ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3289 good R KOG4409 General function prediction only Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3289 1126975 1128252 426
(426 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR110w [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/b... 400 e-111
SPAC6G10.03c [R] KOG4409 Predicted hydrolase/acyltransferase (al... 149 8e-36
At4g24160 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha... 117 3e-26
Hs11545767 [R] KOG4409 Predicted hydrolase/acyltransferase (alph... 95 2e-19
CE08627 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/b... 91 3e-18
7304150 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/b... 84 3e-16
CE08626 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/b... 75 2e-13
Hs7705771 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha... 74 3e-13
CE08378 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/b... 58 3e-08
>YGR110w [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 445
Score = 400 bits (1027), Expect = e-111
Identities = 191/397 (48%), Positives = 264/397 (66%), Gaps = 25/397 (6%)
Query: 54 VRKSTAPIGLPLLQIVTNLHKLFPLSFAESLKDYKQYNINKEQFQEDLLSVLPYYPEPSA 113
+R +AP +PL +I+ + LFP +S+KD++ + N++ FQ +LL LP+YP PS
Sbjct: 46 LRTPSAPTAIPLREIIYRVPSLFPRPLEDSVKDFRDFIKNEDAFQTELLKTLPFYPTPSE 105
Query: 114 RFSSKIIKTPIDAAGNYINEFQITPIRPESDGKPLKHIILVHGYGAGLGFYLKNFN--AL 171
++++I+T +D GNYINEF I P + LKH++ +HGYGAGLGF++KNF L
Sbjct: 106 SKTARLIRTVVDDEGNYINEFCIRPRKTSVPEADLKHLVFIHGYGAGLGFFIKNFEDIPL 165
Query: 172 LSENVVVHAIDLPGYGFSSRPMFPFHYPKDSYLAVEDYFHDALHKWFDKKGLISCWENNY 231
L +HAIDLPGYGFSSRP FPF YP+D+ +V+D+FH+ +H WF K+ L++ E N
Sbjct: 166 LDNEWCIHAIDLPGYGFSSRPKFPFEYPRDNIHSVQDWFHERIHTWFSKRNLLNRPEKNI 225
Query: 232 VIAHSMGAYIFSLYANKYKH---FKKLFMCSPGGISRN---------KDMPPPPWWFLKL 279
V+AHS+G+Y+ +LY KYK FKKL +CSP G+S + PPPWW++KL
Sbjct: 226 VMAHSLGSYLMALYLQKYKESPSFKKLILCSPAGVSYRDFNNTASEVEKWKPPPWWYVKL 285
Query: 280 WDQNISPFSLVRNAGHVGSKLVSGWTARRF-------GLHHPNFDKLHHYTYGIFNQPGS 332
WD+NISPF+LVRN +GSK+ SGW+ RRF F+ LH Y Y IFN+ GS
Sbjct: 286 WDRNISPFTLVRNFRQLGSKITSGWSYRRFKHILNGDPEQSKRFEALHRYAYAIFNKRGS 345
Query: 333 GEYMLSFMLGCGGWPRVPLETRLF---TEENFNSSHLDWVWLYGDSDWMDINGARRINDF 389
GEY+LSF L CGG PR+ LE +LF + +S+ DW+WLYGD DWMD+NG R++ F
Sbjct: 346 GEYLLSFALKCGGEPRLSLEQQLFDGKKSDILKNSNCDWLWLYGDDDWMDVNGGLRVSRF 405
Query: 390 LRKKC-INTKLEIVPNAGHHLYWDNDSFFNNVLRKEL 425
L++K + + IVP++GHHLY DN FFNN+L KE+
Sbjct: 406 LKEKLKQKSNVIIVPHSGHHLYLDNYKFFNNILTKEM 442
