ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII3819 normal G KOG2024 Carbohydrate transport and metabolism Beta-Glucuronidase GUSB (glycosylhydrolase superfamily 2)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII3819 1310501 1313575 1025
(1025 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
7302519 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase s... 105 3e-22
Hs4504223 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase... 85 5e-16
7302094 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase s... 76 3e-13
CE28788 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase s... 68 8e-11
>7302519 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase superfamily
2)
Length = 643
Score = 105 bits (263), Expect = 3e-22
Identities = 111/440 (25%), Positives = 176/440 (39%), Gaps = 78/440 (17%)
Query: 192 SGIYRDVSLLKLPKKAHIEDVRVTTTFVDSQYQDAELSVKVDVQGSSYDHINFTLYEPED 251
+GI+R V L P+ IE+V VTT + E+ V V GS+ + + L +
Sbjct: 177 AGIHRSVHLYTTPR-TFIEEVEVTTN-LSKDATVGEVFYSVSVNGSAANEADNVL---QI 231
Query: 252 GSKVYDASSLLNEENGNTTFSTKEFISFSTKKNEETAFKINVKAPEHWT-----AENPTL 306
+ +YD +L N T +++ K+ V + W +E L
Sbjct: 232 QANLYDKDGILV---ANAT------------SDQKLGGKLQVNPVKPWWPYLMHSEPGYL 276
Query: 307 YKYQLDLIGSDGSVIQSIKHHVGFRQVELKDGNITVNGKDILFRGVNRHDHHPRFGRAVP 366
Y+ ++ L+ ++ ++ + VG R + +NGK + FRG RH+ G+ +
Sbjct: 277 YQLEIKLLATNDELLDVYRLKVGIRTLSWNSQQFLINGKPVYFRGFGRHEDSDIRGKGLD 336
Query: 367 LDFVVRDLILMKKFNINAVRNSHYPNHPKVYDLFDKLGFWVIDEADLETHGVQEPFNRHT 426
+VRD L+K NA R SHYP + D+ G +IDE
Sbjct: 337 NALMVRDFNLLKWIGANAYRTSHYPYSEESMQFADEHGIMIIDECP------------SV 384
Query: 427 NLEAEYPDTKNKLYDVNAHYLSDNPEYEVAYLDRAS--QLVLRDVNHPSIIIWSLGNEAC 484
+ E Y DT + + N E+ ++S QL+ RD NHPS+++WS+ NE
Sbjct: 385 DTELSYSDTSKGFLLMLLASIFRNFSQELLGKHKSSLEQLIHRDRNHPSVVMWSIANEPR 444
Query: 485 YGRNHKAMY-----KLIKQLDPTR------LVHYEGDLNALSADIFSF-----MYPTFEI 528
G Y + LD TR V D S DI SF Y
Sbjct: 445 TGSVSADSYFELVANFTRSLDKTRPITAAIAVSNTQDKAGRSLDIISFNRYNAWYSNAGR 504
Query: 529 MERWRKNHTDE----NGKFEKPLILCEYGHAMGNG------------------PGSLKEY 566
++ +N DE N ++ KP+I+ EYG G K +
Sbjct: 505 LDMITQNVIDEAIAWNKRYNKPIIMSEYGADTLEGLHMQPAYVWSEEFQTEVFSRHFKAF 564
Query: 567 QELFYKEKFYQGGFIWEWAN 586
EL K+ ++ G F+W +A+
Sbjct: 565 DEL-RKKGWFIGEFVWNFAD 583
>Hs4504223 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase superfamily
2)
Length = 651
Score = 85.1 bits (209), Expect = 5e-16
