ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3396 good P KOG0635 Inorganic ion transport and metabolism Adenosine 5'-phosphosulfate kinase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3396 1169757 1170350 198
(198 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL001c [P] KOG0635 Adenosine 5'-phosphosulfate kinase 358 3e-99
SPAC1782.11 [P] KOG0635 Adenosine 5'-phosphosulfate kinase 276 2e-74
Hs20127475 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine 5'... 211 7e-55
7293733 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine 5'-ph... 208 3e-54
At2g14750 [P] KOG0635 Adenosine 5'-phosphosulfate kinase 206 1e-53
Hs4758880 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine 5'-... 200 9e-52
Hs18574866 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine 5'... 200 9e-52
At5g67520 [P] KOG0635 Adenosine 5'-phosphosulfate kinase 200 1e-51
At4g39940 [P] KOG0635 Adenosine 5'-phosphosulfate kinase 196 1e-50
At3g03900 [P] KOG0635 Adenosine 5'-phosphosulfate kinase 195 4e-50
CE06447 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine 5'-ph... 189 2e-48
>YKL001c [P] KOG0635 Adenosine 5'-phosphosulfate kinase
Length = 202
Score = 358 bits (919), Expect = 3e-99
Identities = 174/198 (87%), Positives = 186/198 (93%)
Query: 1 MATNITWHQNLTYDERKELRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDG 60
MATNITWH NLTYDERK LRKQ GCTIWLTGLSASGKSTIACALEQLLL K LSAYRLDG
Sbjct: 1 MATNITWHPNLTYDERKALRKQDGCTIWLTGLSASGKSTIACALEQLLLQKNLSAYRLDG 60
Query: 61 DNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELHKE 120
DNIRFGLNKDLGFSE+DRNENIRRISEVSKLFADSC+I+ITSFISPYR+DRD+ARELHKE
Sbjct: 61 DNIRFGLNKDLGFSEKDRNENIRRISEVSKLFADSCAISITSFISPYRVDRDRARELHKE 120
Query: 121 GALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQQS 180
LKFIE+FVDVPLEVAEQRDPKGLYKKAREG+IKEFTGISAPYE P +PELHLRTDQ++
Sbjct: 121 AGLKFIEIFVDVPLEVAEQRDPKGLYKKAREGVIKEFTGISAPYEAPKAPELHLRTDQKT 180
Query: 181 VEECALKIYEYLVKEKLI 198
VEECA IYEYL+ EK+I
Sbjct: 181 VEECATIIYEYLISEKII 198
>SPAC1782.11 [P] KOG0635 Adenosine 5'-phosphosulfate kinase
Length = 202
Score = 276 bits (705), Expect = 2e-74
Identities = 136/199 (68%), Positives = 164/199 (82%), Gaps = 1/199 (0%)
Query: 1 MATNITWHQ-NLTYDERKELRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLD 59
MATNIT+H ++T +ER + G TIW+TGLSASGKSTIACALEQ LL +G++ YRLD
Sbjct: 1 MATNITFHPGSVTKEERIKFVGHPGMTIWMTGLSASGKSTIACALEQYLLQRGVTTYRLD 60
Query: 60 GDNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELHK 119
GDN+RFGLN DLGFSEQDRNENIRRI V+KLFAD+C +A+TSFISPYR DRD+ARE HK
Sbjct: 61 GDNVRFGLNSDLGFSEQDRNENIRRIGHVAKLFADACVVAVTSFISPYRKDRDQAREFHK 120
Query: 120 EGALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQQ 179
+ L FIEV+V+ P+EVAEQRDPKGLYK+AR G IKEFTGISAPYE P+SPE+ + + Q
Sbjct: 121 KDGLPFIEVYVECPVEVAEQRDPKGLYKRARAGEIKEFTGISAPYEAPISPEIVVSSHTQ 180
Query: 180 SVEECALKIYEYLVKEKLI 198
S+EEC KI YL+++ LI
Sbjct: 181 SIEECVEKIVNYLLEKDLI 199
>Hs20127475 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine
5'-phosphosulfate kinase
Length = 624
Score = 211 bits (536), Expect = 7e-55
Identities = 102/175 (58%), Positives = 131/175 (74%)
Query: 24 GCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDGDNIRFGLNKDLGFSEQDRNENIR 83
GCT+WLTGLS +GK+T++ ALE+ L+ G+ Y LDGDNIR GLNK+LGFS +DR EN+R
Sbjct: 52 GCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVR 111
Query: 84 RISEVSKLFADSCSIAITSFISPYRIDRDKARELHKEGALKFIEVFVDVPLEVAEQRDPK 143
RI+EV+KLFAD+ + ITSFISPY DR+ AR++H+ +L F EVFVD PL V EQRD K
Sbjct: 112 RIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVK 171
Query: 144 GLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQQSVEECALKIYEYLVKEKLI 198
GLYKKAR G IK FTGI + YE P +PEL L+TD V +C ++ E L + ++
Sbjct: 172 GLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIV 226
