ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0466 good J KOG3073 Translation, ribosomal structure and biogenesis Protein required for 18S rRNA maturation and 40S ribosome biogenesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0466 152834 153589 252
(252 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR186w [J] KOG3073 Protein required for 18S rRNA maturation and... 480 e-136
SPAC18G6.07c [J] KOG3073 Protein required for 18S rRNA maturatio... 301 4e-82
CE19133 [J] KOG3073 Protein required for 18S rRNA maturation and... 202 3e-52
At3g57000 [J] KOG3073 Protein required for 18S rRNA maturation a... 194 8e-50
7290503 [J] KOG3073 Protein required for 18S rRNA maturation and... 186 2e-47
Hs15625590 [J] KOG3073 Protein required for 18S rRNA maturation ... 160 1e-39
ECU01g0480 [J] KOG3073 Protein required for 18S rRNA maturation ... 77 3e-14
>YLR186w [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 252
Score = 480 bits (1235), Expect = e-136
Identities = 232/252 (92%), Positives = 247/252 (97%)
Query: 1 MVEDSKVRDAMKNGEKKVLPASLVPQAPPVLTSKDKVTKRLIVVLSQASLETHKISSNGP 60
MVEDS+VRDA+K G++K LPASLVPQAPPVLTSKDK+TKR+IVVL+ ASLETHKISSNGP
Sbjct: 1 MVEDSRVRDALKGGDQKALPASLVPQAPPVLTSKDKITKRMIVVLAMASLETHKISSNGP 60
Query: 61 GGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPVNKAGKLQVYIQTSRGV 120
GGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSP+NKAGKLQVYIQTSRG+
Sbjct: 61 GGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTSRGI 120
Query: 121 LIELNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKITL 180
LIE+NPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRK+TL
Sbjct: 121 LIEVNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTL 180
Query: 181 SFDAPVIRVQDYVEKLDDDESICVFVGAMARGKDNFADEFVDEKIGLSNYPLSASVACSK 240
SFDAPVIRVQDY+EKLDDDESICVFVGAMARGKDNFADE+VDEK+GLSNYPLSASVACSK
Sbjct: 181 SFDAPVIRVQDYIEKLDDDESICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSK 240
Query: 241 FCHGCEDVWNIM 252
FCHG ED WNI+
Sbjct: 241 FCHGAEDAWNIL 252
>SPAC18G6.07c [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 359
Score = 301 bits (772), Expect = 4e-82
Identities = 150/221 (67%), Positives = 181/221 (81%), Gaps = 4/221 (1%)
Query: 33 SKDKVTKRLIVVLSQASLETHKISSNGPGGD-KYVLLNCDDHQGLLKKMGRDISEARPDI 91
S D T+RLIVVL QA LE +K+ G D KY LLNCDDHQG+LKK+ R+I++ARPDI
Sbjct: 142 SHDTTTQRLIVVLDQACLEIYKV---GKAKDAKYQLLNCDDHQGILKKLNRNIAQARPDI 198
Query: 92 THQCLLTLLDSPVNKAGKLQVYIQTSRGVLIELNPTVRIPRTFKRFSGLMVQLLHKLSIR 151
THQCLLTLLDSP+NKAG+LQVYI T++ VLIE+NP+VRIPRTFKRFSGLMVQLLHKLSIR
Sbjct: 199 THQCLLTLLDSPLNKAGRLQVYIHTAKKVLIEVNPSVRIPRTFKRFSGLMVQLLHKLSIR 258
Query: 152 SVNSEEKLLKVIKNPITDHLPTKCRKITLSFDAPVIRVQDYVEKLDDDESICVFVGAMAR 211