>SPAC6G10.03c [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 428
Score = 149 bits (376), Expect = 8e-36
Identities = 105/346 (30%), Positives = 157/346 (45%), Gaps = 69/346 (19%)
Query: 139 IRPESDGKPLKH-IILVHGYGAGLGFYLKNFNAL---LSENVVVHAIDLPGYGFSSRPMF 194
+ ++ GK K I+ +HGYGAGLGFY +N + L ++++ + +D G G SSRP F
Sbjct: 81 VSDKATGKANKRSIVYMHGYGAGLGFYFRNMDGLTKGVTKDFNSYFVDWLGMGNSSRPPF 140
Query: 195 PF--HYPKDSYLAVEDYFHDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYANKY-KH 251
+ E +F ++L W G+ E ++ HSMG Y+ ++YA +Y +
Sbjct: 141 DIKGQTASEKVEETERFFTESLETWRIGHGI----EKMILVGHSMGGYLSAVYAMQYPER 196
Query: 252 FKKLFMCSPGGISRN--------------------------------------------- 266
+KL + SP I N
Sbjct: 197 VEKLLLVSPVAIPENPFASNDDAEVYNSVASSAVHAVMDEPPLSNVTNEVLQTQEETTGL 256
Query: 267 ------KDMPPPPWWFLKLWDQNISPFSLVRNAGHVGSKLVSGWTARRFGLHHPN-FDKL 319
K P P + LW+QN++PFSL+R +G +G KL+S W++RRF P F L
Sbjct: 257 EPSRPSKPKNPLPRFITFLWEQNVTPFSLLRLSGPLGPKLMSFWSSRRFSTLPPETFRAL 316
Query: 320 HHYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETRLFTEENFNSSHLDWVWLYGDSDWMD 379
H+Y Y IF GS EY L +L G + R + RL +++YGD DWMD
Sbjct: 317 HNYCYSIFRLKGSSEYALGNLLAPGAFARRCIMNRL------RMLKCRTIFMYGDKDWMD 370
Query: 380 INGARRINDFLRKKCINTKLEIVPNAGHHLYWDNDSFFNNVLRKEL 425
+ L++ I + I+ NAGHH Y DN FN ++ KE+
Sbjct: 371 DVAGLEATNRLKEMNIEAEHHIISNAGHHCYLDNPEDFNEIVLKEI 416
>At4g24160 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 407
Score = 117 bits (294), Expect = 3e-26
Identities = 86/291 (29%), Positives = 133/291 (45%), Gaps = 40/291 (13%)
Query: 151 IILVHGYGAGLGFYLKNFNALLSENVVVHAIDLPGYGFSSRPMFPFHYPKDSYLAVEDYF 210
+++VHGYGA GF+ +NF+AL S V+ AID G+G SSRP F +++ E +F
Sbjct: 112 LVMVHGYGASQGFFFRNFDALASRFRVI-AIDQLGWGGSSRPDFTCRSTEET----EAWF 166
Query: 211 HDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYANKY-KHFKKLFMCSPGGISRNKDM 269
D+ +W + L N ++ HS G Y+ + YA K+ +H + L + G S D
Sbjct: 167 IDSFEEWRKAQNL----SNFILLGHSFGGYVAAKYALKHPEHVQHLILVGSAGFSAEADA 222
Query: 270 PPPPWWFLK------------LWDQNISPFSLVRNAGHVGSKLVSGWTARRFGLH----- 312
W K LW+ N +P LVR G G LV+ +T RFG H
Sbjct: 223 KSE--WLTKFRATWKGAVLNHLWESNFTPQKLVRGLGPWGPGLVNRYTTARFGAHSEGTG 280
Query: 313 --HPNFDKLHHYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETRLFTEENFNSSHLDWVW 370
L Y Y SGE L ++ G + R PL ++ + + +
Sbjct: 281 LTEEEAKLLTDYVYHTLAAKASGELCLKYIFSFGAFARKPL------LQSASEWKVPTTF 334
Query: 371 LYGDSDWMDINGARRINDFLRKKCINTKLEIVPNAGHHLYWDNDSFFNNVL 421
+YG +DWM+ GA ++ C ++ VP GH ++ DN F++ +
Sbjct: 335 IYGMNDWMNYQGAVEARKSMKVPC---EIIRVPQGGHFVFIDNPIGFHSAV 382
