ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0507 good T KOG3873 Signal transduction mechanisms Sphingomyelinase family protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0507 170758 169409 -450
(450 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER019w [T] KOG3873 Sphingomyelinase family protein 516 e-146
SPBC685.10c [T] KOG3873 Sphingomyelinase family protein 187 3e-47
CE16515 [T] KOG3873 Sphingomyelinase family protein 110 3e-24
Hs4507095 [T] KOG3873 Sphingomyelinase family protein 108 2e-23
7292334 [T] KOG3873 Sphingomyelinase family protein 107 4e-23
>YER019w [T] KOG3873 Sphingomyelinase family protein
Length = 477
Score = 516 bits (1328), Expect = e-146
Identities = 258/438 (58%), Positives = 319/438 (71%), Gaps = 10/438 (2%)
Query: 15 IRFLTFNTWGLKYIARFRKQRLTALAERLAGNLDAGNFWDQ-----NPNDTKEQYDVVAL 69
I+FLTFNTWGLKY+++ RK+RL A+A++LAG+ D+ ++ E YDV+AL
Sbjct: 39 IKFLTFNTWGLKYVSKHRKERLRAIADKLAGHSMLTPISDELLPNGGDSNENEDYDVIAL 98
Query: 70 QEVWCDEDWNYIVERCKHRYPYYRRFKSGMISGPGLAILSKIPIESTFLYRFPINGRPSA 129
QE+WC EDW Y+ C +YPY R F SG+++GPGLAILSK+PIESTFLYRFPINGRPSA
Sbjct: 99 QEIWCVEDWKYLASACASKYPYQRLFHSGILTGPGLAILSKVPIESTFLYRFPINGRPSA 158
Query: 130 FWRGDWYVGKSISVTLLKRTSPEYPPLAILNSHMHAPYALTGDAAYECHRACQSWDFSKL 189
+RGDWYVGKSI++T+L + P+AI+NSHMHAPYA GDAAY CHR+CQ+WDFS+L
Sbjct: 159 VFRGDWYVGKSIAITVLNTGTR---PIAIMNSHMHAPYAKQGDAAYLCHRSCQAWDFSRL 215
Query: 190 VKLYKKAGYSVVVVGDLNSRPGSLPHKFLTIEAGQVDSWEQKFGVQDLKDIAALSPANQI 249
+KLY++AGY+V+VVGDLNSRPGSLPHKFLT EAG VDSWEQ G QDL IA LSP Q+
Sbjct: 216 IKLYRQAGYAVIVVGDLNSRPGSLPHKFLTQEAGLVDSWEQLHGKQDLAVIARLSPLQQL 275
Query: 250 TYGGTTCDSTLNTWRASRGAHEACRLDYALIDSNTLHTLDAKVTFTETIPNIG-SFSDHF 308
G TTCDS LNTWRA R EACRLDYALID + L T+DA V FTE IP++ S SDHF
Sbjct: 276 LKGCTTCDSLLNTWRAQRQPDEACRLDYALIDPDFLQTVDAGVRFTERIPHLDCSVSDHF 335
Query: 309 AYSIXXXXXXXXXXXXXPHILQETDHSMAKEQALERIQMYDDLLLCIDNYMATAKWQKLW 368
AYS P + + +E L+R Y+ ++ CI Y+ TA+ QK +
Sbjct: 336 AYSC-TLNIVPQGTESRPSTSVKRAKTHDRELILQRYSNYETMIECIHTYLKTAQRQKFF 394
Query: 369 RGTHFWVSIFFIIASMVVTTFTSNIAGWSSIFWILFSVALTASGLIDGLISLLFGNKEIR 428
RG HFW SI +IAS+VVTTFT+N AGWSSIFW+LF++A++ SG IDG IS LFG EIR
Sbjct: 395 RGLHFWASILLLIASLVVTTFTANKAGWSSIFWVLFAIAVSISGTIDGAISFLFGRSEIR 454
Query: 429 ALQEVYEEVSDAKRFLSS 446
AL EV +EV DA+ L +
Sbjct: 455 ALIEVEQEVLDAEHHLQT 472
>SPBC685.10c [T] KOG3873 Sphingomyelinase family protein
Length = 368
Score = 187 bits (475), Expect = 3e-47
Identities = 121/359 (33%), Positives = 176/359 (48%), Gaps = 29/359 (8%)
Query: 92 YRRFKSGMISGPGLAILSKIPIESTFLYRFPINGRPSAFWRGDWYVGKSISVTLLKRTSP 151
