWNSDV 396
Query: 430 DVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTV-------IS 482
V ++ L V + + K +KE+ +K Q + ++
Sbjct: 397 SVQADVATSLVQVANALGANHTTTPTEWVKSLREKDDEKESANAKKMEQKLTNGFLNPLN 456
Query: 483 KLSDLVLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVA 542
L L AI+ G AA + P ++ G GT+G G A+GA+
Sbjct: 457 FLRTLDQSLPDDAILVADGGDFVGSAAYIVRPRGPLQWLDPGAFGTLGVGGGFALGAKTV 516
Query: 543 RPDAIVIDIDGDASFNMTLMELSSAVQARTPV 574
P V I GD S +LME + + + PV
Sbjct: 517 YPKRPVYIIWGDGSCGYSLMEYDTFARHKLPV 548
>SPAC186.09 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 572
Score = 94.4 bits (233), Expect = 5e-19
Identities = 123/555 (22%), Positives = 224/555 (40%), Gaps = 58/555 (10%)
Query: 115 FGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGKPGVVLVTSGPGATNV 174
F PG L + D + ++ V +E AEGYARA+G V VT GA
Sbjct: 33 FVVPGDYNLGLLDKLQYNNYLEEVNCANELNCAFAAEGYARANGIAACV-VTYSVGAFTA 91
Query: 175 VTPMADALADGVPLVVFTGQVATSAIGTDAFQEADV--------VGISRSCTKWNVMVKT 226
+ A A+ +P+++ +G T+ IG+ + +++ T V ++
Sbjct: 92 FDGIGGAYAEDLPVILISGSPNTNDIGSSHLLHHTLGTHDFSYQYEMAKKITCAAVSIQR 151
Query: 227 IEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPMKSILPSDTLNQLTQRVVD 286
E PR I+ A ++A + PV +++P +V + P P I +T NQ
Sbjct: 152 PTEAPRLIDYAIKMALLKKK-PVYIEVPTNVASQPCAAPGPASLITEPETSNQ------- 203
Query: 287 EFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQIPVTTTLQGLGAFN 346
E+ + +A ++N +KP+L G + + +L +L++ V + +F
Sbjct: 204 EYLQMAVDISAKIVNGKQKPVLLAGPKLRSFKAESAFL-ELANSLNCSVAV-MPNAKSFF 261
Query: 347 QEDHKSLDMV--GMHGNAAANLAIQNADLIIALGARFDDRVTLNISKFAPEARKAALEKR 404
E H + + G A I +D II G F D + + P+A
Sbjct: 262 PESHPNYAGIYWGQASTLGAESIINWSDCIICAGTTFTDYSSNGWTSLPPKAN------- 314
Query: 405 GGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLVESVPERPEWSAQVAKWKQEYPY 464
++H ++ ++ G + R+ L L + V +A V ++K+ P
Sbjct: 315 --VLHVDV-----DRVTVSDAEFGGVLLRDF--LHELAKKVKAN---NASVVEYKRIRPE 362
Query: 465 AYQ--KETPGSKIKPQTVISKLSDLVLKSGRPAIVTTGVGQHQMWAAQHWTWKSPRSFIT 522
+ + E P + + + +I ++ +LV + V TG + T F
Sbjct: 363 SLEIPMENPKAALNRKEIIRQVQNLVNQE-TTLFVDTG---DSWFGGMRITLPEKARFEI 418
Query: 523 SGGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNNE 582
G +G+ +P+A G + P V+ GD SF T+ E+S V+ P+ + L+NN
Sbjct: 419 EMQWGHIGWSVPSAFGYAIGAPKRNVVVFVGDGSFQETVQEVSQMVRLNLPIIMFLINNR 478
Query: 583 EQGMVTQWQSLFYEHRYSHTHQLNPDFVKLAEAMG-----LKALRLSK-QEDTDAILKEF 636
+ + H + N D+ + EA K R+ E +AI +
Sbjct: 479 GYTIEVE------IHDGPYNRIKNWDYAAIVEAFNAGEGHAKGFRVGNGHELAEAIRQAK 532
Query: 637 VDYEGPILLEVQIEK 651
+ +GP L+E I++
Sbjct: 533 ENSQGPTLIECNIDQ 547
>YLR134w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 87.8 bits (216), Expect = 5e-17
Identities = 120/506 (23%), Positives = 201/506 (39%), Gaps = 58/506 (11%)
Query: 96 MTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYAR 155
+T G+ E + + N +TVFG PG L + D +Y + +E A + A+GYAR
