ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0567 suspect: LH L KOG1974 Replication, recombination and repair DNA topoisomerase I-interacting protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0567 189083 192568 1162
(1162 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL273w [L] KOG1974 DNA topoisomerase I-interacting protein 871 0.0
SPBC216.06c [L] KOG1974 DNA topoisomerase I-interacting protein 308 3e-83
>YNL273w [L] KOG1974 DNA topoisomerase I-interacting protein
Length = 1238
Score = 871 bits (2251), Expect = 0.0
Identities = 481/1167 (41%), Positives = 727/1167 (62%), Gaps = 51/1167 (4%)
Query: 29 DISYTIIKARIGMLATAIGGPDHSVDSVNPPYKVGDDCLACIKDLIRWFKLVDDNQKRWD 88
D S T+++ARI +LATAIGGPD++ PPYK+GDDCLAC+KDL RWFKLVDD QKRWD
Sbjct: 14 DFSLTVLRARIALLATAIGGPDYTSQIDPPPYKLGDDCLACLKDLKRWFKLVDDQQKRWD 73
Query: 89 VEMATAEFKILQNDLIPILLDWEAKNSAATRKSKKTGEDISIFFPNKSYHDRIALGALQL 148
V MA AE++IL +DL+PIL+DWE K S A + +K + F NK+Y+D+IAL LQL
Sbjct: 74 VAMAVAEYRILTDDLLPILIDWENKCSLAAKLAKNNPDHEE--FRNKAYYDKIALNCLQL 131
Query: 149 MVLMTWPLIITDQSSYNQVNYYFELKKHQLLYKHAILTTENGKVLKAAIRLALNVMSVDV 208
+VLMTWPLI+T+QSS NQ+ Y ELKKHQL+YK IL+ E+GKVL+AAIRLAL+V+ +D
Sbjct: 132 LVLMTWPLIVTEQSSSNQITLYGELKKHQLVYKKTILSMESGKVLRAAIRLALDVIKIDR 191
Query: 209 DHRTARDDSLIRMVLNFLKNVVAIEPGEVTISSVKRL-KRPLTMAEMLPTNITVDDISIN 267
RT RD+ ++++VLNF +NV+AIEPGE TI++ K + K+ +T + LP N+++DDIS+N
Sbjct: 192 LSRTPRDNMVLKLVLNFFRNVIAIEPGEFTINTKKSMPKKGITSIDTLPPNVSMDDISLN 251
Query: 268 SVITAFDKNKVFGFLLTVASSLSDAVDPNFVSWPLLEVMFFLTKDINPIRLFKNQRKSYK 327
+VI++F KNKVFGFLLT+ SSLS D +F++ PLLE+MF+ TKD+N LF Q ++
Sbjct: 252 TVISSFHKNKVFGFLLTLTSSLSKEFDQDFINIPLLEIMFYFTKDVNQELLFPRQ---FE 308
Query: 328 LGTDHEITDSN--------MTTSGKHLSELLAKEHEKKLNVIKNTSSRHSRFGGLLSIQT 379
GT ++ + N +T++G LS+LL KEH+ + NVIK+TS+RHSRFGGLLSIQT
Sbjct: 309 TGTHSKVVNKNESSSANNIVTSAGFELSKLLQKEHQMRKNVIKHTSARHSRFGGLLSIQT 368
Query: 380 PQNTRLTIASNSVNMRDDAALQELDSRKKWNKTITMRLDVI--EGLSSSFFSTE-GNSIY 436
P TRLT++ + + + ALQ+LD KKWNK I + + EGL +S +++ G +I+
Sbjct: 369 PDKTRLTVSGSQALVDEKIALQKLDDSKKWNKRIIKKHQSVAAEGLPNSLLNSQTGKAIF 428
Query: 437 MSSDNITSIKEFLADFVDSSFNLLLQNATDSFTSEIQDQLPLHKIEYMLFISWFVKFQRS 496
+ N KEF+ +F+DS FN+LL + T+ FT+E + L ++EY+LF +WFVK+Q
