ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0516 good S KOG2970 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0516 172317 173348 344
(344 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR044c [S] KOG2970 Predicted membrane protein 336 3e-92
SPAC823.07 [S] KOG2970 Predicted membrane protein 152 7e-37
7302283 [S] KOG2970 Predicted membrane protein 134 1e-31
At1g16560 [S] KOG2970 Predicted membrane protein 125 9e-29
CE18091 [S] KOG2970 Predicted membrane protein 97 3e-20
ECU09g1170 [S] KOG2970 Predicted membrane protein 76 6e-14
At5g62130 [S] KOG2970 Predicted membrane protein 74 2e-13
>YCR044c [S] KOG2970 Predicted membrane protein
Length = 357
Score = 336 bits (862), Expect = 3e-92
Identities = 175/342 (51%), Positives = 219/342 (63%), Gaps = 22/342 (6%)
Query: 16 YVSLASPGDRLPEFSDCVRACEVVRHCTD---RFEEDSFNPFIN-EQFSEPALVYKALLW 71
++ SPGD L EF DC ACE R C + + + N F + E F P L K L W
Sbjct: 14 FLVTCSPGDNLDEFIDCTYACEYNRRCPNSQINYIDPETNMFHDIEFFDTPPLYSKLLFW 73
Query: 72 DCTSDCDYQCQQKITLDREERNEDIYQFHGKWPFKRVLGMQEFYSTIFSICNFVPHYRGF 131
DC SDCDYQCQ IT R + E+IYQFHGKWPF RVLG QEF+STIFSI NF+PHY+GF
Sbjct: 74 DCISDCDYQCQHIITRWRIDEEEEIYQFHGKWPFLRVLGTQEFFSTIFSIGNFIPHYKGF 133
Query: 132 ----KLARKSLAKLQKTSQRRVLILNYIFISMAGMIAWICSSIFHTRDLIITEKLDYVFA 187
++ R+ + +K S R +LI NY+++++AGM+AW SS+FH RDLIITEKLDY FA
Sbjct: 134 VKFSRIIREEGDRRRKNS-RSILIWNYLYVTVAGMLAWTASSVFHCRDLIITEKLDYFFA 192
Query: 188 GATVLSGFHGVFYRVARLDLHPRVGALFSLSVFTIFVGHLLRLYLNWSYAYNMRFNIFFG 247
G TVL+GFH +F R+ + L+P++ F+ SV IF H+LRLY++WSY YNMRFNIFFG
Sbjct: 193 GLTVLTGFHAIFARMTSMFLYPKIAQAFTASVAAIFALHILRLYVDWSYTYNMRFNIFFG 252
Query: 248 LLQYILLITLAILNY-------------RTFSSIRPDXXXXXXXXXXXXXXXXXXAMSSE 294
+LQYILLI L+ NY +T S AMS E
Sbjct: 253 VLQYILLIMLSCQNYHALQKQKLMGEFKKTAYSSFKRQIFKLCVIPILLVIVTTMAMSLE 312
Query: 295 LFDFFSYRWQIDSHAIWHALTIVPSFYLYEFFLKDYHVLGSR 336
LFDFFSY WQID+HA+WH TI PS+ LY+FFL+DY G+R
Sbjct: 313 LFDFFSYEWQIDAHALWHLCTIWPSWVLYDFFLEDYAYWGNR 354
>SPAC823.07 [S] KOG2970 Predicted membrane protein
Length = 331
Score = 152 bits (384), Expect = 7e-37
Identities = 108/326 (33%), Positives = 151/326 (46%), Gaps = 38/326 (11%)
Query: 11 LSFFVYVSLASPGDRLPEFSDCVRACEVVRHCTDRFEEDSFNPFINEQFSEPALVYKALL 70
LS F +S AS GD P + CV C + C + S P L K
Sbjct: 16 LSLFRQIS-ASAGDLHPVYVSCVNRC-IENKCHGNPSDTSKLP----------LDLKLFR 63
Query: 71 WDCTSDCDYQCQQKITLDREERNEDIYQFHGKWPFKRVLGMQEFYSTIFSICNFVPHYRG 130