Sbjct: 4 ITLGKYLFERLSQVNCNTVFGLPGDFNLSLLDKLYEVKGMRWAGNANELNAAYAADGYAR 63
Query: 156 ASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSA----------IGTDAF 205
G ++ T G G + + +A + A+ V ++ G + S+ +G F
Sbjct: 64 IKGM-SCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISSQAKQLLLHHTLGNGDF 122
Query: 206 QEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPK-----DVTAA 260
+S + ++ M+ I P I+ T PV + LP +V A
Sbjct: 123 TVFH--RMSANISETTAMITDIANAPAEIDRCIR-TTYTTQRPVYLGLPANLVDLNVPAK 179
Query: 261 VLRNPIPMKSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMG 320
+L PI + S+ P+D E E + +LI AK P++ A ++
Sbjct: 180 LLETPIDL-SLKPNDA----------EAEAEVVRTVVELIKDAKNPVILADACASRHDVK 228
Query: 321 PQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDM-VGMHGNAAANLAIQNADLIIALGA 379
+ K+L D Q PV T G GA +++ + + VG A+++ADLI+++GA
Sbjct: 229 AE-TKKLMDLTQFPVYVTPMGKGAIDEQHPRYGGVYVGTLSRPEVKKAVESADLILSIGA 287
Query: 380 RFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLL 439
D N F+ K IV F I + L+KLL
Sbjct: 288 LLSD---FNTGSFSYS------YKTKNIVEFHSDHIKIRNATFPGV----QMKFALQKLL 334
Query: 440 PLVESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVTT 499
+ V + + A A+ K TP + Q + L+ G I T
Sbjct: 335 DAIPEVVKDYKPVAVPAR------VPITKSTPANTPMKQEWMWNHLGNFLREGDIVIAET 388
Query: 500 GVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVAR----PDAIVIDIDGDA 555
G + T+ + I G++G+ + A +GA +A P VI GD
Sbjct: 389 GT---SAFGINQTTFPTDVYAIVQVLWGSIGFTVGALLGATMAAEELDPKKRVILFIGDG 445
Query: 556 SFNMTLMELSSAVQARTPVKVLLLNN 581
S +T+ E+S+ ++ + +LNN
Sbjct: 446 SLQLTVQEISTMIRWGLKPYIFVLNN 471
>YLR044c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 87.8 bits (216), Expect = 5e-17
Identities = 125/510 (24%), Positives = 201/510 (38%), Gaps = 64/510 (12%)
Query: 96 MTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYAR 155
+T G+ E +++ NV+TVFG PG L + D IY + + +E A + A+GYAR
Sbjct: 4 ITLGKYLFERLKQVNVNTVFGLPGDFNLSLLDKIYEVEGMRWAGNANELNAAYAADGYAR 63
Query: 156 ASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSA----------IGTDAF 205
G ++ T G G + + +A + A+ V ++ G + SA +G F
Sbjct: 64 IKGM-SCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAKQLLLHHTLGNGDF 122
Query: 206 QEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPK-----DVTAA 260
+S + ++ M+ I P I+ T PV + LP +V A
Sbjct: 123 TVFH--RMSANISETTAMITDIATAPAEIDRCIR-TTYVTQRPVYLGLPANLVDLNVPAK 179
Query: 261 VLRNPIPMKSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMG 320
+L+ PI M S+ P+D E E I L+ AK P++ A ++
Sbjct: 180 LLQTPIDM-SLKPNDA----------ESEKEVIDTILALVKDAKNPVILADACCSRHDVK 228
Query: 321 PQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDM-VGMHGNAAANLAIQNADLIIALGA 379
+ K+L D Q P T G G+ +++ + + VG A+++ADLI+++GA
Sbjct: 229 AE-TKKLIDLTQFPAFVTPMGKGSIDEQHPRYGGVYVGTLSKPEVKEAVESADLILSVGA 287
Query: 380 RFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLL 439