>Hs11545767 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 342
Score = 94.7 bits (234), Expect = 2e-19
Identities = 84/303 (27%), Positives = 134/303 (43%), Gaps = 31/303 (10%)
Query: 132 NEFQITPIRPE-SDGKPLKHIILVHGYGAGLGFYLKNFNALLSENVVVHAIDLPGYGFSS 190
N+ + PE +D PL ++VHG+G G+G ++ N ++L S +H DL G+G SS
Sbjct: 54 NKIWTVTVSPEQNDRTPL---VMVHGFGGGVGLWILNMDSL-SARRTLHTFDLLGFGRSS 109
Query: 191 RPMFPFHYPKDSYLAVEDYFHDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYANKY- 249
RP FP P+ ED F ++ W + G+ S ++ HS+G ++ + Y+ KY
Sbjct: 110 RPAFP-RDPE----GAEDEFVTSIETWRETMGIPSM----ILLGHSLGGFLATSYSIKYP 160
Query: 250 KHFKKLFMCSPGGISRNKDMP----PPPWWFLKLWD--QNISPFSLVRNAGHVGSKLVSG 303
K L + P G P PP W + +P +++R AG G LV
Sbjct: 161 DRVKHLILVDPWGFPLRPTNPSGICAPPAWVKAVASVLGRSNPLAVLRVAGPWGPGLVQR 220
Query: 304 WT---ARRFGLHHPNFDKLHHYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETRLFTEEN 360
+ R+F + D + Y Y Q SGE M+ GW R P+ R+ +
Sbjct: 221 FRPDFKRKFADFFED-DTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERI----H 275
Query: 361 FNSSHLDWVWLYGDSDWMDINGARRINDFLRKKCINTKLEIVPNAGHHLYWDNDSFFNNV 420
+ +YG W+D + +++ R +EI A HH+Y D FN V
Sbjct: 276 LIRKDVPITMIYGSDTWIDTSTGKKVK-MQRPDSYVRDMEI-KGASHHVYADQPHIFNAV 333
Query: 421 LRK 423
+ +
Sbjct: 334 VEE 336
>CE08627 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 444
Score = 91.3 bits (225), Expect = 3e-18
Identities = 78/285 (27%), Positives = 128/285 (44%), Gaps = 43/285 (15%)
Query: 151 IILVHGYGAGLGFYLKNFNALLSENVVVHAIDLPGYGFSSRPMFPFHYPKDSYLAVEDYF 210
I+L+HG+GAG+ + L ++ VHA DLPG+G SSRP F E
Sbjct: 163 IVLIHGFGAGVALWGSAIKRL-AQFQTVHAFDLPGFGRSSRPKF-----SSDPETAETEM 216
Query: 211 HDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYANKY-KHFKKLFMCSPGGI------ 263
D++ +W DK L E ++ HS G Y+ + YA KY K + L + P G
Sbjct: 217 IDSIEQWRDKMNL----EKMNLVGHSFGGYLATSYALKYPKRVENLILADPWGFNEMDPE 272
Query: 264 ------SRNKDMPPPPWWFLKLWDQNISPFSLVRNAGHVGSKLVSGWTARRFGLHHPNFD 317
SR K++ +W + Q +P +++R G G LV R L +
Sbjct: 273 FAQKLTSRQKNI----FWVI----QQFNPLAVLRLVGGYGPSLV---RRLRPDLALKYSE 321
Query: 318 KLHHYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETRLFTEENFNSSHLDWVWLYGDSDW 377
++ Y Y ++ +GE + + GW + P+ R +N + +++G+ W
Sbjct: 322 DVYDYIYLANSRDPTGEEVFKCLSENLGWAKQPMSKRFHELDN----TVPVTFIHGERSW 377
Query: 378 MDINGARRINDFLRKKCINTKLEIVPNAGHHLYWDN-DSFFNNVL 421
+D RR+ L + + I+ +AGHH+Y D+ D F V+
Sbjct: 378 IDWRTTRRLFGELE----HVESHIMDSAGHHVYADDADKFVQLVI 418
>7304150 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 454