WDC S+C Y+C+ N Q+HGKW F RV G+QE +S FS+ NF+ HY G
Sbjct: 64 WDCGSNCGYECEITAENYFAAHNLPSQQYHGKWYFIRVFGIQELFSVFFSMLNFMIHYNG 123
Query: 131 FKLARKSLAKLQKTSQRRVLILNYIFISMAGMIAWICSSIFHTRDLIITEKLDYVFAGAT 190
+ + R+ + + L L++ + GM AW+ SS+FH RD ITEKLDY AGA
Sbjct: 124 YHIMRRCIPDEHPAKR---LCLSWAIV---GMNAWVWSSVFHIRDTPITEKLDYFSAGAF 177
Query: 191 VLSGFHGVFYRVARLDLHPR-------VGALFSLSVFTIFVGHLLRL-YLNWSYAYNMRF 242
VL G + + RLD P +G +F F+ H+ L + ++ Y YNM+
Sbjct: 178 VLFGSYCTLILMLRLDQLPGGKLLCWIIGVIF----IAAFIAHVSYLSFYSFDYGYNMKA 233
Query: 243 NIFFGLLQYILLITLAILNYRTFSSIRPDXXXXXXXXXXXXXXXXXXAMSSELFDFFSYR 302
N+ GL+Q IL + +S + A S ELFDF
Sbjct: 234 NVAVGLVQNIL--------WYYYSWSNRNSGLYWTRWPAYIVTSLMLATSLELFDFSPIA 285
Query: 303 WQIDSHAIWHALTIVPSFYLYEFFLK 328
ID+HA+WH T+ + YLY F ++
Sbjct: 286 NLIDAHALWHLSTVPITHYLYGFVVR 311
>7302283 [S] KOG2970 Predicted membrane protein
Length = 326
Score = 134 bits (338), Expect = 1e-31
Identities = 104/320 (32%), Positives = 150/320 (46%), Gaps = 31/320 (9%)
Query: 11 LSFFVYVSLASPGDRLPEFSDCVRACEVVRHCTDRFEEDSFNPFINEQ---FSEPALVYK 67
L V LAS GDR F +C + CE D E I EQ F + ++ +
Sbjct: 13 LGALVTACLASNGDRTQFFHNCRQNCERTNCSADGLE-------IQEQAVKFYQQSVFDR 65
Query: 68 ALLWDCTSDCDYQCQQKITLDREERNEDIYQFHGKWPFKRVLGMQEFYSTIFSICNFVPH 127
W C +C Y C + ER I QF+GKWPF R+LGMQE S IFS NFV H
Sbjct: 66 LFQWSCADECQYGCMWRTVFAFFERGWPIPQFYGKWPFLRLLGMQEPASVIFSCLNFVVH 125
Query: 128 YRGFKLARKSLAKLQKTSQRRVLILNYIFISMAGMIAWICSSIFHTRDLIITEKLDYVFA 187
R L RK +++ S +L +IF ++ + WI S+IFHTRD +TE LDY FA
Sbjct: 126 LR---LLRKFRREVRPDSP--CYMLTHIF-AVTSLNGWIWSAIFHTRDFPLTELLDYAFA 179
Query: 188 GATVLSGFHGVFYRVA-RLDLHPRVGALFSLSVFTIFVGHLLRLYLN-WSYAYNMRFNIF 245
+ +L + + R+ R L R + +L+ + ++ + L + ++YA+NM N+
Sbjct: 180 YSIILCSLYVMVMRMLHRYSLFLR--GVITLAFLSYYINYFAYLSVGRFNYAFNMMVNVA 237
Query: 246 FGLLQYI-LLITLAILNYRTFSSIRPDXXXXXXXXXXXXXXXXXXAMSSELFDFFSYRWQ 304
G++ + + + R RP AMS EL DF W
Sbjct: 238 TGVIAAVGWFVWCHFVRTR-----RP-----YFRRILRFYILMALAMSLELLDFPPILWI 287
Query: 305 IDSHAIWHALTIVPSFYLYE 324
+D+HA+WH TI + YE
Sbjct: 288 LDAHALWHLATIPLASLYYE 307
>At1g16560 [S] KOG2970 Predicted membrane protein
Length = 342
Score = 125 bits (314), Expect = 9e-29
Identities = 92/324 (28%), Positives = 148/324 (45%), Gaps = 29/324 (8%)
Query: 17 VSLASPGDRLPEFSDCVRACEVVRHCTDR--FEEDSFNPFINEQFSEPALVYKALLWDCT 74
+S AS GD P++ CV CE+ C + F + + + + + L + W C