Sbjct: 429 FTESNGKHFKEFINNFIDSGFNILLHSVTNYFTTEQDRMVTLEQVEYLLFFAWFVKYQLL 488
Query: 497 RCIYEIDATPDYVSGALLDECYILFTKYLRESYEQKNWPVVHAGMLLFTEYLEFLLSLDQ 556
R + A VS AL + +IL + LR +Y+ KNW V HAGM+ F E L +
Sbjct: 489 RSKIDNSADIKQVSEALKEVTFILVSSLLRSAYDLKNWTVTHAGMIAFNELLNLVSRTKA 548
Query: 557 SWE---ADVQAVISKILSENMLQLLASLPKSATSHSSQYVKACINLTHVVLKTIDKFDEN 613
+ E D++ ++S++ S+ +QLL++LPK + +S Q++K+CI LTH VLK ++++ ++
Sbjct: 549 AQEEDSTDIEFIVSRLFSDERIQLLSNLPKIGSKYSLQFMKSCIELTHSVLKVLEQYSDD 608
Query: 614 SSLTVE--SKRKRKVNLNHTAIEKYAKDNDLDYESAFDILEEQFKQVTINFDKVFRGYLT 671
+L +E S+R++K N++ I K ++ ++D + A DIL + + +NF KV Y+T
Sbjct: 609 KTLVIEGKSRRQKKFNISEGDITKLIEEENVDRDEALDILTSSLRSIEVNFQKVQANYMT 668
Query: 672 EPTISTYIRYLQSYKELEDKDLIRVLKFFQRVFVKAKEELFLFRIDFMILCREILSQQGL 731
EP I TYI +L+ ++ELED + +V FF RVFV+AKE+ LFR D +IL RE+LS GL
Sbjct: 669 EPVIETYINFLERFRELEDDSIKKVFSFFHRVFVQAKEQALLFRFDLIILLREMLSPDGL 728
Query: 732 PTTSELRTHFTKFNEYYXXXXXXXXXXMPSLYINILFPMLHDSQISYYMRHGKMKLTEGT 791
S R + ++F++Y+ P+ ++ +LFP LH+S++ +Y R+G+ +
Sbjct: 729 DRMSRSRKYVSQFSDYFLARLKKRLKKSPAWFVGLLFPPLHNSEVGFYQRYGEYNVLNNE 788
Query: 792 PDIVLP-STFINIPDEGSLPKKVLLDMQVGILVSSLIDDGYEDLIEALLLNLQSSFDGLK 850
P S F IPDE +LP +LLDM+ G+LVS+L+DDG +L++ LL ++ + D K
Sbjct: 789 SMYAAPASQFKPIPDEEALPPSILLDMKYGVLVSTLLDDGKTELLDQLLKHITHTLDIFK 848
Query: 851 GKVAEGINSLEEE-RVKKVAFNATNSEIKRALYQQPEFRRLLILAGFAIPEANQPACYFI 909
+ +N+ +E F T +++ ++R LL+L G++IP C+
Sbjct: 849 SWLTVNVNAGKETVNPPNEYFTLTGVLNNDPIFKDKDYRALLLLIGYSIPRKINEPCFLP 908
Query: 910 NNKTPTEVQLVIETIQKHRSLPFESEIGKPASYYL---------SSHYQ----------E 950
+++ + E ++K+ S PFE+ G P+S YL SH + +
Sbjct: 909 GTVEVSDLTVSCELVKKYLSTPFETPNGLPSSSYLLRVRSEKDSFSHNEQDGWEGDDDYD 968
Query: 951 YEDPEEYGSD----LNNGSYFEDLE-IMDKRTEGRELSKGKAQSKLV-QRSKGKRKAIAH 1004
Y DP D ++ +YF+DL+ + +G + SKG A+SK +R + K +A +
Sbjct: 969 YNDPYIVPDDQILSKSDAAYFKDLDNNASDKLKGTKFSKGIARSKKKDKRKRRKGEAKTN 1028
Query: 1005 HPEENESDFEANASSSTKLPVVSKEYIMDSDDEDPDFGAIFFENETYLRQLLDKHNGSLT 1064
P + D E + + V SKE+I DS+D++ IFFENETY+R LLDK+NG LT
Sbjct: 1029 LPMFGDQDDERPQTVRERHGVFSKEFISDSEDDEDLMNPIFFENETYMRWLLDKNNGQLT 1088
Query: 1065 ETQFSLFAQFCEERVKNNGQLRNDYSALFNETPKSPIFSDVDAETIIIGSASLPSLANE- 1123