SVN EKLLKVIKNP+TD+LP CRK TLSFDAP + + Y+E L ++S+C+ +GAMA
Sbjct: 259 SVNGNEKLLKVIKNPVTDYLPPNCRKATLSFDAPTVPPRKYLETLQPNQSVCIAIGAMAH 318
Query: 212 GKDNFADEFVDEKIGLSNYPLSASVACSKFCHGCEDVWNIM 252
G D+F+D +VDEKI +S+YPLSAS+ACSKF H ED I+
Sbjct: 319 GPDDFSDGWVDEKISISDYPLSASIACSKFLHSMEDFLGIV 359
>CE19133 [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 231
Score = 202 bits (515), Expect = 3e-52
Identities = 101/229 (44%), Positives = 153/229 (66%), Gaps = 9/229 (3%)
Query: 25 PQAPPVLTSKDKVTKRLI-VVLSQASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGRD 83
P A + T K+++ VVL SLET K+ G +Y +L+ D H L+K +D
Sbjct: 11 PNAKRMKTDNQLEDKKILYVVLEGCSLETAKV------GGEYAILSSDKHANFLRKQKKD 64
Query: 84 ISEARPDITHQCLLTLLDSPVNKAGKLQVYIQTSRGVLIELNPTVRIPRTFKRFSGLMVQ 143
++ RPDI HQCLL LLDSP+N+AGKL+V+ +TS+ VL++++P RIPRTF RF GLMVQ
Sbjct: 65 PADYRPDILHQCLLNLLDSPLNRAGKLRVFFRTSKNVLVDVSPQCRIPRTFDRFCGLMVQ 124
Query: 144 LLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKITLSFDAPVIRVQDYVEKLDDDESIC 203
LLHKLSIR+ + +KL+ V+KNP+++HLP RK+ +SF+ P + + + + + DE +
Sbjct: 125 LLHKLSIRAAETTQKLMSVVKNPVSNHLPVGSRKMLMSFNVPELTMANKLVAPETDEPLV 184
Query: 204 VFVGAMARGKDNFADEFVDEKIGLSNYPLSASVACSKFCHGCEDVWNIM 252
+ +G +ARGK ++ D + +SNYPLSA++ C+K G E++W I+
Sbjct: 185 LIIGGIARGK--IVVDYNDSETKISNYPLSAALTCAKVTSGLEEIWGII 231
>At3g57000 [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 298
Score = 194 bits (494), Expect = 8e-50
Identities = 111/234 (47%), Positives = 152/234 (64%), Gaps = 12/234 (5%)
Query: 23 LVPQAPPVLTSKDKVTKRLIVVLSQASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGR 82
+V P VL + +K ++ VL +ASLE K+ G Y LLN DDH LKK R
Sbjct: 73 IVGGIPIVLNAPNKEKSGIVFVLEKASLEVAKV------GKTYQLLNSDDHANFLKKNNR 126
Query: 83 DISEARPDITHQCLLTLLDSPVNKAGKLQ-VYIQTSRGVLIELNPTVRIPRTFKRFSGLM 141
+ ++ RPDITHQ LL +LDSPVNKAG+L+ VY++T +GVL E+ P VRIPRTFKRF+G+M
Sbjct: 127 NPADYRPDITHQALLMILDSPVNKAGRLKAVYVRTEKGVLFEVKPHVRIPRTFKRFAGIM 186
Query: 142 VQLLHKLSIRSVNSEEKLLKVIKNPITD-HLPTKCRKITLSFDA-PVIRVQDYVEKLDDD 199
+QLL KLSI +VNS EKLL+ +KNPI + HLP +I S + ++ +Q ++ + DD
Sbjct: 187 LQLLQKLSITAVNSREKLLRCVKNPIEEHHLPVNSHRIGFSHSSEKLVNMQKHLATVCDD 246
Query: 200 ESICVF-VGAMARGKDNFADEFVDEKIGLSNYPLSASVACSKFCHGCEDVWNIM 252
+ VF VGAMA GK + ++DE + +S YPLSA+ S+ C WNI+
Sbjct: 247 DRDTVFVVGAMAHGKIDC--NYIDEFVSVSEYPLSAAYCISRICEALATNWNII 298
>7290503 [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 233
Score = 186 bits (473), Expect = 2e-47
Identities = 101/220 (45%), Positives = 134/220 (60%), Gaps = 33/220 (15%)
Query: 39 KRLIVVLSQASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLT 98
KRLI+VL A LET K+ + PG RPDITHQCLL
Sbjct: 41 KRLIIVLEGAQLETVKVGHH-PGS------------------------CRPDITHQCLLM 75