Sbjct: 19 ISNASAGDADPDYRTCVSECEI-SGCVGQLCFPQCNSSSDGGPWYIQEPLYLQWKKWGCQ 77
Query: 75 SDCDYQCQQKITLDREERNEDIYQFHGKWPFKRVLGMQEFYSTIFSICNFVPHYRGF--- 131
DC YQC +RE + ++HGKWPFKRVLG+QE S FS+ N H+ G+
Sbjct: 78 GDCRYQCMVNRETERETLGQAPVKYHGKWPFKRVLGIQEPASVAFSVLNLAMHFHGWLSF 137
Query: 132 --KLARKSLAKLQKTSQRRVLILNYIFISMAGMIAWICSSIFHTRDLIITEKLDYVFAGA 189
+ K K +T+ + L +I+ + M +W S++FH+RD+ +TE+LDY + A
Sbjct: 138 FIMIYYKLPLKQDRTAYYEYVGLWHIY-GLLSMNSWFWSAVFHSRDVDLTERLDY--SSA 194
Query: 190 TVLSGFHGVFYRVARLDLHPRVG-ALFSLSVFTIFVGHLLRLYLNW---SYAYNMRFNIF 245
+ GF + + D+ + S + H+L Y+N+ Y +NM +
Sbjct: 195 VAILGFSLILAILRTFDIRVEAARVMVSAPILAFVTTHIL--YINFYKLDYGWNMIVCVA 252
Query: 246 FGLLQYILLITLAILNYRTFSSIRPDXXXXXXXXXXXXXXXXXXAMSSELFDFFSYRWQI 305
G+ Q L A ++ + AM E++DF Y
Sbjct: 253 MGVSQLFLWARWAAVSSHPSN-----------WKLWVVVIAGGLAMLLEIYDFPPYEGYF 301
Query: 306 DSHAIWHALTIVPSFYLYEFFLKD 329
D+H+IWHA TI P L+ F++D
Sbjct: 302 DAHSIWHAATI-PLTILWWSFIRD 324
>CE18091 [S] KOG2970 Predicted membrane protein
Length = 320
Score = 97.1 bits (240), Expect = 3e-20
Identities = 87/333 (26%), Positives = 133/333 (39%), Gaps = 44/333 (13%)
Query: 8 LACLSFFVYVSL-ASPGDRLPEFSDCVRACEVVRHCTDRFEEDSFNPFINEQFSEPALVY 66
+ CL +V L ASPGDR + +C + C +C+ +F
Sbjct: 7 VGCLLITCFVQLEASPGDRSIWYQECTQVCISKYNCSTKFG------------------- 47
Query: 67 KALLW---DCTSDCDYQCQQKITLDREERNEDIYQFHGKWPFKRV-----LGMQEFYSTI 118
L W DC C Y C + + QFHGKWPF + +QE S I
Sbjct: 48 -TLDWARGDCFW-CRYDCMWDTIGHFDSNFGVVPQFHGKWPFLAIPLPFGFIIQEPASMI 105
Query: 119 FSICNFVPHYRGFKLARKSLAKLQKTSQRRVLILNYIFISMAGMIAWICSSIFHTRDLII 178
FS+ N Y K+ R+ K+Q R + ++ + GM WI SS+FH D
Sbjct: 106 FSLLNLFTVY---KMLRR-FKKMQNLPNRTM----WLVYAHVGMFTWISSSLFHMFDCDF 157
Query: 179 TEKLDYVFAGATVLSGFHGVFYRVARLDLHPRVGALFSLSVFT-IFVGHLLRLYLNWSYA 237
TEK+DY A + VL + +L R G + +F +++ H +++ N+ Y
Sbjct: 158 TEKMDYFGAYSFVLFALYVSVIFTKQLQFLGRGGPKYIQILFALVYLNHFMKMMQNFDYG 217
Query: 238 YNMRFNIFFGLLQYILLITLAILNYRTFSSIRPDXXXXXXXXXXXXXXXXXXAMSSELFD 297
YNM I F L+ L + R S++ + + E+ D
Sbjct: 218 YNMTCCIVFSLITTCLYVHHLYYRKRNLGSLQ-----ESDIVLIRLIIWANLSTALEILD 272
Query: 298 FFSYRWQIDSHAIWHALTIVPSFYLYEFFLKDY 330
F W DSH+++H TI + +F Y
Sbjct: 273 FTPVFWIFDSHSLFHLATIPIPIWWSDFLALYY 305
>ECU09g1170 [S] KOG2970 Predicted membrane protein
Length = 274
Score = 76.3 bits (186), Expect = 6e-14
Identities = 62/231 (26%), Positives = 105/231 (44%), Gaps = 27/231 (11%)