D N F+ K IV F I + L+KLL
Sbjct: 288 LLSD---FNTGSFSYS------YKTKNIVEFHSDHMKIRNATFPGV----QMKFVLQKLL 334
Query: 440 PLVESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDL------VLKSGR 493
+ A AK + P A TP + P + K + L+ G
Sbjct: 335 TTI----------ADAAKGYK--PVAVPARTPANAAVPASTPLKQEWMWNQLGNFLQEGD 382
Query: 494 PAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLG-TMGYGLPAAIGAQVARPDAIVIDID 552
I TG + S + G +G T G L AA A+ P VI
Sbjct: 383 VVIAETGTSAFGINQTTFPNNTYGISQVLWGSIGFTTGATLGAAFAAEEIDPKKRVILFI 442
Query: 553 GDASFNMTLMELSSAVQARTPVKVLLLNNE 582
GD S +T+ E+S+ ++ + +LNN+
Sbjct: 443 GDGSLQLTVQEISTMIRWGLKPYLFVLNND 472
>SPAC1F8.07c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 594
Score = 87.4 bits (215), Expect = 7e-17
Identities = 136/605 (22%), Positives = 239/605 (39%), Gaps = 91/605 (15%)
Query: 96 MTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYAR 155
MT G + + + F PG L + D + + + +E AEGYAR
Sbjct: 9 MTVGTYLAQRLVEIGIKNHFVVPGDYNLRLLDFLEYYPGLSEIGCCNELNCAFAAEGYAR 68
Query: 156 ASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATS----------AIGTDAF 205
++G +VT GA + A A+ +P+++ +G T+ +GT F
Sbjct: 69 SNG-IACAVVTYSVGALTAFDGIGGAYAENLPVILVSGSPNTNDLSSGHLLHHTLGTHDF 127
Query: 206 QEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNP 265
+ + I++ T V +K E+ P I+ A A + PV +++P ++ P
Sbjct: 128 EYQ--MEIAKKLTCAAVAIKRAEDAPVMIDHAIRQAIL-QHKPVYIEIPTNMANQPCPVP 184
Query: 266 IPMKSILPSDTLNQLTQRVVDEFTLENISR-AADLINRAKKPLLYVGAGILGSEMGPQYL 324
P+ ++ ++ + D+ +LE + AA+LI++ +KP+L G + + ++
Sbjct: 185 GPISAV--------ISPEISDKESLEKATDIAAELISKKEKPILLAGPKLRAAGAESAFV 236
Query: 325 KQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMV---GMHGNAAANLAIQNADLIIALGARF 381
K A + F E+HK+ V + + +++DL+I G F
Sbjct: 237 KLA--EALNCAAFIMPAAKGFYSEEHKNYAGVYWGEVSSSETTKAVYESSDLVIGAGVLF 294
Query: 382 DDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNL---EKL 438
+D T+ + AP +P + G V + E L
Sbjct: 295 NDYSTVG-WRAAP------------------NPNILLNSDYTSVSIPGYVFSRVYMAEFL 335
Query: 439 LPLVESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVT 498
L + V ++P AY +K +PQTV+ K ++ R ++
Sbjct: 336 ELLAKKVSKKPATLE-----------AY------NKARPQTVVPKAAEPKAALNRVEVMR 378
Query: 499 TGVG----QHQMWAAQHWTW------KSP--RSFITSGGLGTMGYGLPAAIGAQVARPDA 546
G ++A +W K P F G +G+ +P+A+G VA P+
Sbjct: 379 QIQGLVDSNTTLYAETGDSWFNGLQMKLPAGAKFEVEMQWGHIGWSVPSAMGYAVAAPER 438
Query: 547 IVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNNEEQGMVTQWQSLFYEHRYSHTHQLN 606
I + GD SF +T E+S ++ + PV + LLNN +G + Q H + N
Sbjct: 439 RTIVMVGDGSFQLTGQEISQMIRHKLPVLIFLLNN--RGYTIEIQI----HDGPYNRIQN 492
Query: 607 PDFVKLAEAMG-----LKALRLSKQED-TDAILKEFVDYEGPILLEVQIEKKVPVLPMVP 660
DF E++ K L E+ T AI + EGP L+E I+ +V
Sbjct: 493 WDFAAFCESLNGETGKAKGLHAKTGEELTSAIKVALQNKEGPTLIECAIDTDDCTQELVD 552
Query: 661 TGKGL 665
GK +
Sbjct: 553 WGKAV 557
