ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3347 good R KOG1432 General function prediction only Predicted DNA repair exonuclease SIA1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3347 1149114 1150808 565
(565 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR361c [R] KOG1432 Predicted DNA repair exonuclease SIA1 461 e-129
At5g63140 [R] KOG1432 Predicted DNA repair exonuclease SIA1 162 1e-39
SPCC1020.05 [R] KOG1432 Predicted DNA repair exonuclease SIA1 157 3e-38
At5g57140 [R] KOG1432 Predicted DNA repair exonuclease SIA1 137 4e-32
At2g46880 [R] KOG1432 Predicted DNA repair exonuclease SIA1 130 4e-30
YOR137c [R] KOG1432 Predicted DNA repair exonuclease SIA1 97 8e-20
At3g10150 [R] KOG1432 Predicted DNA repair exonuclease SIA1 67 6e-11
>YLR361c [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 578
Score = 461 bits (1187), Expect = e-129
Identities = 253/523 (48%), Positives = 319/523 (60%), Gaps = 26/523 (4%)
Query: 53 VVINFGTLSCFHVGTWFRICPVTESSLKVDLHDS-SLTSRKVITKDLFNSEGYHWFGKSQ 111
+V+N G CFH+G ++ C LK L D RK I KDL S G WFGKS+
Sbjct: 66 LVVNIGYDECFHIGRFYEGC-FNRHELKSTLTDGHQYLQRKRIHKDLRGSFGRRWFGKSE 124
Query: 112 FLWYDVLELKSV----TDVRKLPSGDSVYGVTRISGDLKDCKHPFKNICIHIDELNESAL 167
+L+YDVL V +++ KL +V VT IS KD PF ++ I + ++ L
Sbjct: 125 YLYYDVLYPALVDYFGSNLEKL----NVEAVTGISKYPKDKSLPFMDVSITFEPISIELL 180
Query: 168 ENTRFLSGFSVLFGEDVTEPRIDWDLDKRFTVTNVRY--PSYVTATYWNNDVWKPEVPLL 225
+ ++S ++LFG D +P +W L K F + RY P+Y+T + L
Sbjct: 181 QKRSYISDINILFGVDCIQPIANWTLQKEFPLVKYRYSEPAYLTYKFVGTRPVDTGAQRL 240
Query: 226 FVNDQHKFKIVQLADLHFSVGKGVCRDEFPQHETCEADPKTLQFIDQVLDIEKPQMVVFT 285
D+ KFKIVQLADLH VG+ C DE+P+HE C+ADPKT F+ QVLDIEKPQ+VVFT
Sbjct: 241 QETDEGKFKIVQLADLHLGVGESECIDEYPKHEACKADPKTETFVQQVLDIEKPQLVVFT 300
Query: 286 GDQIMGDECKQDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFASKLPYSL 345
GDQIMGD QDSET LLK +APVI+RKIPWAMVWGNHDDEGSL RWQLSE AS LPYSL
Sbjct: 301 GDQIMGDRSIQDSETVLLKAVAPVIARKIPWAMVWGNHDDEGSLTRWQLSEIASVLPYSL 360
Query: 346 FEIGPRDSKDNQFGLGNYVREVKGGDGTTNI--ALYFLDSHKYSK-SKAFPGYDWVKXXX 402
F+ P D+ DN FG+GNY+ ++ + T LYFLDSHKYS K +PGYDW+K
Sbjct: 361 FKFSPHDTHDNTFGVGNYIYQIFSNNDTEVPVGTLYFLDSHKYSTVGKIYPGYDWIKESQ 420
Query: 403 XXXXXXXXXSHDSIKQAKHSGDLISMAFFHIPLPEYRNFPQES---GSNRVVGTYKEGIT 459
HD + K +SMAFFHIPLPEY N ++ N ++G YKEG+T
