ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3219 suspect: LH L KOG2326 Replication, recombination and repair DNA-binding subunit of a DNA-dependent protein kinase (Ku80 autoantigen)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3219 1105330 1107156 609
(609 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR106c [L] KOG2326 DNA-binding subunit of a DNA-dependent prote... 283 4e-76
SPBC543.03c [L] KOG2326 DNA-binding subunit of a DNA-dependent p... 72 2e-12
>YMR106c [L] KOG2326 DNA-binding subunit of a DNA-dependent protein kinase
(Ku80 autoantigen)
Length = 629
Score = 283 bits (725), Expect = 4e-76
Identities = 192/590 (32%), Positives = 309/590 (51%), Gaps = 60/590 (10%)
Query: 2 SELTCFVVDVGS--LSGDQWLKAFTYVEYVLLNKVQSQRKTDYVQLNLVNVSSSDESLPV 59
SE T F+VDV + + K+ Y+EY LLNK + RKTD++ L N S+ S +
Sbjct: 3 SESTTFIVDVSPSMMKNNNVSKSMAYLEYTLLNKSKKSRKTDWISCYLANCPVSENSQEI 62
Query: 60 NNMAMLPVLPRAPISISDVRSW---LGQWYDERNNTTGNGEXXXXXMLFNGLLISVLKLK 116
N+ + AP++ + + L Q+ D+ ++ + N + LL+ L +K
Sbjct: 63 PNVFQIQSF-LAPVTTTATIGFIKRLKQYCDQHSHDSSN---EGLQSMIQCLLVVSLDIK 118
Query: 117 EFVGKRKMKVKIVVFSSQ-DLQNVTDEELSTFADQCPFDLVIADCNDKQGHQESKHGNWL 175
+ RK+ +IVVF+ D ++TDEE+ ++ +++ DC K +E K NWL
Sbjct: 119 QQFQARKILKQIVVFTDNLDDLDITDEEIDLLTEELSTRIILIDCG-KDTQEERKKSNWL 177
Query: 176 KLGTPL----VYGIDELIQSVADPKLKLTRPIRTFQGQLRLG-DVNASDLGIPS------ 224
KL + +Y ++EL+ + P + +P+R F G+LRLG D+ ++ PS
Sbjct: 178 KLVEAIPNSRIYNMNELLVEITSPATSVVKPVRVFSGELRLGADILSTQTSNPSGSMQDE 237
Query: 225 --LCINLEGVPGTKSVSSISRKVMRKEGSSEGQPTYRAVKSVIEYQIEDQSASTSASPKK 282
LCI +E P TK+VS ++RK + S+ + Y VKS+IEY+I ++ + S
Sbjct: 238 NCLCIKVEAFPATKAVSGLNRKTAVEVEDSQKKERYVGVKSIIEYEIHNEGNKKNVS--- 294
Query: 283 ETTRKDDDGDSGNNTGPRMINVSKDFIKKAYRYGADYVDLPMPLDEERKLSEKPGLDIRG 342
+D SG++ P + +SKD + KAYRYGADYV LP L ++ PGLD+RG
Sbjct: 295 ------EDDQSGSSYIP--VTISKDSVTKAYRYGADYVVLPSVLVDQTVYESFPGLDLRG 346
Query: 343 FMDIDKLPRHYLCSESMYLVPDSKNGSKGDFLGFVTLVDSLIKMKRLIIARFVPKFGNEV 402
F++ + LPR++L SES ++ D++ G + D + F LVD +++ +++ +AR+V K +EV
Sbjct: 347 FLNREALPRYFLTSESSFITADTRLGCQSDLMAFSALVDVMLENRKIAVARYVSKKDSEV 406
Query: 403 QMCSLFPVRVH----DKDXXXXXXXXXXXXPMSEDERNSAFPKMCEST-----ELEPE-- 451
MC+L PV + + + P +EDER + FPK+ + T L+ E
Sbjct: 407 NMCALCPVLIEHSNINSEKKFVKSLTLCRLPFAEDERVTDFPKLLDRTTTSGVPLKKETD 466
Query: 452 ----DEAMGQFVDSMNLDNDAQDLPW--YESDQIQKYTDVALEQSSLPLPQREMFRATAR 505
DE M QFVDSM+ D + ++P Y + TD L SL Q E +
Sbjct: 467 GHQIDELMEQFVDSMDTD-ELPEIPLGNYYQPIGEVTTDTTLPLPSLNKDQEE----NKK 521
