ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0213 suspect: LH S KOG1894 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII0213  68760 67339 -474 
         (474 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR527w [S] KOG1894 Uncharacterized conserved protein                 422   e-118
SPBC947.13 [S] KOG1894 Uncharacterized conserved protein              132   1e-30

>YDR527w [S] KOG1894 Uncharacterized conserved protein
          Length = 439

 Score =  422 bits (1085), Expect = e-118
 Identities = 231/479 (48%), Positives = 313/479 (65%), Gaps = 53/479 (11%)

Query: 1   MDLFGDIVERETVAPDAVSNQGMPT-------TGFPKLHQPEKVSSWKQRLMEXXXXXXX 53
           MDL GDIVE++T   D+V +    T       TGFP+L++P+K+SSWK+RL E       
Sbjct: 1   MDLLGDIVEKDT--SDSVESNDNGTLSTNNCGTGFPELYKPKKISSWKERLREKRAQKKK 58

Query: 54  RSNTGSEPPNANFVEVGNDSCFSDDTKTDPNALSEAERIHLQNLAVLQSMTPEQFERERQ 113
            S   +E                  T TD   LSEA+ IH +N+ VLQ M+ EQ  +ER+
Sbjct: 59  TSGKDAE---------------KQQTSTDA-PLSEAKSIHNENIKVLQGMSDEQIVQERE 102

Query: 114 ELMDSLNPNVLKSLIARVDKKMTSLSLKDGETSRSKPPVPQYAEIEGP-GTWIGGSNK-V 171
           +L +SL+P ++  L+  ++K+           ++ +   P +AEIEG  GTW+GG+ + +
Sbjct: 103 DLYNSLDPKLIAKLLKNINKR-----------AKDENNTPLFAEIEGASGTWVGGNKQGI 151

Query: 172 KDLPKLDDKAVDEALGINSVEVQPLKSVRFERDLKKEEVHNIKPDEYDRTDEVPNGTFND 231
            DLP LDD+ VD AL I  +  +  K V+FE   K+++V            E    T +D
Sbjct: 152 YDLPPLDDEDVDVALEIRPMLGKDAKHVQFEEAGKEKDV------------EGEGKTNDD 199

Query: 232 EDDMAPQEYQFVQSIDHMSNKDLLAEVHFIKARKTMGKEQEYEPLDINDPNFDQELHKKF 291
            DD+AP ++Q  Q IDHM N++L  +VHFIK       E   E LDINDPNF+ +LH+K+
Sbjct: 200 VDDIAPLDFQMAQCIDHMKNEELFKDVHFIKEESQ--NEINLEKLDINDPNFNDKLHEKY 257

Query: 292 FPDLPQEPDKLKWMQQVENTGKAEEVIHDVSVCRFDFKGNMVPPGRKLTTT-NNGLHHHS 350
           FPDLP+E DKLKWMQ V+       +I DVS CRFDF G++VPP R++ +T ++GLHHHS
Sbjct: 258 FPDLPKEVDKLKWMQPVQQKTDKNYIIEDVSECRFDFNGDLVPPTRQIDSTIHSGLHHHS 317

Query: 351 DAPELAGYTIPELAHLSLSKFPAQRSIAIQTLGRILYKLGKQSYAQLVPEVDAETYQEEG 410
           D+PELAGYTI EL HL+ S FP+QR IAIQTLGRILYKLG++SY QLVPE+DA+TY+E+G
Sbjct: 318 DSPELAGYTIVELEHLARSTFPSQRCIAIQTLGRILYKLGQKSYYQLVPEIDADTYKEDG 377

Query: 411 GSKQIINKIYYMFWDVMKDCKVIETLQLAADESKTANISVRNYAIDALWLWKQGGGDLR 469
               +++KIY MFWD++KD KVIE+L++++DE  T N+SVRNYAIDALWLWKQGGGD R
Sbjct: 378 SISNVMDKIYSMFWDLIKDGKVIESLEISSDEKFTRNLSVRNYAIDALWLWKQGGGDFR 436


>SPBC947.13 [S] KOG1894 Uncharacterized conserved protein
          Length = 452

 Score =  132 bits (332), Expect = 1e-30
 Identities = 111/392 (28%), Positives = 182/392 (46%), Gaps = 55/392 (14%)

Query: 95  QNLAVLQSMTPEQFERERQELMDSLNPNVLKSLIARVDKKMTSLSLKDGETSRSKPPVPQ 154
           +N   L+ M   + E  ++E+  ++  ++L+ L  R  KK     L   +   S+   P 
Sbjct: 83  ENRVRLERMNDLEIEGAQEEIRATIRDDLLEMLKKRAFKKKAERELAQRKDRSSQVNTPD 142

Query: 155 YAEIEGPGTWIGGSNKVKDLPKLD--------DKAVDEALGINSVEVQPLKSVRFERDLK 206
            ++     +++  + K++   KL+         +AVD + G  S  +  L          
Sbjct: 143 LSQRPSDDSFLS-NEKLRSSEKLNRNLQSVLSSEAVDSSSGSPSPPMA-LSQAEIRSRQT 200

Query: 207 KEEVHNIKPDEYDRTDEVPNGT---FNDEDDMA------PQEYQFVQSIDHMSNKDLLAE 257
           K  +   K +E  +   +P       N+EDD +      P+++    S    SN     E
Sbjct: 201 KRVMFPDKAEELTKIFSLPTLAPIKGNEEDDASEDAKHSPKKHSPALSDGTTSNDGAPLE 260

Query: 258 VHFIKARKTMGKEQEYEPLDINDPNFDQELHKKFFPDLPQEPDKLKWMQQ---VENT-GK 313
                   T    ++   LD NDP+F ++LH K+FP+LP +  +++W+      EN+   
Sbjct: 261 FD------TTHLPEKQVTLDPNDPSFYEQLHDKYFPNLPVDEKQMQWLHDPSPAENSYHP 314

Query: 314 AEEVIHDVSVCRFDFKGNMVPPGRKLTT-TNNGLHHHSDAPELAGYTIPELAHLSLSKFP 372
           + E +H   + RF FKG ++ P +  T   N GLHHH DAP  AGYT+ ELAHL  S FP
Sbjct: 315 SVESLHAHEI-RFGFKGEIITPSQSQTIPVNEGLHHHGDAPFSAGYTLVELAHLLRSSFP 373

Query: 373 AQRSIAIQTLGRILYKLGKQSYAQ-LVPEVDAETYQEEGGSKQIINKIYYMFWDVMKDCK 431
            QR IAIQT+GRI+Y+L    + + L PE+                        +++D  
Sbjct: 374 TQRCIAIQTIGRIIYRLNSGEFREVLSPELHT----------------------LVEDAH 411

Query: 432 VIETLQLAADESKTANISVRNYAIDALWLWKQ 463
           + E L  AA + +  +++VR+ AI+ALWL  Q
Sbjct: 412 IYELLAAAASD-QVKHLTVRSLAIEALWLCSQ 442


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.132    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,054,415
Number of Sequences: 60738
Number of extensions: 1403600
Number of successful extensions: 3110
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3100
Number of HSP's gapped (non-prelim): 2
length of query: 474
length of database: 30,389,216
effective HSP length: 110
effective length of query: 364
effective length of database: 23,708,036
effective search space: 8629725104
effective search space used: 8629725104
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)