ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0265 good U KOG3834 Intracellular trafficking, secretion, and vesicular transport Golgi reassembly stacking protein GRASP65, contains PDZ domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0265 87029 85866 -388
(388 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR517w [U] KOG3834 Golgi reassembly stacking protein GRASP65 co... 337 1e-92
SPAC1D4.02c [U] KOG3834 Golgi reassembly stacking protein GRASP6... 89 8e-18
CE25278 [U] KOG3834 Golgi reassembly stacking protein GRASP65 co... 68 3e-11
7293723 [U] KOG3834 Golgi reassembly stacking protein GRASP65 co... 62 2e-09
Hs20127539 [U] KOG3834 Golgi reassembly stacking protein GRASP65... 60 7e-09
Hs13994253 [U] KOG3834 Golgi reassembly stacking protein GRASP65... 58 2e-08
ECU01g0530 [U] KOG3834 Golgi reassembly stacking protein GRASP65... 53 7e-07
>YDR517w [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 372
Score = 337 bits (865), Expect = 1e-92
Identities = 180/376 (47%), Positives = 244/376 (64%), Gaps = 42/376 (11%)
Query: 1 MFRIAKNLVRTLEESVTGTVG----DKPIDSYFHSVPPQLFPIREDDP--------RYAH 48
MFRIAKNLVRT E+SV T+ +D++F S+PP L + + P ++ +
Sbjct: 1 MFRIAKNLVRTFEQSVQDTLALSQDSSNLDAFFQSIPPNLLSAQLESPVDAVSEGVKHTN 60
Query: 49 QTDSLYGLRVVQVDESQLQLTSFFDYIIGFNNQPIPLVQDGTMGASQTFYPDYKRIISFF 108
++L GLR+V VDE Q QL SFFDYI+GFN+ P+P+V + G S YPDY+RI S F
Sbjct: 61 VNETLSGLRIVWVDEMQFQLQSFFDYIVGFNDDPVPVVSN-QHGFS---YPDYRRITSIF 116
Query: 109 NEHCGNTIKLHVWSGKGGVYREEYLYVSAKNS--VSEIPLDETDAQQSLMTSKMFEALGF 166
NEHCG T+K+++WS KGG +R+EY+ + +K S + ++ L+ + + S + F+ALGF
Sbjct: 117 NEHCGRTLKVNIWSAKGGTFRDEYISIISKESDDLDDVSLNHDERRPSSGEAHQFQALGF 176
Query: 167 KVQWTPLIAVTYSYHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLLQDIVRS 226
KVQWTPLIA T++YH+L +NI +GPA AGLIPD+DYII CQDGLLATGGE LLQDIVRS
Sbjct: 177 KVQWTPLIASTFTYHILNVNIPDGPAQSAGLIPDEDYIIGCQDGLLATGGETLLQDIVRS 236
Query: 227 RANHSLVLYVYNKAVDCVRPVTVHLAADGRLGCNVGYGFLHRIPAPIQNSQSIIHSPAAV 286
RAN+ LVLYVYNK DCVRP+TVH+ DGRLGCNVGYGFLHRIP ++ H P
Sbjct: 237 RANYDLVLYVYNKVSDCVRPITVHIGPDGRLGCNVGYGFLHRIP-------TVKHCPQQA 289
Query: 287 ESHNSNNIIHPITQPTLIPATLANNADNKETMPSNPLQVPVPVGRRQAQLHRGXXXXXXX 346
+ ++ +P+ P + + A +PS PVP +++++ +G
Sbjct: 290 QQQGQDD--NPVPVPVPVESETA-------FVPSAFTAPPVPT-KKKSKNKKG------- 332
Query: 347 XXXXXMADYFQEGKDQ 362
M DYF EG+D+
Sbjct: 333 TQPLAMDDYFNEGRDK 348
>SPAC1D4.02c [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 345
Score = 89.4 bits (220), Expect = 8e-18
Identities = 58/191 (30%), Positives = 96/191 (49%), Gaps = 23/191 (12%)
Query: 162 EALGFKVQWTPLI-AVTYSYHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLL 220
E +G +QW + AV +H+L + I++ P +A L+P +DYI+ +G++ GE L
Sbjct: 101 EKIGMVLQWASIAPAVDAIWHILNV-IDDSPVARASLVPYEDYIVGTPEGMMT--GEKAL 157
Query: 221 QDIVRSRANHSLVLYVYNKAVDCVRPVTV----HLAADGRLGCNVGYGFLHRIPAPIQNS 276
D++ S N L LY+YN D R VT+ H +G +GC VG+G LHR+PAP+
Sbjct: 158 SDLIESHLNRPLRLYIYNHYRDSTRQVTIVPNRHWGGNGAIGCGVGHGVLHRLPAPLSGP 217
Query: 277 QSIIHSPAAVESHNSNNII----HPITQPT----LIPATLANNADNKETMPSNPLQVPVP 328
P + SN ++ H ++QP+ +P + SN ++ +P
Sbjct: 218 -----PPQPGDIVFSNPMLGGPDHKVSQPSETENFLPTPEPPKIASANAGSSN--EISIP 270
Query: 329 VGRRQAQLHRG 339
+R + H+G
Sbjct: 271 HYQRHKKSHKG 281
>CE25278 [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 506
Score = 67.8 bits (164), Expect = 3e-11
Identities = 64/236 (27%), Positives = 101/236 (42%), Gaps = 50/236 (21%)
Query: 55 GLRVVQVDESQ----LQLTSFFDYIIGFNNQPIPLVQDGTMGASQTFYPDYKRIISFFNE 110
G V++V E+ L FFD+I+ N + D TM K ++ +