Y RF G GLA+ SK PI + + ++P+NGRP AFWRGDWYVGK ++ L+ S
Sbjct: 13 YSRFFHSAAMGAGLAMFSKFPIIESSMNKYPLNGRPQAFWRGDWYVGKGVATASLQHPSG 72
Query: 152 EYPPLAILNSHMHAPYALTGDAAYECHRACQSWDFSKLVKLYKKAGYSVVVVGDLNSRPG 211
+++ N+H+HAPY D Y CHR Q+W SKL++ + G+ V+ GD N +P
Sbjct: 73 RI--ISLFNTHLHAPYGKGAD-TYLCHRLSQAWYISKLLRAAVQRGHIVIAAGDFNIQPL 129
Query: 212 SLPHKFLTIEAGQVDSWEQKF--GVQDLKDIAALSPANQITYGGTTCDSTLNTWRASRGA 269
S+PH+ +T D+W + V+ + +++ + GTTCDS LNTWR + +
Sbjct: 130 SVPHEIITSYGLVNDAWLSVYPDQVEHPPNRFSMNDKELVEIAGTTCDSRLNTWRENISS 189
Query: 270 HE-----ACRLDYALIDSNTLHTLDAKVTFTETIPNIG-SFSDHFAYSIXXXXXXXXXXX 323
+ A RLDY +T +AKV F E +P + S+SDHFA
Sbjct: 190 KDMDDFVAKRLDYVFHSPSTCEAKNAKVVFLERVPKLDCSYSDHFAIE------TVLSIK 243
Query: 324 XXPHILQETDHSMAKEQALERIQMYDDLLLCIDNYMATAKWQKLWRGTHFWVSIFFIIAS 383
P +QET S + + DD L YMA + R H +SI II
Sbjct: 244 LQPIPVQETRVSYS---------IIDDTLGITYQYMARERLHMRLRIAHLLISIPLIIGV 294
Query: 384 MVVTTFTSNIAGWSSIFWILFSVALTASGLIDGL-ISLLFGNKEIRALQEVYEEVSDAK 441
V + W + + F+V LT + +++G I LLFG E L E E+ + K
Sbjct: 295 HVAIAWCD--PAWLKVIILFFTVMLTIAAVVNGFCIGLLFGRWEFNGLLEFVAELKEQK 351
>CE16515 [T] KOG3873 Sphingomyelinase family protein
Length = 445
Score = 110 bits (276), Expect = 3e-24
Identities = 75/250 (30%), Positives = 119/250 (47%), Gaps = 9/250 (3%)
Query: 62 EQYDVVALQEVWCDEDWNYIVERCKHRYPYYRRFKSGMISGPGLAILSKIPIESTFLYRF 121
E YD+V LQE+W D+ + E+ YPY+ F SG +G G+ + S+ PI ST R+
Sbjct: 74 ELYDIVGLQELWSYYDFVRLSEQVSSVYPYFHYFHSGF-TGSGVCVFSRHPIVSTLTNRY 132
Query: 122 PINGRPSAFWRGDWYVGKSISVTLLKRTSPEYPPLAILNSHMHAPYALTGDAAYECHRAC 181
+NG RGDW+ GK + +T ++ + +H+HA Y D Y HR
Sbjct: 133 SLNGFAHHIHRGDWFGGKVVGLTEIEIDGD--LRVNFYTTHLHAEYDREND-LYLPHRTA 189
Query: 182 QSWDFSKLVKLYKKAGYSVVVVGDLNSRPGSLPHKFLTIEAGQVDSWEQKFGVQDLKDIA 241
Q+++ ++ V+ + V+V GDLN P L + + A D+W V++
Sbjct: 190 QAFELAQFVRHTARGADVVIVTGDLNMEPCDLGFRLILSHAKLFDAWRMSHEVENEDSEG 249
Query: 242 ALSPANQITYGGTTCDSTLN--TWRASRGAHEACRLDYALIDSNTLHTL--DAKVTFTET 297
L I GG TCD N T RA + ++ R+DY L S + + ++T +
Sbjct: 250 ELLKFRGIAKGG-TCDRPDNCYTKRALKNVDDSKRIDYMLFKSGRCNVKLEECEITLNQI 308
Query: 298 IPNIGSFSDH 307
++SDH
Sbjct: 309 PGEDLNYSDH 318
>Hs4507095 [T] KOG3873 Sphingomyelinase family protein
Length = 423
Score = 108 bits (269), Expect = 2e-23
Identities = 62/203 (30%), Positives = 102/203 (49%), Gaps = 27/203 (13%)
Query: 14 RIRFLTFNTWGLKYIARFRKQRLTALAERLAGNLDAGNFWDQNPNDTKEQYDVVALQEVW 73
R+R N WG+ Y+++ R R+ L G+F +Q E +D+ L+EVW
Sbjct: 8 RLRIFNLNCWGIPYLSKHRADRMRRL----------GDFLNQ------ESFDLALLEEVW 51