E ++ FA+F ER+ N G + DY++LF + S + SL SLA+
Sbjct: 1089 EDRYIQFAKFAAERMNNGGVVTGDYTSLFGGSIPSIESIRATESSSFAPDKSLISLASHV 1148
Query: 1124 PIETQVVSSEEYSSHPL-EEEVTSEQR 1149
E + +++ L +++V SE R
Sbjct: 1149 ASEMSIFDVNNNNNNQLSDDDVNSESR 1175
>SPBC216.06c [L] KOG1974 DNA topoisomerase I-interacting protein
Length = 971
Score = 308 bits (789), Expect = 3e-83
Identities = 280/1044 (26%), Positives = 471/1044 (44%), Gaps = 148/1044 (14%)
Query: 42 LATAIGGPDHSVDSVNPPYKVGDDCLACIKDLIRWFKLVDDNQKRWDVEMATAEFKILQN 101
+ +AIGG D+S D Y +GD+ LAC+KDL R+ ++VD+ K W + + +++ N
Sbjct: 10 IVSAIGGFDYSDDE--KVYVLGDEALACLKDLKRYLQVVDEKYKVWQIRSLLSSLQLVTN 67
Query: 102 DLIPILLDWEAKNSAATRKSKKTGEDISIFFPNKSYHD-RIALGALQLMVLMTWPLIITD 160
D+ PIL DW+ +DI+ +Y + RIAL ++L+V +TWPL
Sbjct: 68 DICPILSDWD--------------KDIT------NYRNWRIALACVELLVPLTWPLETEH 107
Query: 161 QSSYNQVNYYFELKKHQLLYKHAILTTENGKVLKAAIRLALNVMSVDVDHRTARDDSLIR 220
++ V+ + L++ Q YK++IL+ + G VL A + + L +S + RT RD +IR
Sbjct: 108 ETFRENVDVLYNLRQAQSNYKNSILSYKKGSVLSAILAVLLKPLSTPAESRTLRDKGIIR 167
Query: 221 MVLNFLKNVVAIEPGEVTISSVKRLKRPLTMAEMLPTNITVDDISINSVITAFDKNKVFG 280
+VL +N++ I+ LK + N I +F K +
Sbjct: 168 IVLLLFRNILQID----------ELK------------------TKNETIISFAKAHILD 199
Query: 281 FLLTVASSLSDAVDPNFVSWPLLEVMFFLTKDINPIRLFKNQRKSYKLGTDHEITDSNMT 340
++T+ S+L + +LE+++ L + P LF +D+++T
Sbjct: 200 LIVTLVSNLDEF---EHFDVYILEIVYNLIRGCKPSALF---------------SDASLT 241
Query: 341 TSGKHLSELLAKEHEKKLNVIKNTSSRHSRFGGLLSIQTPQNTRLTIASNSVNMRDDAAL 400
S L+ LL KE + + +N +RH+RFG +LS+QT ++ R TIAS N++ D L
Sbjct: 242 NSQTELNSLLLKESTQNRYLKRNAHTRHNRFGTMLSVQT-EDRRFTIASQ--NIKTD-GL 297
Query: 401 QELDSRKKWNKTITMRLDVIEGLSSSFFSTEGNSIYMSSDNITSIKEFLADFVDSSFNLL 460
ELDS K++ K T R F S +++++ T+++ F +F+++ FN L
Sbjct: 298 DELDSHKRFRKRGTRR---------KHFDDINKSFFINTEAGTALRNFAVEFLEAGFNPL 348
Query: 461 LQNATDSFTSEIQDQLPLHKIEYMLFISWFVKFQR-------SRCIYEIDATPDYVSGAL 513
Q+ E LP+HK++ + S+F++F R + IY D + +
Sbjct: 349 FQSLLKDLEREDPRVLPIHKMQLLYVQSFFLEFMRFSSKPKKTEEIYSNDYSFGLAASVF 408
Query: 514 LDECYILFTKYLRESYEQKNWPVVHAGMLLFTEYLEFLLSLDQSWEADVQAVISKILS-- 571
I+ + + ES+E K W A ML T+ L L S+ Q + +LS
Sbjct: 409 DQRALIMHNRLMVESFEMKQWSTFQASMLSMTQLLFTLRSMTLCSSEIYQRIADNLLSNI 468