Query: 99 LLDSPVNKAGKLQVYIQTSRGVLIELNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEK 158
L DSP+N+AG LQV+++T VLIE+NP RIPRTFKRF+GLMVQLLHK IR+ +S +
Sbjct: 76 LFDSPLNRAGLLQVFVRTEHNVLIEINPQTRIPRTFKRFAGLMVQLLHKFQIRANDSSRR 135
Query: 159 LLKVIKNPITDHLPTKCRKITLSFDAPVI-RVQDYVEKLDD-----DESICVFVGAMARG 212
L+ VIKNPITDH+P C+K +SF ++ +D V D+ DE + + +GA A G
Sbjct: 136 LMSVIKNPITDHVPVGCKKYAMSFSGKLLPNCRDLVPHGDETSASYDEPVVIVIGAFAHG 195
Query: 213 KDNFADEFVDEKIGLSNYPLSASVACSKFCHGCEDVWNIM 252
++ +E +SNYPLSA++ACSK C E+VW ++
Sbjct: 196 V--LKTDYTEELFSISNYPLSAAIACSKICSAFEEVWGVV 233
>Hs15625590 [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 151
Score = 160 bits (406), Expect = 1e-39
Identities = 80/154 (51%), Positives = 106/154 (67%), Gaps = 4/154 (2%)
Query: 99 LLDSPVNKAGKLQVYIQTSRGVLIELNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEK 158
L+DSP+N+AG LQVYI T + VLIE+NP RIPRTF RF GLMVQLLHKLS+R+ + +K
Sbjct: 2 LMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQK 61
Query: 159 LLKVIKNPITDHLPTKCRKITLSFDAPVIRVQDYVEKLDDDESICVFVGAMARGKDNFAD 218
LLKVIKNP++DH P C K+ SF PV V D E + + I VGA A GK +
Sbjct: 62 LLKVIKNPVSDHFPVGCMKVGTSFSIPV--VSDVRELVPSSDPIVFVVGAFAHGK--VSV 117
Query: 219 EFVDEKIGLSNYPLSASVACSKFCHGCEDVWNIM 252
E+ ++ + +SNYPLSA++ C+K E+VW ++
Sbjct: 118 EYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI 151
>ECU01g0480 [J] KOG3073 Protein required for 18S rRNA maturation and 40S
ribosome biogenesis
Length = 183
Score = 76.6 bits (187), Expect = 3e-14
Identities = 53/159 (33%), Positives = 84/159 (52%), Gaps = 7/159 (4%)
Query: 93 HQCLLTLLDSPVNKAGKLQVYIQTSRGVLIELNPTVRIPRTFKRFSGLMVQLLHKLSIRS 152
H+ L L+DSP+NK+ K+++YI T+R L+EL+ + +P S M LL ++ I+S
Sbjct: 31 HRALRLLMDSPLNKSKKMKIYIHTARNALVELSYLLEVPENPADLSDAMSYLLKRMVIKS 90
Query: 153 VNSEEKLLKVIKNPITDHLPTKCRKITLSFDAPVIRVQDYVEKLDDDESICVFVGAMARG 212
+ L KV+KNP+T+HLP KI LS + +D L E VF + R
Sbjct: 91 -SDGTMLGKVVKNPVTNHLPPNSTKIRLSPRGCKMSSKDLESSL---ERGFVFFIDVGRE 146
Query: 213 KDNFADEFVDEKIGLSNYPLSASVACSKFCHGCEDVWNI 251
++ EF + LS++ LS C+K + E++ I
Sbjct: 147 EEREEAEF---DMKLSDFKLSPESCCAKITNMFEELLQI 182
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,693,523
Number of Sequences: 60738
Number of extensions: 656727
Number of successful extensions: 1526
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1510
Number of HSP's gapped (non-prelim): 7
length of query: 252
length of database: 30,389,216
effective HSP length: 104
effective length of query: 148
effective length of database: 24,072,464
effective search space: 3562724672
effective search space used: 3562724672
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)