>7293733 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine
5'-phosphosulfate kinase
Length = 635
Score = 208 bits (530), Expect = 3e-54
Identities = 109/203 (53%), Positives = 144/203 (70%), Gaps = 5/203 (2%)
Query: 1 MATNITWHQN-LTYDERKE----LRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSA 55
+ATN+T ++ +T + R + R GCT+WLTGLS +GK++IA LE L+++G+ A
Sbjct: 24 VATNVTEQKHHVTRETRGKNLGLCRGFRGCTVWLTGLSGAGKTSIAFELEAYLVSRGIPA 83
Query: 56 YRLDGDNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKAR 115
Y LDGDNIR GLNK+LGF+ DR ENIRR+ EV+KLFADS +AI SF+SP+ DR+ AR
Sbjct: 84 YGLDGDNIRTGLNKNLGFTPADREENIRRVGEVAKLFADSGVVAICSFVSPFADDREMAR 143
Query: 116 ELHKEGALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLR 175
++HK+ LKF E+FVD PL+V E RD KGLYKKAREG+IK FTGI+ YE P PEL +
Sbjct: 144 KIHKDAGLKFYEIFVDTPLDVCETRDVKGLYKKAREGVIKGFTGITQEYERPQMPELVVN 203
Query: 176 TDQQSVEECALKIYEYLVKEKLI 198
T +V E K+ L +E +I
Sbjct: 204 THGYTVRESTQKLVTLLEQEGII 226
>At2g14750 [P] KOG0635 Adenosine 5'-phosphosulfate kinase
Length = 276
Score = 206 bits (525), Expect = 1e-53
Identities = 112/193 (58%), Positives = 132/193 (68%), Gaps = 4/193 (2%)
Query: 2 ATNITWHQ-NLTYDERKELRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDG 60
+TNI WH+ ++ +R+ L Q GC IW+TGLS SGKST+ACAL Q+L KG Y LDG
Sbjct: 78 STNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLCYILDG 137
Query: 61 DNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELHKE 120
DN+R GLN+DL F +DR ENIRR+ EV+KLFAD+ I I S ISPYR DRD R L E
Sbjct: 138 DNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISPYRTDRDACRSLLPE 197
Query: 121 GALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHL-RTDQQ 179
G F+EVF+DVPL V E RDPKGLYK AR G IK FTGI PYE PL+ E+ L R
Sbjct: 198 G--DFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLNCEISLGREGGT 255
Query: 180 SVEECALKIYEYL 192
S E A K+ YL
Sbjct: 256 SPIEMAEKVVGYL 268
>Hs4758880 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine
5'-phosphosulfate kinase
Length = 614
Score = 200 bits (509), Expect = 9e-52
Identities = 94/175 (53%), Positives = 129/175 (73%)
Query: 24 GCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDGDNIRFGLNKDLGFSEQDRNENIR 83
GCT+WLTGLS +GK+TI+ ALE+ L++ + Y LDGDN+R GLN++LGFS DR ENIR
Sbjct: 42 GCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFSPGDREENIR 101
Query: 84 RISEVSKLFADSCSIAITSFISPYRIDRDKARELHKEGALKFIEVFVDVPLEVAEQRDPK 143
RI+EV+KLFAD+ + ITSFISP+ DR+ AR++H+ L F E+FVD PL + E RD K
Sbjct: 102 RIAEVAKLFADAGLVCITSFISPFAKDRENARKIHESAGLPFFEIFVDAPLNICESRDVK 161
Query: 144 GLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQQSVEECALKIYEYLVKEKLI 198
GLYK+AR G IK FTGI + YE P +PE L+T+ +V +C ++ E L ++ ++
Sbjct: 162 GLYKRARAGEIKGFTGIDSDYEKPETPERVLKTNLSTVSDCVHQVVELLQEQNIV 216
>Hs18574866 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine
5'-phosphosulfate kinase
Length = 619
Score = 200 bits (509), Expect = 9e-52
Identities = 94/175 (53%), Positives = 129/175 (73%)
Query: 24 GCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDGDNIRFGLNKDLGFSEQDRNENIR 83
GCT+WLTGLS +GK+TI+ ALE+ L++ + Y LDGDN+R GLN++LGFS DR ENIR
Sbjct: 42 GCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFSPGDREENIR 101
Query: 84 RISEVSKLFADSCSIAITSFISPYRIDRDKARELHKEGALKFIEVFVDVPLEVAEQRDPK 143
RI+EV+KLFAD+ + ITSFISP+ DR+ AR++H+ L F E+FVD PL + E RD K
Sbjct: 102 RIAEVAKLFADAGLVCITSFISPFAKDRENARKIHESAGLPFFEIFVDAPLNICESRDVK 161
Query: 144 GLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQQSVEECALKIYEYLVKEKLI 198
GLYK+AR G IK FTGI + YE P +PE L+T+ +V +C ++ E L ++ ++
Sbjct: 162 GLYKRARAGEIKGFTGIDSDYEKPETPERVLKTNLSTVSDCVHQVVELLQEQNIV 216
>At5g67520 [P] KOG0635 Adenosine 5'-phosphosulfate kinase
Length = 305
Score = 200 bits (508), Expect = 1e-51
Identities = 103/176 (58%), Positives = 124/176 (69%), Gaps = 3/176 (1%)
