ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII0312  suspect: LH   U     KOG1087  Intracellular trafficking, secretion, and vesicular transport  Cytosolic sorting protein GGA2/TOM1

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII0312  103218  104222 335  
         (335 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC31F10.07 [U] KOG1087 Cytosolic sorting protein GGA2/TOM1           97   3e-20

>SPBC31F10.07 [U] KOG1087 Cytosolic sorting protein GGA2/TOM1
          Length = 304

 Score = 97.1 bits (240), Expect = 3e-20
 Identities = 92/341 (26%), Positives = 160/341 (45%), Gaps = 44/341 (12%)

Query: 1   MGFFTDH-PYTSVTESINKAVISENATLEVELGNILQLIRTGD-TDTNQVEAARAIRKRL 58
           MG F++  P T+VT  I++  ++   T + +L  I+QL    + T T   EA+R +RK+L
Sbjct: 1   MGIFSETVPITAVTTYIDR--LTSRDTDDEDLSGIVQLSEAVNLTVTGPREASRTLRKKL 58

Query: 59  KHGDLYQQSRALDLLNLFVSQL-IHLPLFYSDFRLINVLLDMSSNSGKYPKQISRKCTCY 117
           K+   ++Q RAL +L   +     H    +SD +L + +L  ++NS +Y K + ++    
Sbjct: 59  KYSTPHEQVRALVILQALIENAGSHFLQNFSDEKLEDRMLQCATNS-EYSKPVRKRAIHM 117

Query: 118 LIGWYQYLASHQEVEGFEMLFTVCEKAYKNKKRHTKVMNKSYQKKKKALPQFLSDTADRR 177
           +  W+     +  V G E + ++  +                      LPQ  S  +   
Sbjct: 118 IKLWHN---DYSNVRGMESMSSLVSR----------------------LPQRQSSASHSE 152

Query: 178 AVTADERYGIPRIDLEKEAPKIKLLISDSLETAVSLSNALLAI-PAHSKSTDSELCTAKF 236
                     P I+L+K  P ++ LI+ S   A +LSN+L+ I P       ++     +
Sbjct: 153 Q---------PTINLKKVGPILERLIASSSMAATNLSNSLVRINPNTENPAKNKQIMVYY 203

Query: 237 VQARALRRKVLRYLQLITGGELLGALLHANEELVNALMAF-DQLAEEGDMASLNERDSDE 295
           V  +   R +LRY+Q I     L  LL AN+E+V A+ AF ++ +E  D +S +   S  
Sbjct: 204 VDCKRAHRSLLRYIQAIQDEMWLANLLKANDEIVTAIDAFKEKCSENSDYSSDSGSYSSS 263

Query: 296 DDQ--EDNEAYISDEASIPSYTSRGGAAQPNDPFSDNNKIK 334
             +  +D  +YIS  +S  S   R      N+PF D+N+++
Sbjct: 264 YSRHLDDRASYISRSSSGGSNAQRSEDLDVNNPFGDHNRLE 304


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.132    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,108,542
Number of Sequences: 60738
Number of extensions: 793714
Number of successful extensions: 4596
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4594
Number of HSP's gapped (non-prelim): 2
length of query: 335
length of database: 30,389,216
effective HSP length: 107
effective length of query: 228
effective length of database: 23,890,250
effective search space: 5446977000
effective search space used: 5446977000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)