Identities = 111/446 (24%), Positives = 173/446 (37%), Gaps = 117/446 (26%)
Query: 192 SGIYRDVSLLKLPKKAHIEDVRVTTTFVDSQYQDAEL-SVKVDVQGSSYDHINFTLYEPE 250
+G+ R V L P +I+D+ VTT S QD+ L + ++ V+GS+ + L + E
Sbjct: 212 AGLQRSVLLYTTPT-TYIDDITVTT----SVEQDSGLVNYQISVKGSNLFKLEVRLLDAE 266
Query: 251 DGSKVYDASSLLNEENGNTTFSTKEFISFSTKKNEETAFKINVKAPEHWTA----ENPT- 305
N+ N T T ++ V W E P
Sbjct: 267 ------------NKVVANGT---------------GTQGQLKVPGVSLWWPYLMHERPAY 299
Query: 306 LYKYQLDLIG--SDGSVIQSIKHHVGFRQVELKDGNITVNGKDILFRGVNRHDHHPRFGR 363
LY ++ L S G V VG R V + +NGK F GVN+H+ G+
Sbjct: 300 LYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGK 359
Query: 364 AVPLDFVVRDLILMKKFNINAVRNSHYPNHPKVYDLFDKLGFWVIDEADLETHGVQEPFN 423
+V+D L++ NA R SHYP +V + D+ G VIDE + + FN
Sbjct: 360 GFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFN 419
Query: 424 RHTNLEAEYPDTKNKLYDVNAHYLSDNPEYEVAYLDRASQLVLRDVNHPSIIIWSLGNEA 483
+V+ H+ ++ ++V RD NHP++++WS+ NE
Sbjct: 420 -----------------NVSLHH----------HMQVMEEVVRRDKNHPAVVMWSVANEP 452
Query: 484 CYGRNHKAMY-KLI----KQLDPTRLV------HYEGDLNALSADIFSFM--------YP 524
Y K++ K LDP+R V +Y D A D+ Y
Sbjct: 453 ASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWYHDYG 512
Query: 525 TFEIM--------ERWRKNHTDENGKFEKPLILCEYGHAMGNG----PGSL--KEYQELF 570
E++ E W K K++KP+I EYG G P + +EYQ+
Sbjct: 513 HLELIQLQLATQFENWYK-------KYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSL 565
Query: 571 YKE----------KFYQGGFIWEWAN 586
++ K+ G IW +A+
Sbjct: 566 LEQYHLGLDQKRRKYVVGELIWNFAD 591
>7302094 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase superfamily
2)
Length = 686
Score = 75.9 bits (185), Expect = 3e-13
Identities = 79/323 (24%), Positives = 128/323 (39%), Gaps = 72/323 (22%)
Query: 306 LYKYQLDL-IGSDGSVIQSIKHH----VGFRQVELKDGNITVNGKDILFRGVNRHDHHPR 360
LY Q +L + S+ ++S++ VG R + + ++ +NGK + RG RH+
Sbjct: 331 LYNLQFELFVASNEKELESLQDTYRLPVGIRSLSWDNDSLLLNGKPLYLRGFGRHEDSDI 390
Query: 361 FGRAVPLDFVVRDLILMKKFNINAVRNSHYPNHPKVYDLFDKLGFWVIDEADLETHGVQE 420
G+ + + RD L+K NA R SHYP + D+ G +IDE + E
Sbjct: 391 RGKGLDNALLARDFNLLKWTGANAYRTSHYPYSEESMQFADQHGIMIIDECPAVNIDIFE 450
Query: 421 PFNRHTNLEAEYPDTKNKLYDVNAHYLSDNPEYEVAYLDRASQLVLRDVNHPSIIIWSLG 480
P L +N ++ QL+ RD NHPS++ WS+
Sbjct: 451 P------------------------QLLEN------HMSSLEQLIHRDRNHPSVVAWSVA 480
Query: 481 NEACYGRNHKAMY-----KLIKQLDPTRLVHYEGDLNALSADIFSFM-YPTFEIMERWRK 534
NE + Y ++++ R + + N+ S + F+ F W +