>YGR087c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 81.3 bits (199), Expect = 5e-15
Identities = 125/510 (24%), Positives = 209/510 (40%), Gaps = 64/510 (12%)
Query: 96 MTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYAR 155
+T G+ E +++ NV+T+FG PG L + D IY D + +E A + A+GYAR
Sbjct: 4 ITLGKYLFERLKQVNVNTIFGLPGDFNLSLLDKIYEVDGLRWAGNANELNAAYAADGYAR 63
Query: 156 ASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSA----------IGTDAF 205
G V++ T G G + + +A + A+ V ++ G + SA +G F
Sbjct: 64 IKGL-SVLVTTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAKQLLLHHTLGNGDF 122
Query: 206 QEADVVGISRSCTKWNVMVKTIEELPRRINEAFEIA-TSGRPGPV-----LVDLPKDVTA 259
+S + ++ M+ I P I+ + RP + LVDL V
Sbjct: 123 TVFH--RMSANISETTSMITDIATAPSEIDRLIRTTFITQRPSYLGLPANLVDL--KVPG 178
Query: 260 AVLRNPIPMKSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEM 319
++L PI + S+ P+D E E I +LI +K P++ A +
Sbjct: 179 SLLEKPIDL-SLKPND----------PEAEKEVIDTVLELIQNSKNPVILSDACASRHNV 227
Query: 320 GPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDM-VGMHGNAAANLAIQNADLIIALG 378
+ ++L D Q P T G G+ +++ + + VG A+++ADLI+++G
Sbjct: 228 KKE-TQKLIDLTQFPAFVTPLGKGSIDEQHPRYGGVYVGTLSKQDVKQAVESADLILSVG 286
Query: 379 ARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKL 438
A D N F+ K +V F + K +NL K+
Sbjct: 287 ALLSD---FNTGSFSYS------YKTKNVVEFHSDYVKV-KNATFLGVQMKFALQNLLKV 336
Query: 439 LPLV----ESVPERPEWSAQVAKWKQEYPYAYQKETPGS-KIKPQTVISKLSDLVLKSGR 493
+P V +SVP + A K P S +K + + ++LS L+ G
Sbjct: 337 IPDVVKGYKSVPVPTKTPA-------------NKGVPASTPLKQEWLWNELSKF-LQEGD 382
Query: 494 PAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLG-TMGYGLPAAIGAQVARPDAIVIDID 552
I TG + S + G +G T G L AA A+ P+ VI
Sbjct: 383 VIISETGTSAFGINQTIFPKDAYGISQVLWGSIGFTTGATLGAAFAAEEIDPNKRVILFI 442
Query: 553 GDASFNMTLMELSSAVQARTPVKVLLLNNE 582
GD S +T+ E+S+ ++ + +LNN+
Sbjct: 443 GDGSLQLTVQEISTMIRWGLKPYLFVLNND 472
>At5g01320 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 603
Score = 70.9 bits (172), Expect = 6e-12
Identities = 104/502 (20%), Positives = 199/502 (38%), Gaps = 53/502 (10%)
Query: 97 TGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARA 156
T G + + V VF PG L + D + + + +E AG+ A+GYAR+
Sbjct: 42 TLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADGYARS 101
Query: 157 SGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADV------ 210
G G +VT G +V+ +A A ++ +P++ G ++ GT+ +
Sbjct: 102 RG-VGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFS 160
Query: 211 --VGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTA----AVLRN 264
+ ++ T + +V +E+ +I++A A PV + + ++ A R+
Sbjct: 161 QELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALK-ESKPVYISISCNLAATPHPTFARD 219
Query: 265 PIPMKSILPSDTLNQLTQRVVDEFTLE-NISRAADLINRAKKPLLYVGAGILGSEMGPQY 323
P+P LT R+ + LE + + +N+A KP++ G + ++ +