Query: 100 HGKWPFKRVLGMQEFYSTIFSICNFVPHYRGFKLARKSLAKLQKTSQRRVLILNYIFISM 159
+G+W F+ +LGM EF+S +FS N + + F K L+ T R+ + Y +
Sbjct: 53 NGRWGFQPILGMTEFFSALFSFMNLITNIICFHRMLKK--HLRVTRLGRLYYIQYYICN- 109
Query: 160 AGMIAWICSSIFHTRDLIITEKLDYVFAGATVLSGFHGVFYRVARLDLHPRVGALFSLSV 219
+A+I S++FH + T DY A T+L GF+ RV + S S+
Sbjct: 110 ---LAFISSTLFHIHENTFTRNCDYFLAFLTILFGFYMALVRVI---------LIVSPSI 157
Query: 220 FTIFVGHLLRLYLNWSYAY------NMRFNIFFGLLQYILLITLAILNYR-TFSSIRPDX 272
G L +++ + YAY N+ F+ + + +++TL +L++ TF R
Sbjct: 158 EKATRGPLQAIFILF-YAYHIHRMSNIEFDYVYNKISCAIIVTLTLLSHLVTFLKYR--- 213
Query: 273 XXXXXXXXXXXXXXXXXAMSSELFDFFSYRWQIDSHAIWHALTIVPS-FYL 322
A + E+ D Y + +DSHAIWH ++ + + FYL
Sbjct: 214 KLAHTKHILFFTFFFFLAGAIEIQDVPPYAYLVDSHAIWHLISCISTPFYL 264
>At5g62130 [S] KOG2970 Predicted membrane protein
Length = 253
Score = 74.3 bits (181), Expect = 2e-13
Identities = 70/262 (26%), Positives = 117/262 (43%), Gaps = 24/262 (9%)
Query: 88 DREERNEDIYQFHGKWPFKRVLGMQEFYSTIFSICNFVPHYRGF------KLARKSLAKL 141
+R+ E ++ GKWP K V G+QE S FS + ++G+ + L
Sbjct: 6 ERKRNGERPTKYFGKWPLKHVYGIQEPVSVAFSALDLAMQFQGWVSYFILVYYKLPLQPN 65
Query: 142 QKTSQRRVLILNYIFISMAGMIAWICSSIFHTRDLIITEKLDYVFAGATVLSGFHGVFYR 201
+KT I++ I + + W SSI H+RD+ +TE+LDY + ATVL+GF +
Sbjct: 66 RKTYYEYNGIVHIYAIIVMNSLFW--SSICHSRDVELTERLDY--SSATVLAGFSLILAI 121
Query: 202 VARLDLHPR-VGALFSLSVFTIFVGHLLRLYLNWSYAYNMRFNIFFGLLQYILLITLAIL 260
+ + + V + + + + H+ LYLN+ YN+ + + ++ I I L +
Sbjct: 122 LRSFSIQDQSVKIMVTAPILAVVATHI--LYLNF---YNLDEGLHWKVIFGIGGIELVVW 176
Query: 261 NYRTFSSIRPDXXXXXXXXXXXXXXXXXXAMSSELFDFFSYRWQIDSHAIWHALTIVPSF 320
+ P + +FDF Y+ ID+HA+W I P
Sbjct: 177 GLWAALTSHP-----SKWKLRAFLISSILTLCLRMFDFPPYKGYIDAHALWRGAGI-PLS 230
Query: 321 YLYEFFLKDYHVLGSRTSTNIK 342
YL+ F+ D V RT+ N+K
Sbjct: 231 YLWWSFVCDDAVF--RTTVNLK 250
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.331 0.143 0.454
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,169,590
Number of Sequences: 60738
Number of extensions: 758911
Number of successful extensions: 1905
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1880
Number of HSP's gapped (non-prelim): 11
length of query: 344
length of database: 30,389,216
effective HSP length: 107
effective length of query: 237
effective length of database: 23,890,250
effective search space: 5661989250
effective search space used: 5661989250
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)