Query: 506 NPSAIPAISLHRQQQVILECVHQKFIVGKRDQQDLNIPPMSKMILKKITP 555
+P IP + ++RQQQV+LE +HQ I D ++ IP + + KI+P
Sbjct: 522 DPLRIPTVFVYRQQQVLLEWIHQLMI---NDSREFEIPELPDSLKNKISP 568
>SPBC543.03c [L] KOG2326 DNA-binding subunit of a DNA-dependent protein kinase
(Ku80 autoantigen)
Length = 695
Score = 72.4 bits (176), Expect = 2e-12
Identities = 108/498 (21%), Positives = 194/498 (38%), Gaps = 69/498 (13%)
Query: 3 ELTCFVVDVGSLSGD----------QWLKAFTYVEYVLLNKVQSQRKTDYVQLNLVNVSS 52
E T FV+D+G G +W ++ + E L +K + RKTD V +
Sbjct: 5 ECTVFVLDLGKDMGTCHHGRSHSDLEWTLSYFHDE--LSHKFLANRKTDVVGIVGYKCDD 62
Query: 53 SDESLPVN----NMAMLPVLPRAPISISDVRSWLGQWYDERNNTTGNGEXXXXXMLFNGL 108
+ L N+++L P S ++S + Q N G+ L + +
Sbjct: 63 TKNDLAEQEAYWNISVL--YPIQTALFSKLQS-VSQTLKPSNTMQGD--------LISAI 111
Query: 109 LISVLKLKEFVGKRKMKVKIVVFSS-------QDLQNVTDEELS--TFADQCPFDLVIAD 159
++S + K K K K++V ++ D + ++ L F D D
Sbjct: 112 VVSFDLMARHCKKNKWKKKMIVLTAARGIIDFSDYIGIAEQLLQHDVFLGVYGVDFDQED 171
Query: 160 CNDKQGHQES-KHGNWLKLG--TPLVYG----IDELIQSVADPKLKLTRPIRTFQGQLRL 212
N + +ES K N +++ +G ++ ++ P ++ RP+ F+G +
Sbjct: 172 INYSEPLKESQKKENEVRIQEFVESCHGQYCTFQQIYNNIGKPWVRKVRPVAIFRGTFSI 231
Query: 213 GDVNASDLGIPSLCINLEGVPGTKSVSSISRKVMRKEGSSEGQPTYRAVKSVIEYQ-IED 271
G+ ++ D S+ I +E P T+ + + S+ S +E + +E
Sbjct: 232 GNRDSKDT---SISIQVERYPRTRLTKPPTSSAFYENDMSKNYECLNIENSNVENKSMES 288
Query: 272 QSASTSASPKKETTRKDDDGDSGNNTGPRMINVSKDFIKKAYRYGADYVDLPMPLDEERK 331
+ ST S + +D V ++ ++ Y YG V + ++
Sbjct: 289 DAVSTVRSYMVRDPKTNDS-----------FEVKREDLESGYSYGRTIVPISRSDEDVLA 337
Query: 332 LSEKPGLDIRGFMDIDKLPRHYLCSESMYLVPDSKNGSKGDFLGFVTLVDSLIKMKRLII 391
L PG +I GF+ LP +Y S++ +VP SK L F V SL + R +
Sbjct: 338 LDTIPGYEILGFIPKSSLPIYYTISDTNIIVPKDDFESK---LNFSAFVQSLEREHRYAL 394
Query: 392 ARFVPKFGNEVQMCSLFPVRVHDKDXXXXXXXXXXXXPMSEDERN---SAFPKMCESTEL 448
ARFV K + L P + P +ED R S F K+ ++
Sbjct: 395 ARFVSKDKGVPVLLVLMPYVEFKR-----HYLVDIQLPFAEDVRPYSFSEFEKLSNEEDM 449
Query: 449 EPEDEAMGQFVDSMNLDN 466
D A+ ++D+M+LD+
Sbjct: 450 RQIDFAVSNYIDNMDLDS 467
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,918,793
Number of Sequences: 60738
Number of extensions: 1528948
Number of successful extensions: 4144
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4132
Number of HSP's gapped (non-prelim): 3
length of query: 609
length of database: 30,389,216
effective HSP length: 112
effective length of query: 497
effective length of database: 23,586,560
effective search space: 11722520320
effective search space used: 11722520320
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)