Score = 84.3 bits (207), Expect = 3e-16
Identities = 76/285 (26%), Positives = 127/285 (43%), Gaps = 29/285 (10%)
Query: 151 IILVHGYGAGLGFYLKNFNALLSENVVVHAIDLPGYGFSSRPMFPFHYPKDSYLAVEDYF 210
++L+HG GAG+ ++ N +A ++ V+A+D+ G+G SSRP+F KD+ L E F
Sbjct: 115 LVLLHGLGAGIALWVMNLDAF-AKGRPVYAMDILGFGRSSRPLFA----KDA-LVCEKQF 168
Query: 211 HDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYANKY-KHFKKLFMCSPGGISRNKDM 269
++ +W + + + ++ HSMG +I S YA + + K L + P G K
Sbjct: 169 VKSVEEWRREMNI----NDMILLGHSMGGFIASSYALSHPERVKHLILADPWGFPE-KPS 223
Query: 270 PPPPWWFLKLWDQNIS-------PFSLVRNAGHVGSKLVSGWTA---RRF-GLHHPNFDK 318
+ LW + I+ P +R AG G +V R+F + +
Sbjct: 224 DSTNGKTIPLWVRAIARVLTPLNPLWALRAAGPFGQWVVQKTRPDIMRKFQSTIEEDINL 283
Query: 319 LHHYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETRLFTEENFNSSHLDWVWLYGDSDWM 378
L Y + Q SGE M+ GW + P+ R+ S + ++YG W+
Sbjct: 284 LPQYIHQCNAQNPSGESAFHTMMQSFGWAKHPMIHRIKDVR----SDIPITFIYGSRSWI 339
Query: 379 DINGARRINDFLRKKCINTKLEIVPNAGHHLYWDNDSFFNNVLRK 423
D + +I ++ K IV AGHH+Y D FN + +
Sbjct: 340 DSSSGEKIKSQRGSNMVDIK--IVTGAGHHVYADKPDVFNRYVNE 382
>CE08626 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 372
Score = 75.1 bits (183), Expect = 2e-13
Identities = 77/314 (24%), Positives = 131/314 (41%), Gaps = 38/314 (12%)
Query: 114 RFSSKIIKTPIDAAGNYINEFQITPIRPESDGKPLKHIILVHGYGAGLGFYLKNFNALLS 173
+FSS++++ P IN + S+ K I+L+HG+GAG+ + L +
Sbjct: 56 KFSSELVQIPFK--NTEINTITVNCENESSELKSKYPIVLIHGFGAGVALWGSAIKRL-A 112
Query: 174 ENVVVHAIDLPGYGFSSRPMFPFHYPKDSYLAVEDYFHDALHKWFDKKGLISCWENNYVI 233
+ V+AIDLPG+G SSR F D A ++ +A+ +W K L E ++
Sbjct: 113 QFQNVYAIDLPGFGRSSRTKF----STDPETAEKEMI-EAIEQWRVKMNL----EKMNLV 163
Query: 234 AHSMGAYIFSLYANKY-KHFKKLFMCSPGGIS---------RNKDMPPPPWWFLKLWDQN 283
HS G Y+ + YA KY K + L + P G + K W LK
Sbjct: 164 GHSFGGYLSTSYALKYPKRIENLILADPWGFTDVDPSFLEKLTKRQKALFWVILK----- 218
Query: 284 ISPFSLVRNAGHVGSKLVSGWTARRFGLHHPNFDKLHHYTYGIFNQPGSGEYMLSFMLGC 343
+P + +R G G L+ R L + ++ Y Y + +GE + +
Sbjct: 219 FNPLAALRLVGGYGPSLMK---RLRPDLEQKYSEDVYDYIYLANSGNPTGEIIFKSLSEN 275
Query: 344 GGWPRVPLETRLFTEENFNSSHLDWVWLYGDSDWMDINGARRINDFLRKKCINTKLEIVP 403
W + P+ R + + +++G W+D R + + + + I+
Sbjct: 276 LRWAKNPMSKRFHELD----KTVPVKFIHGGMSWVDWKTTREMFGSMD----HVESHIIE 327
Query: 404 NAGHHLYWDNDSFF 417
AGHH+Y D+ F
Sbjct: 328 GAGHHVYADDTDRF 341
>Hs7705771 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 349
Score = 74.3 bits (181), Expect = 3e-13
Identities = 75/285 (26%), Positives = 120/285 (41%), Gaps = 31/285 (10%)