Query: 74 CDEDWNYIVERCKHRYPYYRRFKSGMISGPGLAILSKIPIESTFLYRFPINGRPSAFWRG 133
++D+ Y+ ++ YP F+SG+I G GL + SK PI+ + + +NG P G
Sbjct: 52 SEQDFQYLRQKLSPTYPAAHHFRSGII-GSGLCVFSKHPIQELTQHIYTLNGYPYMIHHG 110
Query: 134 DWYVGKSISVTLLKRTSPEYPPLAILN---SHMHAPYALTGDAAYECHRACQSWDFSKLV 190
DW+ GK++ + +L + +LN +H+HA Y D Y HR Q+W+ ++ +
Sbjct: 111 DWFSGKAVGLLVLHLSG------MVLNAYVTHLHAEYNRQKD-IYLAHRVAQAWELAQFI 163
Query: 191 KLYKKAGYSVVVVGDLNSRPGSL 213
K V++ GDLN P L
Sbjct: 164 HHTSKKADVVLLCGDLNMHPEDL 186
>7292334 [T] KOG3873 Sphingomyelinase family protein
Length = 402
Score = 107 bits (267), Expect = 4e-23
Identities = 91/394 (23%), Positives = 166/394 (42%), Gaps = 49/394 (12%)
Query: 15 IRFLTFNTWGLKYIARFRKQRLTALAERLAGNLDAGNFWDQNPNDTKEQYDVVALQEVWC 74
+ LT N WG+ Y++ R+ R+ A+ + LA +YD+V+LQEVW
Sbjct: 6 LNILTLNIWGIPYVSSDRRPRIDAICKELASG----------------KYDIVSLQEVWA 49
Query: 75 DEDWNYIVERCKHRYPYYRRFKSGMISGPGLAILSKIPIESTFLYRFPINGRPSAFWRGD 134
ED + + + P+ F SG++ G GL +LSK PI T + + +NG D
Sbjct: 50 QEDSELLQKGTEAVLPHSHYFHSGVM-GAGLLVLSKYPILGTLFHAWSVNGYFHRIQHAD 108
Query: 135 WYVGKSISVTLLKRTSPEYPPLAILNSHMHAPYALTGDAAYECHRACQSWDFSKLVKLYK 194
W+ GK + L R + + N+H+HA Y D Y+ HR Q++D ++ ++ +
Sbjct: 109 WFGGKGVG---LCRILVGGQMVHLYNAHLHAEYDNAND-EYKTHRVIQAFDTAQFIEATR 164
Query: 195 KAGYSVVVVGDLNSRPGSLPHKFLTIEAGQVDSWEQKFGVQDLKDIAALSPANQITYGGT 254
++ GDLN++P + +K L + +DS + ++
Sbjct: 165 GNSALQILAGDLNAQPQDISYKVLLYTSKMLDSCDSD------------------SFRTN 206
Query: 255 TCDSTLNTWRASRGAHE-ACRLDYALI---DSNTLHTLDAKVTFTETIPNIG-SFSDHFA 309
C+ T + +R + R+D+ + D + K+ F E +P SFSDH A
Sbjct: 207 ECEHNSYTSKQARERNPLGIRIDHIFVRGGDHVNAEIAEYKLPFPERVPGEKFSFSDHEA 266
Query: 310 YSIXXXXXXXXXXXXXPHILQETDHSMAKEQALERIQMYDDLLLCIDNYMATAKWQKLWR 369
P E + + + + + L LC D + ++
Sbjct: 267 VMAKLKLFKLEPRSEEPVATIEVNCLVEDGETCSTAALLEALALC-DASLLQLNTDRI-- 323
Query: 370 GTHFWVSIFFIIASMVVTTFTSNIAGWSSIFWIL 403
++ + F + +++ FT+ + G +IF +L
Sbjct: 324 -LYYSAATFLFVLLVLLVEFTAPV-GMRTIFLLL 355
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.136 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,659,901
Number of Sequences: 60738
Number of extensions: 1098245
Number of successful extensions: 2550
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2532
Number of HSP's gapped (non-prelim): 5
length of query: 450
length of database: 30,389,216
effective HSP length: 110
effective length of query: 340
effective length of database: 23,708,036
effective search space: 8060732240
effective search space used: 8060732240
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)