ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII0119  suspect: LH   U     KOG0946  Intracellular trafficking, secretion, and vesicular transport  ER-Golgi vesicle-tethering protein p115

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII0119  38174 35400 -925 
         (925 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115            54   1e-06

>YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115
          Length = 1790

 Score = 53.5 bits (127), Expect = 1e-06
 Identities = 98/428 (22%), Positives = 177/428 (40%), Gaps = 66/428 (15%)

Query: 13   KKKGKTYSKANDAEDEELNVGEHKFLAENLLERVKKRLNGDSDDSTASAIDNSKKSTFFY 72
            K KG+  S   + E        H+ L E L+    +    D      ++  +S K  F  
Sbjct: 741  KLKGEITSLQTETEST------HENLTEKLIALTNEHKELDEKYQILNSSHSSLKENFSI 794

Query: 73   SDTDLYEGKELDDEFEAMRETAAVPKQREVEIQQKKHPISQTQLIPTFSSSWLAKTQVNT 132
             +T+L   ++  DE   +R           ++ + K   +QT L+     S + K + + 
Sbjct: 795  LETELKNVRDSLDEMTQLR-----------DVLETKDKENQTALLE--YKSTIHKQEDSI 841

Query: 133  QALEGTNRTIDRQQQDLDTQLN---------NEEITQTTPDEHTLDKAATESKLLHSKET 183
            + LE    TI  Q++  +  +N         + E+     +   L K   +S + H KET
Sbjct: 842  KTLEKGLETILSQKKKAEDGINKMGKDLFALSREMQAVEENCKNLQKEKDKSNVNHQKET 901

Query: 184  SFRNTSFTFKLTTNN--NEGLLATKPNGTQIENEQKVVATQLDVAEKEKAVP---LIKDT 238
                     K+T     NE L   K     +  E++ ++ +L V  K +      L+   
Sbjct: 902  KSLKEDIAAKITEIKAINENLEEMKIQCNNLSKEKEHISKEL-VEYKSRFQSHDNLVAKL 960

Query: 239  IEEPHLPVAPLKAVRKKYLP-----EQFIAAFDDSESSNAEDLSDEENDADELKDMASTQ 293
             E+       LK++   Y       E  I A ++S++ ++  LS+ +N  D +      +
Sbjct: 961  TEK-------LKSLANNYKDMQAENESLIKAVEESKNESSIQLSNLQNKIDSMSQ--EKE 1011

Query: 294  ELVTKQKSVVTNMNGIIAITMKNSPQNVIELNSSSE-SEDDYKSQSSKAALLLIKAKRSR 352
                ++ S+  N+   +  T+ +  Q   E+ S S+ S+D+Y+SQ S     L+K K   
Sbjct: 1012 NFQIERGSIEKNIEQ-LKKTISDLEQTKEEIISKSDSSKDEYESQIS-----LLKEK--L 1063

Query: 353  NKATKAKNMHVSTVDSLKELFTSLKT-----KNRDQILEFRREISGK--KGI--SLEAIE 403
              AT A + +V+ +  L +    L+      KN    LE + E S K  K +  + E ++
Sbjct: 1064 ETATTANDENVNKISELTKTREELEAELAAYKNLKNELETKLETSEKALKEVKENEEHLK 1123

Query: 404  DEKIQVEK 411
            +EKIQ+EK
Sbjct: 1124 EEKIQLEK 1131


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.307    0.125    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,376,365
Number of Sequences: 60738
Number of extensions: 1973741
Number of successful extensions: 8691
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 8688
Number of HSP's gapped (non-prelim): 5
length of query: 925
length of database: 30,389,216
effective HSP length: 116
effective length of query: 809
effective length of database: 23,343,608
effective search space: 18884978872
effective search space used: 18884978872
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)