Sbjct: 421 WKYIEDY---HDVNLKFKTG---LSMAFFHIPLPEYLNIESKTHPGEKNPLIGMYKEGVT 474
Query: 460 APRYNSEGVKTLHKLGVSVTSVGHDHCNDYCLLDDFNDGEDKIWLCXXXXXXXXXXXXXX 519
AP+YNSEG+ TL +L V V S GHDHCNDYCL DD +KIWLC
Sbjct: 475 APKYNSEGITTLDRLSVDVVSCGHDHCNDYCLRDD--STPNKIWLCYGGGGGEGGYAGYG 532
Query: 520 XTERRIRVYEIDALKKDIYSWKRLNGSPENTFDHQKLISDGVP 562
TERRIR+YEI+ + +I++WKRLNGSP+ FD Q ++ P
Sbjct: 533 GTERRIRIYEINVNENNIHTWKRLNGSPKEIFDFQSMLDGNSP 575
>At5g63140 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 389
Score = 162 bits (410), Expect = 1e-39
Identities = 114/344 (33%), Positives = 157/344 (45%), Gaps = 30/344 (8%)
Query: 232 KFKIVQLADLHFSVG-KGVCRDEFPQHETCEADPKTLQFIDQVLDIEKPQMVVFTGDQIM 290
+FKI+Q+AD+HF+ G K C++ P +D T F+ +V+ EKP ++VFTGD I
Sbjct: 46 EFKILQVADMHFANGAKTQCQNVLPSQRAHCSDLNTTIFMSRVIAAEKPDLIVFTGDNIF 105
Query: 291 GDECKQDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFASKLPYSLFEIGP 350
G + K D+ ++ AP I+ KIPW + GNHD E + R Q+ KLP +L ++ P
Sbjct: 106 GFDVK-DALKSINAAFAPAIASKIPWVAILGNHDQESTFTRQQVMNHIVKLPNTLSQVNP 164
Query: 351 RDSKDNQFGLGNYVREVKGG-----DGTTNIALYFLDSHKYSKSKAFPGYDWVKXXX--- 402
++ G GNY ++ G + + LYFLDS YS GYDW+K
Sbjct: 165 PEAAHYIDGFGNYNLQIHGAADSKLQNKSVLNLYFLDSGDYSSVPYMEGYDWIKTSQQFW 224
Query: 403 XXXXXXXXXSHDSIKQAKHSGDLISMAFFHIPLPEYRNFPQESGSNRVVGTYKEGITAPR 462
+ K G +A+FHIPLPE+ +F N G +EG +A
Sbjct: 225 FDRTSKRLKREYNAKPNPQEGIAPGLAYFHIPLPEFLSF---DSKNATKGVRQEGTSAAS 281
Query: 463 YNSEGVKTLHKLG-VSVTSVGHDHCNDYCLLDDFNDGEDKIWLCXXXXXXXXXXXXXXXT 521
NS TL G V VGHDH ND+C GE K
Sbjct: 282 TNSGFFTTLIARGDVKSVFVGHDHVNDFC-------GELKGLNLCYGGGFGYHAYGKAGW 334
Query: 522 ERRIRVYEIDALK---------KDIYSWKRLNGSPENTFDHQKL 556
ERR RV +D K K I +WKRL+ + D Q L
Sbjct: 335 ERRARVVVVDLNKKRKGKWGAVKSIKTWKRLDDKHLSVIDSQVL 378
>SPCC1020.05 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 509
Score = 157 bits (398), Expect = 3e-38
Identities = 119/390 (30%), Positives = 187/390 (47%), Gaps = 49/390 (12%)
Query: 176 FSVLFGEDVTEPRIDWD--------LDKRFTVTNVRYPSYVTATYWNNDVWKPE---VPL 224
F+ L GEDV E R W L + +T + P T + + E V L
Sbjct: 141 FNTLLGEDVVELREGWSAVLNDFIYLGQPVLLT--QRPCETPPTPSIEALKRKELSSVTL 198
Query: 225 LFVNDQHK-FKIVQLADLHFSVGKGVCRDEFPQH--ETCEADPKTLQFIDQVLDIEKPQM 281
+ +++ K KI+QL+DLH+S CRD +P E C AD KT F++++L +E+P