Query: 572 ---ENMLQLLASLPKSATSHSSQYVKACINLTHVVLKTIDKFDENSSLTVESKRKRKVNL 628
E +L L+ S K + S Y+ A LT V+LK ++KF KR+R
Sbjct: 469 FYQEEILLLVYSALKHFKTQSFGYLDAITELTIVLLKELEKFSSAKQYLYVKKRRR---- 524
Query: 629 NHTAIEKYAKDNDLDYESAFDILEEQFKQVTINFDKVFRGYLTEPTISTYIRYLQSYKEL 688
N +++ ++D D ES+ + +F + Y I +++ +LQ Y++L
Sbjct: 525 NQKSVDSNVLESDEDEESSLINANAAVEDRLFDFGRYESRYCDNGCIDSFVLFLQCYQDL 584
Query: 689 EDKDLIRVLKFFQRVFVKAKEELFLFRIDFM-ILCREILSQQGLPTTSELRTHFTKFNEY 747
+ K + R + FF R+FVK K ++L+R+DF+ +L + TT+ R F +F Y
Sbjct: 585 DSKQIHRAISFFYRIFVKQKCHVYLYRLDFLRVLDKMFNDHVYFSTTNSARQDFEQFFVY 644
Query: 748 YXXXXXXXXXXMPSLYINILFPMLHDSQISYYMRHGKMKL--TEGTPDIVLPSTFINIPD 805
Y +P+L+I + FP L D+ YY+ +GK L G+ L T +P
Sbjct: 645 YMRKLSDALKDVPALFIELPFPKLTDT--FYYLEYGKSPLFSIHGSRKGPLYET---VPG 699
Query: 806 EGSLPKKVLLDMQVGILVSSLIDDGYEDLIEALLLNLQSSFDGLK--------GKVAEGI 857
L K V +V+ LI++ DL++ L + L K + EG
Sbjct: 700 LSHLEK-------VAAVVACLINENKSDLLDELKVQLNCLISERKLITLADENKYINEGG 752
Query: 858 NSLEEERVKKVAFNATNSEIKRALYQQPEFRRLLILAGFAIPEANQPACYFINNKTPTEV 917
N + ER+ K T+S AL + +FR LL L GF + N K P V
Sbjct: 753 N--DGERMGKNLKGDTDS-FNTALLKDGKFRLLLELCGFEESDNNIDVQALW--KLPNSV 807
Query: 918 QLVIETIQKHRSL-------PFESEIGKPASYYLSSHYQEYEDPEEYGSDLNNGSYFEDL 970
+I+ + +H L P E KP + P + ++ +
Sbjct: 808 --IIDELVEHAMLLRRFTDDPPTFEGTKPEDLLVRKQRGNVRLPSSSEGETSDEEI--EF 863
Query: 971 EIMDKRTEGRELSKGKAQSKLVQRSKGKRKAIAHHPEENESDFEANASSSTKLPVVSKEY 1030
E D T ++ +A +K+ R + K K NE+ + + + S +Y
Sbjct: 864 EADDPITFA---NRREALNKITDRKRKKMKT-------NETIIDHTTRKKKENHLRSAKY 913
Query: 1031 IMDSDDEDPDFGAIFFENETYLRQ 1054
I+DSDD D + FF++E LR+
Sbjct: 914 IVDSDD-DSETDIAFFQSEAALRE 936
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,481,168
Number of Sequences: 60738
Number of extensions: 2946899
Number of successful extensions: 9422
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 9399
Number of HSP's gapped (non-prelim): 4
length of query: 1162
length of database: 30,389,216
effective HSP length: 117
effective length of query: 1045
effective length of database: 23,282,870
effective search space: 24330599150
effective search space used: 24330599150
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)