Sbjct: 220 PVPF----------DLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAF 269
Query: 324 LKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAA----NLAIQNADLIIALGA 379
L +L+D + P+ G E+H +G + A + + +++AD I G
Sbjct: 270 L-ELADASGYPLAVMPSTKGLV-PENHP--HFIGTYWGAVSTPFCSEIVESADAYIFAGP 325
Query: 380 RFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLL 439
F+D ++ S + KA + +V + D R L K +
Sbjct: 326 IFNDYSSVGYSLLL-KKEKAIIVHPDRVV--------VANGPTFGCVLMSDFFRELAKRV 376
Query: 440 PLVESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGRPAIVTT 499
E+ E E V + K K PG ++ + + + L S I T
Sbjct: 377 KRNETAYENYE-RIFVPEGKP------LKCKPGEPLRVNAMFQHIQKM-LSSETAVIAET 428
Query: 500 GVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPDAIVIDIDGDASFNM 559
G + Q + G++G+ + A +G A P+ V+ GD SF +
Sbjct: 429 G---DSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQV 485
Query: 560 TLMELSSAVQARTPVKVLLLNN 581
T ++S+ ++ + L+NN
Sbjct: 486 TAQDISTMIRNGQKAIIFLINN 507
>YDL080c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 609
Score = 68.9 bits (167), Expect = 2e-11
Identities = 111/522 (21%), Positives = 202/522 (38%), Gaps = 93/522 (17%)
Query: 101 IFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGKP 160
+FH + Q N+ T+FG G +P+ D +YN + +E A + A+GY+R G
Sbjct: 21 LFHRLNQL-NIHTIFGLSGEFSMPLLDKLYNIPNLRWAGNSNELNAAYAADGYSRLKGL- 78
Query: 161 GVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSA----------IGTDAFQEADV 210
G ++ T G G + + +A + A+ V ++ G TSA +G F
Sbjct: 79 GCLITTFGVGELSAINGVAGSYAEHVGILHIVGMPPTSAQTKQLLLHHTLGNGDFTVFHR 138
Query: 211 VGISRSCTKWNVMVKTI--EELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVLRNPIPM 268
+ +C ++ + +E+ + I +A+ PV + +P V + +P+
Sbjct: 139 IASDVACYTTLIIDSELCADEVDKCIKKAWI-----EQRPVYMGMP------VNQVNLPI 187
Query: 269 KSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLS 328
+S + L+ + + E ISR I +++ P + V A + + K+L
Sbjct: 188 ESARLNTPLDLQLHKNDPDVEKEVISRILSFIYKSQNPAIIVDACTSRQNL-IEETKELC 246
Query: 329 DRAQIPVTTTLQGLGAFNQEDHKSLDM-VGMHGNAAANLAIQNADLIIALGARFDDRVTL 387
+R + PV T G G N+ D + + G + AD II +G + T
Sbjct: 247 NRLKFPVFVTPMGKGTVNETDPQFGGVFTGSISAPEVREVVDFADFIIVIGCMLSEFST- 305
Query: 388 NISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEK---------- 437
+ F + + AL + + KN + NL++
Sbjct: 306 STFHFQYKTKNCAL-----LYSTSVKLKNATYPDLSIKLLLQKILANLDESKLSYQPSEQ 360
Query: 438 ---LLPL---VESVPERPEWS-AQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLK 490
++P +V R EW +++ W Q PG I +T S +
Sbjct: 361 PSMMVPRPYPAGNVLLRQEWVWNEISHWFQ----------PGDIIITETGASAFG---VN 407
Query: 491 SGRPAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPDA---- 546
R + T G+ Q +W G++GY + A +GA+ A +
Sbjct: 408 QTRFPVNTLGISQ-ALW-------------------GSVGYTMGACLGAEFAVQEINKDK 447
Query: 547 ------IVIDIDGDASFNMTLMELSSAVQARTPVKVLLLNNE 582
VI GD +F +T+ ELS+ V+ + ++NN+
Sbjct: 448 FPATKHRVILFMGDGAFQLTVQELSTIVKWGLTPYIFVMNNQ 489