Sbjct: 16 GYHVLRVQENSPGAVAGLEPFFDFIVSIGNIRLDKDND-TM----------KEVLK---Q 61
Query: 111 HCGNTIKLHVWSGKGGVYREEYLYVSAKNSVSEIPLDETDAQQSLMTSKMFEALGFKVQW 170
H +++ V++ K R+ S +P Q L S F + Q
Sbjct: 62 HIEKPLEITVYNSKSQAVRQ----------TSIVPSQNWGGQGLLGVSIRFCSFDGASQ- 110
Query: 171 TPLIAVTYSYHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLLQDI---VRSR 227
+ +H++ + N PA AGLI D DYI+ + L H D+ V++
Sbjct: 111 -------HVWHIISVQ-PNSPASLAGLIADTDYILGAESVL------HQADDLIALVQAN 156
Query: 228 ANHSLVLYVYNKAVDCVRPVTVH----LAADGRLGCNVGYGFLHRIPAPIQNSQSI 279
L LYVYN D VR V++ +G LGC++GYG+LHRIP + S+++
Sbjct: 157 EGKPLKLYVYNVDTDVVREVSLTPNSAWGGEGCLGCDIGYGYLHRIPVSVDRSKAV 212
>7293723 [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 460
Score = 61.6 bits (148), Expect = 2e-09
Identities = 39/116 (33%), Positives = 61/116 (51%), Gaps = 11/116 (9%)
Query: 180 YHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLLQDIVRSRANHSLVLYVYNK 239
+H+L+++ N PA AGL DY+I D + + L ++ + L +YVYN
Sbjct: 113 WHILEVH-PNSPAELAGLRAYSDYVIGA-DAIRHENDD--LFTLIETHEQQPLKMYVYNL 168
Query: 240 AVDCVRPVTVH----LAADGRLGCNVGYGFLHRIPA---PIQNSQSIIHSPAAVES 288
D R VT+ +G LGC +G+G+LHRIP P+ +S + + + AA ES
Sbjct: 169 DDDACREVTIKPNTAWGGEGALGCGIGFGYLHRIPVQAEPVTSSSAAVPAAAAAES 224
>Hs20127539 [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 490
Score = 59.7 bits (143), Expect = 7e-09
Identities = 39/105 (37%), Positives = 52/105 (49%), Gaps = 9/105 (8%)
Query: 180 YHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLLQDIVRSRANHSLVLYVYNK 239
+HVL++ N PA AGL P DYII + E L ++ + L LYVYN
Sbjct: 113 WHVLEVE-SNSPAALAGLRPHSDYIIGADT--VMNESEDLFS-LIETHEAKPLKLYVYNT 168
Query: 240 AVDCVRPVTVH----LAADGRLGCNVGYGFLHRIPA-PIQNSQSI 279
D R V + +G LGC +GYG+LHRIP P + + I
Sbjct: 169 DTDNCREVIITPNSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKI 213
>Hs13994253 [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 440
Score = 58.2 bits (139), Expect = 2e-08
Identities = 38/101 (37%), Positives = 51/101 (49%), Gaps = 20/101 (19%)
Query: 180 YHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLLQD------IVRSRANHSLV 233
+HVL + + PA AGL P DY++ G + +LQ+ ++ S L
Sbjct: 113 WHVLDVE-PSSPAALAGLRPYTDYVV---------GSDQILQESEDFFTLIESHEGKPLK 162
Query: 234 LYVYNKAVDCVRPVTVHLAA----DGRLGCNVGYGFLHRIP 270
L VYN D R VTV A +G LGC +GYG+LHRIP
Sbjct: 163 LMVYNSKSDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIP 203
>ECU01g0530 [U] KOG3834 Golgi reassembly stacking protein GRASP65 contains PDZ
domain
Length = 545
Score = 53.1 bits (126), Expect = 7e-07
Identities = 36/107 (33%), Positives = 62/107 (57%), Gaps = 4/107 (3%)
Query: 164 LGFKVQWTPLIAVTYSYHVLQLNIENGPAMQAGLIPDQDYIIACQDGLLATGGEHLLQDI 223
LG V++ IA S VL++N E+ PA +AG++ DYI+ ++ + + E LL+ +
Sbjct: 85 LGISVKFHQSIACLLSMEVLRVNPES-PAEKAGMVVG-DYILGIEN-IYSKDEEDLLRFL 141
Query: 224 VRSRANHSLVLYVYNKAVDCVRPVTVHLAADGRLGCNVGYGFLHRIP 270
+R + L VYN ++ VR V++ + D LGC +G G L+++P
Sbjct: 142 EMNR-RRVVPLLVYNSDLEYVRAVSLQVGVDVLLGCQIGMGELYKVP 187
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,949,506
Number of Sequences: 60738
Number of extensions: 962101
Number of successful extensions: 2124
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2106
Number of HSP's gapped (non-prelim): 9
length of query: 388
length of database: 30,389,216
effective HSP length: 108
effective length of query: 280
effective length of database: 23,829,512
effective search space: 6672263360
effective search space used: 6672263360
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)