Query: 151 IILVHGYGAGLGFYLKNFNALLSENVVVHAIDLPGYGFSSRPMFPFHYPKDSYLAVEDYF 210
++L+HG+G GLG + NF L + N V+A DL G+G SSRP F + VE+ F
Sbjct: 78 LVLLHGFGGGLGLWALNFGDLCT-NRPVYAFDLLGFGRSSRPRFDSDAEE-----VENQF 131
Query: 211 HDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYANKY-KHFKKLFMCSPGGISRNKDM 269
+++ +W GL + ++ H++G ++ + Y+ KY L + P G D+
Sbjct: 132 VESIEEWRCALGL----DKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDL 187
Query: 270 P----PPPWWFLKLWD--QNISPFSLVRNAGHVGSKLVSGWT---ARRFGLHHPNFDKLH 320
P P W L +P + +R AG G LV R++ + D +
Sbjct: 188 ADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFED-DTVT 246
Query: 321 HYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETRLFTEENFNSSHLD--WVWLYGDSDWM 378
Y Y Q SGE M GW + P+ R+ H D ++G +
Sbjct: 247 EYIYHCNVQTPSGETASKNMTIPYGWAKRPMLQRI------GKMHPDIPVSVIFGARSCI 300
Query: 379 DINGARRINDFLRKKCINTKLEIVPNAGHHLYWDNDSFFNNVLRK 423
D N I + T + AGH++Y D FN +++
Sbjct: 301 DGNSGTSIQSLRPHSYVKT--IAILGAGHYVYADQPEEFNQKVKE 343
>CE08378 [R] KOG4409 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 328
Score = 57.8 bits (138), Expect = 3e-08
Identities = 64/234 (27%), Positives = 97/234 (41%), Gaps = 25/234 (10%)
Query: 133 EFQITPIRP-ESDGKPLKHIILVHGYGAGLGFYLKNFNALLSENVVVHAIDLPGYGFSSR 191
E +RP E++ + I+ + G GAG+ + NFN+ ++N VH+ D G+G SSR
Sbjct: 98 EVYTVTVRPREAENLNGEAIVFIPGLGAGVAMFTANFNS-CAKNHAVHSFDPLGFGRSSR 156
Query: 192 PMFPFHYPKDSYLAVEDYFHDALHKWFDKKGLISCWENNYVIAHSMGAYIFSLYA-NKYK 250
F D E + + W G+ E Y+I H+ G Y+ S YA
Sbjct: 157 SRF-----SDDNAIAELEMVEVMEDWRKAMGI----EKMYIIGHAFGGYLASAYALENPS 207
Query: 251 HFKKLFMCSPGGISR-----NKDMPPPPWW-FLKLWDQNISPFSLVRNAG----HVGSKL 300
L + P G + K + P W FL +PFS +R G + KL
Sbjct: 208 RVAHLILVDPWGFAEKVETTEKLIKPYAWMSFLGGVAGYFNPFSPMRWMGPYAPAIVKKL 267
Query: 301 VSGWTARRFGLHHPNFDKLHHYTYGIFNQPGSGEYMLSFMLGCGGWPRVPLETR 354
R GLH ++D ++ Y Y + +GE M GW + P+ R
Sbjct: 268 RPDLLLRFPGLH--DYD-IYKYVYYLNLPNPTGETAFMNMTLPVGWAKRPMIKR 318
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.141 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,130,778
Number of Sequences: 60738
Number of extensions: 1384761
Number of successful extensions: 2777
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2740
Number of HSP's gapped (non-prelim): 10
length of query: 426
length of database: 30,389,216
effective HSP length: 109
effective length of query: 317
effective length of database: 23,768,774
effective search space: 7534701358
effective search space used: 7534701358
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)