>At5g01330 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 592
Score = 64.3 bits (155), Expect = 6e-10
Identities = 111/512 (21%), Positives = 204/512 (39%), Gaps = 73/512 (14%)
Query: 97 TGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARA 156
T G + + V +F PG L + D + + + + +E AG+ A+GYAR+
Sbjct: 31 TLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAADGYARS 90
Query: 157 SGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADV------ 210
G G +VT G +V+ +A A ++ +P++ G ++ GT+ +
Sbjct: 91 RG-VGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFS 149
Query: 211 --VGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPG-PVLVDLPKDVTAAVLRNPIP 267
+ ++ T + +V +E+ +I++A IAT+ R PV + + ++ A IP
Sbjct: 150 QELRCFQTVTCYQAVVNHLEDAHEQIDKA--IATALRESKPVYISISCNLAA------IP 201
Query: 268 MKSILPSDTLNQLTQRVVDEFTLE-NISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQ 326
+ LT R+ ++ LE + + +N+A KP++ VG L +
Sbjct: 202 HPTFASYPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVM-VGGPKLRVAKARDAFVE 260
Query: 327 LSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAANL----AIQNADLIIALGARFD 382
L+D + PV G F E+H +G + A + L +++AD I G F+
Sbjct: 261 LADASGYPVAVMPSAKG-FVPENHP--HFIGTYWGAVSTLFCSEIVESADAYIFAGPIFN 317
Query: 383 DRVTLNISKFAPEARKAALEKRGGIVHFEISPKNINKXXXXXXXXXGDVTRNLEKLLPLV 442
D ++ S L+K I+ + P ++ R E L
Sbjct: 318 DYSSVGYS--------LLLKKEKAII---VHPDSV----VVANGPTFGCVRMSEFFRELA 362
Query: 443 ESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQ-----TVISKLSDLVLKSGRPAIV 497
+ V +P +A + Y + E K KP+ + + +L + I
Sbjct: 363 KRV--KPNKTAY-----ENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIA 415
Query: 498 TTGVGQHQMWAAQHWTWKSPRSFITSGGLG---TMGYG-----LPAAIGAQVARPDAIVI 549
TG +W + + G G M YG + A +G A P+ V+
Sbjct: 416 ETGD-----------SWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVL 464
Query: 550 DIDGDASFNMTLMELSSAVQARTPVKVLLLNN 581
GD SF +T ++S+ ++ + L+NN
Sbjct: 465 SFIGDGSFQVTAQDVSTMIRNGQKTIIFLINN 496
>At4g33070 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 607
Score = 63.9 bits (154), Expect = 8e-10
Identities = 108/508 (21%), Positives = 197/508 (38%), Gaps = 55/508 (10%)
Query: 92 SFIGMTGGEIFHEMMQRHNVDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAE 151
++ T G + + V VF PG L + D + + +E AG+ A+
Sbjct: 41 NYCDATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAAD 100
Query: 152 GYARASGKPGVVLVTSGPGATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADV- 210
GYAR+ G G +VT G +V+ +A A ++ +PL+ G ++ GT+ +
Sbjct: 101 GYARSRG-VGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIG 159
Query: 211 -------VGISRSCTKWNVMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTA---- 259
+ ++ T + +V +++ +I++A A PV + + ++ A
Sbjct: 160 LPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALK-ESKPVYISVSCNLAAIPHH 218