Sbjct: 481 NEPRSNKQGALKYFEFLVNYVREIAHGRPLTAAINANSSSCHLAQFLDIVGFNRYNSWYQ 540
Query: 535 N--HTDE------------NGKFEKPLILCEYGHAMGNGPGSL------KEYQ-ELFYK- 572
N TD +F KP+I EYG G SL +EYQ ELF +
Sbjct: 541 NSGRTDMILNLLTIEAQSWRDRFGKPVIQFEYGGDTMEGMHSLPAFIWSEEYQVELFSRH 600
Query: 573 ---------EKFYQGGFIWEWAN 586
++ G F+W +A+
Sbjct: 601 FKAFDELRGRGWFIGEFVWNFAD 623
>CE28788 [G] KOG2024 Beta-Glucuronidase GUSB (glycosylhydrolase superfamily
2)
Length = 628
Score = 67.8 bits (164), Expect = 8e-11
Identities = 94/397 (23%), Positives = 144/397 (35%), Gaps = 102/397 (25%)
Query: 122 SFEHRLRFEGVDNCYELYVNGQYVGFNKGSRNGAEFDIQKYVSEG-ENLVVVKV---FKW 177
+ H LRF V+ +Y+N + V + G E DI + G EN V V W
Sbjct: 108 NMRHVLRFGSVNYFAVVYINSEKVTSHIGGHLPFEVDISAQIKFGAENKFTVAVNNTLSW 167
Query: 178 S-----DSTY-----------------------IEDQDQWWLSGIYRDVSLLKLPKKAHI 209
S D Y + + D + +GI R V L+K+P +I
Sbjct: 168 STIPQGDFNYQSVAPRNISGRILSRLPAGAVKNVGNFDFFNYAGILRSVQLMKIP-SVYI 226
Query: 210 EDVRVTTTFVDSQYQDAELSVKVDVQGSSYDHINFTLYEPEDGSKVYDASSLLNEENGNT 269
+++ + S + + +S V+ + +++ E GS VY GN
Sbjct: 227 QNINIVADHTGSFFFETAVSSLDGVR------VEVKMFDGE-GSLVY---------TGNQ 270
Query: 270 TFSTKEFISFSTKKNEETAFKINVKAPEHWTAE---NPTLYKYQLDLIGSDGSVIQSIKH 326
T S + + P+ W P LY ++ LI DG + +
Sbjct: 271 TKSEGQ-----------------ISNPKLWWPRGMGKPDLYSLEVSLI-LDGELADIYRE 312
Query: 327 HVGFRQVELKDGNITVNGKDILFRGVNRHDHHPRFGRAVPLDFVVRDLILMKKFNINAVR 386
GFR V D I +N K G H+ GR + +DL L++ N R
Sbjct: 313 QFGFRTVTWSDSQIFINSKPFYCLGFGMHEDFEIIGRGFNQAIMTKDLNLLEWMGGNCYR 372
Query: 387 NSHYPNHPKVYDLFDKLGFWVIDEADLETHGV-QEPFNRHTNLEAEYPDTKNKLYDVNAH 445
+HYP + D+ G VI +ET V + F++ N N H
Sbjct: 373 TTHYPYSEERMFENDRRGIAVI----VETPAVGLKGFSKANN---------------NLH 413
Query: 446 YLSDNPEYEVAYLDRASQLVLRDVNHPSIIIWSLGNE 482
+ ++ RD NHPS+I WSL NE
Sbjct: 414 ------------VKMLQDMIDRDKNHPSVIAWSLANE 438
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.137 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,544,943
Number of Sequences: 60738
Number of extensions: 3266735
Number of successful extensions: 7117
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 7106
Number of HSP's gapped (non-prelim): 8
length of query: 1025
length of database: 30,389,216
effective HSP length: 116
effective length of query: 909
effective length of database: 23,343,608
effective search space: 21219339672
effective search space used: 21219339672
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)