Query: 4 NITWHQ-NLTYDERKELRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDGDN 62
NI WH +T +R+EL KQ GC IW+TGLS SGKS++ACAL + L N+G +Y LDGDN
Sbjct: 83 NIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYILDGDN 142
Query: 63 IRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELHKEGA 122
+R GLN DL F DR ENIRR+ EV+KLFADS I I S ISPYRI+R R L +G
Sbjct: 143 VRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALLPQG- 201
Query: 123 LKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQ 178
FIEVF+DVPL V E RDPKGLYK+AR G IK FTG+ PYE PL E+ ++ +
Sbjct: 202 -DFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSR 256
>At4g39940 [P] KOG0635 Adenosine 5'-phosphosulfate kinase
Length = 293
Score = 196 bits (499), Expect = 1e-50
Identities = 105/196 (53%), Positives = 130/196 (65%), Gaps = 7/196 (3%)
Query: 2 ATNITWHQN-LTYDERKELRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDG 60
A NI WH++ + +R++L +Q GC +W+TGLS SGKST+ACAL + L +G Y LDG
Sbjct: 92 AENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLDG 151
Query: 61 DNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELHKE 120
DN+R GLN+DL F + R ENIRRI EV+KLFAD I I S ISPYR DRD R L +
Sbjct: 152 DNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYRRDRDACRSLLPD 211
Query: 121 GALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLR----T 176
G F+EVF+DVPL V E RDPKGLYK AR G IK FTGI PYE P++ E+ L+
Sbjct: 212 G--DFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNCEVVLKHTGDD 269
Query: 177 DQQSVEECALKIYEYL 192
+ S + A I YL
Sbjct: 270 ESCSPRQMAENIISYL 285
>At3g03900 [P] KOG0635 Adenosine 5'-phosphosulfate kinase
Length = 208
Score = 195 bits (495), Expect = 4e-50
Identities = 99/175 (56%), Positives = 129/175 (73%), Gaps = 3/175 (1%)
Query: 2 ATNITWHQN-LTYDERKELRKQSGCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRLDG 60
+TNI W ++ + ER++L Q GC +W+TGLS SGKST+AC+L + L N+G +Y LDG
Sbjct: 7 STNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKLSYILDG 66
Query: 61 DNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELHKE 120
DN+R GLNKDLGF +DR ENIRR+ EV+KLFAD+ I I S ISPYR DRD RE+ +
Sbjct: 67 DNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDACREMIQN 126
Query: 121 GALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLR 175
+ FIEVF+++ L++ E RDPKGLYK AR G IK FTGI PYE+PL+ E+ L+
Sbjct: 127 SS--FIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELK 179
>CE06447 [F] KOG4238 Bifunctional ATP sulfurylase/adenosine
5'-phosphosulfate kinase
Length = 652
Score = 189 bits (480), Expect = 2e-48
Identities = 100/200 (50%), Positives = 133/200 (66%), Gaps = 11/200 (5%)
Query: 10 NLTYDERKELRKQS-----------GCTIWLTGLSASGKSTIACALEQLLLNKGLSAYRL 58
N+TY E R++ GCTIW TGLS +GK+TI+ ALE+ L G+ Y L
Sbjct: 31 NITYQEHTISREERAAAVGRHEGFRGCTIWFTGLSGAGKTTISFALERTLNKLGIPCYGL 90
Query: 59 DGDNIRFGLNKDLGFSEQDRNENIRRISEVSKLFADSCSIAITSFISPYRIDRDKARELH 118
DGDNIR GL K+LGFS++DR ENIRR++EV+KLFADS I + +FISP++ DR AR++H
Sbjct: 91 DGDNIRHGLCKNLGFSKEDRQENIRRVAEVAKLFADSGMICLAAFISPFQEDRLDARKIH 150
Query: 119 KEGALKFIEVFVDVPLEVAEQRDPKGLYKKAREGIIKEFTGISAPYETPLSPELHLRTDQ 178
+ +KFIEV V LEV EQRDPK LYKKAR G I FTGI + YE P + E+ L +
Sbjct: 151 ESENVKFIEVHVSTTLEVCEQRDPKQLYKKARAGQILGFTGIDSAYEPPENAEIILDAGK 210
Query: 179 QSVEECALKIYEYLVKEKLI 198
V++C K+ ++L + L+
Sbjct: 211 DGVQQCVQKVLDHLESKGLL 230
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,704,743
Number of Sequences: 60738
Number of extensions: 478598
Number of successful extensions: 1394
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1379
Number of HSP's gapped (non-prelim): 11
length of query: 198
length of database: 30,389,216
effective HSP length: 101
effective length of query: 97
effective length of database: 24,254,678
effective search space: 2352703766
effective search space used: 2352703766
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)