Sbjct: 199 TYDDEEKKTIKILQLSDLHYSNSDRPCRDPYPYETAEDCMADAKTTAFVNELLQLEEPDF 258
Query: 282 VVFTGDQIMGDECKQDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFASKL 341
V+ TGD I GD + D+ ++L+K ++P + +P+A+ +GNHDD G L+R +L++ S++
Sbjct: 259 VLLTGDLINGDTSR-DARSSLMKAVSPFVDYNVPFAVNFGNHDDLGDLSREELAKILSQI 317
Query: 342 PYSLFEIGPRDSKDNQFGLGNYVREVKGGDGTTNIALYFLDSH-KYSKSKAFPGYDWVKX 400
P S+ IG N G+GN+V A+Y LD+ S + PGYD +
Sbjct: 318 PGSMGLIG------NVSGVGNFVLH-----SPRKFAIYVLDTKGDTSNRRLCPGYDAITE 366
Query: 401 XXXXXXXXXXXSHDSIKQAKHSGDLISMAFFHIPLPEYRNFPQESGSNRVVGTYKEGITA 460
S K A + I MA HIPL E+ + +VG ++E +
Sbjct: 367 DQLEWL--------SSKVADFKYEPIQMAVLHIPLKEF------CETEDLVGAFREPCSY 412
Query: 461 PRYNSEGVKTLHKLGVSVTSVGHDHCNDYC-LLDDFNDGEDKIWLCXXXXXXXXXXXXXX 519
+ K L L + + GHDH ND+C + D+N + C
Sbjct: 413 SICDPNTAKALKSLRIPLAIAGHDHVNDFCGIHPDYN-----TYFCFAGGAGFGGYGGHG 467
Query: 520 XTERRIRVYEIDALKKDIYSWKRLNGSPEN 549
RR RV+E+D +++ + +WKRL PE+
Sbjct: 468 GYVRRARVFELDPVERAVRTWKRLEWPPED 497
>At5g57140 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 397
Score = 137 bits (345), Expect = 4e-32
Identities = 112/360 (31%), Positives = 159/360 (44%), Gaps = 40/360 (11%)
Query: 220 PEVPLLFVNDQHKFKIVQLADLHFSVGKGV-CRDEFPQHETCEADPKTLQFIDQVLDIEK 278
P +PL F D FKI+Q+AD+HF +G CRD +D T +F+ ++++ E+
Sbjct: 46 PNLPLRF-RDDGTFKILQVADMHFGMGMITRCRDVLDSEFEYCSDLNTTRFLRRMIESER 104
Query: 279 PQMVVFTGDQIMGDECKQDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFA 338
P ++ FTGD I G D+ +LL+ + P I IPWA V GNHD E +LNR +L F
Sbjct: 105 PDLIAFTGDNIFGSSTT-DAAESLLEAIGPAIEYGIPWAAVLGNHDHESTLNRLELMTFL 163
Query: 339 SKLPYSLFEIGPRDSKDNQ-------FGLGNYVREVKGGDG-----TTNIALYFLDSHKY 386
S + +S+ +I P + + G GNY V G G +T L+F DS
Sbjct: 164 SLMDFSVSQINPLVEDETKGDTMRLIDGFGNYRVRVYGAPGSVLANSTVFDLFFFDSGDR 223
Query: 387 SKSKAFPGYDWVKXXXXXXXXXXXXSHDSIKQAKHSGDLISMAFFHIPLPEYRNFPQESG 446
+ Y W+K S Q H ++AFFHIP+ E R E
Sbjct: 224 EIVQGKRTYGWIKESQLRWLQDTSIQGHS--QRIHVNP-PALAFFHIPILEVR----ELW 276
Query: 447 SNRVVGTYKEGITAPRYNSEGVKTLHKLG-VSVTSVGHDHCNDYCLLDDFNDGEDK-IWL 504
+G ++EG+ S ++T +G V +GHDH ND+C G K +W
Sbjct: 277 YTPFIGQFQEGVACSIVQSGVLQTFVSMGNVKAAFMGHDHVNDFC-------GTLKGVWF 329
Query: 505 CXXXXXXXXXXXXXXXTERRIRVYEIDALK--------KDIYSWKRLNGSPENTFDHQKL 556
C RR RV E K K I +WKRL+ + D Q L
Sbjct: 330 C-YGGGFGYHAYGRPNWHRRARVIEAKLGKGRDTWEGIKLIKTWKRLDDEYLSKIDEQVL 388