Query: 260 AVLRNPIPMKSILPSDTLNQLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEM 319
R+P+P L N++ E TLE +N+A KP++ VG L
Sbjct: 219 TFSRDPVPFS--LAPRLSNKMGLEAAVEATLE-------FLNKAVKPVM-VGGPKLRVAK 268
Query: 320 GPQYLKQLSDRAQIPVTTTLQGLGAFNQEDHKSLDMVGMHGNAAA----NLAIQNADLII 375
+L+D + + G F E H +G + A + + +++AD I
Sbjct: 269 ACDAFVELADASGYALAMMPSAKG-FVPEHHP--HFIGTYWGAVSTPFCSEIVESADAYI 325
Query: 376 ALGARFDDRVTLNISKFAPEARKAALEKRGGIVHFEISPKNIN--KXXXXXXXXXGDVTR 433
G F+D ++ S L+K IV + P I D R
Sbjct: 326 FAGPIFNDYSSVGYS--------LLLKKEKAIV---VQPDRITVANGPTFGCILMSDFFR 374
Query: 434 NLEKLLPLVESVPERPEWSAQVAKWKQEYPYAYQKETPGSKIKPQTVISKLSDLVLKSGR 493
L K + E+ E V + K P + P ++ T+ + + L S
Sbjct: 375 ELSKRVKRNETAYENYH-RIFVPEGK---PLKCESREP---LRVNTMFQHIQKM-LSSET 426
Query: 494 PAIVTTGVGQHQMWAAQHWTWKSPRSFITSGGLGTMGYGLPAAIGAQVARPDAIVIDIDG 553
I TG + Q + G++G+ + A +G A P+ V+ G
Sbjct: 427 AVIAETG---DSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIG 483
Query: 554 DASFNMTLMELSSAVQARTPVKVLLLNN 581
D SF +T+ ++S+ ++ + L+NN
Sbjct: 484 DGSFQVTVQDISTMLRNGQKTIIFLINN 511
>At5g54960 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 607
Score = 60.8 bits (146), Expect = 7e-09
Identities = 67/296 (22%), Positives = 126/296 (41%), Gaps = 31/296 (10%)
Query: 111 VDTVFGYPGGAILPVYDAIYNSDKFTFVLPKHEQGAGHMAEGYARASGKPGVVLVTSGPG 170
V VF PG L + D + + +E AG+ A+GYAR+ G G +VT G
Sbjct: 60 VTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYARSRG-VGACVVTFTVG 118
Query: 171 ATNVVTPMADALADGVPLVVFTGQVATSAIGTDAFQEADV--------VGISRSCTKWNV 222
+V+ +A A ++ +PL+ G ++ GT+ + + ++ T +
Sbjct: 119 GLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQA 178
Query: 223 MVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAAVL----RNPIPMKSILPSDTLN 278
++ +EE I+ A A PV + + ++ A L R+P+P +LP N
Sbjct: 179 VINNLEEAHELIDTAISTALK-ESKPVYISISCNLPAIPLPTFSRHPVPF--MLPMKVSN 235
Query: 279 QLTQRVVDEFTLENISRAADLINRAKKPLLYVGAGILGSEMGPQYLKQLSDRAQIPVTTT 338
Q+ + AA+ +N+A KP+L G + ++ ++ +L+D + +
Sbjct: 236 QIGLDAA-------VEAAAEFLNKAVKPVLVGGPKMRVAKAADAFV-ELADASGYGLAVM 287
Query: 339 LQGLGAFNQEDHKSLDMVGMHGNAAANL----AIQNADLIIALGARFDDRVTLNIS 390
G E HK +G + A + +++AD + G F+D ++ S
Sbjct: 288 PSAKGQV-PEHHK--HFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYS 340
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,832,342
Number of Sequences: 60738
Number of extensions: 1723200
Number of successful extensions: 4466
Number of sequences better than 1.0e-05: 23
Number of HSP's better than 0.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 4393
Number of HSP's gapped (non-prelim): 39
length of query: 691
length of database: 30,389,216
effective HSP length: 113
effective length of query: 578
effective length of database: 23,525,822
effective search space: 13597925116
effective search space used: 13597925116
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)