>At2g46880 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 437
Score = 130 bits (328), Expect = 4e-30
Identities = 104/359 (28%), Positives = 155/359 (42%), Gaps = 35/359 (9%)
Query: 228 NDQHKFKIVQLADLHFSVGKGV-CRDEFPQHETCEADPKTLQFIDQVLDIEKPQMVVFTG 286
N +FKI+Q++D+H+ GK C D P +D T F+ + + EKP ++VF+G
Sbjct: 41 NTDGRFKILQVSDMHYGFGKETQCSDVSPAEFPYCSDLNTTSFLQRTIASEKPDLIVFSG 100
Query: 287 DQIMGDECKQDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFASKLPYSLF 346
D + G D ++ AP I IPW + GNHD E + R + ++ KLP SL
Sbjct: 101 DNVYGLCETSDVAKSMDMAFAPAIESGIPWVAILGNHDQESDMTRETMMKYIMKLPNSLS 160
Query: 347 EIGPRDSKDNQF-GLGNYVREVKGGDGT-----TNIALYFLDSHKYSKSKAFP-GYDWVK 399
++ P D+ Q G GNY +++G G+ + + LY LD Y+K F YDWVK
Sbjct: 161 QVNPPDAWLYQIDGFGNYNLQIEGPFGSPLFFKSILNLYLLDGGSYTKLDGFGYKYDWVK 220
Query: 400 XXXXXXXXXXXXSHDSIKQA---KHSGDLISMAFFHIPLPEYRNFPQESGSNRVVGTYKE 456
+ + + + + HIP+PE+ F + S + G +E
Sbjct: 221 TSQQNWYEHTSKWLEMEHKRWPFPQNSTAPGLVYLHIPMPEFALF---NKSTEMTGVRQE 277
Query: 457 GITAPRYNSEGVKTLHKLG-VSVTSVGHDHCNDYCL-LDDFNDGEDKIWLCXXXXXXXXX 514
+P NS L + G V GHDH ND+C L N LC
Sbjct: 278 STCSPPINSGFFTKLVERGEVKGVFSGHDHVNDFCAELHGIN-------LCYAGGAGYHG 330
Query: 515 XXXXXXTERRIRVYEIDALKKDIY----------SWKRLNGSPENTFDHQKLISDGVPY 563
RR+RV E L+K +Y +WKRL+ + D Q L + Y
Sbjct: 331 YGQVGWA-RRVRVVEAQ-LEKTMYGRWGAVDTIKTWKRLDDKNHSLIDTQLLWTKNTKY 387
>YOR137c [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 622
Score = 96.7 bits (239), Expect = 8e-20
Identities = 89/345 (25%), Positives = 137/345 (38%), Gaps = 48/345 (13%)
Query: 226 FVNDQHKFKIVQLADLHFSVGKGVCRDEFPQHETCEADPKTLQFIDQVLDIEKPQMVVFT 285
F Q FKI+Q+ D HF C D T + KT+ FID+VL E P +VV T
Sbjct: 303 FSRGQRSFKILQITDFHFK-----CTDN---SMTVINEIKTVNFIDRVLASENPDLVVIT 354
Query: 286 GDQIMGDECKQDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFASKLPYSL 345
GD ++ D +T ++KV+ P+IS KIP+A+ G D+ Q+ +F LPY+
Sbjct: 355 GD-LLDSHNTIDYQTCIMKVVQPMISNKIPYAISLGVSDESNLATSAQIRDFIRNLPYTF 413
Query: 346 FEIGPRDSKDNQFGLGNYVREVKGGDGTTNIALYFLDSHKYSKSKAFPGYDWVKXXXXXX 405
+ + G+ EV T L D ++ +V
Sbjct: 414 NNVASEE--------GHMAIEVSFKKKLTKNTLLERDIDTEDETNPSEALFFVFDSFAPV 465
Query: 406 XXXXXXSHDSIKQAKHSGDLISMAFFHIPLPEYRNFPQESGSNRVVGTYKEGIT----AP 461
+D I + +AF + PL EYR G ++G Y E T P
Sbjct: 466 NNFLQDYNDLIGKID-----FGLAFQYFPLSEYR----PHGLFPIIGQYNERSTLTVDTP 516
Query: 462 RYNSEGVKT------------LHKLGVSVTSVGHDHCNDYCLLDDFNDGEDKIWLCXXXX 509
R + T L + S GH+H ND CL ++++WLC
Sbjct: 517 RSRGQVSMTINGKHYKSFLDILSLWNIKGVSCGHEHNNDCCL-----QSKNEMWLCYGGS 571
Query: 510 XXXXXXXXXXXTERRIRVYEIDALKKDIYSWKRLNGSPENTFDHQ 554
+R++ +D + +I SWKR + + +D+Q
Sbjct: 572 AGIGLPRIQGIYP-TVRLFNLDDILDEITSWKRNSNLVDEVYDYQ 615
>At3g10150 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 367
Score = 67.4 bits (163), Expect = 6e-11
Identities = 91/363 (25%), Positives = 140/363 (38%), Gaps = 80/363 (22%)
Query: 225 LFVNDQHKFKIVQLADLHFSVGKGVCRDEFPQHETCEADPKTLQFIDQVLDIEKPQMVVF 284
L V + FKI ADLHF G+ D P D ++ + VLD E P VV+
Sbjct: 27 LRVREGSPFKIAIFADLHF--GEDTWTDWGPGQ-----DVNSVNVMSAVLDAETPDFVVY 79
Query: 285 TGDQIMGDECK-QDSETALLKVLAPVISRKIPWAMVWGNHDDEGSLNRWQLSEFASK--- 340
GD + + Q++ K ++P R IPWA ++GNHDD + W L +S
Sbjct: 80 LGDVVTANNIAIQNASLFWDKAISPTRDRGIPWATLFGNHDDASFV--WPLDWLSSSGIP 137
Query: 341 ---------------------------------LPYSLFEIGPRDSKDNQFGLGNYVREV 367
L YS+ I P++ + + NYV V
Sbjct: 138 PLRCPAASDDDGCTFRGTTRVELIQEEIKSSNALSYSM--ISPKELWPS---VSNYVLLV 192
Query: 368 KGGDGTTN--IALYFLDSH--KYSKSKAFPGYDWVKXXXXXXXXXXXXSHDSIKQAKHSG 423
+ D + LYFLDS Y + + +W K +++
Sbjct: 193 ESSDHSKPPVALLYFLDSGGGSYPEVISNAQVEWFKTKS-----------NTLNPYLRIP 241
Query: 424 DLISMAFFHIPLPEYRNF-PQESGSNRVVGTY-KEGITAPRYNSEGVKTL-HKLGVSVTS 480
+LI F+HIP Y+ P+ + VG+ KE + A + ++ L ++ V
Sbjct: 242 ELI---FWHIPSKAYKKVAPRLWITKPCVGSINKEKVVAQEAENGMMRVLENRSSVKAVF 298
Query: 481 VGHDHCNDYCLLDDFNDGEDKIWLCXXXXXXXXXXXXXXXTERRIRVYEIDALKKDIYSW 540
VGH+H D+C +DK+WLC R R+ EI + I +W
Sbjct: 299 VGHNHGLDWCC-----PYKDKLWLC---FARHTGYGGYGNWPRGSRILEISEMPFRIKTW 350
Query: 541 KRL 543
R+
Sbjct: 351 IRM 353
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,178,168
Number of Sequences: 60738
Number of extensions: 1440741
Number of successful extensions: 2666
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2630
Number of HSP's gapped (non-prelim): 10
length of query: 565
length of database: 30,389,216
effective HSP length: 112
effective length of query: 453
effective length of database: 23,586,560
effective search space: 10684711680
effective search space used: 10684711680
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)